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42 results about "Indophenol" patented technology
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Indophenol is an organic compound with the formula OC₆H₄NC₆H₄OH. It is deep blue dye that is the product of the Berthelot's reaction, a common test for ammonia. The indophenol group, with various substituents in place of OH and various ring substitutions, is found in many dyes used in hair coloring and textiles.
Detection of free chlorine in water
InactiveUS20090320570A1Prevents and reduces over-estimationProblematic interferenceWater treatment parameter controlChemical analysis using titrationAmmoniaIndophenol
The present invention describes methods and kits for determining the concentration of free chlorine in water. To avoid false readings from other species present in the water, the free chlorine is reacted with ammonia to form monochloramine, which is then reacted to form an indophenol or an indonaphthol. The concentration of the indophenol or indonaphthol is proportional to the total monochloramine present. Subtracting the concentration of residual monochloramine from the total monochloramine yields the concentration of monochloramine formed from the free chlorine and ammonia, and is proportional to the concentration of free chlorine. Embodiments of the present invention will simplify and accelerate accurate measurements of free chlorine in water, without interference from compounds impacting present measurements of free chlorine.
Owner:HACH CO
Automatic portable paper-based micro-fluidic photoelectric detection device and method of pesticide residue
InactiveCN106680518AReduce processing timeLow costMaterial analysis by observing effect on chemical indicatorPesticide residueEnzyme inhibition
The invention belongs to the field of crop pesticide residue detection and biochemical detection, and discloses an automatic portable paper-based micro-fluidic photoelectric detection device and method of pesticide residue. A paper-based chip carrying an indophenol acetate reagent in a first test paper box is conveyed by a chip replacing and conveying belt to a chip installing strip, a paper-based chip carrying an acetylcholin esterase reagent in a second test paper box is conveyed by a chip outgoing and conveying belt to a lower installing strip of an acetylcholin esterase paper-based micro-fluidic chip; a reagent enters a sampling tank through a sampling port, enters an acetylcholin esterase fixed groove under the effect of a paper-based chip sampling channel and is mixed with the acetylcholin esterase; the chip installing strip is driven to move downwards by pressing a component, so that the indophenol acetate reagent and the acetylcholin esterase reagent are in full contact with each other and generate color reaction, and a color region is detected by a photoelectric detection device. The automatic portable paper-based micro-fluidic photoelectric detection device and method are based on an enzyme inhibition reaction principle, obviously improve the detection resolution and sensitivity, and realize an automatic process from inputting a sample to be detected to displaying the amount of pesticide residue.
Owner:JIANGSU UNIV
Bacterial strain capable of degrading mold toxin and formulation preparation method thereof
The invention relates to a mycotoxin degrading bacterial strain and a method for preparing the preparation of the same, and is characterized in that the accession number of the bacterial strain is CCTCC M 208087; the bacterium is short-rod shaped, non spore forming, gram-negative, ivory when cultured in colony in a TSA culture medium and aerobic; catalase-positive, oxidase-negative,urease-positive and indophenol reaction negative, capable of hydrolysising esculin, incapable of dual hydrolysising Arginine, casein and starch, incapable of decomposing tyrosine and liquefying glutin, capable of using glucose, incapable of using L-arabinose, D-mannose and maltose; the accession number of the strain 16S rDNA in Genbank is EU794908. The invention also provides the method for preparing liquid microbial inoculums, solid microbial inoculums and mycotoxin absorbents with the strain.
Owner:JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
Extraction method for acetylcholinesterase and application thereof
InactiveCN101126082ALow costQuick checkHydrolasesMicrobiological testing/measurementPhosphorous acidColor changes
An extraction method and applications of acetylcholinesterase relate to a method to extract acetylcholinesterase from carp and a method to detect the residue of organic phosphorous pesticides. The extraction method is that: the tissues of carp are undergone homogenate by phosphorous acid buffer solution in ice bath, and is froze and centrifuging, and then the enzyme liquid is prepared. The detecting method is that: acetylcholinesterase of carp brain is used for an enzyme source, 2, 6-dichloro Indophenol Sodium salt is used for a color developing agent, qualitative filter paper is used for a vector, and the enzyme is immobilized by absorption. The detailed operation is that: a plastized hard piece of paper is stuck with small pieces of paper; one side of the hard paper is added with the color developing agent and acetylthiocholine iodide, the other side acetylcholinesterase solution, and the hard paper is put into a frigirater to be froze and dried and can be fetched when needed; the eluant of organic phosphorous pesticide is dripped on and fully wet two filter papers, and the two pieces of filter paper are overlapped for minutes to observe if the color is changed; the color of the filter paper changes from blue to colorless if organic pesticide is not existed, otherwise the blue color does not fade or does not obviously fade, the higher the concentration is, the more obviously the color change is. The invention has the advantages of low cost, rapid detecting speed and high sensibility.
