3 results about "Indophenol" patented technology

This invention relates to the field of medical device testing technology, specifically to the application of LC-MS / MS in determining the content of indophenol sulfate and p-cresol sulfate in a hemoperfusion device. The method includes: mixing plasma and acetonitrile, vortexing and centrifuging, collecting the supernatant, eluting the supernatant through a C18 solid-phase extraction column, collecting the eluent, mixing the eluent with water and filtering to obtain the test solution; performing liquid chromatography and mass spectrometry on the test solution, wherein the mobile phase A of the liquid chromatography is a 3-8 mmol / L gradient concentration ammonium acetate solution containing 0.05% ammonium formate by volume, and the mobile phase B is methanol; S3: calculating the concentrations of indophenol sulfate and p-cresol sulfate in the test solution based on the labeled curve. This invention, through dual purification pretreatment and multi-ion-pair threshold matching technology, can effectively remove more than 95% of interfering protein-binding toxoids. The interference peak response value in blank plasma is less than 5% of the target analyte response value, solving the detection error problem caused by interference in traditional methods.

This invention relates to a method for detecting indophenol sulfate based on surface-enhanced Raman spectroscopy. A liquid containing the indophenol sulfate to be detected is mixed with an oxidizing reagent to specifically oxidize the indophenol sulfate to indigo, yielding a mixed solution. This mixed solution is then mixed with gold nanoparticles, and the spectra of the mixed solution are acquired using a confocal Raman microscope. The acquired Raman spectra are baseline-removed to obtain the signal intensity at the target characteristic peak. Based on standard curves for different concentrations of indophenol sulfate and the signal intensity of the sample, the concentration of indophenol sulfate in the test sample is determined. This invention provides clear detection signals, accurate resolution, and a simple operation, enabling rapid and accurate detection of indophenol sulfate concentration in samples (such as serum). It is expected to promote research on the biological mechanisms of indophenol sulfate and its large-scale clinical application.

This invention relates to the field of indophenol sulfate and p-cresol sulfate technology, and particularly to a method for determining the concentrations of indophenol sulfate and p-cresol sulfate in plasma. The method involves pretreating the sample, followed by determination of the concentrations of indophenol sulfate and p-cresol sulfate in the sample using liquid chromatography-mass spectrometry and an internal standard method. The sample includes plasma, and the pretreatment involves protein precipitation. This method exhibits good linearity, a low limit of quantitation, strong anti-interference capabilities, and high precision and accuracy.