The present application relates to the technical field of microbial food
fermentation, and specifically discloses a
Staphylococcus simulans HZ01 inoculant, preparation of the
Staphylococcus simulans HZ01 inoculant comprising the following steps: (A) inoculating
Staphylococcus simulans HZ01 into NB culture medium for culture to obtain a bacterial solution; (B) when the concentration of
Staphylococcus simulans HZ01 in the bacterial solution obtained in step (A) is 1.0×10 8 log 10 CFU / mL to 9.9×10 9 log 10 CFU / mL,
centrifugation is performed; (C) after completion of the
centrifugation, sterilized
skimmed milk is added for resuspension; (D) after completion of the resuspension, freeze-
drying is performed to obtain a powdered
Staphylococcus simulans HZ01 inoculant with a concentration of 1.0×10 10 log 10 CFU / g to 9.9×10 11 log 10 CFU / g. The present application has the characteristics of good
processing adaptability, high
protease and
lipase activity, good
nitrate reductase activity, and good
flavor of fermented meat products.