Owner:HUAZHONG AGRI UNIV
Quick test paper for organic phosphorus and carbamic acid ester type pesticide residues
InactiveCN103163131AReduce dosageShort detection timeMaterial analysis by observing effect on chemical indicatorColor/spectral properties measurementsPesticide residueMoisture
The invention relates to quick test paper for organic phosphorus and carbamic acid ester type pesticide residues. The technical scheme of the quick test paper is as follows: the test paper is made by the temperature-sensitive microcapsule technology; and encapsulated cholinesterase is released in a special temperature clinical region to catalyze indophenol acetate to hydrolyze and develop color; therefore, the pesticide residue condition is determined. The quick test paper is disposable and low in price; a reagent is not needed to prepare and the maintenance work of the analytic instrument is omitted; the test paper is simpler than manual pressing in use and the result determination is clear at a glance without training; the dosage of the reagent is low, the detection time is short and the detection can be completed in 1 minutes; the cholinesterase is stable under the protection of microcapsules and long in storage time; the thermal environment in the range from 37 to 40 DEG C of solution is easy to realize, and the temperature is quite convenient and accurate to control, and insufficient moisture and too low temperature situations can not occur; besides, the quick test paper is convenient for popularization, and is the first choice for places such as farm product market, supermarket, school, hotel, factory and the like to quickly detect pesticide residues.
Owner:QINGDAO UNIV
Rapid detection card for detecting pesticide residues, preparation method and application thereof
InactiveCN102399850AHigh activityImprove stabilityMicrobiological testing/measurementPesticide residueAcetylcholinesterase
The invention relates to a rapid detection card for detecting pesticide residues, a preparation method and application thereof. The fast detection card comprises a coverage film, a first plastic board, sample loading holes, an enzyme tablet, a plastic film, a second plastic board and a substrate sheet, wherein the coverage film is adhered to the surface of the first plastic board; at least two sample loading holes are formed in the first plastic board; the enzyme tablet fixed with housefly acetylcholinesterase is adhered to the reverse sides of the sample loading holes and is opposite to the sample loading holes; the substrate sheet, which is fixed with acetyl indophenol and is adhered to the position corresponding to the enzyme tablet, is arranged on the second plastic board; and the plastic film is arranged in the middles of the first plastic board and the second plastic board. In the rapid detection card, enzyme is the housefly acetylcholinesterase which is subjected to molecular evolution, so the rapid detection card has the advantages of high activity, high sensitivity, high stability and the like and can be applied to the rapid detection of organophosphorus and carbamic acid ester pesticide residues in agricultural products, food and soil and particularly vegetables, fruits.
Owner:SHANGHAI ACAD OF AGRI SCI
Quick detecting coliform group and medium for culturing coliforms and preparation method
InactiveCN1796568AShorten the timeImprove efficiencyMicrobiological testing/measurementEscherichia coliDipotassium hydrogen phosphate
This invention relates to a culture medium for the rapid detection of coliform and coliform groups, which is mixed from distilled water solution containing peptone, yeast extract, lactose, 4-methylumbelliferone-beta-D-galactoside, sodium chloride, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, sodium lauryl sulfate and agar (or agar-free), the solution of indophenol-beta-D-glucoside and solution of cefsulodin. Peptone, yeast extract, lactose, 4-methylumbelliferone-beta-D-galactoside, sodium chloride, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, sodium lauryl sulfate and agar (or agar-free) are weighed according to a certain criterion and added to distilled water for heat dissolution. The consequent solution is adjusted to a certain pH value, put into a high pressure sterilizer for sterilization and then added with filtrated and sterilized indophenol-beta-D-glucoside solution and cefsulodin solution after the temperature lowered to 50 deg.Cí‚5 deg.C. This is useful in either qualitative or quantitative detection of coliform and coliform groups in drinking water, environmental water and foods, high efficiency and accurate detection result.
