32results about How to "Excellent strain" patented technology

The invention discloses Saccharomyces cerevisiae genetic engineering bacteria with high ester yield. The Saccharomyces cerevisiae genetic engineering bacteria EY-13 were collected in China General Microbiological Culture Collection Center on November 17, 2010, the collection number is CGMCC NO.4350, and the Saccharomyces cerevisiae genetic engineering bacteria are recommended to be named Saccharomyces cerevisiae. PGK1 derived from the Saccharomyces cerevisiae is selected as a promoter; and the construction method of the Saccharomyces cerevisiae genetic engineering bacteria with the high esteryield comprises a step of knocking-out an IAH1 gene for encoding ester hydrolase in a Saccharomyces cerevisiae genome when an ATF1 gene which is derived from the Saccharomyces cerevisiae and is used for encoding alcohol acetyltransferase is overexpressed. Compared with initial recipient bacteria, the Saccharomyces cerevisiae genetic engineering bacteria have the advantages that: after rice wine fermentation is simulated, the content of isoamylol is about one half, the content of ethyl acetate is improved by 20 times, the content of isoamyl acetate is 100mg / L, and the content of isobutyl acetate is 5 to 7mg / L; and after liquor fermentation is simulated, the content of total esters is improved by 4 times and the content of the ethyl acetate is improved by 35 times, so an excellent strain is provided for the production of brewing industry.

The invention provides an industrialized production method of cordyceps mushroom. The process of the invention comprises superior strain and liquid strain production, strain inspection, strain preservation, cordyceps mushroom culture equipment, culture medium formulary, culture medium preparation, culture medium subpackage, inoculation method, hairy fungus grass outgrowth management, collection and dry process, etc. The invention has the advantages that the technological innovation points are more, the invention is advanced and practical, the mechanization degree is high, and the invention is suitable for the scale and industrialized production. The selected superior strain has stronge prolificacy, the veraison is quick, and grass outgrowth is easy, the weed resistance and the stress resistance are outstanding, the invention is served with advanced liquid strain production equipment, semisolid culturemedium and case and bowl incubator, and other advanced production technologies, and can ensure the cordyceps mushroom which is a new member of the worm grass family to realize the high yield rate, the high quality and the high efficiency during the scale planting.

The invention belongs to the technical field of old vinegar brewing and particularly relates to a vinegar and a preparation method thereof. The method includes raw material preprocessing, smashing, moisturizing, pasting, liquefying, liquid stationary alcoholic fermentation, stirring, vat feeding, solid acetic acid fermentation, smoking, vinegar pouring and ageing. Meanwhile, a fulled-sealed device is adopted for automatic stirring, producing and fermenting during alcohol fermentation, a traditional vat is adopted during acetic acid fermentation, fermentation is implemented through manual turning, and the finished vinegar can be obtained. The method has the advantages of strain excellence and process reasonableness, the fermentation period is shortened, the raw material utilization rate and vinegar finishing rate are increased, season limitation is eliminated, and the advantages of sour, sweet, soft and sweet tastes of the traditional vinegar are maintained.

The invention discloses a rhizobium capable of efficiently solubilizing phosphorus and application of the rhizobium. The phosphorus solubilizing rhizobium is named as Rhizobium sp.P-1 and preserved in China Center For Type Culture Collection (CCTCC) on June 16, 2016, and the preservation number is CCTCC M 2016333. The rhizobium can convert difficultly-soluble inorganic phorphorus into a high-quality phosphorus compound which can be directly absorbed and utilized by plants. By using the screened rhizobium in a lettuce pot culture experiment, the content of effective phosphorus in soil can be obviously increased, it is guaranteed that the lettuce yield is not decreased on the condition that the application amount of phosphate fertilizer is decreased by 50%, and the nutritional quality of lettuce is improved. The rhizobium can be used for preparing a phosphorus solubilizing fungicide, be widely applied to agricultural production, improve the bio-availability of difficultly-soluble phosphorus in the soil, increase the phosphate fertilizer utilization efficiency, save fertilizer and increase the yield and has the important significance and application value on the aspect of keeping agricultural ecological balance.

