42 results about "Indophenol" patented technology

The present invention describes methods and kits for determining the concentration of free chlorine in water. To avoid false readings from other species present in the water, the free chlorine is reacted with ammonia to form monochloramine, which is then reacted to form an indophenol or an indonaphthol. The concentration of the indophenol or indonaphthol is proportional to the total monochloramine present. Subtracting the concentration of residual monochloramine from the total monochloramine yields the concentration of monochloramine formed from the free chlorine and ammonia, and is proportional to the concentration of free chlorine. Embodiments of the present invention will simplify and accelerate accurate measurements of free chlorine in water, without interference from compounds impacting present measurements of free chlorine.

The invention relates to a mycotoxin degrading bacterial strain and a method for preparing the preparation of the same, and is characterized in that the accession number of the bacterial strain is CCTCC M 208087; the bacterium is short-rod shaped, non spore forming, gram-negative, ivory when cultured in colony in a TSA culture medium and aerobic; catalase-positive, oxidase-negative,urease-positive and indophenol reaction negative, capable of hydrolysising esculin, incapable of dual hydrolysising Arginine, casein and starch, incapable of decomposing tyrosine and liquefying glutin, capable of using glucose, incapable of using L-arabinose, D-mannose and maltose; the accession number of the strain 16S rDNA in Genbank is EU794908. The invention also provides the method for preparing liquid microbial inoculums, solid microbial inoculums and mycotoxin absorbents with the strain.

An extraction method and applications of acetylcholinesterase relate to a method to extract acetylcholinesterase from carp and a method to detect the residue of organic phosphorous pesticides. The extraction method is that: the tissues of carp are undergone homogenate by phosphorous acid buffer solution in ice bath, and is froze and centrifuging, and then the enzyme liquid is prepared. The detecting method is that: acetylcholinesterase of carp brain is used for an enzyme source, 2, 6-dichloro Indophenol Sodium salt is used for a color developing agent, qualitative filter paper is used for a vector, and the enzyme is immobilized by absorption. The detailed operation is that: a plastized hard piece of paper is stuck with small pieces of paper; one side of the hard paper is added with the color developing agent and acetylthiocholine iodide, the other side acetylcholinesterase solution, and the hard paper is put into a frigirater to be froze and dried and can be fetched when needed; the eluant of organic phosphorous pesticide is dripped on and fully wet two filter papers, and the two pieces of filter paper are overlapped for minutes to observe if the color is changed; the color of the filter paper changes from blue to colorless if organic pesticide is not existed, otherwise the blue color does not fade or does not obviously fade, the higher the concentration is, the more obviously the color change is. The invention has the advantages of low cost, rapid detecting speed and high sensibility.

The invention relates to quick test paper for organic phosphorus and carbamic acid ester type pesticide residues. The technical scheme of the quick test paper is as follows: the test paper is made by the temperature-sensitive microcapsule technology; and encapsulated cholinesterase is released in a special temperature clinical region to catalyze indophenol acetate to hydrolyze and develop color; therefore, the pesticide residue condition is determined. The quick test paper is disposable and low in price; a reagent is not needed to prepare and the maintenance work of the analytic instrument is omitted; the test paper is simpler than manual pressing in use and the result determination is clear at a glance without training; the dosage of the reagent is low, the detection time is short and the detection can be completed in 1 minutes; the cholinesterase is stable under the protection of microcapsules and long in storage time; the thermal environment in the range from 37 to 40 DEG C of solution is easy to realize, and the temperature is quite convenient and accurate to control, and insufficient moisture and too low temperature situations can not occur; besides, the quick test paper is convenient for popularization, and is the first choice for places such as farm product market, supermarket, school, hotel, factory and the like to quickly detect pesticide residues.