Owner:CHONGQING FOOD IND RES INST
Device And Methods Of Using Device For Detection Of Hyperammonemia
ActiveUS20160231310A1Spectrum investigationMaterial analysis by observing effect on chemical indicatorIonomerHyperammonemia
The present disclosure relates to a bio-sensor capable of measuring the total concentration of one or a plurality of ammonia or ammonium ions with the use of indophenol reagents in the presence of an ionomer. In some embodiments, the biosensor comprises a perflurinated membrane that comprises an ionomer in contact with an alkaline buffer in a vessel configured to receive a sample, such as whole blood. The disclosure also relates to a method of detecting or quantifying the ammonia or ammonium ion concentration in whole blood in a point of care biosensor without reliance on gas chromatography or any measurement that takes more than about twenty minutes.
Owner:UNIV OF MARYLAND +2
Biosensor based on glucose dehydrogenase and detecting method
InactiveCN107064261ASolving the difficulty of accepting electrons from flavin adenine dinucleotide (FAD)-dependent glucose dehydrogenaseGood response sensitivityMaterial analysis by electric/magnetic meansFlavin adenine dinucleotideDichlorophenolindophenol
The invention relates to the field of a biological detection instrument, and particularly relates to a biosensor based on glucose dehydrogenase. The biosensor is composed of an insulation substrate, an electrode system, a detecting reagent, a middle interlayer, a covering layer, a reaction room and a sample injection channel; the reaction room is provided with air holes. Enzyme in the detection reagent is flavin adenine dinucleotide dependent form glucose dehydrogenase; the electronic medium is commonly formed by ruthenium complex and 2, 6-dichlorophen indophenol. The invention further provides a detecting method of the biosensor. The biosensor solves the problem that the pure ruthenium complex is very hard to accept electronic from the flavin adenine dinucleotide dependent form glucose dehydrogenase, thus the biosensor has the advantages of good reaction sensitivity and linearity.
Owner:TAIZHOU E LINKCARE MEDITECH CO LTD
Detection of free chlorine in water
InactiveUS8065906B2Prevents and reduces over-estimationWater treatment parameter controlChemical analysis using titrationAmmoniaNH3 compound
The present invention describes methods and kits for determining the concentration of free chlorine in water. To avoid false readings from other species present in the water, the free chlorine is reacted with ammonia to form monochloramine, which is then reacted to form an indophenol or an indonaphthol. The concentration of the indophenol or indonaphthol is proportional to the total monochloramine present. Subtracting the concentration of residual monochloramine from the total monochloramine yields the concentration of monochloramine formed from the free chlorine and ammonia, and is proportional to the concentration of free chlorine. Embodiments of the present invention will simplify and accelerate accurate measurements of free chlorine in water, without interference from compounds impacting present measurements of free chlorine.
Owner:HACH CO
Method for detecting helicobacter pylori infection in gastric juice
InactiveCN104561241ALow viscosityEasy to cough upMicrobiological testing/measurementMicroorganism based processesFiberPhenol
The invention discloses a reagent for detecting helicobacter pylori infection in gastric juice and a detection method thereof and belongs to the technical field of enzyme analysis. According to the prepared reagent, viscous materials in the gastric juice are decomposed into a soluble liquid by virtue of ambroxol hydrochloride, so that the helicobacter pylori in the gastric juice is dissociated and is added into a filter, the filter is taken down from a reaction device, free helicobacter pylori flows into a reaction hole along with a pretreatment fluid, a phenol reagent and an alkaline sodium hypochlorite solution are added, the helicobacter pylori has a urease strengthening effect, the urea is decomposed to generate dimolecular ammonia and monomolecular carbon dioxide after the helicobacter pylori is reacted with urea on an acetate fiber membrane, the ammonia is reacted with phenol and hypochlorous acid in an alkaline medium so as to generate blue indophenol, and the content of the blue indophenol is in direct proportion to the content of the helicobacter pylori. The detection method disclosed by the invention is simple in pretreatment, rapid, sensitive, accurate and low in price, is suitable for detecting the helicobacter pylori in the gastric juice and can serve as an important check index of detection of the helicobacter pylori in the gastric juice.
