89 results about "Nitrate reductase" patented technology

A material includes a surface and a reactive agent that is located at the surface of the material, covalently attached to a backbone of the material, and/or located within the material. The reactive agent has nitrite reductase activity, nitrate reductase activity, and/or nitrosothiol reductase activity. The reactive agent also converts at least one of nitrites, nitrates and nitrosothiols to nitric oxide when in contact with blood. A reproducible nitrosothiol sensor is also disclosed.

The invention relates to a nitrogen-fixing bacillus megaterium strain DL7 and application thereof. The strain is preserved in the General Microbiological Center of the China Committee for Culture Collection of Microorganisms in June 7, 2010 and has a preserving number as CGMCC NO.3914. The strain is a new nitrogen-fixing strain obtained by using the conventional microorganism separation and purification method for Chinese podocarpus root nodules. In the strain, the nitrogenase activity is 1.5632nmol.g<-1>.h<-1>; after an eucalyptus seedling is planted, the seedling height increases 40.78-56.22 percent, the ground diameter increases 39.41-46.43 percent, the average biomass increases 72.11-158.76 percent, the chlorophyll content increases 12.97-20.98 percent, and the nitrate reductase activity increases 33.82-35.22 percent. The strain can be applied to a biological bacterial fertilizer for promoting eucalyptus seedling growth.

Probiotic compositions, particularly oral compositions, comprising one or more bacteria that are capable of producing nitrite and/or nitric oxide in a subject are provided. Some compositions further comprise nitrate/nitrate, such as a botanical source of nitrate. Some compositions further comprise botanical sources of nitrate reductase. Also, provided are methods of improving the oral and/or vascular health of a subject by orally administering a composition comprising one or more bacteria capable of producing nitric oxide. Methods of repopulating the nitric oxide producing microflora and bacterial environment in the oral cavity of a subject are also provided.

The invention belongs to the field of microorganisms, and discloses a strain of pseudomonas stutzeri ZH-14 with an aerobic denitrification function. The preservation number of the pseudomonas stutzeriZH-14 is CCTCC NO:M2018730. The pseudomonas stutzeri can be applied to degrading ammonia nitrogen or nitrate nitrogen or nitrite nitrogen or COD in nitrogen-containing sewage. The pseudomonas stutzeri ZH-14 has the advantages that the pseudomonas stutzeri ZH-14 has the aerobic denitrification function and a heterotrophic nitrification function, and the accumulation of the nitrite nitrogen with the extremely low content is involved in the degradation process; nitrite can be directly utilized for growth metabolism, the activity of nitrate reductase of the pseudomonas stutzeri ZH-14 is still high in an environment with a low carbon-nitrogen ratio, the aerobic expression is less influenced by the types of adopted carbon sources, and the carbon source problem in the process of utilizing aerobic denitrification bacteria for treating nitrogen-containing wastewater is solved.

The invention relates to a nitrogen fixation stenotrophomonas maltophilia C4Y41 strain and an application thereof. The strain is stored in the common microorganism center of China microorganism strain management committee and has a storage number of CGMCC No.3349. The strain is a new nitrogen fixation strain obtained by applying a general microorganism-separating and purifying method on podocarpus root nodules, has the nitrogen fixation enzyme activity of 0.019-0.030nmol*g-1*h-1 and enables the height of inoculated eucalyptus seedlings to increase by 2.20-22.12 percent, the ground diameter to increase by 22.15-36.84 percent, the average biomass to increase by 40.47-60.81 percent, the chlorophyll content to increase by 12.68-34.07 percent and the nitrate reductase activity to increase by 21.82-36.69 percent. The strain can be applied as a biological fertilizer for stimulating the growth of the eucalyptus seedlings.

The invention constructs an expression vector suitable for expressing an exogenous gene in a chlorella nitrate reductase deletion mutant by using a Ubiquitin promoter and an omega enhancer as starting elements and the nitrate reductase gene NR. An objective gene is converted to the mutant by using an economic and simple chlorella PEG conversion method and employing the Chlorella ellipsoidea nitrate reductase deletion mutant as a receptor. The transgene chlorella wall breaking technology is optimized, the expressed exogenous gene has normal biological activity and is stably inherited in a culture medium without antibiotics. The results of the invention can high efficiently and safely be used to express the exogenous gene which does not suit to be expressed by the normal genetic engineering expression system (such as the escherichia coli and yeast) by using the genetic engineering technology and the Chlorella ellipsoidea nitrate reductase deletion mutant as a vector.

