42 results about "Indophenol" patented technology

The present invention describes methods and kits for determining the concentration of free chlorine in water. To avoid false readings from other species present in the water, the free chlorine is reacted with ammonia to form monochloramine, which is then reacted to form an indophenol or an indonaphthol. The concentration of the indophenol or indonaphthol is proportional to the total monochloramine present. Subtracting the concentration of residual monochloramine from the total monochloramine yields the concentration of monochloramine formed from the free chlorine and ammonia, and is proportional to the concentration of free chlorine. Embodiments of the present invention will simplify and accelerate accurate measurements of free chlorine in water, without interference from compounds impacting present measurements of free chlorine.

The invention provides a degradation bacterium which can degrade butyl 2, 4-dichlorophenoxy acetate pesticide residue and a microbial inoculum produced by the degradation bacterium, pertaining to the high biotechnology field, wherein, the applied strains are Gram-stain negative bacteria Ld1 which are identified as Pseudomonas sp.; the degradation bacterium is mainly and biologically characterizedin that: the main biological characteristic is G<->; thallus takes the shape of short rods, and the size of the thallus is about 0.5 Mum to 1.0 Mum multiplied by 1.5 Mum to 5.0 Mum; oxidase, catalase, nitrate reduction and citrate utilization reactions are positive; lipase reactions, V.P. reactions, MR reactions, indophenol reactions are negative; and starch can not be hydrolyzed. The microbial inoculum is obtained after the completion of the fermentation of the Ld1 strains. The application of the degradation bacterium products can reduce the residual quantity of butyl 2, 4-dichlorophenoxy acetate pesticides by more than 70 percent and can solve the problem that butyl 2, 4-dichlorophenoxy acetate pesticide residue in the agricultural production exceeds the standard.

The invention relates to a mycotoxin degrading bacterial strain and a method for preparing the preparation of the same, and is characterized in that the accession number of the bacterial strain is CCTCC M 208087; the bacterium is short-rod shaped, non spore forming, gram-negative, ivory when cultured in colony in a TSA culture medium and aerobic; catalase-positive, oxidase-negative,urease-positiveand indophenol reaction negative, capable of hydrolysising esculin, incapable of dual hydrolysising Arginine, casein and starch, incapable of decomposing tyrosine and liquefying glutin, capable of using glucose, incapable of using L-arabinose, D-mannose and maltose; the accession number of the strain 16S rDNA in Genbank is EU794908. The invention also provides the method for preparing liquid microbial inoculums, solid microbial inoculums and mycotoxin absorbents with the strain.

The invention belongs to the field of crop pesticide residue detection and biochemical detection, and discloses an automatic portable paper-based micro-fluidic photoelectric detection device and method of pesticide residue. A paper-based chip carrying an indophenol acetate reagent in a first test paper box is conveyed by a chip replacing and conveying belt to a chip installing strip, a paper-based chip carrying an acetylcholin esterase reagent in a second test paper box is conveyed by a chip outgoing and conveying belt to a lower installing strip of an acetylcholin esterase paper-based micro-fluidic chip; a reagent enters a sampling tank through a sampling port, enters an acetylcholin esterase fixed groove under the effect of a paper-based chip sampling channel and is mixed with the acetylcholin esterase; the chip installing strip is driven to move downwards by pressing a component, so that the indophenol acetate reagent and the acetylcholin esterase reagent are in full contact with each other and generate color reaction, and a color region is detected by a photoelectric detection device. The automatic portable paper-based micro-fluidic photoelectric detection device and method are based on an enzyme inhibition reaction principle, obviously improve the detection resolution and sensitivity, and realize an automatic process from inputting a sample to be detected to displaying the amount of pesticide residue.

The invention provides a method for determining ammonium nitrogen, nitrate nitrogen, nitrite nitrogen and total dissolved nitrogen in a sample, and belongs to the technical field of nitrogen content determination. When the ammonium nitrogen is measured, an oxidizing agent (sodium hypochlorite and sodium chloro isocyanurate) and color developing agent (salicylic acid) system is adopted, the ammonium nitrogen is oxidized into monochloramine under the action of the oxidizing agent, the formed monochloramine and the color developing agent form indophenol, and measurement is carried out by measuring the ultraviolet absorbance at the position of 660 nm; when nitrate nitrogen, nitrite nitrogen and total dissolved nitrogen are measured, a new first Griess reagent (N-(1-naphthyl) ethylenediamine dihydrochloride) and a second Griess reagent system (sulfanilamide and vanadium chloride) are adopted for measurement, the detection limit is low, and the sensitivity is high. The chromogenic reaction is stable and uniform in speed and can be carried out on a 96-well plate.

A spectrophotometric method for the determination of pyruvate dehydrogenase activity. This method uses a spectrophotometer, in the presence of substrate sodium pyruvate and cofactors magnesium chloride, thiamine pyrophosphate, using phenazine dimethyl sulfate as electron transporter, thiazole blue as electron acceptor, thiazole blue Reducted by pyruvate dehydrogenase (E1) product hydroxyethyl-pyrophosphate thiamine, the maximum absorption peak of thiazole blue changes from wavelength 400-430 nanometers to 540-640 nanometers, by measuring the wavelength 540-640 nanometers light absorption The amount of increase determines the reduction of thiazolium blue and defines the activity of the enzyme. The reagents used in this method are more economical, more stable, and more sensitive than the 2,6-dichloroindoxyl method and the potassium ferricyanide method. The influence of reagents is not conducive to the determination of E1 activity in the pyruvate dehydrogenase complex. This method is not only suitable for the determination of purified pyruvate dehydrogenation activity, but also for pyruvate dehydrogenation in the pyruvate dehydrogenase complex after mitochondria extraction. Hydrogenase activity assay.