Owner:TIANJIN BAODI HOSPITAL
Synthesis method of glucoside based on indoxyl derivative and 2-(benzothiazol-2'-yl)phenol derivative
ActiveCN106432369AEasy to operateHigh yieldSugar derivativesFluorescence/phosphorescenceMicroorganismIndoxyl
The invention discloses a synthesis method of glucoside based on an indoxyl derivative and a 2-(benzothiazol-2'-yl)phenol derivative. The general formula of a synthetic equation of the synthesis method is as shown in the description; the Ar-OH represents aromatic phenol or a ketonic compound thereof, and is used as a glycosyl receptor in a glycosylation reaction; the G-X represents halogenated sugar, and is used as a glycosyl donor in the glycosylation reaction. According to the synthesis method of the glucoside based on the indoxyl derivative and the 2-(benzothiazol-2'-yl)phenol derivative, a 1-acetylindolin-3-one derivative is selected and used as an intermediate; a novel synthesis method is researched and developed to prepare the glucoside based on the indoxyl derivative; a novel glycosidase fluorescence probe based on a BTP ((2-benzothiazol-2'-yl)phenyl) derivative is synthesized by applying the method; meanwhile, the application effects of the glucoside based on the indoxyl derivative and the glycosidase fluorescence probe are investigated. The glucoside based on the indoxyl derivative and the 2-(benzothiazol-2'-yl)phenol derivative can be used as glycosidase substrates, and is used for the positioning analytic detection and screening on the corresponding glycosidase, a microorganism using the corresponding glycosidase as a characteristic target, and the like.
Owner:GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1
Detecting method for pesticide residue in fruits
InactiveCN104964868AEasy to operateAvoid interferenceMaterial analysis by observing effect on chemical indicatorPreparing sample for investigationCholinesterasePesticide residue
The invention provides a detecting method for pesticide residue in fruits. The method comprises the following steps that a fruit sample is selected, and smashing and homogenating are carried out; after the smashing and the homogenating are carried out, the fruit sample is added into deionized water to be subjected to soaking, hydroquinone and sodium thiosulfate are added, and homogenizing is carried out; after the homogenizing is carried out, a solution is subjected to centrifuging, and sediments are removed; cholinesterase and 2,6-dichloro indophenol acetate are added into obtained supernatant. The detecting method is simple in processing and operating, high in extracting efficiency, capable of avoiding heavy metal ions and the reaction temperature interfere a detecting result, and widely applied to detecting the pesticide residue in the various fruits.
Owner:DONGCHEN LEADER TESTING
2,4-dichlorobenzene oxygen butyl acetate pesticide residue degradation bacterium and produced bacterium agent thereof
InactiveCN101402927AWill not affect the use effectEasy to useBacteriaMicroorganism based processesPesticide residueOxygen
The invention provides a degradation bacterium which can degrade butyl 2, 4-dichlorophenoxy acetate pesticide residue and a microbial inoculum produced by the degradation bacterium, pertaining to the high biotechnology field, wherein, the applied strains are Gram-stain negative bacteria Ld1 which are identified as Pseudomonas sp.; the degradation bacterium is mainly and biologically characterized in that: the main biological characteristic is G<->; thallus takes the shape of short rods, and the size of the thallus is about 0.5 Mum to 1.0 Mum multiplied by 1.5 Mum to 5.0 Mum; oxidase, catalase, nitrate reduction and citrate utilization reactions are positive; lipase reactions, V.P. reactions, MR reactions, indophenol reactions are negative; and starch can not be hydrolyzed. The microbial inoculum is obtained after the completion of the fermentation of the Ld1 strains. The application of the degradation bacterium products can reduce the residual quantity of butyl 2, 4-dichlorophenoxy acetate pesticides by more than 70 percent and can solve the problem that butyl 2, 4-dichlorophenoxy acetate pesticide residue in the agricultural production exceeds the standard.
