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218 results about "Nitrate reducing" patented technology

Nitrate reduction test is a test that determines the production of an enzyme called nitrate reductase, which results in the reduction of nitrate (NO3). Bacterial species may be differentiated on the basis of their ability to reduce nitrate to nitrite or nitrogenous gases. To determine the ability of an organism to reduce nitrate to nitrite.

Method for strengthening indigenous microbes and improving oil recovery by improving microbial florae in oil deposit

The invention provides a method for strengthening indigenous microbes and improving oil recovery by improving microbial florae in an oil deposit. The method comprises the following steps of: (1) analyzing composition of microbial florae in the oil deposit; and (2) introducing exogenous microbes into the oil deposit according to an analysis result, so that the oil deposit with the introduced exogenous microbes contains the following microbes: hydrocarbon-oxiding bacteria, fermentative bacteria, heterotrophic bacteria, methanogenic bacteria, sulphate-reducing bacteria, and nitrate-reducing bacteria. Due to the adoption of the method provided by the invention, by adding the exogenous microbes into the oil deposit, the structure of the microbial florae in the oil deposit is improved, the function of the microbial florae in the oil deposit is strengthened, as a result, the oil displacement effect is improved, and the application range of microbial oil recovery technology is enlarged.
Owner:INST OF MICROBIOLOGY - CHINESE ACAD OF SCI

Method for enhanced nitrogen removal of tidal flow-horizontal subsurface flow hybrid constructed wetland and system thereof

The invention belongs to the technical field of sewage treatment and specifically relates to a method for enhanced nitrogen removal of a tidal flow-horizontal subsurface flow hybrid constructed wetland and a system thereof. The system orderly comprises a non-powered automatic water intake device (8), a tidal-flow constructed wetland bed, a non-powered automatic water drainage device (2) and a horizontal subsurface-flow constructed wetland bed. The tidal-flow constructed wetland which is high in ammonia nitrogen oxidation effect and the horizontal subsurface-flow constructed wetland which is excellent in nitrate reduction capability are rationally combined into one system by thoroughly utilizing the characteristics of the aerobic environment of the former and the anaerobic environment of the latter; therefore, conditions are created for thorough nitration / denitrification; and simultaneously, a horizontal subsurface-flow wetland slow-release carbon source enhanced region (15) and a backflow well (19) are arranged in the horizontal subsurface-flow wetland bed, so that enhanced removal of total nitrogen in the sewage can be realized and secondary pollution on the carbon source is avoided at the same time.
Owner:CHINA AGRI UNIV

Method for treating high-concentration nitrate waste water and municipal sewage through denitrification anaerobic ammonium oxidation SBR

The invention provides a method for treating high-concentration nitrate waste water and municipal sewage through denitrification anaerobic ammonium oxidation SBR, belonging to the field of sewage biotreatment. The method comprises the following steps: firstly, culturing and acclimating denitrifying bacteria population and anaerobic ammonium oxidation bacteria population which have inadequate denitrification characteristic, then inoculating anaerobic ammonium oxidation sludge and denitrifying sludge with high nitrite accumulation rate in the same reactor, leading nitrate waste water and municipal sewage to flow in denitrification anaerobic ammonium oxidation SBR reactor at a certain ratio, performing anaerobic ammonium oxidation to nitrite accumulated during reduction of nitrite and ammonium nitrogen in the municipal sewage to generate nitrogen gas and synchronously removing. According to the method, nitrate generated during anaerobic ammonium oxidation can be reduced through denitrifying bacteria without the condition of adding a carbon source, the nitrogen removal efficiency of a system can be improved, and the effective route for realizing sewage nitrogen removal with high efficiency and low energy consumption can be provided.
Owner:BEIJING UNIV OF TECH

Laterosporo short bacillus, and its fermentating process and use

The invention provides a kind of Brevibacillus laterosporus (STP-B.latCGMCCNo.1119), the character follows this. G+, haulm shape, the shape is (0.4-0.5)*(2-2.5)mu. The spore adnation is from neutrality to sub-neutrality. The spore bursa will enlarge. Most of the spore is vertebra aitch, the remainder is disciform. VP-, ph value of culture fluid is 7.2, amylolysis-, gelatin dispergation-, decomposition casein+, katalase+, oxidase-, cirate -, dextrose aerogenesis-, dextrose acidgenesis+, L-arabinose acidgenesis-, xylose acidgenesis-, mannit acidgenesis+, azotate deocidisation+, methyl red+, pH5.7 growth+.
Owner:SHENGTAIPING BIOLOGICAL ENG TECH BEIJING

Device and method for treating low-carbon-nitrogen-ratio urban sewage by virtue of half nitrification/partial denitrification/anaerobic ammonium oxidation

