19 results about "Flagellum" patented technology

The application discloses an oral nano-vaccine and a preparation method and application thereof, and relates to the technical field of immunology, and specifically discloses an oral nano-vaccine which comprises a nano-particle wrapped by a biomimetic bacterial membrane vesicle and an outer layer; the nano-particle comprises an antigen and a biodegradable polymer material; the biomimetic bacterial membrane vesicle comprises an ionizable flagellar lipoprotein, an immune adjuvant, a phospholipid, a sterol lipid and a polyethylene glycol lipid; and the outer layer is a pH-responsive polymer. The oral nano-vaccine provided by the application combines the immune activation advantages of natural bacterial membrane vesicles and the precise regulation characteristics of synthetic materials, significantly improves the transmembrane penetration capacity of the antigen in the intestinal epithelium and the overall activation effect of the mucosal immune system. The oral nano-vaccine provided by the application can protect the antigen and the immune adjuvant from degradation and realize precise release in the intestinal microenvironment; and the oral nano-vaccine significantly improves the bioavailability and safety of an oral tumor vaccine, and lays a key technical foundation for clinical transformation and large-scale production of the oral tumor vaccine.

The application provides a flexible flagellum-driven swimming micro-nano robot and a control method thereof, and relates to the technical field of micro-nano robots.The robot comprises a head and a flexible flagellum, the inside of the head is provided with a control component, a monitoring component, a driving component and a power source, the driving component is connected with the flexible flagellum, and the method comprises the following steps: a control chip reads chemical concentration or light field intensity information detected by an environmental sensor; the collected chemical concentration or light field intensity information is compared with historical time information; the driving frequency of a micro-actuator is switched according to the comparison result; and the swimming micro-nano robot is driven to swim.The application fully utilizes the complex fluid-structure coupling behavior between the flexible flagellum and the fluid, does not need a complex steering mechanism, only needs simple information storage and logical judgment to change the swing frequency of the flagellum, and can realize the autonomous navigation movement of the micro-nano robot in a complex environment, and gradually approaches the target along a variable-parameter spiral trajectory.

The application discloses a kind of biological hybrid micro machine population oral delivery system of imitating Helicobacter pylori survival mechanism, belong to oral stomach medicine delivery technical field.The system is by active power module (flagellum driven chlamydomonas reinhardtii), environmental regulation module (acid urease modified on algal surface) and multifunctional load module (macrophage membrane wrapped drug-loaded nanoparticles) synergistic assembly through polydopamine (PDA) spatial conformation control layer is formed.Active power module utilizes self-movement ability to penetrate mucus barrier and extend intracavity retention time to 12 hours or more;Environmental regulation module produces local alkalization effect by catalyzing urea hydrolysis, provides chemical shield for system in extremely acid environment of pH 1.0-3.0, protects active power module from gastric acid inactivation;Multifunctional load module is accurately delivered to gastric epithelial cells by inflammation targeting effect, such as CRISPR / Cas9 biological macromolecule, and realizes up to 31.8% in-vivo gene editing efficiency.The application provides a non-invasive, modular active delivery platform for the precise treatment of gastric diseases such as gastritis through the synergistic effect of physical penetration, metabolic reprogramming and gene intervention.

The invention provides application of piceatannol in preparation of a salmonella flagellum inhibitor, and belongs to the technical field of biological medicines. Through analysis of salmonella sliding motion test, biofilm formation test, salmonella cell invasion test and other tests, it is found that piceatannol down-regulates flagellum-related gene transcription, weakens the sliding capacity, reduces biofilm formation and weakens the invasion capacity to host cells at non-inhibitory concentration; the invention relates to a salmonella flagellum inhibitor, particularly relates to a salmonella flagellum inhibitor, supports the development value of the salmonella flagellum inhibitor as an anti-virulence drug, provides a non-bactericidal treatment strategy capable of remarkably weakening bacterial pathogenicity, can be used as a potential lead compound or a composition therapy component for treating salmonella infection, and is applied to production and preparation of the salmonella flagellum inhibitor.

