Detection kit for helicobacter pylori virulence protein antibody and detection method by using the same

A technology for detection of Helicobacter pylori and antibodies, applied in biological testing, measuring devices, material inspection products, etc., can solve problems such as not fully meeting the detection requirements, and achieve high specificity

Inactive Publication Date: 2012-10-10
TIANJIN TIANFOLUO BIOLOGICAL TECH
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Problems solved by technology

However, the Helicobacter pylori antigen involved in the test is a partially purified whole antigen of Hel

Method used

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  • Detection kit for helicobacter pylori virulence protein antibody and detection method by using the same
  • Detection kit for helicobacter pylori virulence protein antibody and detection method by using the same
  • Detection kit for helicobacter pylori virulence protein antibody and detection method by using the same

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Embodiment Construction

[0019] The present invention will be further described in detail below through the specific examples, the following examples are only descriptive, not restrictive, and cannot limit the protection scope of the present invention with this.

[0020] The invention provides a detection kit and a detection method for preparing an important virulence protein antibody detection kit of Helicobacter pylori. Kit includes microwell plate, cytotoxin-associated protein CagA, vacuolar toxin protein VacA, flagellin subunit A, flagellin subunit B, urease subunit A, urease subunit B, mouse or rabbit anti-human horseradish Peroxidase HRP-conjugated antibody (enzyme-labeled secondary antibody), negative serum control, positive serum control, substrate, stop solution, wash solution and diluent. The microwell plate is coated with the above-mentioned 6 kinds of virulence proteins of Helicobacter pylori, which can be coated separately or mixed.

[0021] During detection, the serum or plasma sample r...

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Abstract

The invention relates to a detection kit for helicobacter pylori virulence protein antibody and a detection method by using the same. The kit comprises a microwell plate, an enzyme-labeled secondary antibody, a negative control serum, a positive control serum, a substrate, a stop buffer and a washing liquor, wherein the microwell plate is coated with purified recombinant helicobacter pylori virulence proteins, the substrate is a solution containing 1% m/m 3, 3', 5, 5'-tetramethyl benzidine and 30% v/v hydrogen peroxide, and the recombinant helicobacter pylori virulence proteins are cytotoxin-associated protein CagA, vacuolating toxin protein VacA, flagellum protein subunit A, flagellum protein subunit B, urease subunit A, and urease subunit B. According to the invention, six purified helicobacter pylori virulence proteins are used as antigens which itself have high conservatism and specificity and are related to the symptom of a patient, so the kit has characteristics of high specificity, identification capacity for helicobacter pylori clinical strain virulence strength, and semi-quantitative detection capacity.

Description

technical field [0001] The invention relates to the fields of enzyme-linked immunosorbent adsorption and clinical diagnosis of Helicobacter pylori, in particular to a detection kit for the virulence protein antibody of Helicobacter pylori and a detection method thereof. Background technique [0002] Helicobacter pylori (Hp), discovered by Australian doctors in 1983, is a spiral-shaped, Gram-negative bacterium that colonizes the human stomach. Helicobacter pylori infection is an important cause of human gastrointestinal diseases, which can lead to chronic gastritis, severe atrophic gastritis, peptic ulcer and gastric cancer. 30-50% of the population in western countries has been infected with Helicobacter pylori, and the infection rate of Helicobacter pylori in my country is as high as 70-90%. With the increase of age, the infection rate tends to increase. 10% of infected patients will develop clinical symptoms, including gastritis, gastroduodenal ulcer, etc. If they do not ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/545
Inventor 金守光杨洪江
Owner TIANJIN TIANFOLUO BIOLOGICAL TECH
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