Owner:NANJING AGRICULTURAL UNIVERSITY
Test strip capable of detecting organophosphorus and carbamate pesticide residues in edible agricultural products through one-step method
InactiveCN108344732AEasy to readEasy to useMaterial analysis by observing effect on chemical indicatorCholinesterasePesticide residue
The invention provides a test strip capable of detecting organophosphorus and carbamate pesticide residues in edible agricultural products. The test strip is an agricultural residue detection test strip composed of a substrate sheet with the diameter of 11 mm to 20 mm, cholinesterase with the viability unit of 40 to 75 U, and liquid indophenol acetate with absorbance of 0.2 at 490 nm. In detection, a sample is pretreated, a drop of the pretreated solution of the sample is added into the test strip and undergoes a reaction at 37 DEG C for 6 min, the color development result is observed, and ifthe color is blue, there are not pesticide residues, if the color is white, there are organophosphorus and carbamate pesticide residues. The pesticide residue card manufacturing process and the detection process do not need expensive instruments and reagents, are easy to operate and have high sensitivity.
Owner:湖北致康生物科技有限公司
Preparation method for synthesizing indigotin through continuous oxidation
InactiveCN101591476ALower conversion rateImprove conversion rateBis-indole indigosAcetic acidLiquid metal
The invention discloses a preparation method for synthesizing indigotin through continuous oxidation, which comprises the following steps: introducing ammonia gas into liquid sodium metal, and performing reaction in an ammonia-sodium synthesizing tower to generate sodium amide; adding the sodium amide into alkali according to certain proportion, and adding the mixture into a material-mixing reactor continuously or at intervals; at certain temperature and pressure, using the alkali as a solvent and the sodium amide as a condensating agent, and adding anilino-acetate continuously to cyclize the anilino-acetate into hydroxy indole phenolate; discharging the materials continuously and diluting the materials with water, and then adding 3 to 20 percent w / w of indole phenolate solution into an oxidation reaction tower continuously from the upper part of the tower; introducing air into the tower from the lower part of the tower at a temperature of between 60 and 80 DEG C; after the continuous cyclic oxidations by an oxidation tower and oxidation reaction devices, sending the materials oxidized to the end point to post-processing from the bottom of the tower; and obtaining the indigotin through filtering, washing and drying. The preparation method improves the reaction yield and the product quality, shortens the reaction period, and greatly reduces the energy consumption.
Owner:JIANGSU TAIFENG CHEM
Determination method for ammonium nitrogen, nitric nitrogen, nitrite nitrogen and total dissolved nitrogen in samples
InactiveCN110346361AHigh precisionImprove accuracyMaterial analysis by observing effect on chemical indicatorColor/spectral properties measurementsETHYLENEDIAMINE DIHYDROCHLORIDESalicylic acid
The invention belongs to the technical field of nitrogen content determination, and provides a determination method for ammonium nitrogen, nitric nitrogen, nitrite nitrogen and total dissolved nitrogen in samples. The method provided by the invention has the advantages that during determination of the ammonium nitrogen, a new oxidant (sodium hypochlorite and sodium chlorinated isocyanurate) and color developer (salicylic acid) system is adopted, the ammonium nitrogen is oxidized into monochloramine under the action of an oxidant, indophenol is obtained from the obtained monochloramine and a color developer, and the ultraviolet absorbance at a wavelength of 660nm is determined for measurement; a new first Griess reagent (N-(1-naphthyl)ethylenediamine dihydrochloride) and second Griess reagent (sulfanilamide and vanadium chloride) system is adopted for measurement of the nitric nitrogen, the nitrite nitrogen and the total dissolved nitrogen, so that low detection limits and high sensitivity are achieved; and the color development reaction is stable in speed and uniform, so as to be carried out on a 96-well plate.
Owner:SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
Agricultural product pesticide residue detection method
InactiveCN109142322AQuick checkDetection speedMaterial analysis by observing effect on chemical indicatorAnalysis by thermal excitationPesticide residueHydrolysis
The invention discloses an agricultural product pesticide residue detection method. Detected samples are obtained, a small quantity of materials collected from all portions of a whole batch of analyzed materials become the detected samples, parts of the test samples are randomly drawn for analysis and test, and sampling units, addresses, dates, sampling conditions and sampling package conditions are detailedly written on the samples. By the aid of test paper with immobilized cholinesterase and indophenol acetate, catalysis, hydrolysis and discoloration processes are changed according to the inhibiting effect of organophosphorus pesticides on butyrylcholine esterase, so that whether the samples contain excessive organophosphorus pesticides or not can be judged, two batches of detection cards are simultaneously used for detection in the detection process, the probability of detection failure caused by enzyme failure can be reduced, detection liquid is burned, flame reaction is performed,organo-chlorine pesticides can be detected, detection speed is increased, detection time and steps are saved, detection range is widened, and the method has excellent popularization values.
