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Laterosporo short bacillus, and its fermentating process and use

A technology of Brevibacillus brevis and lateral spores, which is applied to newly isolated microorganisms and their application fields, can solve problems such as hindering the popularization, use and development of biological fertilizers, poor salt resistance, and incompatibility.

Inactive Publication Date: 2005-02-16
SHENGTAIPING BIOLOGICAL ENG TECH BEIJING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A small number of whole spores also seriously hinder the promotion, use and development of biological fertilizers due to the incompatibility of inorganic fertilizers and poor salt resistance.

Method used

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  • Laterosporo short bacillus, and its fermentating process and use
  • Laterosporo short bacillus, and its fermentating process and use
  • Laterosporo short bacillus, and its fermentating process and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Isolation and screening of Brevibacillus laterosporus (Brevibacillus laterosporus) STP-B.lat

[0048] Isolation and Screening of Brevibacillus Laterosporus STP-B.lat:

[0049] 1) 1) Medium used for the isolation and purification of Brevibacillus lateralsporosa STP-B.lat:

[0050] The isolation and purification of Brevibacillus lateralsporogen STP-B.lat is mainly based on the improved Dǒbereiner medium, supplemented by mannitol medium and improved Ashby medium, and the formulations of each medium are as follows:

[0051] Modified Dǒbereiner medium:

[0052] Raw material amount Raw material amount

[0053] Sucrose 10.0g Apple juice 5.0g

[0054] K 2 HPO 4 ·H 2 O 0.1g K 2 HPO 4 ·H 2 O 0.4g

[0055] MgSO 4 ·7H 2 O 0.2g NaCl 0.1g

[0056] CaCl 2H 2 O 0.02g FeCl 3 0.01g

[0057] Na 2 MoO 4 ·H 2 O 0.002g H 2 O 1000ml

[0058] Adjust the pH to 7.2 with NaOH.

[0059]NN:...

Embodiment 2

[0084] The growth-promoting nitrogen-fixing function of embodiment 2 Brevibacillus laterosporus (Brevibacillus laterosporus) STP-B.lat bacterial strain:

[0085] Acetylene reduction method:

[0086] (1) Determination method: Add 4.5ml of modified Dǒ's low-nitrogen solid medium into a 15×150mm test tube, autoclave at 121°C for 15 minutes, and make a slope. After inoculation, culture at 30°C for 18-24hrs, replace the cotton plug with a rubber stopper, extract 1.8ml of air, and inject 1.6ml of C 2 h 2 After continuing to cultivate for 24-36hrs, take 100 gas samples and measure the ethylene peak value on the SQ-204 gas chromatograph, standard gas C 2 h 2 The concentration is 138mg·ml -1 . The working conditions of the gas chromatograph are set as follows: the temperature of the detection chamber is 100°C, the temperature of the column is 60°C, the temperature of the injection port is 60°C, the length of the column is 1m, and the pressure of nitrogen at the head of the meter...

Embodiment 3

[0134] Determination of Phosphorus, Potassium, Nitrogen Fixation Effectiveness and Other Functions of Example 3 Brevibacillus laterosporus (Brevibacillus laterosporus) STP-B.lat

[0135] Determination of Phosphorus Solubilizing Potency

[0136] raw material

quantity

raw material

quantity

raw material

quantity

glucose

10g

(NH 4 ) 2 SO 4

0.5g

NaCl

0.3g

KCl

0.3g

MgSO 4

0.3g

FeSO 4

0.03g

MnSO 4

0.03g

yeast

0.5g

CaCO 3

0.1g

Lecithin

0.2g

distilled water

1000ml

Phosphate rock powder

0.1％

[0137] Note: adjust pH=7.0~7.5

[0138] Divide them into Erlenmeyer flasks for sterilization, inoculate the bacteria for determination, culture with shaking at 30°C for 4 days, filter, and measure available phosphorus by plasma radiation spectrometry. Phosphorus solubilization was 176.58μg / ml, which was 17.1 times higher ...

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PUM

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Abstract

The invention provides a kind of Brevibacillus laterosporus (STP-B.latCGMCCNo.1119), the character follows this. G+, haulm shape, the shape is (0.4-0.5)*(2-2.5)mu. The spore adnation is from neutrality to sub-neutrality. The spore bursa will enlarge. Most of the spore is vertebra aitch, the remainder is disciform. VP-, ph value of culture fluid is 7.2, amylolysis-, gelatin dispergation-, decomposition casein+, katalase+, oxidase-, cirate -, dextrose aerogenesis-, dextrose acidgenesis+, L-arabinose acidgenesis-, xylose acidgenesis-, mannit acidgenesis+, azotate deocidisation+, methyl red+, pH5.7 growth+.

Description

technical field [0001] The present invention relates to a newly isolated microorganism and its application, more specifically, relates to a Brevibacillus laterosporus (Brevibacillus laterosporus) STP-B.lat and its application. Background technique [0002] The use of chemical fertilizers has brought agricultural production to a great level, but with the increasing use of fertilizers, it has brought about negative effects such as soil compaction, environmental pollution, and decline in crop quality. Therefore, biological fertilizers, especially inorganic, organic and biological fertilizers have become a promising new generation of ecological fertilizers. However, most of the bio-fertilizers currently on the market are not fully spores due to the poor functionality of the effective bacteria used. The cultivation and production process is rough and simple, the survival period of biological bacteria is short, it is difficult to store, the quality and efficiency of the fertilize...

Claims

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Application Information

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IPC IPC(8): C05F11/08C12N1/20
Inventor 曾飞蔡妙英
Owner SHENGTAIPING BIOLOGICAL ENG TECH BEIJING
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