The application provides an application of a grass Wu extract, relates to the field of bacteriostatic technology, and is used for inhibiting growth of escherichia coli; the minimum bacteriostatic concentration of the grass Wu extract is 2.5 mg / mL; the grass Wu extract can directly destroy the cell wall and the cell membrane of bacteria, down-regulate key genes of a flagellum assembly path, interfere with a core metabolic path of bacteria, and down-regulate related genes such as a two-component system and ABC transporter proteins; the MIC of the grass Wu extract on escherichia coli is determined as 2.5 mg / mL for the first time, which provides an accurate dose basis for developing the grass Wu extract into an antibacterial preparation, proves that the grass Wu extract has a definite and quantifiable direct bacteriostatic effect; meanwhile, through a multi-target attack strategy of 'breaking a membrane'-'breaking a leg'-'breaking food'-'causing blindness', the bacteria are difficult to produce drug resistance through a single mutation, and a new idea is provided for solving the problem of antibiotic drug resistance.

The invention belongs to the field of biology, and discloses a proteus mirabilis homologous recombination system expression plasmid which comprises a pBR322 replication starting point, a resistance gene, an arabinose inducible promoter and a proteus mirabilis-derived homologous recombination operon. The homologous recombination operon is used for coding a TPM66325 protein, an EPM66325 protein, a single-chain binding protein SSB and a fake release protein. The expression plasmid has relatively high recombination efficiency in proteus mirabilis GDMCC 66325 and can also play a recombination role in a proteus mirabilis standard strain ATCC35659, and the expression plasmid is obtained by knocking out a related gene bcsB formed by a hemolysin related gene hpmA and a biological membrane of the proteus mirabilis GDMCC 66325 and directly knocking in a heterologous gene after a related gene cluster is adjusted by flagellum. The effectiveness of the expression plasmid in recombinant operation of proteus mirabilis is verified; meanwhile, the invention further discloses a proteus mirabilis homologous recombination system, application and a gene knockout / knockin method.

This invention discloses the pharmaceutical applications of a P2Y11-specific inhibitor and compositions containing it, belonging to the field of biomedical technology. This invention discloses for the first time the use of NF157 in the preparation of drugs related to the treatment of HD5-induced pathogen infection. In combating pathogen infection, NF157 can specifically inhibit the binding of P2Y11 to HD5, thereby eliminating the role of HD5 in regulating cytoskeleton rearrangement and promoting filopodia formation, thus becoming a key link in interrupting pathogen infection. By preventing the formation of filopodia, NF157 effectively blocks the bacterial capture pathway, showing significant intervention effects on some pathogens that lack adhesion devices and flagella and rely on cellular pseudopodia capture for invasion.

The application discloses a kind of methods for measuring the movement direction of flagellum algae under flow conditions, the method comprises the following steps: step 1, image sequence acquisition and processing;Step 2, cell image cropping alignment;Step 3, cell image pre-processing;Step 4, create fan ring detection area;Step 5, construct timing frame;Step 6, time sequence difference feature calculation;Step 7, confidence calculation and movement direction determination.The method for measuring the movement direction of flagellum algae under flow conditions disclosed in the application overcomes the technical problems that cannot be identified due to poor imaging quality of flagellum and loss of instantaneous information in moving water environment, and realizes high-precision, automated and continuous measurement of the movement direction of flagellum algae through time sequence dynamic feature analysis and biological motion characteristic determination.

