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Staphylococcus simulans L-RG18 fermentation medium for use and high density culture method

A L-RG18, imitating Staphylococcus technology, applied in meat starter to imitate Staphylococcus L-RG18 fermentation medium, high-density culture field, to avoid repeated cleaning, improve production efficiency, and reduce separation difficulty.

Active Publication Date: 2014-04-09
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of membrane cell circulation coupled with repeated batch fermentation can combine the advantages of the two fermentation methods, increase the cell density of the fermenter, shorten the production cycle, and reduce the difficulty of downstream cell separation, thereby improving production efficiency. Density culture has not been reported

Method used

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  • Staphylococcus simulans L-RG18 fermentation medium for use and high density culture method
  • Staphylococcus simulans L-RG18 fermentation medium for use and high density culture method
  • Staphylococcus simulans L-RG18 fermentation medium for use and high density culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Imitation Staphylococcus L-RG18 fermentation medium optimization

[0051] Bacterial strain activation: inoculate the imitation Staphylococcus L-RG18 bacterium powder that cryopreservation is in the MSA medium test tube, 30 ℃ of shaker cultures 18h, shaker speed is 140rpm / min, with 1% inoculum amount continuous activation three generations in the test tube, Make the number of viable bacteria reach 10 7 -10 8 CFU / mL is the activated strain.

[0052] 1. Screening of basal medium

[0053] Five different liquid basal media were investigated: TSB, MSA, Nutrient Broth, M17, Growth Medium. The growth medium formula is: casein peptone 10g / L, beef extract 1g / L, glucose 5g / L, sodium chloride 5g / L, dipotassium hydrogen phosphate 3g / L, and adjust the pH value to 7.4 during preparation. The activated Staphylococcus imitatus L-RG18 was inoculated in 5 kinds of media with 1% inoculation amount, the shaking table speed was 140rpm / min, and the culture temperature was 30°C. ...

Embodiment 2

[0068] Example 2 Imitation Staphylococcus L-RG18 fermentation condition optimization

[0069] 1. Determination of the optimum pH

[0070] The activated Staphylococcus mimetic L-RG18 was inoculated with 1% inoculum into the optimized fermentation medium with pH values ​​of 7.0, 7.2, 7.4, and 7.6, cultured at 30°C, 140r / min shaking for 18 hours, and the fermentation under different conditions was determined. The number of liquid viable bacteria. Test results such as Figure 8 It was shown that the initial pH value of the medium had a greater impact on the growth of the strain, and the enrichment effect was significantly higher when the initial pH value of the medium was 7.4 than in other groups (P<0.05). Too high or too low a pH value has a certain inhibitory effect on the growth of the strain, so the initial pH value of the imitation Staphylococcus L-RG18 medium is 7.4.

[0071] 2. Determination of the optimum temperature

[0072] Inoculate the activated Staphylococcus imit...

Embodiment 3

[0075] Example 3 Imitation of high-density culture of Staphylococcus L-RG18

[0076] 1. Preparation of seed culture solution: Inoculate the frozen-preserved imitative Staphylococcus L-RG18 bacteria powder in a test tube of fermentation medium, culture at 30°C and 200r / min for 18 hours, and continuously activate three generations in the test tube with 1% inoculation amount, and then Inoculate 1% of the inoculum into a Erlenmeyer flask (500 mL) filled with 150 mL of fermentation medium, and culture at 30 °C and 200 r / min for 18 hours to obtain a seed culture solution.

[0077] 2. Determine the high-density culture method

[0078] (1) Batch culture in 5L fermenter

[0079]The seed culture solution was inoculated into a 5L fermenter with a 1% inoculum volume, the liquid volume was 3L, the culture temperature was 30°C, the stirring speed was 200r / min, and the flow rate of sterile air was 1.7NL / min. The fermentation time was 24 hours, and samples were taken at regular intervals du...

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Abstract

The invention discloses a high density fermentation technology of staphylococcus simulans L-RG18 having good fermentation characteristics and separated from France dry sausage, the high density fermentation technology is a high density culture model comprising optimal control of a fermentation culture medium and fermentation process conditions and repeated in-batch coupling cell cycle, according to the high density fermentation technology, a high density fermentation liquid with the living bacterium number of 3.72*1011 CFU / mL is obtained, and the high density fermentation technology lays a foundation for further studies on preparation of direct-adding type staphylococcus simulans meat starter cultures.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a meat starter imitating a staphylococcus L-RG18 fermentation medium, culture conditions and a high-density culture method. Background technique [0002] Staphylococcus is an important class of microorganisms in the production of fermented meat products. During the fermentation process of meat products, it can produce nitrate and nitrite degrading enzymes, so that the meat has a bright rose red color, and some staphylococci can also produce H 2 o 2 Enzymes are not only beneficial to the color protection of fermented sausages, but also play an important role in inhibiting spoilage. In addition, they produce enzymes that degrade proteins and fats, and the enzymatic hydrolysis products play an important role in the development of the characteristic flavor of dry fermented sausages. Screening staphylococci with excellent fermentation characteristics, and preparing t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/44
Inventor 李平兰桂萌张晓琼马长伟王洋
Owner CHINA AGRI UNIV
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