Owner:滑县恒琢信息科技有限公司
2,4-dichlorobenzene oxygen butyl acetate pesticide residue degradation bacterium and produced bacterium agent thereof
InactiveCN101402927BEasy to useReduce production and use costsBacteriaMicroorganism based processesPesticide residueOxygen
The invention provides a degradation bacterium which can degrade butyl 2, 4-dichlorophenoxy acetate pesticide residue and a microbial inoculum produced by the degradation bacterium, pertaining to the high biotechnology field, wherein, the applied strains are Gram-stain negative bacteria Ld1 which are identified as Pseudomonas sp.; the degradation bacterium is mainly and biologically characterizedin that: the main biological characteristic is G<->; thallus takes the shape of short rods, and the size of the thallus is about 0.5 Mum to 1.0 Mum multiplied by 1.5 Mum to 5.0 Mum; oxidase, catalase, nitrate reduction and citrate utilization reactions are positive; lipase reactions, V.P. reactions, MR reactions, indophenol reactions are negative; and starch can not be hydrolyzed. The microbial inoculum is obtained after the completion of the fermentation of the Ld1 strains. The application of the degradation bacterium products can reduce the residual quantity of butyl 2, 4-dichlorophenoxy acetate pesticides by more than 70 percent and can solve the problem that butyl 2, 4-dichlorophenoxy acetate pesticide residue in the agricultural production exceeds the standard.
Owner:NANJING AGRICULTURAL UNIVERSITY
Preparation method of indophenol derivative
InactiveCN103319910AReduce pollutionEasy to operateIndamines/indophenolsImino compound preparationPhenolMedicinal chemistry
The invention relates to a preparation method of an indophenol derivative, which is prepared by adding sodium carbonate and sodium persulfate into acetylamino-carrying phenol and N, N-dialkyl-carrying alkylaniline hydrochloride to make them undergo an oxidation reaction. The method can alleviate environmental pollution, increase reaction yield, and effectively overcome the defects in an indophenol derivative traditional preparation process.
Owner:上海永鸿实业集团化学科技有限公司
Addition type silicone rubber impression material
InactiveCN104921958AMix and disperse evenlyImprove hydrophobicityImpression capsDental impression compositionsBenzoic acidPeppermints
The invention relates to an addition type silicone rubber impression material, and belongs to the field of medical dental materials. The addition type silicone rubber impression material comprises the following raw materials by mass: 25-35 parts of vinyl polydimethyl siloxane, 1-10 parts of hydrosilicone oil, 1-5 parts of a catalyst, 3-10 parts of an enhancer, 55-65 parts of a compatibilizer, 0.1-3 parts of a hydrophilic modifier, 1-2 parts of a non-ionic surfactant, 0.1-0.5 part of indophenol, 0.1-1 part of benzoic acid alkyl ester, 0.1-1 part of a pigment, and 0.1-1.5 parts of peppermint oil. The addition type silicone rubber impression material comprises the non-ionic surfactant, namely nonylphenol polyoxyethylene ether, the hydrophobicity can be improved, and besides, the hydrophilic modifier can also enable all of the components to be uniformly dispersed and mixed, so that the impression is more accurate, and the detail reproduction is better.
Owner:SUZHOU YOUJUN ENVIRONMENTAL SCI & TECH
Bacterial strain capable of degrading mold toxin and formulation preparation method thereof
The invention relates to a mycotoxin degrading bacterial strain and a method for preparing the preparation of the same, and is characterized in that the accession number of the bacterial strain is CCTCC M 208087; the bacterium is short-rod shaped, non spore forming, gram-negative, ivory when cultured in colony in a TSA culture medium and aerobic; catalase-positive, oxidase-negative,urease-positiveand indophenol reaction negative, capable of hydrolysising esculin, incapable of dual hydrolysising Arginine, casein and starch, incapable of decomposing tyrosine and liquefying glutin, capable of using glucose, incapable of using L-arabinose, D-mannose and maltose; the accession number of the strain 16S rDNA in Genbank is EU794908. The invention also provides the method for preparing liquid microbial inoculums, solid microbial inoculums and mycotoxin absorbents with the strain.
