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A kind of high-efficiency phosphorus-solubilizing rhizobia and its application

A rhizobia and phosphorus-dissolving technology, applied in the field of microorganisms, can solve the problem that the phosphorus-dissolving mechanism of bacteria is not completely clear

Active Publication Date: 2019-05-10
武汉一禾生态环境检测中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there are always some problems, such as the phosphorus-solubilizing mechanism of the bacteria is not completely clear, which needs to be further explored, and more importantly, there are not many high-efficiency phosphorus-solubilizing rhizobia with practical application value.

Method used

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  • A kind of high-efficiency phosphorus-solubilizing rhizobia and its application
  • A kind of high-efficiency phosphorus-solubilizing rhizobia and its application
  • A kind of high-efficiency phosphorus-solubilizing rhizobia and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Obtaining of P1:

[0033] The medium formula involved in this embodiment is as follows:

[0034] The plate screening medium is: glucose 10g, tricalcium phosphate 5g, ammonium sulfate 0.5g, sodium chloride 0.3g, potassium chloride 0.3g, magnesium sulfate heptahydrate 0.3g, ferrous sulfate heptahydrate 0.03g, manganese sulfate monohydrate 0.03g, 18g agar powder, 1L distilled water, pH 7.0-7.2, sterilize at 121°C for 20 minutes, ready for use.

[0035] Shake flask re-screening medium is: glucose 10g, tricalcium phosphate 5g, magnesium chloride 5g, magnesium sulfate heptahydrate 0.25g, potassium chloride 0.2g, ammonium sulfate 0.1g, distilled water 1L, pH7.0-7.2, quenched at 121℃ Bacteria for 20 minutes, cool and store at 30-40℃ for later use.

[0036] LB liquid medium: tryptone 10g, yeast extract powder 5g, NaCl 10g, distilled water 1L, pH7.0-7.2, sterilize at 121℃ for 20 minutes, cool and store at 30-40℃ for later use.

[0037] Preliminary screening of phosphate-dissolving bacter...

Embodiment 2

[0044] Identification of Phosphate Solubilizing Bacteria P1

[0045] 1) The morphological characteristics of the phosphate solubilizing bacteria P1 colony:

[0046] After culturing on LB medium at 28-32°C for 2-3 days, the colony of P1 strain was round, dot-shaped, with neat edges, moist and smooth surface, opaque, white, and center bulge.

[0047] 2) Physiological and biochemical characteristics of phosphate solubilizing bacteria P1:

[0048] Phosphorus solubilizing bacteria P1 is a trophic, gram-negative bacteria; it cannot use citrate and propionate, and can grow normally under 2% NaCl medium, but cannot grow on medium higher than 5% NaCl; contact with enzymes Reaction, VP reaction, oxidase reaction, glucose fermentation acid production, H2S production, nitrate reduction, exercise test are positive; methyl red test, gelatin liquefaction and starch hydrolysis test are negative; glucose, arabinose and wood can be used Sugar grows vigorously, and mannose cannot be used.

[0049] 3) Mo...

Embodiment 3

[0053] The fermentation method of Rhizobium sp.P-1 includes:

[0054] 1) Preparation of seed medium

[0055] Tryptone 10g, yeast extract 5g, NaCl 10g, 1L water, pH 7.2-7.5, sterilize at 121°C for 20-40 minutes, and cool to 30-40°C for later use;

[0056] 2) Preparation of fermentation medium

[0057] Fermentation medium components are: glucose 1.5%, soybean meal 1%, corn meal 0.5%, peptone 0.5%, dipotassium hydrogen phosphate 0.05%, magnesium sulfate 0.03%, manganese sulfate 0.002%, ferrous sulfate 0.002%, all of the above For mass ratio, pH 8.0, sterilize at 121°C for 20 minutes, cool and store at 30-40°C for later use;

[0058] 3) Seed expansion cultivation

[0059] Take 1-2 loops of the lawn of Rhizobium P1 from the slope, inoculate it into 50ml of sterilized seed culture medium, place it on a shaker at 30°C and rotate at 200r / min for 22h, and use it as a fermentation seed solution for use;

[0060] 4) Preparation of Rhizobium P1

[0061] Put the fermentation seed liquid prepared in st...

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Abstract

The invention discloses a high-efficiency phosphorus-dissolving rhizobia and its application. The phosphorus-dissolving rhizobia is named Rhizobium Rhizobium sp.P‑1 has been deposited in the China Center for Type Culture Collection (CCTCC) on June 16, 2016, with the deposit number CCTCC M 2016333. The rhizobia can convert insoluble inorganic phosphorus into high-quality phosphorus compounds that can be directly absorbed and utilized by plants. In the lettuce pot test, the rhizobia screened by the present invention can significantly increase the content of available phosphorus in the soil, and in the case of reducing the amount of phosphorus fertilizer applied by 50%, the lettuce output is not reduced, and the nutritional quality of the lettuce is also improved. . The rhizobia can be used to prepare phosphate-dissolving bacteria, which is widely used in agricultural production to improve the bioavailability of insoluble phosphorus in soil and the utilization efficiency of phosphate fertilizers, reduce fertilizer and increase production, and has important significance and application value in maintaining agricultural ecological balance. .

Description

Technical field [0001] The invention belongs to the technical field of microorganisms, and specifically relates to a rhizobia with high efficiency in dissolving phosphorus and its application. Background technique [0002] Phosphorus is one of the three essential nutrient elements for plant growth. The total phosphorus content of soil in my country is relatively high. The total phosphorus content of cultivated soil is generally 0.02%-0.11%, but the effective phosphorus content that can be directly absorbed and utilized by plants generally does not exceed total phosphorus. 5% of the amount. Most phosphate fertilizer and Ca in soil 2+ , Fe 3+ , Fe 2+ , Al 3+ In our country, 74% of arable land is deficient in phosphorus, and more than 95% of the phosphorus in the soil is in ineffective form. The lack of available phosphorus in the soil is one of the main factors restricting plant growth. In order to obtain high yields, people repeatedly apply a large amount of phosphate fertilizer t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08C12R1/41
CPCC05F11/08C12N1/20C12N1/205C12R2001/41
Inventor 杜雷陈钢张利红洪娟黄翔王素萍练志诚叶莉霞张贵友
Owner 武汉一禾生态环境检测中心
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