9 results about "Nitrate reductase" patented technology

The invention discloses a molybdenum oxide nanorod material, a preparation method of the molybdenum oxide nanorod material and application of the molybdenum oxide nanorod material to improvement of corn nutrient utilization, growth promotion and lodging resistance, and belongs to the technical field of corn nutrient utilization and agricultural nano material application. The morphology and dispersity of the nanorod are optimized by combining molecular composition characteristics of key enzymes (such as nitrate reductase and glutamine synthetase) in a corn nutrient utilization process, and finally the functional nanorod with targeting property and biocompatibility is obtained and can be widely applied to nutrient regulation and control scenes of corn water culture and field planting. After the material is applied, the activity of nitrate reductase and glutamine synthetase of a corn root system can be activated in a targeted manner, the nutrient utilization efficiency is accurately improved, and sufficient nutrition support is provided for plant growth. After the fertilizer is applied to plants, the root system is promoted to be developed, the stalks are thick and strong, the lodging resistance is remarkably enhanced, the protein content of the plants and grains can be increased, and the synergistic effects of efficient nutrient utilization, robust plant growth, lodging resistance and yield increase are achieved.

The invention relates to the technical field of agricultural resources and environment, in particular to a fertilization method for reducing nitrogen loss of an apple orchard. The apple branch biochar is prepared from the apple orchard pruning waste, and resource utilization of the waste is achieved. Through the synergistic effect of the apple branch charcoal, dicyandiamide and the reduced nitrogen fertilizer, the loss of nitrate nitrogen in soil is obviously slowed down, the conversion of ammonium nitrogen is inhibited, and a nitrogen cycle functional microflora is regulated, so that the application amount of the nitrogen fertilizer is reduced, the utilization rate of the nitrogen fertilizer is improved, and the environmental pollution is reduced; the growth of apple plants can be obviously promoted, the root configuration is improved, and the root activity and the nitrate reductase activity are improved.

The invention belongs to the technical field of microorganisms, and particularly relates to bifidobacterium longum subsp. Infantis, a probiotic preparation and application of the probiotic preparation in products for reducing blood sugar and blood lipid. The bifidobacterium longum subsp. Infantis provided by the invention is bifidobacterium longum subsp. Infantis A32-03, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.32811. The strain does not generate hemolysin, does not have nitrate reductase activity, does not generate any harmful metabolites such as biogenic amines and indoles, is sensitive to various antibiotics, and is good in biological safety. Particularly, the probiotic preparation obtained by fermenting the bifidobacterium longum subsp. Infantis A32-03 in a culture medium containing the auricularia auricula polysaccharide has a prominent inhibition effect on alpha-amylase, alpha-glucosidase and lipase, has an excellent degradation capability on cholesterol, has a good blood sugar and blood fat regulation effect, and can be used for preparing the probiotic preparation. The method has good popularization value in oral administration of medicines for reducing blood sugar and blood fat or health-care food for regulating blood sugar and blood fat.

This invention belongs to the field of crop breeding, specifically relating to an NtLHT1 gene and its application in tobacco's resistance to low nitrogen stress. It involves artificially intervening in the mutation of specific nucleotide sequence fragments in the NtLHT1 gene to obtain two mutated nucleotide sequence fragments, resulting in a mutant NtLHT1 gene, and the application of the mutant NtLHT1 gene in improving tobacco's resistance to low nitrogen stress. The NtLHT1-edited tobacco plants of this invention exhibit enhanced absorption of nitrate and ammonium nitrogen during their vigorous growth period, increased activity of nitrate reductase and glutamine synthase, increased expression of nitrogen transporter genes, and improved nitrogen utilization efficiency. This invention increases the activity of nitrate reductase and glutamine synthase, thereby improving tobacco's resistance to low nitrogen stress and providing a genetic resource and material basis for the development of nitrogen utilization-related genes and strains in tobacco.

The present application relates to the technical field of microbial food fermentation, and specifically discloses a Staphylococcus simulans HZ01 inoculant, preparation of the Staphylococcus simulans HZ01 inoculant comprising the following steps: (A) inoculating Staphylococcus simulans HZ01 into NB culture medium for culture to obtain a bacterial solution; (B) when the concentration of Staphylococcus simulans HZ01 in the bacterial solution obtained in step (A) is 1.0×10 8 log 10 CFU / mL to 9.9×10 9 log 10 CFU / mL, centrifugation is performed; (C) after completion of the centrifugation, sterilized skimmed milk is added for resuspension; (D) after completion of the resuspension, freeze-drying is performed to obtain a powdered Staphylococcus simulans HZ01 inoculant with a concentration of 1.0×10 10 log 10 CFU / g to 9.9×10 11 log 10 CFU / g. The present application has the characteristics of good processing adaptability, high protease and lipase activity, good nitrate reductase activity, and good flavor of fermented meat products.

