Efficient deodorizing bacteria and application thereof

A deodorizing bacteria, high-efficiency technology, applied in the direction of bacteria, special treatment targets, biochemical equipment and methods, etc., can solve the problems of high modification cost, inability to large-scale application, low microbial activity and adaptability, and achieve strong deodorization. The effect of odor efficacy

Active Publication Date: 2020-09-18
YANGZHOU HAICHENG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still defects such as low microbial activity and adaptability, and high cost of preparation and modification of microbial carrier fillers, so it cannot be applied on a large scale.

Method used

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  • Efficient deodorizing bacteria and application thereof
  • Efficient deodorizing bacteria and application thereof
  • Efficient deodorizing bacteria and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Screening and Physiological and Biochemical Identification of Deodorizing Bacteria

[0025] (1) Source of strain

[0026] The bacteria source used in the present invention comes from solid waste in landfills.

[0027] (2) Isolation of strains

[0028] Take 10g of solid waste from a landfill, put it into a triangular flask containing sterilized glass beads and 100mL of normal saline, shake it at 30°C and 160r / min for 2h, let it stand for 20min, take 1mL of the supernatant, and incinerate The bacterial physiological water was diluted to a concentration of 10 -1 The suspension was serially diluted 10 times, and 200 μL of the suspension with different dilutions was drawn, spread on the medium, and cultured at 30°C for 28-72 hours. The growth of the strain was observed, and a single colony was picked for culture. According to the shape and growth of the strains, the strains with different shapes were picked for streak culture and stored at low temperature.

[...

Embodiment 2

[0041] Embodiment 2: the species identification of bacterial strain HCSW-2

[0042]By amplifying the 16S rDNA of the strain, a 16S rDNA sequence with a length of 1527 bp was obtained, as shown in SEQ ID NO.1. As PCR primers, bacterial universal primers 7F 5'-CAGAGTTTGATCCTGGCT-3' and 1540R 5'-AGGAGGTGATCCAGCCGCA-3' were used. The amplification reaction was carried out with a PCR machine.

[0043] PCR reaction system (25 μL): 2.5 μL 10×PCR Buffer, 1 μL dNTPs (concentration: 2.5 mmol / L), 0.5 μL each of the two primers, 0.5 μL DNA template, 0.5 μL rTaq DNA polymerase (5 U / μL), add Double distilled water to 25 μL.

[0044] The PCR amplification program was: 94°C pre-denaturation for 4 min, 94°C denaturation for 45 s, 55°C renaturation for 45 s, 72°C extension for 1 min, a total of 30 cycles, and finally 72°C extension for 10 min, 1% agarose gel electrophoresis, and EB staining UV detection. The homology comparison was carried out by the program, and the result showed that the ...

Embodiment 3

[0045] Example 3: Preparation of Lactobacillus plantarum HCSW-2 dormant cells

[0046] Preparation of dormant cells of strain Lactobacillus plantarum HCSW-2: Centrifuge the strain Lactobacillus plantarum HCSW-2 cultured in the medium in Example 1 at 8000r / min, 4°C for 10min, wash 3 times with phosphate buffer, and use the same Resuspend the precipitate in the buffer solution, so that the concentration of dormant cells is 10 mg / L, and freeze it at 4 ° C to obtain the dormant cells of the bacterial strain Lactobacillus plantarum HCSW-2, and prepare them for use. The method of activation within 6 months is the method of Example 1 culture medium for 36 hours at 36°C.

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Abstract

The invention discloses efficient deodorizing bacteria and application thereof, and belongs to the technical fields of bioengineering and environmental engineering. The efficient deodorizing bacteriaare named HCSW-2, are identified to belong to Lactobacillus plantarum, and are preserved in the China General Microbiological Culture Collection Center, the preservation date is August 14th, 2019, andthe preservation number is CGMCC No.18384. The efficient deodorizing bacteria are Gram negative bacteria, and are facultative anaerobic, a growth temperature range is 10-42 DEG C, the thallus lengthis 1.5-5.0 [mu]m, the width is 0.5-1 [mu]m, the efficient deodorizing bacteria are in shapes of ball rods or lines, one ends of thalli have flagella, and no spores exist. The strain HCSW-2 has excellent deodorizing efficacy after being subjected to activation culture, and can be used for enhanced biological deodorization in landfill or compost treatment processes of solid waste of livestock manure, excess sludge, garbage and the like.

Description

technical field [0001] The invention belongs to the technical fields of biological engineering and environmental engineering, and more specifically relates to a high-efficiency deodorizing bacterium and its application. Background technique [0002] Malodorous gases include sulfur-containing compounds, nitrogen-containing compounds, halogens and their derivatives, hydrocarbons, and oxygen-containing organic compounds. They are a type of serious environmental pollutants. In addition to affecting people's perception and environmental experience, they also directly endanger human health. The stench can have a strong stimulating effect on the respiratory system, nervous system, circulatory system, and endocrine system. Odor pollution comes from a wide range of sources, involving industry, agricultural and animal husbandry production, and urban public facilities, such as livestock and poultry manure produced by livestock and poultry breeding, and surplus sludge produced by sewage...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F11/02C02F103/20C12R1/25
CPCC12N1/20C02F11/02C02F2103/20C02F2303/02C12R2001/25C12N1/205
Inventor 尹安伟龚劲松李昂宋丽明陈明
Owner YANGZHOU HAICHENG BIOTECH CO LTD
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