30 results about "Staphylococcus simulans" patented technology

Monoclonal antibodies which can bind to the ClfA protein and which are generated from binding subdomains or active fragments of the ClfA protein from Staphylococcus aureus, including the active fragments proteins from its fibrinogen binding domain such as Clf40 protein, the Clf33 protein, or ClfA N3, are provided which can be useful in the treatment and protection against infection from staphylococcal bacteria such as Staphylococcus aureus. In addition, medical instruments can be treated using the monoclonal antibodies of the invention in order to reduce or eliminate the possibility of their becoming infected or further spreading the infection. In particular, the antibodies of the present invention are advantageous because they can prevent adherence of the bacteria to host cells by impairing or inhibiting the ability of S. aureus ClfA to bind to fibrinogen or fibrin, and thus can be utilized in methods or treating or preventing staphylococcal inventions.

The invention discloses a high density fermentation technology of staphylococcus simulans L-RG18 having good fermentation characteristics and separated from France dry sausage, the high density fermentation technology is a high density culture model comprising optimal control of a fermentation culture medium and fermentation process conditions and repeated in-batch coupling cell cycle, according to the high density fermentation technology, a high density fermentation liquid with the living bacterium number of 3.72*1011 CFU / mL is obtained, and the high density fermentation technology lays a foundation for further studies on preparation of direct-adding type staphylococcus simulans meat starter cultures.

The invention discloses an East Asian scorpion antimicrobial peptide gene and its preparation method and application, which is characterized in that the scorpion venom antibacterial peptide is recombinant Escherichia coli DH5a / BmKAMP1, CCTCC NO: M207036, and the steps are as follows: firstly, the East Asian scorpion venom is constructed Gland cell cDNA library; secondly, the positive clones of scorpion antimicrobial peptide gene were screened from poison gland cDNA library by PCR method; the coding characteristics of the antimicrobial peptide gene were sequence analyzed to determine the amino acid sequence of the antimicrobial peptide; Lambert bacteria have inhibitory effects at different concentrations. In particular, the East Asian scorpion antimicrobial peptide has a strong inhibitory effect on drug-resistant hemolytic staphylococcus, and has a good antibacterial effect. The antimicrobial peptide of the scorpion scorpion of the invention is specific to Gram bacteria, has high activity and is simple and convenient. Can be used as antibacterial drug application.

The invention relates to Staphylococcus epidermidis CGMCC 3472 and application thereof in producing fermented segmental pork. The mouthfeel and other qualities of pork blocks of the fermented segmental pork produced by the strain approach or are superior to those of naturally-fermented hams; in addition, the fermented segmental pork has short production period and can be manufactured into small packages; meanwhile, the quality stability of products can be improved.

The invention discloses a preparation technology of cantonese style sausage, belonging to field of food processing. The preparation technology of the cantonese style sausage comprises the steps of a. preparation of zymophyte, wherein the zymophyte comprises lactobacillus pentosus, pediococcus acidilactici, staphylococcus xylosus, staphylococcus simulans, micrococcus uarians and staphylococcus epidermidis; respectively culturing strains of the zymophyte for later use; b. preparation of raw materials; c. preparation of ingredients; d. pickling, namely, evenly mixing the raw materials and the ingredients, and then inoculating the strains of the zymophyte in sequence; and e. filling the sausage, and drying to obtain the finished product. After the preparation technology of the cantonese style sausage is adopted, the production time of the cantonese style sausage is greatly shortened, and the yield of the cantonese style sausage is increased; and the specially-made ingredients are adopted, so that the cantonese style sausage prepared by the method is better in flavor compared with the cantonese style sausage prepared by the traditional method.

The invention belongs to the field of microbiological detection, and in particular relates to a method for detecting staphylococcus aureus and two monoclonal antibodies of the staphylococcus aureus which are produced by the same monoclonal antibody preparation process. The monoclonal antibodies can be specifically combined with the staphylococcus aureus. The monoclonal antibodies are generated by mouse hybridoma cell lines 5D2G2D9C1, CGMCCNo.8763 or 5D6D9G3B4, CGMCCNo.8764. The invention also provides application of the monoclonal antibodies for resisting the staphylococcus aureus.