Method for strengthening indigenous microbes and improving oil recovery by improving microbial florae in oil deposit

A technology of microbial flora and original microorganisms, which is applied in the direction of mining fluid, earthwork drilling, wellbore/well components, etc., can solve the problems of microbial flora structure differences, application limitations, lack of functional flora, etc., and expand the scope of application , Improve the effect of oil displacement, the effect of function strengthening

Inactive Publication Date: 2010-11-24
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, due to the difference in geological conditions, the environmental conditions of each oil reservoir are also different, resulting in great differences in the structure of the microbial flora in the oil reservoirs. Some oil reservo

Method used

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  • Method for strengthening indigenous microbes and improving oil recovery by improving microbial florae in oil deposit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Molecular Ecological Analysis of Oil Reservoir Microflora Composition

[0031] The samples were taken from the produced water of the oil well, and were filtered step by step using filter membranes with pore sizes from large to small, with a 0.22 μm filter membrane used for the last suction filtration. The microbial cells on the filter were collected, and the total DNA was extracted with a DNA extraction kit (UltraCleanTM Soil DNA Kit). The extracted total DNA was detected by 0.8% agarose gel electrophoresis and stored at -20°C.

[0032] Take 1 μl of total environmental DNA as a template for PCR, and the primers are:

[0033] bacteria:

[0034] 27F: 5'AGAGTTTGATC2CTGGCTCAG-3',

[0035] 1495R: 5'-CTACGGCTACCTTGTTACG-3');

[0036] Archaea:

[0037] 21F: 5'TCCGGTTGATCCTGCCGGA-3',

[0038] 1495R: 5'-CTACGGCTAC CTTGTTACG-3').

[0039] Amplification conditions: 94°C for 4min; 30 cycles of 94°C for 30s, 50°C for 30s, 72°C for 60s; 72°C for 7min. The yield and specificity...

Embodiment 2

[0042] Embodiment 2, the culture analysis and selection of microbial functional flora

[0043] The dilution method was used to analyze and enrich the number of bacteria of different physiological flora in the oil reservoir samples, and the culture medium used was as follows:

[0044] Culture medium for hydrocarbon oxidizing bacteria:

[0045] Ammonium nitrate 1.5g / l, urea 0.5g / l, dipotassium hydrogen phosphate 1.0g / l, potassium dihydrogen phosphate 1.0g / l, calcium chloride 0.01g / l, magnesium sulfate 0.5g / l, ferrous sulfate 0.01 g / l, 2% liquid wax, pH7.0;

[0046] Culture medium for heterotrophic bacteria:

[0047] Peptone 10g / l, yeast extract 3g / l, glucose 10g / l, sodium chloride 3g / l, pH7.0;

[0048] Sulfate-reducing bacteria culture medium:

[0049] Sodium Lactate 4g / l, Ammonium Sulfate 4g / l, Disodium Hydrogen Phosphate 3.5g / l, Potassium Dihydrogen Phosphate 1.5g / l, Magnesium Sulfate 1g / l, Calcium Chloride 0.1g / l, Yeast Extract 1.0g / l, Cysteine ​​hydrochloride 1g / l, Mohr...

Embodiment 3

[0060] Analysis of metabolic characteristics of microbial flora in oil reservoir samples

[0061] Add 100ml of formation water sample to the 250ml serum bottle, and add crude oil and other nutrients for cultivation. During the experiment, indicators such as crude oil emulsification, pH, surface tension and methane gas changes were detected, and hydrocarbon oxidizing bacteria, fermenting bacteria, saprophytic bacteria, methane bacteria and sulfate reducing bacteria were counted at the same time.

[0062] The results show that the crude oil of S12-4 undergoes obvious emulsification, the surface tension and pH value decrease, and a certain amount of gas is produced. These characteristics can facilitate the release of crude oil from the reservoir. And station 3-X27 effect is relatively poor.

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Abstract

The invention provides a method for strengthening indigenous microbes and improving oil recovery by improving microbial florae in an oil deposit. The method comprises the following steps of: (1) analyzing composition of microbial florae in the oil deposit; and (2) introducing exogenous microbes into the oil deposit according to an analysis result, so that the oil deposit with the introduced exogenous microbes contains the following microbes: hydrocarbon-oxiding bacteria, fermentative bacteria, heterotrophic bacteria, methanogenic bacteria, sulphate-reducing bacteria, and nitrate-reducing bacteria. Due to the adoption of the method provided by the invention, by adding the exogenous microbes into the oil deposit, the structure of the microbial florae in the oil deposit is improved, the function of the microbial florae in the oil deposit is strengthened, as a result, the oil displacement effect is improved, and the application range of microbial oil recovery technology is enlarged.

Description

technical field [0001] The invention relates to a method for improving microbial flora in oil reservoirs to strengthen native microorganisms and improve oil recovery. Background technique [0002] Oil is an important resource, and people's demand for oil is increasing day by day. However, the existing oil extraction technology can only extract about 1 / 3-1 / 2 of the reserves, and the remaining recoverable oil reserves are huge. How to extract these oil resources is a problem that people pay close attention to. [0003] Microbial oil recovery technology (MEOR) is a biotechnology that improves crude oil production and recovery by utilizing the life activities of microorganisms in oil reservoirs and their metabolites. Microbial oil recovery technology has a wide range of applications, low implementation costs and significant economic benefits. And other characteristics, more and more people's attention. [0004] According to different sources of microorganisms, MEOR is divided ...

Claims

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Application Information

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IPC IPC(8): E21B43/22
Inventor 薛燕芬马延和马树恒袁三青
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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