Oil-degradation oil-and-salt tolerance bacterial strain, and screening method and application thereof

A strain and oil technology, applied in microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of high cost, low oil condensation performance, high price, etc., and achieve shortened screening time, oil and salt resistance. Good, fast growth effect

Active Publication Date: 2013-07-17
航天圣诺(北京)环保科技有限公司
View PDF6 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical method is effective and can deal with a large area of ​​oil spill in a short time even in bad weather, but the disadvantage is that the use of dispersants and other chemical agents wastes energy and may cause secondary pollution; the oil condensation performance is low, The production process is complicated, the cost is high, and the price is expensive, so it is difficult to be applied in practice

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Oil-degradation oil-and-salt tolerance bacterial strain, and screening method and application thereof
  • Oil-degradation oil-and-salt tolerance bacterial strain, and screening method and application thereof
  • Oil-degradation oil-and-salt tolerance bacterial strain, and screening method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0036] Below in conjunction with the examples, the present invention is further described, the following examples are illustrative, not limiting, and the protection scope of the present invention cannot be limited by the following examples.

[0037] The technical route of the present invention is: make the collected soil sample into a solution with sterile physiological saline, vibrate on a shaker for 30 minutes to make a soil suspension, absorb and mix 10 mL of the soil suspension and add it to 100 mL of petroleum mass concentration 0.5% sterile mineral salt culture solution and cultured in a shaker (180 r / min, 30°C). After 3 days, 4 cycles of strain acclimatization (take 10 mL of the previous culture solution and add it to the newly prepared and sterilized mineral salt culture solution with different oil mass concentrations), the concentration of crude oil increased continuously during acclimation, from 0.5 - 2.5 g / 100 mL increase by 0.5 g each time. After the last acclimat...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to an oil-degradation oil-and-salt tolerance bacterial strain which is named as SY-1. The systematic name of the SY-1 is Escherichia coli, the file number is CGMCC (China General Microbiological Culture Collection Center No.7075, the file date is December 31, 2012, the file address is the Micro-organism Study of Chinese Academy of Sciences, No.3, No.1 Beichen west road, Chaoyang district, Beijing city, and the file unit is China General Microbiological Culture Collection Center. The oil-degradation oil-and-salt tolerance bacterial strain is good in oil and salt tolerance, is Gram-negative bacterium shaped in short rhabditiform and with flagellum and no spores and active, is in citrate and glucose ammonium salt positive property and can generate catalase without gelatinase. The bacterial strain is fast in growth, can grow in oil used as unique carbon source, and has the advantages of oil degradation.

Description

technical field [0001] The invention belongs to the field of microbial breeding and ecology, in particular to an oil-resistant and salt-tolerant bacterial strain for degrading petroleum, a screening method and application thereof. Background technique [0002] As the main energy source, petroleum has played a vital role in the development of human society, but it will inevitably pollute the environment during the process of oil extraction, transportation, loading and unloading, processing and use. According to incomplete statistics, there are about 1.0 ×10 10 kg of oil enters the soil or water through various channels, pollutes the groundwater, flows into the ocean, changes the aquatic environment, and threatens the aquatic animals, plants and marine life. With the continuous deterioration of the global environment, the safety of the ecological environment has attracted more and more attention. [0003] The harm of oil to the environment is mainly concentrated in the ocea...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02B09C1/10C12R1/19
Inventor 王昌禄张丽红王玉荣李贞景陈勉华
Owner 航天圣诺(北京)环保科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap