High-bile salt resistance strain and bile salt hydrolase genes

A technology of bile salt hydrolysis enzyme and bile salt resistance, applied in the field of related genes

Active Publication Date: 2014-02-12
哈尔滨美华生物技术股份有限公司
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  • Abstract
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Problems solved by technology

However, according to the experimental results, it is speculated that the mechanism of lactic aci

Method used

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  • High-bile salt resistance strain and bile salt hydrolase genes
  • High-bile salt resistance strain and bile salt hydrolase genes
  • High-bile salt resistance strain and bile salt hydrolase genes

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Embodiment Construction

[0026] Below in conjunction with the examples, the present invention is further described, the following examples are illustrative, not limiting, and the protection scope of the present invention cannot be limited by the following examples.

[0027] The following examples illustrate the technical scheme of the present invention from the three aspects of the acquisition of bacterial strain, the isolation of the bacterial strain bile salt hydrolase gene and the expression of bile salt hydrolase, the specific contents are as follows:

[0028] 1. Isolation and identification of L.plantarumCGMCCNo.8198

[0029] The following experiments were performed under sterile conditions.

[0030] 1. Enrichment of strains

[0031] Weigh 5g of silage, add 20mL of sterile saline to homogenize, take 100μL of the obtained homogenate after appropriate dilution, spread on the MRS plate containing 0.3% pig bile salt, and incubate in an anaerobic incubator at 37°C for 36-72h , to observe the growth ...

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Abstract

The invention discloses a high-bile salt resistance strain and bile salt hydrolase genes. The preservation number of the strain is CGMCCNo.8198. The strain has high acid resistance, high bile salt resistance, high nitrate reducing capability and the like. Three types of bile salt hydrolase genes of the strain are amplified, and are cloned, sequenced and heterogeneously expressed, so that generated bile salt hydrolases have high activity. The strain and the bile salt hydrolases can be used in the fields of food, medicine, agriculture and the like as food and feed leavening agents, microecological preparations, medicament expression carriers, feed additives and the like; the bile salt hydrolases can be developed into recombinant protein products, and can also be used for the molecular modification of lactic acid bacteria strains as selection markers of lactic acid bacteria gene engineering expression systems.

Description

technical field [0001] The invention belongs to the field of bioengineering, and relates to a new bacterial strain of the genus Lactobacillus producing bile salt hydrolyzing enzyme and related genes isolated from the bacterial strain. Background technique [0002] Probiotics refer to a single or specific mixture of microorganisms that can reach the gastrointestinal tract in the state of live bacteria when ingested in a certain amount, and play a role in promoting human or animal health by regulating the composition of intestinal flora. As important physiological beneficial bacteria in the gastrointestinal tract of humans and animals, probiotics have the functions of maintaining the normal micro-ecological balance of the gastrointestinal tract, inhibiting the invasion and infection of pathogenic bacteria, enhancing the body's immunity, preventing and inhibiting tumorigenesis, lowering cholesterol and anti-oxidation. And so on. Probiotics are still dominated by lactic acid ba...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N15/55C12N9/14C12R1/25
Inventor 张同存罗学刚古向超张瑶王重喜
Owner 哈尔滨美华生物技术股份有限公司
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