The application provides a kind of lipopeptide compound PW-1 and its preparation method and application in preventing and controlling bacterial wilt, belong to biological pesticide technical field;The PW-1 is prepared by fermentation, solvent extraction, acidification and reverse phase chromatography from Paenibacillus polymyxa (P. Paenibacillus polymyxa ) strain DW-7A;The core of the compound is that it plays a role through "anti-toxicity" mechanism, at the concentration without inhibiting the growth of Pseudomonas solanacearum (P. Ralstonia solanacearum ), can specifically interfere with the biosynthesis of pathogenic bacteria flagellum, cause its motility, chemotaxis and early infection ability to decrease significantly;Pot experiment proves that the relative control efficiency of PW-1 to tobacco bacterial wilt reaches 62.2%, and has no effect on the growth of soil bacillus at the concentration of up to 1000 μg / mL, shows efficient, targeted and environment compatible characteristics;The application provides a new candidate compound and key technical support for the development of green pesticide targeting flagellum.

The invention discloses a method for measuring the motion direction of flagellates under a flowing condition. The method comprises the following steps: step 1, acquiring and processing an image sequence; 2, cutting and aligning the cell image; step 3, preprocessing the cell image; step 4, creating a sector ring detection area; step 5, constructing a time sequence frame; step 6, calculating time sequence difference characteristics; and 7, calculating confidence and determining a motion direction. According to the method for measuring the motion direction of the flagellum under the flowing condition, the technical problem that the flagellum cannot be identified due to poor flagellum imaging quality and instantaneous information loss in a flowing water environment is solved, and high-precision, automatic and continuous measurement of the motion direction of the flagellum is realized through time sequence dynamic characteristic analysis and biological motion characteristic judgment.

The application discloses application of amino peptidase SapM in regulating virulence and motility of Xanthomonas campestris, and belongs to the technical field of biotechnology.The sapM gene function is researched by constructing a sapM gene deletion strain of Xanthomonas campestris.The research finds that knocking out the sapM gene (Delta sapM) significantly enhances the pathogenicity, extracellular polysaccharide (EPS) synthesis, amylase activity and motility of Xcc, while sapM overexpression inhibits the above-mentioned phenotypes.The flagellum morphology of the sapM mutant strain is further observed by transmission electron microscopy, and it is found that the flagellum length of the sapM mutant strain is significantly different from that of the wild type.It can be seen that the amino peptidase SapM is involved in negative regulation of virulence and motility of Xanthomonas campestris, and the application has great application value.

According to the Escherichia coli engineering strain with enhanced athletic ability and the construction method and application thereof, wild Escherichia coli MG1655 is taken as a transformation starting point, and expression of flagellum power regulation protein CheZ is specifically enhanced, so that the chemotactic athletic ability of the strain is remarkably enhanced, the migration range of the transformed strain on a soft agar plate is expanded compared with that of the wild Escherichia coli MG1655, and the activity of the transformed strain on the soft agar plate is improved. And the rapid growth characteristic of the original host is maintained. The strain can break through the space motion limitation of traditional microorganisms in soil remediation, achieves targeted migration of deep soil pollutants, and is suitable for efficient bioremediation of heavy metal and organic pollutants in a heterogeneous soil environment.

The invention belongs to the technical field of microorganisms and genetic engineering. The invention provides a listeria monocytogenes strain as well as a preparation method and application thereof, the flagellum formation ability of the strain is enhanced, the environmental stress resistance is enhanced, and the immune recognition of host cells on listeria monocytogenes can be enhanced; the strain is subjected to delta yxeA gene deletion mutation; the bacterial strain is named as Listeria monocytogenes Lm-delta lmo2568, the preservation number of the bacterial strain is CGMCC (China General Microbiological Culture Collection Center) No.36001, and the bacterial strain is preserved in the China General Microbiological Culture Collection Center on September 22, 2025. The invention finds that the deletion of Listeria monocytogenes YxeA promotes the formation and expression of flagellum, and the obtained strain can enhance the immune recognition of host cells on Listeria monocytogenes, and can be used for preparing related preparations.