The invention provides a fertilizer containing carbon, in particular ammonium bicarbonate, which is added with 0.1 to 1 weight percent of 5-to-200-nanometer carbon nano material, preferably 0.1 to 0.5 weight percent of 5-to-100-nanometer carbon nano material, and 0.2 to 0.7 percent of ammonia stabilizer or nitrate reductase inhibitor. The compound fertilizer has obvious emission-reducing and synergetic effects.

The invention adds anion additives capable of releasing anions and emitting far infrared rays into fertilizer cladding or fertilizer; natural mineral nanometer materials and the natural mineral nanometer technology are applied and organically combined with the slow release fertilizer technology; the nanometer technology and chemical and physical molecule design principles are adopted; and the metabolism of plants is promoted by increasing the energy and the activity of water and fertilizer. Moreover, the invention has the functions of promoting the vitality of crop roots and the activity of nitrate reductase and the like, and increases the abilities of the plants for absorbing liquid fertilizer and utilizing nitrogen fertilizer, thereby saving the consumption of the fertilizer.

The invention relates to a tobacco floating growing substrate and a preparation method thereof. The tobacco floating growing substrate comprises 65-80 percent of rotten peanut chaff, 10-20 percent of expanded perlite and 10-20 percent of vermiculite. The preparation method of the tobacco floating growing substrate comprises the following steps of: removing impurities from peanut shells and crushing the peanut shells to obtain peanut chaff of 3-6mm; adjusting the obtained peanut chaff until the water content is 60-70 percent; adding an HM (high methoxyl pectin) decomposition agent and uniformly mixing the HM decomposition agent with urea for stack retting; maintaining the temperature in the decomposition high-temperature stage to be 55-65DEG C; when the temperature in the stack is risen to over 65DEG C, turning stacks and reducing the temperature in time; when the temperature in the stack is recovered to normal temperature without raising, obtaining the rotten peanut chaff, sterilizing the rotten peanut chaff and uniformly mixing the rotten peanut chaff with the expanded perlite and the vermiculite according to the mixture ratio to form the tobacco floating growing substrate. The tobacco floating growing substrate has the advantages of moderate granularity, looseness and permeability, formed aggregated structure, favorable permeability and water retention property and stable physical and chemical properties; and the activities of tobacco nitrate reductase and saccharase can be improved and the development of a tobacco root system is promoted.

The invention is an double-starter DNA vaccine expression carrier pCMVnir and its preparing method. The expression carrier pCMVnir is double stranded closed-loop molecule, a gene cloning carrier, and the length is 5.1Kb, used to express to prepare genetic engineering product and produce DNA vaccine, containing two starters, one is an eukaryoti cell starter (HCMV IE), and the other is an escherichia coli nitratase gene starter nirB, the two starters are activated in cell and prokaryotic cell to express the corresponding proteins, respectively, it contains polyclonal restriction site where the external source is inserted, and the carrier has the sequence shown in SEQ ID NO 1.It can be widely used to high-efficacy expression of external source gene and need not add inducer to the culturing system.

Methods for increasing the levels of lipids in oleaginous algae are described. Lipid levels in algae can be increased by stress, such as nutrient stress, after which the lipid can be harvested from the algae using a non-destructive extraction process. The stress may be provided in a periodic or “pulsed” fashion. Lipid levels in oleaginous algae can also be increased using simulated stress by treating the algae with a chemical inhibitor or by using recombinant technology to insert a sequence expressing a protein such as a nitrate reductase inhibitor that is expressed when a stressed state is desired. A method for maintaining the temperature and water levels of algae ponds using buoyant spheres is also described.

The invention relates to an analysis method of testing the nitrate reductase activity in the soil, which includes the following steps: 1) n dried and screened soil samples are weighed, adopted and put into test tubes; 2, 4-dinitrophenol solution is added into the test tubes and mixed to be even; and then substrate potassium nitrate solution is added into n-1 test tubes and the same amount of distilled water is added into the other one test tube for sample comparison; glucose solution is respectively added into the test tubes, which is then supplemented by distilled water to be 5 to 10ml and cultivated under constant temperature; 2) the distilled water is added to supplement to 50 to 100ml; 3) potash alum saturated solution is added in to be oscillated and filtered; 4)the distilled water and a chromogenic reagent are added into the filtrate, and a light absorption value A is determined under 520nm; 5) the content of nitrite in the samples is calculated. The analysis method has the advantages of: 1) simplification of operation steps and reduction of the complicacy and uncertainty during the anaerobic culturing process; 2) reduction to dependency on the equipment requirements; 3) high accuracy and easiness in operation; 4) stable and reliably result, and good repeatability.