The invention discloses a device and method for treating low-carbon-nitrogen-ratio urban sewage by virtue of half nitrification / partial denitrification / anaerobic ammonium oxidation, belonging to the field of sewage treatment. The device comprises an urban domestic sewage tank, a half nitrification reactor, a first intermediate water tank, a partial denitrification reactor, an external carbon source storage tank, a partial denitrification online monitoring and control system, a second intermediate water tank and an anaerobic ammonium oxidation reactor. The method comprises the following steps: firstly introducing domestic sewage into the half nitrification reactor, oxidizing part of ammonia nitrogen in the domestic sewage into nitrate nitrogen by virtue of aeration, introducing effluent into the partial denitrification reactor, reducing nitrate into nitrite by using a small amount of an external carbon source, and introducing the effluent into the anaerobic ammonium oxidation reactor for denitrification. By adopting the device and the method disclosed by the invention, stable and efficient denitrification of the low-carbon-nitrogen-ratio urban sewage can be achieved, and the problems that the existing short-cut nitrification nitrite accumulation of the urban domestic sewage is unstable and an existing anaerobic ammonium oxidation process is low in denitrification efficiency can be solved.
Owner:BEIJING UNIV OF TECH

Achromobacter capable of degrading aniline and application thereof

The invention discloses an achromobacter capable of degrading aniline, wherein the 16S rDNA of the achromobacter has a sequence of SEQ ID No.1. Colonies are yellow, round, smooth and humid with neat edges and convex bulges. Through electron microscope observation, the achromobacter does not have flagellum and spore bacteria; and under the observation of the electron microscope, the form of the achromobacter is a thicker bacillus which is gram staining negative and oxidase and catalase negative, and is reduced by nitrate to generate gas. The achromobacter can achieve the efficient degradation of aniline pollutants under an aerobic condition, the maximum tolerance concentration to the aniline is up to 4,500 milligrams per liter, the aniline of which the concentration is less than or equal to 4,000 milligrams per liter can be completely removed, thus the achromobacter plays an important role in the controlling practice of aniline industrial wastewater.
Owner:ZHEJIANG UNIV OF TECH

Method of using native function microflora to in-situ purify ground water polluted by uranium

The invention relates to a method of using native function microflora to in-situ purify ground water polluted by uranium. The method translates transferable hexavalent uranium into nontransferable uranous uranium in the ground water through nitrate reducing bacteria flora, ferric iron reducing bacteria flora and sulfate reducing bacteria flora which are formed under the anaerobic condition by microorganism, and therefore the purpose of purifying the ground water polluted by the uranium is achieved. The specific implementation method comprises: (1) adjusting a potential of hydrogen (Ph) value of the ground water polluted by the uranium; (2) activating the microorganism in sediments; (3) regulating and controlling variation of the microflora, accelerating evolution of the nitrate reducing bacteria flora, the ferric iron reducing bacteria flora and the sulfate reducing bacteria flora by means of adding a carbon source, regulating oxidation reduction potential of the water polluted by the uranium and the like; (4) continuously regulating and controlling the microbial flora until uranium concentration in the ground water polluted by the uranium is reduced below the national emission standard and the purpose of purifying the groundwater polluted by the uranium is achieved. The method of using the native function microflora to in-situ purify the ground water polluted by the uranium solves in-situ purifying problem of the ground water polluted by the uranium, and has the advantages of being high in purifying efficient, short in period, simple in equipment, short in process procedure, low in purifying cost, strong in operability, free of secondary pollution, good in purifying effect and the like.
Owner:NANHUA UNIV

Streptococcus thermophilus separated from tibetan kefirs as well as separation method and applications thereof

The invention discloses streptococcus thermophilus separated from tibetan kefirs as well as a separation method and applications thereof, belonging to the field of microbial technology. The separation method is characterized in that an operation of streaking separation is performed on a M17 culture medium plate, an operation of culturing is performed 48 hours at a temperature of 42 DEG C, a bacterial colony is circular and raised, neat in edge and milk white in color, bacterial colonies which are non-transparent, contain spherical bacteria, can be subjected to wiredrawing, provided with wet and smooth surface, and mostly arranged in chains with different lengths are selected, and no spore is produced; the streptococcus thermophilus is negative in a catalase test, positive in gram stain, negative in a nitrate reduction test, and has no motility and aerobic and facultative anaerobic growth, gelatin is not liquefied, hydrogen sulfide is not produced, and the streptococcus thermophilus is negative in an indole test; the streptococcus thermophilus does not grow at a temperature of 15 DEG C; the growth temperature is 37-45 DEG C; the highest and lowest initial growth pH values are respectively 9.0 and 4.0, and the growth initial pH value is 6.5-7.0. The streptococcus thermophilus aas well as separation method and applications thereof disclosed by the invention have the beneficial effects that the maximum quantity of exopolysaccharides produced when the streptococcus thermophilus is fermented 30 hours by using a M17 culture solution under the condition of 42 DEG C is 111 mg/L; and when a fermented yoghurt is prepared by using strains provided by the invention, the amounts of a thickener and a stabilizer can be reduced.
Owner:JILIN ACAD OF AGRI SCI
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