Sedum rhizosphere cadmium-resistant bacillus sp. strain, screening method and application thereof

A technology of Bacillus and cadmium resistance, which is applied to the cadmium-resistant strain of Bacillus in the rhizosphere of Sedum sedum, can solve problems such as endangering human health

Inactive Publication Date: 2015-11-04
CHINA THREE GORGES CORPORATION +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Studies have found that if the cadmium content in the source water reaches 0.57-3.88mg/L, the down...

Method used

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  • Sedum rhizosphere cadmium-resistant bacillus sp. strain, screening method and application thereof
  • Sedum rhizosphere cadmium-resistant bacillus sp. strain, screening method and application thereof
  • Sedum rhizosphere cadmium-resistant bacillus sp. strain, screening method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Isolation of cadmium-resistant bacterial strain Bacillus sp.BCd5

[0029] Materials and Methods

[0030] 1. Medium

[0031] Bacterial culture medium: beef extract 3g, peptone 10g, NaCl 5g, distilled water 1000mL, pH7.0.

[0032] Prepare the screening medium: the basic medium is 1000ml of bacterial culture medium, add 10mL of trace element solution and vitamin solution, sterilize at 121°C for 20min, cool to about 70°C, add cadmium solution mother liquor, so that the cadmium content in the medium is 10mg· L -1 . Incubate at 37°C.

[0033] The trace element solution is: MnSO 4 0.01g, ZnSO 4 0.05g, H 3 BO 3 0.01g, CaCl 2 0.01g, dilute to 1L, and store at 4°C in the dark.

[0034] The vitamin solution is: creatine 0.025g, ascorbic acid 0.025g, riboflavin 0.025g, citric acid 0.02g, dilute to 1L, and store in the dark at 4°C.

[0035] Cadmium solution mother liquor: CdSO 4 Formulated as Cd 2+ The concentration is 200mg·ml -1 The mother liquor was s...

Embodiment 2

[0038] The strain identification of embodiment 2 bacterial strain BCd5

[0039] 1. Genomic DNA extraction

[0040] The above-mentioned strain BCd5 was mass-cultured according to conventional technical means, and then its genomic DNA was obtained.

[0041] 2. Identification method of 16S rDNA of strain BCd5

[0042] 2.1 PCR amplification, sequencing and phylogenetic tree construction of 16S rDNA of strain BCd5

[0043] 2.1.1 PCR amplification of 16S rDNA gene sequence

[0044] The primers at both ends of the amplified 16S rDNA gene sequence are universal primers: forward primer BSF8 / 20: 5'-AGAGTTTGATCCTGGCTCAG-3' (SEQ ID NO: 1) and reverse primer BSR1541 / 20: 5'-AAGGAGGTGATCCAGCCGCA-3' (SEQ ID NO: 2). The PCR reaction system was 50 μl, and the reaction conditions were denaturation at 94°C for 5 minutes; followed by 35 cycle reactions: denaturation at 94°C for 45 s, annealing at 50°C for 45 s, and extension at 72°C for 90 s; then extension at 72°C for 10 min, and finally stor...

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Abstract

The invention relates to a sedum rhizosphere cadmium-resistant bacillus sp. BCd5 strain and its screening method. The invention specifically relates to a sedum rhizosphere cadmium-resistant bacillus sp. BCd5 strain which has been preserved in the China General Microbiological Culture Collection Center (CGMCC) on December 1st, 2014 with the preservation number being CGMCC NO. 10077. 16S rDMA gene sequence of the strain is as shown in the Figure 1. The strain is a Gram-negative bacterium, aerobasilus having flagellum and capsule. The strain has high resistance to cadmium and has practical significance and important engineering application value in plant-microbial remediation of cadmium-contaminated soil.

Description

technical field [0001] The invention relates to a cadmium-resistant bacillus (Bacillus sp.) BCd5 isolated from the rhizosphere of Sedum sedum and its application in plant-microorganism combined restoration of cadmium heavy metal-contaminated soil. Background technique [0002] The heavy metal cadmium is one of the important metal elements that cause environmental pollution. It will not only cause harm to plants, but also cause a certain degree of harm to the human body through the transmission and enrichment of the food chain. Japan's "itai disease" is caused by cadmium caused by poisoning. Cadmium mainly comes from industries such as electroplating, alloys, plastics, printing, and ceramics. Therefore, research on the treatment of cadmium-containing pollution is of great significance in the field of environmental protection. [0003] Human activities can cause cadmium to enter the soil in various ways, and the biggest characteristic of cadmium migration and transformation i...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/00B09C1/10C12R1/07
Inventor 林海龙范阳冯晶石田宇哲李德斌
Owner CHINA THREE GORGES CORPORATION
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