This invention relates to a culture medium for the rapid detection of coliform and coliform groups, which is mixed from distilled water solution containing peptone, yeast extract, lactose, 4-methylumbelliferone-beta-D-galactoside, sodium chloride, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, sodium lauryl sulfate and agar (or agar-free), the solution of indophenol-beta-D-glucoside and solution of cefsulodin. Peptone, yeast extract, lactose, 4-methylumbelliferone-beta-D-galactoside, sodium chloride, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, sodium lauryl sulfate and agar (or agar-free) are weighed according to a certain criterion and added to distilled water for heat dissolution. The consequent solution is adjusted to a certain pH value, put into a high pressure sterilizer for sterilization and then added with filtrated and sterilized indophenol-beta-D-glucoside solution and cefsulodin solution after the temperature lowered to 50 deg.Cí‚5 deg.C. This is useful in either qualitative or quantitative detection of coliform and coliform groups in drinking water, environmental water and foods, high efficiency and accurate detection result.

The present disclosure relates to a bio-sensor capable of measuring the total concentration of one or a plurality of ammonia or ammonium ions with the use of indophenol reagents in the presence of an ionomer. In some embodiments, the biosensor comprises a perflurinated membrane that comprises an ionomer in contact with an alkaline buffer in a vessel configured to receive a sample, such as whole blood. The disclosure also relates to a method of detecting or quantifying the ammonia or ammonium ion concentration in whole blood in a point of care biosensor without reliance on gas chromatography or any measurement that takes more than about twenty minutes.

The invention relates to the field of a biological detection instrument, and particularly relates to a biosensor based on glucose dehydrogenase. The biosensor is composed of an insulation substrate, an electrode system, a detecting reagent, a middle interlayer, a covering layer, a reaction room and a sample injection channel; the reaction room is provided with air holes. Enzyme in the detection reagent is flavin adenine dinucleotide dependent form glucose dehydrogenase; the electronic medium is commonly formed by ruthenium complex and 2, 6-dichlorophen indophenol. The invention further provides a detecting method of the biosensor. The biosensor solves the problem that the pure ruthenium complex is very hard to accept electronic from the flavin adenine dinucleotide dependent form glucose dehydrogenase, thus the biosensor has the advantages of good reaction sensitivity and linearity.

The invention discloses a reagent for detecting helicobacter pylori infection in gastric juice and a detection method thereof and belongs to the technical field of enzyme analysis. According to the prepared reagent, viscous materials in the gastric juice are decomposed into a soluble liquid by virtue of ambroxol hydrochloride, so that the helicobacter pylori in the gastric juice is dissociated and is added into a filter, the filter is taken down from a reaction device, free helicobacter pylori flows into a reaction hole along with a pretreatment fluid, a phenol reagent and an alkaline sodium hypochlorite solution are added, the helicobacter pylori has a urease strengthening effect, the urea is decomposed to generate dimolecular ammonia and monomolecular carbon dioxide after the helicobacter pylori is reacted with urea on an acetate fiber membrane, the ammonia is reacted with phenol and hypochlorous acid in an alkaline medium so as to generate blue indophenol, and the content of the blue indophenol is in direct proportion to the content of the helicobacter pylori. The detection method disclosed by the invention is simple in pretreatment, rapid, sensitive, accurate and low in price, is suitable for detecting the helicobacter pylori in the gastric juice and can serve as an important check index of detection of the helicobacter pylori in the gastric juice.

The invention discloses a synthesis method of glucoside based on an indoxyl derivative and a 2-(benzothiazol-2'-yl)phenol derivative. The general formula of a synthetic equation of the synthesis method is as shown in the description; the Ar-OH represents aromatic phenol or a ketonic compound thereof, and is used as a glycosyl receptor in a glycosylation reaction; the G-X represents halogenated sugar, and is used as a glycosyl donor in the glycosylation reaction. According to the synthesis method of the glucoside based on the indoxyl derivative and the 2-(benzothiazol-2'-yl)phenol derivative, a 1-acetylindolin-3-one derivative is selected and used as an intermediate; a novel synthesis method is researched and developed to prepare the glucoside based on the indoxyl derivative; a novel glycosidase fluorescence probe based on a BTP ((2-benzothiazol-2'-yl)phenyl) derivative is synthesized by applying the method; meanwhile, the application effects of the glucoside based on the indoxyl derivative and the glycosidase fluorescence probe are investigated. The glucoside based on the indoxyl derivative and the 2-(benzothiazol-2'-yl)phenol derivative can be used as glycosidase substrates, and is used for the positioning analytic detection and screening on the corresponding glycosidase, a microorganism using the corresponding glycosidase as a characteristic target, and the like.