The invention provides a test strip for fast testing nitrate in food via the biochemical reduction method of nitrate reductase. System of the nitrate reductase + reduced coenzyme I (namely, nicotinamide adenine dinucleotide NADP) or system of the nitrate reductase + reduced coenzyme II (namely, nicotinamide adenine dinucleotide phosphate NADPH) catalyzes the nitrate to generate nitrite via the way of reduction. The test trip is applied to fast test nitrate content in food. The test trip is convenient in use, strong in specificity and beneficial to fast testing nitrate content in food.

The invention relates to a method for inhibiting generation of nitrite ion or nitrite in health care products, food or medicinal material containing nitrate ion or nitrate and nitrate reductase. The method comprises inhibiting the activity of nitrate reductase in the health care products, food or medicinal material by using the health care food, food or medically accepted nitrate reductase. The invention particularly relates to a method for inhibiting generation of nitrite ion or nitrite in cubilose, comprising using cubilose-accepted nitrate reductase inhibitor to inhibit the activity of the nitrate reductase in cubilose. Particularly, the method comprises adding the cubilose-accepted nitrate reductase inhibitor with effective inhibiting amount in newly collected cubilose or adding cubilose-accepted nitrate reductase inhibitor with the effective inhibiting amount in the cubilose production process. The nitrate reductase inhibitor can be selected from tungstate. By adopting the method, generation of nitrite ion or nitrite in cubilose can be effectively inhibited, and the edible risk of the cubilose can be lowered.

The invention discloses isoptericola and a method for using the isoptericola for synthesizing nano silver. The strain is Isoptericola sp. SYSUZL-3, preserved in China Center for Type Culture Collection on January 5, 2017 under the accession number of CCTCC NO:2017006. The strain has the ability to produce nitrate reductase to reduce silver ions for further synthesizing the nano silver. The strainSYSUZL-3 is activated, cultured, and centrifuged to obtain supernatant. A silver nitrate solution is added to the supernatant, and the nano silver is obtained by light irradiation. The nano silver hassmall particle size and high yield, and has obvious inhibitory effect on the growth of pathogenic bacteria such as Staphylococcus warneri, and sunlight is used to catalyze the reaction, and the synthesis efficiency is greatly improved. The synthesis method is low in production cost, simple in operation, non-pollution to the environment, and green, and has a good application prospect.

The invention relates to a method for reducing nitrate into nitrite wherein a probiotic and/or starter bacteriumis cultivated under anaerobic conditions in the presence of a nitrate, a heme and optionally a vitamin K.

The invention relates to a method for converting nitrates to nitrites and to specific compositions of bacteria belonging to the Staphylococcus vitulinus species optionally in association with lactic acid bacteria such as Lactococcus genus or Pediococcus genus and their use for developing the red color of a food product containing myoglobin.

This invention relates to agricultural fertilizers, mainly boron-molybdenum special fertilizers for peanut growth and their preparation method. Lack of boron or molybdenum may cause abnormal growth of peanuts, increase of empty peanut grains and quality declination. This invention provides a kind of boron-molybdenum special fertilizer for peanut cultivation, which comprises two components. Component 1 includes urea, potassium dihydrophosphate, potassium chloride, borax, ammonium molybdate, acetyisalicylic acid and triacontanol. Component 2 includes sodium alginate, xanthan gum and quicklime. The boron-molybdenum peanut special fertilizer is intended for southern earth which is seriously short of boron and molybdenum. According to many years' experience, by fertilized with this kind of fertilizer, activity of nitrate reductase and nodule nitrogenase in peanut leaves can be significantly promoted, nitrogen absorption, transportation and distribution can be enhanced, chlorophyll content and photosynthesis intensity in peanut leaves can be raised, peanut weight can be increased and both the quality and the yield can be promoted. The peanut yield can be increased by 10~18% per unit of area.

