Rhizobium capable of efficiently solubilizing phosphorus and application of rhizobium

A rhizobia and phosphorus-resolving technology, applied in the field of microorganisms, can solve the problem that the phosphorus-resolving mechanism of bacteria is not completely clear.

Active Publication Date: 2016-09-21
武汉一禾生态环境检测中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there are always some problems, such as the phosphorus-solubilizing mechanism of the bacteria is not completely clear, which needs to be further explored, and more importantly, there are not many high-efficiency phosphorus-solubilizing rhizobia with practical application value.

Method used

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  • Rhizobium capable of efficiently solubilizing phosphorus and application of rhizobium
  • Rhizobium capable of efficiently solubilizing phosphorus and application of rhizobium
  • Rhizobium capable of efficiently solubilizing phosphorus and application of rhizobium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Obtaining Phosphobacteria P1:

[0033] The culture medium formula involved in this embodiment is as follows:

[0034] Plate screening medium is: glucose 10g, tricalcium phosphate 5g, ammonium sulfate 0.5g, sodium chloride 0.3g, potassium chloride 0.3g, magnesium sulfate heptahydrate 0.3g, ferrous sulfate heptahydrate 0.03g, manganese sulfate monohydrate 0.03g, 18g agar powder, 1L distilled water, pH7.0-7.2, sterilized at 121°C for 20 minutes, set aside.

[0035]Shake flask re-screening medium is: glucose 10g, tricalcium phosphate 5g, magnesium chloride 5g, magnesium sulfate heptahydrate 0.25g, potassium chloride 0.2g, ammonium sulfate 0.1g, distilled water 1L, pH7.0-7.2, sterilized at 121°C Bacteria for 20 minutes, cool at 30-40°C for later use.

[0036] LB liquid medium: tryptone 10g, yeast extract powder 5g, NaCl 10g, distilled water 1L, pH7.0-7.2, sterilized at 121°C for 20 minutes, cooled at 30-40°C for later use.

[0037] Primary screening of phosphate-solubiliz...

Embodiment 2

[0044] Identification of Phosphobacteria P1

[0045] 1) Colony morphological characteristics of Phosphobacteria P1:

[0046] After cultured on LB medium at 28-32°C for 2-3 days, the colonies of the P1 strain were round, dot-shaped, with neat edges, moist and smooth surface, opaque, white, and raised in the center.

[0047] 2) Physiological and biochemical characteristics of Phosphobacteria P1:

[0048] Phosphorus solubilizer P1 is a trophic, Gram-negative bacterium; it cannot utilize citrate and propionate, it can grow normally on a medium lower than 2% NaCl, and cannot grow on a medium higher than 5% NaCl; contact with enzymes Reaction, V-P reaction, oxidase reaction, glucose fermentation acid production, H2S production, nitrate reduction, motility test were positive; methyl red test, gelatin liquefaction and starch hydrolysis test were negative; glucose, arabinose and xylose could be utilized Sugar thrives and cannot utilize mannose.

[0049] 3) Molecular biological ident...

Embodiment 3

[0053] The fermentation method of Rhizobium sp.P-1, comprising:

[0054] 1) Preparation of seed medium

[0055] Tryptone 10g, yeast extract 5g, NaCl 10g, water 1L, pH 7.2-7.5, sterilize at 121°C for 20-40 minutes, cool at 30-40°C for later use;

[0056] 2) Preparation of fermentation medium

[0057] The components of the fermentation medium are: glucose 1.5%, soybean flour 1%, corn flour 0.5%, peptone 0.5%, dipotassium hydrogen phosphate 0.05%, magnesium sulfate 0.03%, manganese sulfate 0.002%, ferrous sulfate 0.002%, all of the above is the mass ratio, pH 8.0, sterilized at 121°C for 20 minutes, cooled at 30-40°C for later use;

[0058] 3) Seed expansion and cultivation

[0059] Take the lawn of 1-2 rings of Rhizobium P1 from the slope, inoculate it into 50ml of sterilized seed culture medium, place it on a shaker at 30°C with a rotation speed of 200r / min for 22h, and use it as a fermented seed solution for later use;

[0060] 4) Preparation of Rhizobium P1

[0061] Put ...

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Abstract

The invention discloses a rhizobium capable of efficiently solubilizing phosphorus and application of the rhizobium. The phosphorus solubilizing rhizobium is named as Rhizobium sp.P-1 and preserved in China Center For Type Culture Collection (CCTCC) on June 16, 2016, and the preservation number is CCTCC M 2016333. The rhizobium can convert difficultly-soluble inorganic phorphorus into a high-quality phosphorus compound which can be directly absorbed and utilized by plants. By using the screened rhizobium in a lettuce pot culture experiment, the content of effective phosphorus in soil can be obviously increased, it is guaranteed that the lettuce yield is not decreased on the condition that the application amount of phosphate fertilizer is decreased by 50%, and the nutritional quality of lettuce is improved. The rhizobium can be used for preparing a phosphorus solubilizing fungicide, be widely applied to agricultural production, improve the bio-availability of difficultly-soluble phosphorus in the soil, increase the phosphate fertilizer utilization efficiency, save fertilizer and increase the yield and has the important significance and application value on the aspect of keeping agricultural ecological balance.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a high-efficiency phosphorus-dissolving rhizobia and application thereof. Background technique [0002] Phosphorus is one of the three essential nutrients for plant growth. The total phosphorus content of soil in my country is relatively high, and the total phosphorus content of cultivated soil is generally 0.02%-0.11%, but the effective phosphorus content that can be directly absorbed and utilized by plants generally does not exceed total phosphorus. 5% of the amount. Most of the phosphorus fertilizers and Ca in the soil 2+ , Fe 3+ , Fe 2+ 、Al 3+ etc. combined to form insoluble phosphate. In my country, 74% of the cultivated land is deficient in phosphorus, and more than 95% of the phosphorus in the soil is in an invalid form. The lack of available phosphorus in the soil is one of the main factors that limit plant growth. In order to obtain high yield, peo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08C12R1/41
CPCC05F11/08C12N1/20C12N1/205C12R2001/41
Inventor 杜雷陈钢张利红洪娟黄翔王素萍练志诚叶莉霞张贵友
Owner 武汉一禾生态环境检测中心
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