Owner:JIANGSU ACAD OF AGRI SCI
Hair-coloring compositions, aerosol products, and methods for coloring hair
ActiveUS20200129388A1Facilitate depositionIncrease intensityCosmetic preparationsHair cosmeticsAnthraquinonesPolymer science
The present disclosure relates to hair-coloring compositions comprising direct dyes, and aerosol hair-coloring products comprising the hair-coloring compositions. The hair-coloring compositions include: (a) at least one direct dye selected from nitro-phenylenediamines, nitro-aminophenols, azo dyes, anthraquinones, triarylmethane dyes, indophenols, and a mixture thereof; (b) at least one nonionic film forming polymer; (c) at least one thickening agent; (d) at least 40 wt. % of water, based on the total weight of the compositions; and (e) 40 wt. % or less of ethanol, based on the total weight of the composition. The hair-coloring compositions can be combined with a propellant and incorporated into a canister to provide a hair-coloring aerosol product. The compositions and aerosol products are useful for coloring hair.
Owner:LOREAL SA
Matched artemisia apiacea and agastache rugosaus combination medicine, preparation method and application thereof
InactiveCN103463185AActivity advantageProgressive coughRespiratory disorderDermatological disorderMedicineOxygen
The invention provides a matched artemisia apiacea and agastache rugosaus combination medicine, a method for preparing isatin through fresh folium isatidis as the raw material and the application of the matched artemisia apiacea and agastache rugosaus combination medicine. The matched artemisia apiacea and agastache rugosaus combination medicine is characterized in that isatin is added to fresh folium isatidis; after indoxyl is generated, oxygen is supplied to the indoxyl through dissolved oxygen in original soak solution, therefore, through the control of the pH of the system and the control of oxygen in the enzymolysis process, a reaction is carried out in the direction of forming of the isatin; the method is easy, convenient to adopt and controllable; because the fresh folium isatidis directly serves as the reaction material, production cost is effectively reduced; because further oxidization and generation of indoxyl free radicals are controlled to prevent isatin from being generated again and the isatin is directly added to the reaction raw materials, forward-direction balancing of the reaction is achieved.
Owner:CHONGQING ACAD OF CHINESE MATERIA MEDICA +3
An automatic and portable paper-based microfluidic pesticide residue photoelectric detection device and method
InactiveCN106680518BReduce processing timeLow costMaterial analysis by observing effect on chemical indicatorEnzyme inhibitionPesticide residue
Owner:JIANGSU UNIV
Device and methods of using device for detection of hyperammonemia
ActiveUS20180143210A1Organic active ingredientsMaterial analysis by observing effect on chemical indicatorPoint of careGas phase
The present disclosure relates to a biosensor capable of measuring the total concentration of one or a plurality of ammonia or ammonium ions with the use of indophenol reagents in the presence of an ionomer. In some embodiments, the biosensor comprises a perflurinated membrane that comprises an ionomer in contact with an alkali buffer in a vessel configured to receive a sample, such as whole blood. The disclosure also relates to a method of detecting or quantifying the ammonia or ammonium ion concentration in whole blood in a point of care bio sensor without reliance on gas chromatography or any measurement that takes more than about twenty minutes.
Owner:UNIV OF MARYLAND +1
Nutritional cream for pet dogs with renal failure and preparation method of nutritional cream
PendingCN110710600ALower pHPromote excretionAnimal feeding stuffAccessory food factorsDiseaseUrea nitrogen/Creatinine
The invention provides a nutritional cream for pet dogs with renal failure and a preparation method of the nutritional cream, and relates to the technical field of animal feeding. The nutritional cream for pet dogs with renal failure provided by the present invention is composed of the following raw materials in parts by weight: 10-20 parts of gluten meal, 10-30 parts of inulin, 2-5 parts of beta-hydroxy-beta-calcium methyl butyrate, 20-30 parts of zeolite, 2-4 parts of leucine, 30-50 parts of egg white, 50-70 parts of chicken, 50-70 parts of lotus root starch, 10-20 parts of corn germ oil, 50-80 parts of corn flour, and 0.3-1 part of preservative. The preparation method is to prepare a homogenate, conduct pulverizing, filtering, and stirring into a paste. According to the present invention, uremic toxins such as urea nitrogen, creatinine, inorganic phosphorus, indophenol sulfate, etc., can be reduced, the symptoms of renal failure can be alleviated, residual renal function can be protected, the disease condition can be stabilized, and the life quality of CRF pet dogs can be improved.