The invention discloses an immobilized bacteria combined multi-component solid carbon source pellet and a preparation method thereof. The preparation method includes the following steps: (1) pretreating husks; (2) preparing a polyvinyl alcohol-sodium alginate-husk mixture; (3) adding manganese chloride and growth factors or the manganese chloride, sodium molybdate and the growth factors to the polyvinyl alcohol-sodium alginate-husk mixture to obtain a multi-component mixture; and (4) adding a bacterial suspension containing denitrifying bacteria to the multi-component mixture, and extruding the mixture of the bacterial suspension and the multi-component mixture to a CaCl2 solution for a cross-linking reaction to obtain the immobilized bacteria combined multi-component solid carbon source pellet. The immobilized bacteria combined multi-component solid carbon source pellet of the invention can provide a stable carbon source for low carbon-nitrogen ratio sewage, can realize a efficient and stable denitrification process, and has the advantages of maintaining the activity of immobilized bacteria for a long time, promoting the formation of a nitrate reductase and enhancing the nitrogenremoval efficiency of the immobilized bacteria.

The invention provides application of lactobacillus casei in preparation of fermented sausages, a method for preparing sausages and sausages. The lactobacillus casei is collected in General Microbiological Center of China Committee for Culture Collection of Microorganisms on June 2nd, 2017, and the collection number is CGMCC No.14212. The lactobacillus casei does not generate viscosity, gas, ammonia, H2O2 and H2S, but generates nitrate reductase; by addition of the lactobacillus casei, the accumulation of other seven biogenic amines except 2-phenethylamine in the sausages can be suppressed, especially for histamine with largest toxicity, the control effect is most obvious. In addition, by addition of the lactobacillus casei, the effects of obviously increasing the quantity of lactic acid bacteria in the fermented sausages, suppressing growth of escherichia and staphylococcus, and increasing the brightness value and the redness value of the fermented sausages also can be achieved, the sausages are endowed with softer and more elastic structures so as to be received by consumers more easily.

The invention discloses a method for promoting nitrate reduction in soil. According to the method for promoting the nitrate reduction in the soil, research shows that a potential gradient is generatedfrom a cathode to an anode after voltage treatment, so that reducing microorganisms of nitrate are enriched and the process of dissimilatory reducing of the nitrate to ammonium and denitrification isstrengthened significantly, and end products are NH4<+> and N2. The increased nitrogen content in the form of N2O, N2 and ammonium is equivalent to the content of nitrogen lost by nitrate under the voltage treatment. The results demonstrate that electrical potential can enhance the nitrate reduction in the soil through denitrification and DNRA. The invention provides a targeted and environment-friendly technology to enhance the nitrate reduction of microorganisms under the roots of crops, thereby reducing the risk of nitrate leaching.

The invention discloses a method for producing an organic water-soluble fertilizer by utilizing straws. After compound bacteria are adopted for fermentation, compound enzymes are used for enzymolysis,the production technological process is optimized, the nitrogen, phosphorus and potassium nutrient content of the product reaches 300 g/L, organic matter content reaches 100 g/L, the fertilizer product is an alkaline fertilizer, and the product has the advantages of being fully water-soluble, high in activity, fully absorbed, quick in effect and high in water-soluble potassium content. The fertilizer is suitable for protected soil subjected to long-term fertilization and acidification, solves the problem of severe salt accumulation of greenhouse soil, can improve soil and increase the organicmatter content of soil, and effectively relieves the symptoms of soil acidification, hardening, fertility decline, nutrition imbalance and the like. After the product is applied, beneficial microbialflora can be activated, the activity of nitrate reductase is promoted, and amino acid and protein synthesis is accelerated; the water-soluble organic matters have strong permeability, promote protoplast flow of crops and plants, and accelerate nutrient absorption of root systems; and high-activity organic matters and medium trace element crops are directly absorbed without conversion so that thenutrition is comprehensively supplemented, and the effect is quick.

The invention relates to clone and application of a loose leaved Chinese cabbage nitratase gene BcNR, belonging to the molecular biology and biotechnology field. Total RNA is extracted from a loose leaved Chinese cabbage leaf and then 2 gamma of the total RNA is reversely transcribed into cDNA; three pairs of special primers are designed according to conservative nucleotide sequences in an NIAI (mouseearcress) and an NR (cabbage type rape) for routine polymerase chain reaction (PCR); PCR products are respectively connected to pMD18-T vectors; DH5alpha cells are transformed and sequencing is performed; full-length cDNA is obtained through quick amplification of 3' terminals and 5' terminals; a sense expression vector is further constructed and the NIAI is transformed. The transgenic NIAI has high nitratase transcription level. The genetic transformation vegetable can adjust the content of nitrate in the vegetable and improve the output and the quality and have very important economic benefit and social benefit.