The invention provides a detecting method for pesticide residue in fruits. The method comprises the following steps that a fruit sample is selected, and smashing and homogenating are carried out; after the smashing and the homogenating are carried out, the fruit sample is added into deionized water to be subjected to soaking, hydroquinone and sodium thiosulfate are added, and homogenizing is carried out; after the homogenizing is carried out, a solution is subjected to centrifuging, and sediments are removed; cholinesterase and 2,6-dichloro indophenol acetate are added into obtained supernatant. The detecting method is simple in processing and operating, high in extracting efficiency, capable of avoiding heavy metal ions and the reaction temperature interfere a detecting result, and widely applied to detecting the pesticide residue in the various fruits.

The invention provides a test strip capable of detecting organophosphorus and carbamate pesticide residues in edible agricultural products. The test strip is an agricultural residue detection test strip composed of a substrate sheet with the diameter of 11 mm to 20 mm, cholinesterase with the viability unit of 40 to 75 U, and liquid indophenol acetate with absorbance of 0.2 at 490 nm. In detection, a sample is pretreated, a drop of the pretreated solution of the sample is added into the test strip and undergoes a reaction at 37 DEG C for 6 min, the color development result is observed, and ifthe color is blue, there are not pesticide residues, if the color is white, there are organophosphorus and carbamate pesticide residues. The pesticide residue card manufacturing process and the detection process do not need expensive instruments and reagents, are easy to operate and have high sensitivity.

The invention discloses a preparation method for synthesizing indigotin through continuous oxidation, which comprises the following steps: introducing ammonia gas into liquid sodium metal, and performing reaction in an ammonia-sodium synthesizing tower to generate sodium amide; adding the sodium amide into alkali according to certain proportion, and adding the mixture into a material-mixing reactor continuously or at intervals; at certain temperature and pressure, using the alkali as a solvent and the sodium amide as a condensating agent, and adding anilino-acetate continuously to cyclize the anilino-acetate into hydroxy indole phenolate; discharging the materials continuously and diluting the materials with water, and then adding 3 to 20 percent w/w of indole phenolate solution into an oxidation reaction tower continuously from the upper part of the tower; introducing air into the tower from the lower part of the tower at a temperature of between 60 and 80 DEG C; after the continuous cyclic oxidations by an oxidation tower and oxidation reaction devices, sending the materials oxidized to the end point to post-processing from the bottom of the tower; and obtaining the indigotin through filtering, washing and drying. The preparation method improves the reaction yield and the product quality, shortens the reaction period, and greatly reduces the energy consumption.

The invention belongs to the technical field of nitrogen content determination, and provides a determination method for ammonium nitrogen, nitric nitrogen, nitrite nitrogen and total dissolved nitrogen in samples. The method provided by the invention has the advantages that during determination of the ammonium nitrogen, a new oxidant (sodium hypochlorite and sodium chlorinated isocyanurate) and color developer (salicylic acid) system is adopted, the ammonium nitrogen is oxidized into monochloramine under the action of an oxidant, indophenol is obtained from the obtained monochloramine and a color developer, and the ultraviolet absorbance at a wavelength of 660nm is determined for measurement; a new first Griess reagent (N-(1-naphthyl)ethylenediamine dihydrochloride) and second Griess reagent (sulfanilamide and vanadium chloride) system is adopted for measurement of the nitric nitrogen, the nitrite nitrogen and the total dissolved nitrogen, so that low detection limits and high sensitivity are achieved; and the color development reaction is stable in speed and uniform, so as to be carried out on a 96-well plate.

The invention relates to a preparation method of an indophenol derivative, which is prepared by adding sodium carbonate and sodium persulfate into acetylamino-carrying phenol and N, N-dialkyl-carrying alkylaniline hydrochloride to make them undergo an oxidation reaction. The method can alleviate environmental pollution, increase reaction yield, and effectively overcome the defects in an indophenol derivative traditional preparation process.

