Oil-degradation oil-and-salt tolerance bacterial strain, and screening method and application thereof
An oil-resistant and salt-resistant technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of high cost, low oil-condensing performance, and high price, and achieve shortened screening time, oil-resistant and salt-resistant The effect of good sex and fast growth rate
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[0036] Below in conjunction with the examples, the present invention is further described, the following examples are illustrative, not limiting, and the protection scope of the present invention cannot be limited by the following examples.
[0037] The technical route of the present invention is: make the collected soil sample into a solution with sterile physiological saline, vibrate on a shaker for 30 minutes to make a soil suspension, absorb and mix 10 mL of the soil suspension and add it to 100 mL of petroleum mass concentration 0.5% sterile mineral salt culture solution and cultured in a shaker (180 r / min, 30°C). After 3 days, 4 cycles of strain acclimatization (take 10 mL of the previous culture solution and add it to the newly prepared and sterilized mineral salt culture solution with different oil mass concentrations), the concentration of crude oil increased continuously during acclimation, from 0.5 - 2.5 g / 100 mL increase by 0.5 g each time. After the last acclimat...
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