Owner:JIANGSU AGRI ANIMAL HUSBANDRY VOCATIONAL COLLEGE
Method for determining ammonium nitrogen, nitrate nitrogen, nitrite nitrogen and total dissolved nitrogen in sample
PendingCN114354594AHigh precisionImprove accuracyMaterial analysis by observing effect on chemical indicatorColor/spectral properties measurementsETHYLENEDIAMINE DIHYDROCHLORIDESulfanilamide
The invention provides a method for determining ammonium nitrogen, nitrate nitrogen, nitrite nitrogen and total dissolved nitrogen in a sample, and belongs to the technical field of nitrogen content determination. When the ammonium nitrogen is measured, an oxidizing agent (sodium hypochlorite and sodium chloro isocyanurate) and color developing agent (salicylic acid) system is adopted, the ammonium nitrogen is oxidized into monochloramine under the action of the oxidizing agent, the formed monochloramine and the color developing agent form indophenol, and measurement is carried out by measuring the ultraviolet absorbance at the position of 660 nm; when nitrate nitrogen, nitrite nitrogen and total dissolved nitrogen are measured, a new first Griess reagent (N-(1-naphthyl) ethylenediamine dihydrochloride) and a second Griess reagent system (sulfanilamide and vanadium chloride) are adopted for measurement, the detection limit is low, and the sensitivity is high. The chromogenic reaction is stable and uniform in speed and can be carried out on a 96-well plate.
Owner:SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
Device and methods of using device for detection of hyperammonemia
ActiveUS9952199B2Bioreactor/fermenter combinationsBiological substance pretreatmentsIonomerHyperammonemia
The present disclosure relates to a bio-sensor capable of measuring the total concentration of one or a plurality of ammonia or ammonium ions with the use of indophenol reagents in the presence of an ionomer. In some embodiments, the biosensor comprises a perflurinated membrane that comprises an ionomer in contact with an alkaline buffer in a vessel configured to receive a sample, such as whole blood. The disclosure also relates to a method of detecting or quantifying the ammonia or ammonium ion concentration in whole blood in a point of care biosensor without reliance on gas chromatography or any measurement that takes more than about twenty minutes.
Owner:UNIV OF MARYLAND +2
Spectrophotometry for testing activity of pyruvic acid dehydrogenase system
InactiveCN100523784CLow priceSmall standard errorMicrobiological testing/measurementColor/spectral properties measurementsThiamine pyrophosphatePotassium ferricyanide
A spectrophotometric method for the determination of pyruvate dehydrogenase activity. This method uses a spectrophotometer, in the presence of substrate sodium pyruvate and cofactors magnesium chloride, thiamine pyrophosphate, using phenazine dimethyl sulfate as electron transporter, thiazole blue as electron acceptor, thiazole blue Reducted by pyruvate dehydrogenase (E1) product hydroxyethyl-pyrophosphate thiamine, the maximum absorption peak of thiazole blue changes from wavelength 400-430 nanometers to 540-640 nanometers, by measuring the wavelength 540-640 nanometers light absorption The amount of increase determines the reduction of thiazolium blue and defines the activity of the enzyme. The reagents used in this method are more economical, more stable, and more sensitive than the 2,6-dichloroindoxyl method and the potassium ferricyanide method. The influence of reagents is not conducive to the determination of E1 activity in the pyruvate dehydrogenase complex. This method is not only suitable for the determination of purified pyruvate dehydrogenation activity, but also for pyruvate dehydrogenation in the pyruvate dehydrogenase complex after mitochondria extraction. Hydrogenase activity assay.
Owner:HUAZHONG NORMAL UNIV
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