The invention relates to brewing yeast engineering bacteria containing Lg-ATF1 genes; a method for preparing the yeast engineering bacteria provided by The invention comprises the following steps: selecting strong promoters PGK1 so as to over express specific Lg-ATF1 genes coding alcohol acetyl transferase of beer yeast in brewing yeast, thereby obtaining the yeast engineering bacteria SaccharomycescerevisiaeEY-15 containing Lg-ATF1 genes, wherein a preservation number is CGMCC (China General Microbiological Culture Collection Center) No.5635. According to The invention, on a condition that other fermenting performance is not influenced, transformant bacterial strains are compared with parent strains (SaccharomycescerevisiaeCGMCC No.1525); after simulation of semi-solid fermentation, the content of ethyl acetate is increased by 1.6 times; the content of isoamyl acetate is increased to 26.6mg / L; the sifted engineering bacteria has no specific demand for fermentation equipment and conditions; the equipment and conditions of normal white sprits factories can be used; therefore, the engineering bacteria has wide application propose and brings marked economic benefit to industrial production of the white sprits factories.

The invention discloses Saccharomyces cerevisiae genetic engineering bacteria with high ester yield. The Saccharomyces cerevisiae genetic engineering bacteria EY-13 were collected in China General Microbiological Culture Collection Center on November 17, 2010, the collection number is CGMCC NO.4350, and the Saccharomyces cerevisiae genetic engineering bacteria are recommended to be named Saccharomyces cerevisiae. PGK1 derived from the Saccharomyces cerevisiae is selected as a promoter; and the construction method of the Saccharomyces cerevisiae genetic engineering bacteria with the high ester yield comprises a step of knocking-out an IAH1 gene for encoding ester hydrolase in a Saccharomyces cerevisiae genome when an ATF1 gene which is derived from the Saccharomyces cerevisiae and is used for encoding alcohol acetyltransferase is overexpressed. Compared with initial recipient bacteria, the Saccharomyces cerevisiae genetic engineering bacteria have the advantages that: after rice wine fermentation is simulated, the content of isoamylol is about one half, the content of ethyl acetate is improved by 20 times, the content of isoamyl acetate is 100mg / L, and the content of isobutyl acetate is 5 to 7mg / L; and after liquor fermentation is simulated, the content of total esters is improved by 4 times and the content of the ethyl acetate is improved by 35 times, so an excellent strain is provided for the production of brewing industry.

The invention relates to a method for preparing a complex enzyme protein, which is characterized by comprising the following steps: preparing an aerobic fermentation medium, preparing an anaerobic fermentation medium, sterilizing the mediums, activating bacterial strains, performing amplification cultivation and inoculation, preparing an aerobic fermentation condition, preparing an anaerobic fermentation condition, drying at a low temperature, mixing the mixture, and packaging. The method has the advantages that: 1, the strains are excellent and the passage stability is strong; 2, each strain plays a synergetic role in the process of fermentation, the growth and the metabolism of each strain is thriving, and the enzyme productivity is strong; 3, aerobic zymogens and anaerobic zymogens perform fermentations separately so that each strain gives play to the best enzyme producing performance; and 4, proteins which are difficult to absorb by animals in raw materials are converted into thalli single cell proteins which are easy to absorb so that the nutritive value of products is improved.

The invention discloses a construction method of a saccharomyces cerevisiae strain with a high yield of isobutanol and relates to preparation of microorganism by a recombinant DNA (Deoxyribonucleic Acid) technology. According to the method, the saccharomyces cerevisiae strain with the high yield of isobutanol is constructed by reforming saccharomyces cerevisiae cells by using molecular biology and the gene recombination technology. The method comprises the following steps: S1, constructing plasmids of over-expressing ILV2 (Induced Leukemia Virus), ILV3, ILV5 and ZWF1 genes of saccharomyces cerevisiae; and S2, constructing a saccharomyces cerevisiae strain HBGDAL-103 with the high yield of isobutanol. The method disclosed by the invention overcomes the deficiency that further improvement of the yield of isobutanol is restricted by factors such as imbalance of a coenzyme NADPH / NADP<+> (Reduced Form of Nicotinamide-Adenine Dinucleotide Phosphate) / (Nicotinamide-Adenine Dinucleotide Phosphate<+>) from pyruvic acid to 2-one isovaleric acid due to over-expressed ILV2, ILV3 and ILV5 in the saccharomyces cerevisiae cells of yielding isobutanol in the prior art.

