Hylotelephium erythrostictum rhizosphere cadmium-resistant Cupriavidus sp., and screening method and applications thereof

A technology of cadmium-resistant and copper-loving bacteria, which is applied to the cadmium-resistant strain of Sedum rhizosphere, copper-loving bacteria, can solve problems such as harming human health.

Inactive Publication Date: 2017-01-18
CHINA THREE GORGES CORPORATION +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Studies have found that if the cadmium content in the source water reaches 0.57-3.88mg/L, the down...

Method used

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  • Hylotelephium erythrostictum rhizosphere cadmium-resistant Cupriavidus sp., and screening method and applications thereof
  • Hylotelephium erythrostictum rhizosphere cadmium-resistant Cupriavidus sp., and screening method and applications thereof
  • Hylotelephium erythrostictum rhizosphere cadmium-resistant Cupriavidus sp., and screening method and applications thereof

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Experimental program
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Embodiment 1

[0025] Example 1 Isolation of sedum rhizosphere cadmium-resistant strain Cupriavidus sp.BCd1

[0026] Materials and Methods

[0027] 1. Medium

[0028] Bacterial culture medium: beef extract 3g, peptone 10g, NaCl 5g, distilled water 1000mL, pH7.0.

[0029] Prepare screening medium: the basic medium is 1000ml of bacterial culture medium, add 10ml of trace element solution and vitamin solution, sterilize at 121°C for 20min, cool to about 70°C, add cadmium solution mother liquor, so that the cadmium content in the medium is 10mg· L -1 . Incubate at 37°C.

[0030] The trace element solution is: MnSO 4 0.01g, ZnSO 4 0.05g, H 3 BO 3 0.01g, CaCl 2 0.01g, dilute to 1L, and store at 4°C in the dark.

[0031] The vitamin solution is: creatine 0.025g, ascorbic acid 0.025g, riboflavin 0.025g, citric acid 0.02g, dilute to 1L, and store in the dark at 4°C.

[0032] Cadmium solution mother liquor: CdSO 4 Formulated as Cd 2+ The concentration is 200mg·ml -1 The mother liquor...

Embodiment 2

[0035] The bacterial species identification of embodiment 2 bacterial strain BCd1

[0036] 1. Genomic DNA extraction

[0037] The above-mentioned strain BCd1 was mass-cultured according to conventional technical means, and then its genomic DNA was obtained.

[0038] 2. Identification method of 16S rDNA of strain BCd1

[0039] 2.1 PCR amplification, sequencing and phylogenetic tree construction of 16SrDNA of strain BCd1

[0040] 2.1. PCR amplification of 116S rDNA gene sequence

[0041] The primers at both ends of the amplified 16S rDNA gene sequence are universal primers: forward primer BSF8 / 20: 5'-AGAGTTTGATCCTGGCTCAG-3' (SEQ ID NO: 1) and reverse primer BSR1541 / 20: 5'-AAGGAGGTGATCCAGCCGCA-3' (SEQ ID NO: 2). The PCR reaction system was 50 μl, and the reaction conditions were denaturation at 94°C for 5 minutes; followed by 35 cycle reactions: denaturation at 94°C for 45 s, annealing at 50°C for 45 s, and extension at 72°C for 90 s; then extension at 72°C for 10 min, and fi...

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Abstract

The invention relates to hylotelephium erythrostictum rhizosphere cadmium-resistant Cupriavidus sp. BCd1, and a screening method and applications thereof, and more specifically relates to the cadmium-resistant Cupriavidus sp. BCd1 obtained via separation from hylotelephium erythrostictum rhizosphere. The hylotelephium erythrostictum rhizosphere cadmium-resistant Cupriavidus sp. BCd1 is stored in China General Microbiological Culture Collection Center on 1th December 2014, and preservation number is CGMCC No.10080. The 16S rDNA gene sequence is represented by graph 1. The hylotelephium erythrostictum rhizosphere cadmium-resistant Cupriavidus sp. BCd1 is Gram negative, belongs to aerobic coccobacillus, possesses flagellum and capsule, possesses relatively high resistance on heavy metal cadmium, and possesses practical significance and high engineering value in plant-microorganism combined remediation of cadmium heavy metal polluted soil.

Description

technical field [0001] The invention relates to sedum rhizosphere cadmium-resistant bacterial strain Cupriavidus sp. BCd1 and application thereof. Background technique [0002] The heavy metal cadmium is one of the important metal elements that cause environmental pollution. It will not only cause harm to plants, but also cause a certain degree of harm to the human body through the transmission and enrichment of the food chain. Japan's "itai disease" is caused by cadmium caused by poisoning. Cadmium mainly comes from industries such as electroplating, alloys, plastics, printing, and ceramics. Therefore, research on the treatment of cadmium-containing pollution is of great significance in the field of environmental protection. [0003] Human activities can cause cadmium to enter the soil in various ways, and the biggest characteristic of cadmium migration and transformation is that it cannot or is not easy to be decomposed and transformed by organisms and then excreted. Con...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/01
Inventor 林海龙范阳冯晶石田宇哲李德斌
Owner CHINA THREE GORGES CORPORATION
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