The invention belongs to the field of biological agriculture, and relates to application of flagellum-associated protein PbFAP-102 in plasmodiophora brassicae and a coding gene thereof in prevention and control of rape clubroot. According to the research, flagellar associated protein (PbFAP-102) which is expressed at a high level in each stage is screened from a plasmodiophora brassicae ZJ-1 transcriptome, an RNAi carrier of the gene is constructed, a corresponding transgenic plant is obtained, and a disease resistance test of plasmodiophora brassicae shows that the two PbFAP-102-RNAi transgenic lines both show better disease resistance phenotypes, and the two PbFAP-102-RNAi transgenic lines show better disease resistance phenotypes. Therefore, the flagellum-related protein PbFAP-102 of plasmodiophora brassicae can be used as a gene resource for resisting clubroot.

This invention relates to a polycyclic compound of formula (I) or a pharmaceutically acceptable salt thereof, wherein: Y, (A), L, (B), X, n, and m are as defined herein. These compounds are used to treat bacterial infections, particularly infections caused by flagellated bacteria. For example, they have practical applications in the fields of pharmaceuticals and agrochemicals.

The invention relates to the technical field of micro-nano, and discloses a drug-loaded microalgae motor as well as a preparation method and application thereof. The drug-loaded microalgae motor comprises a microalgae motor and a nano-particle drug loaded on the microalgae motor, the microalgae motor is connected with the nano-particle drug through electrostatic adsorption, and microalgae is natural or engineered unicellular algae with movement ability. The nanoparticle drug is formed by self-assembling natural polysaccharide and natural active substances after covalent cross-linking. According to the invention, the microalgae motor can autonomously move in the gastrointestinal tract by utilizing the flagellum movement of the microalgae, and is actively targeted to the colitis disease part, so that the local concentration of the medicine is improved, and the curative effect is enhanced. The microalgae motor combines the strong anti-oxidation / anti-inflammatory activity of astaxanthin and the biological activity of microalgae, and can synergistically relieve colitis in multiple ways.

The invention discloses ensifer Ensifer.sp and a method for producing L-carnitine through fermentation of the ensifer Ensifer.sp, and belongs to the technical field of biology. The strain is screened and separated from soil, is gram negative bacteria, is rod-shaped, has flagellum, can grow by taking betaine as a unique carbon and nitrogen source, and is preserved in the China Center for Type Culture Collection on September 25, 2025, the preservation number of the strain is CCTCC NO: M 20252114, under a shake flask condition, the concentration of L-carnitine is 6g / L, the molar conversion rate of a substrate is 99%, and under a fermentation tank loading condition, the concentration of the L-carnitine in the fermentation tank is 1L / L; the concentration of produced L-carnitine can reach 15.1 g / L, and the molar conversion rate of a substrate is 80.8%. An infrared spectrogram of a crystallization product extracted from fermentation liquor is consistent with that of an L-carnitine standard sample, and the specific rotation is-30.0 degrees. The invention provides the L-carnitine production method with small environmental pollution, and the L-carnitine production method has a good application prospect.

The application discloses a primer for homologous recombination and scarless deletion detection of Vibrio parahaemolyticus flagellum protein loci, wherein the Vibrio parahaemolyticus flagellum protein loci comprises flaA, flaB, flaC, flaD, flaE and flaF genes, and the primer is specifically shown in Table 1; a scarless deletion construction method of the Vibrio parahaemolyticus flagellum protein loci is also disclosed, and application of the scarless deletion Vibrio parahaemolyticus flagellum protein loci constructed by the method in regulating motility, biofilm formation, adhesion capacity, virulence and immunogenicity of Vibrio parahaemolyticus is disclosed. The primer provided by the application realizes efficient scarless genetic operation of the Vibrio parahaemolyticus flagellum protein loci; the scarless deletion flagellum protein loci provided by the application significantly affect the motility, biofilm formation, adhesion capacity, virulence and immunogenicity of Vibrio parahaemolyticus, and have application prospects in Vibrio parahaemolyticus disease prevention and control.