The invention discloses a strain of Aspergillus niger ( Aspergillus niger ) engineering strain, the Aspergillus niger engineering strain is to knock out the fumarate hydratase gene fum of Aspergillus niger engineered strains. The invention overcomes the deficiencies in the prior art. In the existing process of using Aspergillus niger to ferment and produce malic acid, the by-product fumaric acid will accumulate along with the production of malic acid, resulting in an increase in the cost of the subsequent malic acid purification process. The present invention provides a fum Knockout engineered strains of Aspergillus niger, and a method for greatly reducing fumaric acid by-products during fermentation by Aspergillus niger. The invention significantly reduces the by-product fumaric acid accumulated in the process of producing malic acid by fermentation of Aspergillus niger, reduces the cost in the process of downstream separation and purification of malic acid, and provides excellent strains for industrial fermentation and production of malic acid.

The invention discloses a method for improving a competitive nodulation capacity of nodule bacteria USDA110. A dgoK1 singly inserted mutant strain USDA110 (dgoKmut) is constructed through pK19mob, under the soil potting condition, compared with a plant inoculated with USDA110, the fresh weight, nodule number, nodule weight and enzyme activity of a plant inoculated with USDA110 (dgoKmut) are all significantly increased; compared with indigenous nodule bacteria, the competitive nodulation capacity of the USDA110 (dgoKmut) is improved by 24.5%, and can reach 31.8%. Therefore, the USDA110 (dgoKmut) has good symbiotic nitrogen fixation and competitive nodulation capacities, and can be used as a promising strain in field experiments.

The invention belongs to the field of edible mushrooms and discloses a novel pholiota nameko mushroom block, a mushroom block production process and a special device of production of the mushroom block. The pholiota nameko mushroom block comprises a strain, a cultivation material and a mushroom bag with a breathable film; the production process of the mushroom block comprises the steps of bagging, pressing blocks, sterilizing, inoculating, culturing; the production of the mushroom block is realized by a special device which is block pressing machine; the mushroom block and the production process of the mushroom block are low in pollution rate; through an automatic production device, the production efficiency is improved and the production cost is reduced; meanwhile, the yearly culture times are increased; a factory is positioned in an artificial climate chamber, mycelia are cultured; the mushroom blocks capable of directly producing mushrooms are delivered to mushroom growers; after the mushroom growers cultivate the mushrooms, the mushrooms are changed from being harvested for one time in a year to being harvested for 2-4 times in a year.

The invention provides a Lactobacillus plantarum strain with nitrite degrading activity and ACE inhibitory activity and application thereof, which has degrading effect on nitrite and can be widely used in fermented food and fermented feed. The preservation number of Lactobacillus sp. FJS 002 provided by the present invention is CGMCC No 18378. The plant lactobacillus provided by the invention is applied in the field of food processing or feed processing. The present invention obtains excellent bacterial strains with efficient degradative effect on nitrite and inhibitory effect on ACE by screening, and can provide theoretical basis and scientific knowledge for solving the problem of nitrite residue in food, especially fermented food, and the high blood pressure that plagues human health. means.

The invention discloses a high-salt-tolerance Zygosaccharomyces rouxii A strain of which the collection number is CCTCC NO:M2013310. The strain has high salt tolerance and favorable growth stability, has the characteristic of being capable of producing full-bodied mellow and ester-like aromatic flavors, and can be used for brewing high-salt diluted soy sauce, shortening the fermentation period and improving the flavor and quality of the soy sauce.

The present invention relates to a genetically engineered bacterial strain of Aspergillus niger with high organic acid production under low dissolved oxygen conditions. The construction steps of the genetically engineered bacterial strain are as follows: step 1, constructing a heterologously expressed vhb gene plasmid; the gene vhb sequence The fragment is controlled by the Aspergillus niger 3-glycerol phosphate dehydrogenase gene promoter PgpdA; step 2, the acquisition of the heterologous expression vhb gene strain, namely the Aspergillus niger (Aspergillus niger) genetically engineered strain with high organic acid production under low dissolved oxygen conditions. The present invention is based on the natural characteristics of Aspergillus niger to produce organic acids, and through genetic recombination to transform the physiological characteristics of Aspergillus niger, a genetically engineered strain of Aspergillus niger is obtained. Experiments have confirmed that the genetically engineered strain of Aspergillus niger can produce organic acids under low dissolved oxygen conditions. The acid ability is significantly improved, which provides excellent strains for the preparation of organic acids by microbial fermentation.

