Preparation method of composite zymoprotein

A composite enzyme and protein technology, applied in the field of composite enzyme protein preparation, can solve the problems of poor stability, low activity and high production cost, and achieve the effects of improved nutritional value, enhanced enzyme-producing ability, and excellent strains

Inactive Publication Date: 2009-08-19
江西普润药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the early days, amylase was obtained from barley malt, and later pepsin and trypsin from animal sources were used; papain (papain) from plant sources; phytase was produced from Aspergillus, but in a long historical period, due to production Due to problems such as high cost, low activity, and poor stability, it has not been able to enter the practical stage.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Aerobic fermentation medium configuration: mix blood meal, leather powder, feather and bran in a weight ratio of 1:2:1:12, and mix the mixture with water in a weight ratio of 1.8:1;

[0019] 2. Anaerobic fermentation medium configuration: mix pomace, rice bran, and sweet potato powder residue in a weight ratio of 3:1:1, and mix the mixture with water in a weight ratio of 6:4;

[0020] 3. Medium sterilization: Put the medium into the sterilizer for high-temperature steam sterilization;

[0021] 4. Strain activation: ① Configure the basic medium according to the aerobic fermentation strain and the anaerobic fermentation strain; ② Packing: divide the prepared medium into the triangular flask to be inoculated, and plug it with a cotton plug; ③ Sterilization: Sterilize at 0.1MPa for 15 minutes, then let it naturally depressurize to normal pressure, cool, and wait for inoculation; ④ Inoculation: Wait for the medium to cool to 40°C for inoculation, and the inoculum volume i...

Embodiment 2

[0030] 1. Aerobic fermentation medium configuration: mix blood meal, leather powder, feather and bran at a ratio of 1:2:1:12, and mix the mixture with water at a ratio of 1.8:1;

[0031] 2. Anaerobic fermentation medium configuration: mix pomace, rice bran, and sweet potato powder residue at a ratio of 3:1:1, and mix the mixture with water at a ratio of 6:4;

[0032] 3. Medium sterilization: Put the medium into the sterilizer for high-temperature steam sterilization;

[0033] 4. Strain activation: ① Configure the basic medium according to the requirements of each strain; ② Packing: divide the prepared medium into the triangular flask to be inoculated, and plug it with a cotton plug; ③ Sterilization: at 0.1MPa Sterilize for 15 minutes, then let it naturally reduce the pressure to normal pressure, cool down, and wait for inoculation; ④ Inoculation: Wait for the medium to cool to 40°C to inoculate, and the inoculation amount is 0.5%; ⑤ Culture: Put the inoculated test tube into a...

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PUM

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Abstract

The invention relates to a method for preparing a complex enzyme protein, which is characterized by comprising the following steps: preparing an aerobic fermentation medium, preparing an anaerobic fermentation medium, sterilizing the mediums, activating bacterial strains, performing amplification cultivation and inoculation, preparing an aerobic fermentation condition, preparing an anaerobic fermentation condition, drying at a low temperature, mixing the mixture, and packaging. The method has the advantages that: 1, the strains are excellent and the passage stability is strong; 2, each strain plays a synergetic role in the process of fermentation, the growth and the metabolism of each strain is thriving, and the enzyme productivity is strong; 3, aerobic zymogens and anaerobic zymogens perform fermentations separately so that each strain gives play to the best enzyme producing performance; and 4, proteins which are difficult to absorb by animals in raw materials are converted into thalli single cell proteins which are easy to absorb so that the nutritive value of products is improved.

Description

technical field [0001] The invention relates to a method for preparing an enzyme protein, in particular to a method for preparing a complex enzyme protein. Background technique [0002] The idea and practice of adding "enzyme" to feed to improve the nutritional value of feed and the production performance of livestock and poultry has a history of decades. As early as the 1950s, Jensen et al. (1957) and Fry et al. (1958) reported that the addition of crude amylase and protease could significantly increase the growth rate and feed conversion rate of chickens. In the early days, amylase was obtained from barley malt, and later pepsin and trypsin from animal sources were used; papain (papain) from plant sources; phytase was produced from Aspergillus, but in a long historical period, due to production Due to problems such as high cost, low activity, and poor stability, it has not been able to enter the practical stage. Around the 1990s, due to the rapid development of bioengine...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16A23K1/165A23K20/189
Inventor 杨涛
Owner 江西普润药业有限公司
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