2,4-dichlorobenzene oxygen butyl acetate pesticide residue degradation bacterium and produced bacterium agent thereof

Abstract

The invention provides a degradation bacterium which can degrade butyl 2, 4-dichlorophenoxy acetate pesticide residue and a microbial inoculum produced by the degradation bacterium, pertaining to the high biotechnology field, wherein, the applied strains are Gram-stain negative bacteria Ld1 which are identified as Pseudomonas sp.; the degradation bacterium is mainly and biologically characterized in that: the main biological characteristic is G<->; thallus takes the shape of short rods, and the size of the thallus is about 0.5 Mum to 1.0 Mum multiplied by 1.5 Mum to 5.0 Mum; oxidase, catalase, nitrate reduction and citrate utilization reactions are positive; lipase reactions, V.P. reactions, MR reactions, indophenol reactions are negative; and starch can not be hydrolyzed. The microbial inoculum is obtained after the completion of the fermentation of the Ld1 strains. The application of the degradation bacterium products can reduce the residual quantity of butyl 2, 4-dichlorophenoxy acetate pesticides by more than 70 percent and can solve the problem that butyl 2, 4-dichlorophenoxy acetate pesticide residue in the agricultural production exceeds the standard.

Description

1. Technical field

[0001] The invention provides a 2,4-dichlorophenoxy butyl ester pesticide residue degrading bacteria and the bacterial agent produced therefrom, belonging to the field of biological high technology, which uses microorganisms to degrade chemical pesticide residues, and is suitable for green production in modern agricultural production. Production of pollution-free agricultural products. 2. Background technology

[0002] The use of chemical pesticides has greatly promoted the development of modern agriculture, restored a large number of losses caused by plant diseases, insects, and weed damage for agricultural production, and greatly improved the labor efficiency and mechanization degree of agricultural production. played an invaluable role in the modernization process. But at the same time, pesticides are some toxic and harmful chemical substances. After they enter the ecological environment, they not only kill the organisms that are harmful to humans, but...

Examples

Embodiment Construction

[0013] The invention provides a degrading bacterial agent for eliminating 2,4-dichlorophenoxybutyl acetate pesticide residues. The bacterial strain used is Gram-negative bacteria Ld1, which is isolated from 2,4-dichlorobenzene, which has been used for a long time in Tai'an City, Shandong Province. Farmland soils with butyl oxyacetate pesticide.

[0014] On July 23, 2008, it was preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, the strain preservation number is: CGMCC NO.2601, and it was identified as Pseudomonas sp. The main biological characteristic is G - , the bacteria are short rod-shaped, the size is 0.5-1.0×1.5-5.0μm, oxidase, catalase, nitrate reduction, citric acid utilization reactions are positive; lipase reaction, V.P. reaction, M.R reaction, indole reaction are Negative; unable to hydrolyze starch.

1. Technical field

[0001] The invention provides a 2,4-dichlorophenoxy butyl ester pesticide residue degrading bacteria and the bacterial agent produced therefrom, belonging to the field of biological high technology, which uses microorganisms to degrade chemical pesticide residues, and is suitable for green production in modern agricultural production. Production of pollution-free agricultural products. 2. Background technology

[0002] The use of chemical pesticides has greatly promoted the development of modern agriculture, restored a large number of losses caused by plant diseases, insects, and weed damage for agricultural production, and greatly improved the labor efficiency and mechanization degree of agricultural production. played an invaluable role in the modernization process. But at the same time, pesticides are some toxic and harmful chemical substances. After they enter the ecological environment, they not only kill the organisms that are harmful to humans, but...

Examples

Embodiment Construction

[0013] The invention provides a degrading bacterial agent for eliminating 2,4-dichlorophenoxybutyl acetate pesticide residues. The bacterial strain used is Gram-negative bacteria Ld1, which is isolated from 2,4-dichlorobenzene, which has been used for a long time in Tai'an City, Shandong Province. Farmland soils with butyl oxyacetate pesticide.

[0014] On July 23, 2008, it was preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, the strain preservation number is: CGMCC NO.2601, and it was identified as Pseudomonas sp. The main biological characteristic is G - , the bacteria are short rod-shaped, the size is 0.5-1.0×1.5-5.0μm, oxidase, catalase, nitrate reduction, citric acid utilization reactions are positive; lipase reaction, V.P. reaction, M.R reaction, indole reaction are Negative; unable to hydrolyze starch.