The application discloses a rice OsEIL1 gene RNAi expression vector and application thereof. The RNAi expression vector of the rice OsEIL1 gene is expressed in rice, and the RNAi expression vector can inhibit the expression amount of the OsEIL1 gene in the rice root, that is, specifically inhibit the expression of the OsEIL1 gene in the rice root, thereby improving the nitrogen fertilizer utilization efficiency and yield of the rice. The RNAi expression vector of the rice OsEIL1 gene is expressed in rice, so that the expression amount of the OsEIL1 gene in the rice root is reduced, thereby improving the nitrogen fertilizer utilization efficiency and yield of the rice. Test results prove that, compared with wild-type rice with normal expression of the OsEIL1 gene, transgenic rice containing the RNAi expression vector has significantly increased aboveground biomass, grain length, 1000-grain weight and nitrate reductase activity under low-nitrogen and high-nitrogen conditions. Meanwhile, compared with wild-type rice with normal expression of the OsEIL1 gene, the transgenic rice containing the RNAi expression vector has more increased yield per plant under low-nitrogen conditions.

The invention discloses a micro-ecological fermented feed suitable for piglets and a preparation method of the micro-ecological fermented feed, and belongs to the technical field of functional feeds. The preparation method comprises the following steps: 1) preparing functional hydrolysate rich in arginine and branched chain amino acid from plant raw materials through composite enzymolysis; (2) inoculating a functional bacterial agent consisting of lactobacillus plantarum with high arginase activity, nitrate reductase positive bacillus subtilis and clostridium butyricum; (3) accurately controlling the fermentation process by taking the molar ratio of arginine to citrulline being 0.8-1.5 as a fermentation end point, directionally enriching nitric oxide precursors such as citrulline in the feed, and constructing an endogenous NO slow-release system; the product can safely and efficiently activate NO metabolism of intestinal tracts of piglets, synergistically improve intestinal barrier, flora balance, digestive function and whole body immunity, significantly increase daily gain, reduce diarrhea rate, replace zinc oxide and antibiotics, and realize green and healthy breeding.

The invention relates to an industrialized production method of green and fresh-keeping instant vegetables, which comprises the following steps: washing pollution-free green hydroponic green vegetables newly harvested by Shandong Weifang Vegetable Primary Company, carrying out high-quality vacuum packaging by using a high-temperature cooking vacuum packaging food bag, putting into a division mesh frame, and carrying out vacuum packaging by using a high-temperature cooking vacuum packaging food bag, so as to obtain the green and fresh-keeping instant vegetables. The green vegetables are hung into a special cooking pot to be tightly covered and sealed, the green vegetables are immediately and rapidly heated to be cooked until the green vegetables are completely cooked or secondarily cooked, then the green vegetables are depressurized and uncovered, the green vegetables are hung into a rapid cooling barrel to be rapidly cooled, and then the green vegetables are hung out to obtain finished products, namely the instant green vegetables or the instant cooked green vegetables. The green vegetables prepared by the technology disclosed by the invention are steamed at high temperature after being packaged in vacuum, and various bacteria and nitrate reductase are killed, so that the green vegetables do not deteriorate after being refrigerated for 6 months at normal temperature and 12 months. As no additive or any side effect is contained, the food is healthy, the preparation method is simple, and factory-like batch production can be realized. Wherein the fully-cooked green vegetables are mixed with trace elements and seasoning bags and are ready to eat, so that the vegetable sauce is rich in nutrition, very convenient and particularly suitable for office workers and groups in high-intensity living environments such as high-cold frontier defense, island naval vessels and the like; wherein the second-mature instant boiled green vegetables are especially suitable for anti-season storage, long-distance transportation and sales, foreign trade transactions and the like due to long shelf life, so that the requirements are very wide.

The application discloses a kind of cucurbitaceae sphingomonas SPZ1 and its complex microbial inoculant in application of promoting peanut plant photosynthetic physiology and nitrogen metabolism, belong to the field of microbial technology.The active bacteria of the present application is first composed of plant Arthrobacter ARZ1 (Arthrobacter oryzae) and cucurbitaceae sphingomonas SPZ1 (Sphingomonas melonis) complex microbial inoculant is applied to peanut planting field, the two bacteria in the complex microbial inoculant have obvious improvement on the maximum photochemical efficiency of peanut plant and leaf chlorophyll SPAD value through synergistic effect, especially can improve the activity of whole peanut leaf nitrogen metabolism enzyme (nitrate reductase and glutamine synthetase), so as to be applied to promote peanut plant photosynthetic physiology and nitrogen metabolism.