The invention relates to an addition type silicone rubber impression material, and belongs to the field of medical dental materials. The addition type silicone rubber impression material comprises the following raw materials by mass: 25-35 parts of vinyl polydimethyl siloxane, 1-10 parts of hydrosilicone oil, 1-5 parts of a catalyst, 3-10 parts of an enhancer, 55-65 parts of a compatibilizer, 0.1-3 parts of a hydrophilic modifier, 1-2 parts of a non-ionic surfactant, 0.1-0.5 part of indophenol, 0.1-1 part of benzoic acid alkyl ester, 0.1-1 part of a pigment, and 0.1-1.5 parts of peppermint oil. The addition type silicone rubber impression material comprises the non-ionic surfactant, namely nonylphenol polyoxyethylene ether, the hydrophobicity can be improved, and besides, the hydrophilic modifier can also enable all of the components to be uniformly dispersed and mixed, so that the impression is more accurate, and the detail reproduction is better.

The present disclosure relates to hair-coloring compositions comprising direct dyes, and aerosol hair-coloring products comprising the hair-coloring compositions. The hair-coloring compositions include: (a) at least one direct dye selected from nitro-phenylenediamines, nitro-aminophenols, azo dyes, anthraquinones, triarylmethane dyes, indophenols, and a mixture thereof; (b) at least one nonionic film forming polymer; (c) at least one thickening agent; (d) at least 40 wt. % of water, based on the total weight of the compositions; and (e) 40 wt. % or less of ethanol, based on the total weight of the composition. The hair-coloring compositions can be combined with a propellant and incorporated into a canister to provide a hair-coloring aerosol product. The compositions and aerosol products are useful for coloring hair.

The invention provides a matched artemisia apiacea and agastache rugosaus combination medicine, a method for preparing isatin through fresh folium isatidis as the raw material and the application of the matched artemisia apiacea and agastache rugosaus combination medicine. The matched artemisia apiacea and agastache rugosaus combination medicine is characterized in that isatin is added to fresh folium isatidis; after indoxyl is generated, oxygen is supplied to the indoxyl through dissolved oxygen in original soak solution, therefore, through the control of the pH of the system and the control of oxygen in the enzymolysis process, a reaction is carried out in the direction of forming of the isatin; the method is easy, convenient to adopt and controllable; because the fresh folium isatidis directly serves as the reaction material, production cost is effectively reduced; because further oxidization and generation of indoxyl free radicals are controlled to prevent isatin from being generated again and the isatin is directly added to the reaction raw materials, forward-direction balancing of the reaction is achieved.

The present disclosure relates to a biosensor capable of measuring the total concentration of one or a plurality of ammonia or ammonium ions with the use of indophenol reagents in the presence of an ionomer. In some embodiments, the biosensor comprises a perflurinated membrane that comprises an ionomer in contact with an alkali buffer in a vessel configured to receive a sample, such as whole blood. The disclosure also relates to a method of detecting or quantifying the ammonia or ammonium ion concentration in whole blood in a point of care bio sensor without reliance on gas chromatography or any measurement that takes more than about twenty minutes.

The invention provides a nutritional cream for pet dogs with renal failure and a preparation method of the nutritional cream, and relates to the technical field of animal feeding. The nutritional cream for pet dogs with renal failure provided by the present invention is composed of the following raw materials in parts by weight: 10-20 parts of gluten meal, 10-30 parts of inulin, 2-5 parts of beta-hydroxy-beta-calcium methyl butyrate, 20-30 parts of zeolite, 2-4 parts of leucine, 30-50 parts of egg white, 50-70 parts of chicken, 50-70 parts of lotus root starch, 10-20 parts of corn germ oil, 50-80 parts of corn flour, and 0.3-1 part of preservative. The preparation method is to prepare a homogenate, conduct pulverizing, filtering, and stirring into a paste. According to the present invention, uremic toxins such as urea nitrogen, creatinine, inorganic phosphorus, indophenol sulfate, etc., can be reduced, the symptoms of renal failure can be alleviated, residual renal function can be protected, the disease condition can be stabilized, and the life quality of CRF pet dogs can be improved.