5 results about "Staphylococcus simulans" patented technology

The present invention provides novel systems, methods, and compositions for making and using novel engineered Cas9 enzymes that are optimized for human cells for nucleic acid targeting and manipulation. The present invention relates to codon-optimized and recombinantly produced Streptococcus equinus ATCC 33317, Enterococcus hirae strain F1129E, Streptococcus equinus strain AG46, Staphylococcus simulans strain 19, Streptococcus intermedius B196 strain G1552, Streptococcus sanguinis SK330, Streptococcus sp. C150, Streptococcus oralis subsp. oralis strain RH_1735_08, Streptococcus oralis SK313, Staphylococcus warneri strain 691, Staphylococcus stiuri strain SNUC 2430, Streptococcus gallolyticus strain AM24-4, Lactobacillus kullabergensis strain Biut2, and Streptococcus The present invention is based on the discovery of a novel Cas9 enzyme from the bacterium suis strain LSS83. In some embodiments, the novel Cas9 enzyme can be used for base editing. In some embodiments, the novel engineered Cas9 enzyme is used to treat human diseases.

The present invention relates to the technical field of microbial food fermentation, and specifically discloses the application of Staphylococcus simulans HZ01 to replace part of nitrite in meat products. The detection of the concentration of nitrite includes the following steps: (i) preparing an NB medium containing an appropriate concentration of KNO3 / NaNO3; (ii) inoculating the Staphylococcus simulans HZ01 bacterial solution into the NB medium in step (i) for cultivation; (iii) centrifuging the fermentation broth at intervals during the cultivation process and collecting the supernatant; (iv) detecting the nitrite content in the supernatant of step (iii). The present invention has the characteristics of good processing adaptability, relatively high protease and lipase activities, good nitrate reductase activity, and better flavor of the fermented meat products.

The present application relates to the technical field of microbial food fermentation, and specifically discloses a Staphylococcus simulans HZ01 inoculant, preparation of the Staphylococcus simulans HZ01 inoculant comprising the following steps: (A) inoculating Staphylococcus simulans HZ01 into NB culture medium for culture to obtain a bacterial solution; (B) when the concentration of Staphylococcus simulans HZ01 in the bacterial solution obtained in step (A) is 1.0×10 8 log 10 CFU / mL to 9.9×10 9 log 10 CFU / mL, centrifugation is performed; (C) after completion of the centrifugation, sterilized skimmed milk is added for resuspension; (D) after completion of the resuspension, freeze-drying is performed to obtain a powdered Staphylococcus simulans HZ01 inoculant with a concentration of 1.0×10 10 log 10 CFU / g to 9.9×10 11 log 10 CFU / g. The present application has the characteristics of good processing adaptability, high protease and lipase activity, good nitrate reductase activity, and good flavor of fermented meat products.

The invention discloses a high-strength mineralized strain as well as a culture method and application thereof, the high-strength mineralized strain is classified and named as Staphylococcus simulans ZN1 #, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has the preservation number of CGMCC No.32123. The high-strength mineralized strain has the advantages that the high-strength mineralized strain is high-strength mineralized strain; the optimal culture conditions are as follows: the culture temperature is 30 DEG C, the culture rotating speed is 200 r / min, and the culture time is 24 hours. The strain has high soil mineralization strength and can be widely applied to microbial mineralization and reinforcement of soil. The strain has the characteristics of small thallus size and small crystal structure of calcite generated by induction, is especially suitable for mineralization and reinforcement of fine-particle soil, has high strength and good stability in fine-particle soil solidification, and solves the bottleneck problem of difficult application of a microbe-induced mineralization technology in fine-particle soil.

The present invention relates to the technical field of microbial food fermentation, and specifically discloses the application of a Staphylococcus simulans HZ01 in dry-cured fermented meat products. The preparation of the dry-cured fermented meat products includes the following steps: (1) taking the concentrated solution or powder of Staphylococcus simulans HZ01 and diluting it with edible water; (2) dissolving appropriate amounts of table salt, white sugar, and monosodium glutamate in the solution in step (1) to prepare a pickling solution; (3) taking minced meat, adding the pickling solution in step (2) to the minced meat, and when the concentration of Staphylococcus simulans HZ01 in the minced meat reaches 1.0×10 7 log 10 CFU / g to 9.9×10 8 log 10 CFU / g, performing low-temperature pickling on the minced meat; (4) after the pickling is completed, subjecting the minced meat to enema, exhaust, baking, and fermentation in sequence to obtain the finished dry-cured fermented meat products. The present invention has the characteristics of good processing adaptability, relatively high protease and lipase activities, good nitrate reductase activity, and better flavor of the fermented meat products.