The invention discloses Pseudomonas nitroreducens and application thereof, belonging to the field of environmental microorganism. The Pseudomonas nitroreducens CQ1 of the invention is derived from the sewage of oxidation ponds in the sewage treatment system of Beijing Yanshan Petrochemical Co., Ltd., and obtained by enriching, separating and purifying. The Pseudomonas nitroreducens CQ1 is cultured and collected in the China General Microbiological Culture Collection Center (hereinafter referred to as CGMCC) with the collection number being CGMCC No.3159. The invention further discloses the application of Pseudomonas nitroreducens in the biodegradation of quinoline or derivatives thereof. Quinoline or derivatives thereof with the concentration reaching 700mg / L can be degraded by Pseudomonas nitroreducens of the invention, and the degradability can reach 100% after treating for 72h. Moreover, the Pseudomonas nitroreducens CQ1 of the invention is sensitive to kanamycin sulfate, tetracycline and gentamicin and does not carry plasmids.

The invention relates to high-yield acetate brewing yeast engineering bacteria; a method for preparing the brewing yeast bacteria provided by The invention comprises the following steps: selecting strong promoters PGK1 so as to overexpress ATF2 gene coding alcohol acetyl transferase, thereby obtaining high-yield acetate brewing yeast engineering bacteria SaccharomycescerevisiaeEY-14, wherein a preservation number is CGMCC (China General Microbiological Culture Collection Center) No.5635. According to The invention, on a condition that other fermenting performance is not influenced, transformant bacterial strains are compared with parent strains (SaccharomycescerevisiaeCGMCC No 2.1525); after simulation of semi-solid fermentation, the content of ethyl acetate is increased by 3.6 times; the content of isoamyl acetate is increased to 55.2mg / L; the content of isobutyl acetate is increased to 33.8mg / L; the sifted engineering bacteria has no specific demand for fermentation equipment and conditions; the equipment and conditions of normal white sprits factories can be used; therefore, the engineering bacteria has wide application propose and brings marked economic benefit to industrial production of the white sprits factories.

The invention discloses a high-efficiency phosphorus-dissolving rhizobia and its application. The phosphorus-dissolving rhizobia is named Rhizobium Rhizobium sp.P‑1 has been deposited in the China Center for Type Culture Collection (CCTCC) on June 16, 2016, with the deposit number CCTCC M 2016333. The rhizobia can convert insoluble inorganic phosphorus into high-quality phosphorus compounds that can be directly absorbed and utilized by plants. In the lettuce pot test, the rhizobia screened by the present invention can significantly increase the content of available phosphorus in the soil, and in the case of reducing the amount of phosphorus fertilizer applied by 50%, the lettuce output is not reduced, and the nutritional quality of the lettuce is also improved. . The rhizobia can be used to prepare phosphate-dissolving bacteria, which is widely used in agricultural production to improve the bioavailability of insoluble phosphorus in soil and the utilization efficiency of phosphate fertilizers, reduce fertilizer and increase production, and has important significance and application value in maintaining agricultural ecological balance. .

The invention provides a Bacillus subtilis used for inhibiting Phellodendron twig blight, the preservation number is ATCC NO.6051, and the bacterial strain has good control effect on Phellodendron twig blight.

The invention discloses the application of Bacillus subtilis subsp.natto ACE-IP and Bacillus subtilis subsp.natto ACE-IP in the preparation of ACE-inhibiting peptides from fermented scallop skirts, and its preservation number is CCTCC NO: M 2018081. The screening and identification method of Bacillus subtilis natto subspecies ACE‑IP includes: raw material processing and amino acid composition analysis; preparation of scallop skirt fermentation medium; primary screening of Bacillus natto; secondary screening of Bacillus natto; identification of strains . The Bacillus natto screened by the present invention is prepared by fermenting the scallop skirt to obtain the ACE inhibitory peptide, which fully exerts the effect of producing various proteases during the fermentation of the scallop skirt, and decomposes the scallop skirt macromolecular protein into small molecule ACE Inhibiting peptides with high inhibitory activity; not only provide excellent strains for industrial production of ACE inhibitory peptides that are safe, have no side effects and have high activity, but also provide a theoretical basis for the development, utilization and further processing of scallop skirts.