Preparation method of synbiotic goat milk powder
A technology for goat milk powder and milk powder, which is applied in the fields of milk preparations, dairy products, and applications to achieve the effect of high viable bacteria count and improved survival rate.
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Embodiment 1
[0026] First: firstly activate the probiotic bacteria to make the probiotic bacteria mud, then add the same weight of composite freeze-drying protection agent to the probiotic bacteria mud, mix well and pre-freeze at -40 ℃ for 8 hours, and then move it to the freeze dryer Freeze-drying for 20-24h to obtain freeze-dried probiotic bacteria powder;
[0027] Wherein, the composite freeze-drying protective agent is composed of 30% skimmed milk powder, 20% trehalose, 2% glutamic acid, 1% glycerol and 47% pH 6.5 citric acid buffer by mass percentage;
[0028] The preparation of probiotic bacteria mud is to make the number of viable bacteria of the bacteria powder as 1.0×10 10 -2.0×10 11 cfu / g Lactobacillus acidophilus was activated in MRS liquid medium, and the microscopic examination and OD value were determined to confirm that there were no impurities. After the OD value was >0.5, it was activated for three times, and then it was connected to a 10L fermenter and cultivated at a co...
Embodiment 2
[0035] First: firstly activate the probiotic bacteria to make the probiotic bacteria mud, then add the same weight of composite freeze-drying protection agent to the probiotic bacteria mud, mix well and pre-freeze at -40 ℃ for 8 hours, and then move it to the freeze dryer Freeze-drying for 20-24h to obtain freeze-dried probiotic bacteria powder;
[0036]Wherein, the composite freeze-drying protective agent is composed of 70% skimmed milk powder, 8% trehalose, 1% glutamic acid, 1% glycerol and 20% citric acid buffer with a pH of 6.5 by mass percentage;
[0037] The preparation of probiotic bacteria mud is to make the number of viable bacteria of the bacteria powder as 1.0×10 10 -5.5×10 10 The viable count of Lactobacillus casei and bacterial powder of cfu / g is 1.0×10 10 -3.4×10 10 Lactobacillus plantarum with cfu / g was activated in MRS liquid medium, and the microscopic examination and OD value were determined to confirm that there were no impurities. After the OD value was ...
Embodiment 3
[0044] First: firstly activate the probiotic bacteria to make the probiotic bacteria mud, then add the same weight of composite freeze-drying protection agent to the probiotic bacteria mud, mix well and pre-freeze at -40 ℃ for 8 hours, and then move it to the freeze dryer Freeze-drying for 20-24h to obtain freeze-dried probiotic bacteria powder;
[0045] Wherein, the composite freeze-drying protective agent is composed of 50% skimmed milk powder, 10% trehalose, 1.5% glutamic acid, 1.5% glycerol and 37% citric acid buffer with pH 6.5 by mass percentage;
[0046] The preparation of probiotic bacteria mud is to make the number of viable bacteria of the bacteria powder as 1.0×10 10 -5.6×10 10 The viable count of Lactobacillus rhamnosus and bacterial powder in cfu / g is 1.0×10 10 -5.2×10 10 The viable count of Lactobacillus reuteri and bacterial powder in cfu / g is 1.0×10 10 -4.0×10 10 cfu / g Lactobacillus bulgaricus was activated in MRS liquid medium, and microscopic examination...
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