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Pyrethroid pesticide degrading bacteria and bactericide thereof

A technology for the degradation of pyrethroids and pesticides, which is applied in the field of biotechnology, can solve the problems of no degrading bacteria and bacteria agents, and achieve the effects of promoting the coordination and unification of pest control medicines and food safety, convenient use, and low production costs

Inactive Publication Date: 2011-02-16
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Fenvalerate is a pyrethroid pesticide that has been widely used in recent years, and no degrading bacteria specifically targeting fenvalerate pesticide residues have been found

Method used

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  • Pyrethroid pesticide degrading bacteria and bactericide thereof
  • Pyrethroid pesticide degrading bacteria and bactericide thereof
  • Pyrethroid pesticide degrading bacteria and bactericide thereof

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Embodiment Construction

[0020] Activate the pyrethroid pesticide-degrading bacteria ZS-S-01 strain of the present invention on a solid plate, measure its degradation performance, and inoculate it on the inclined surface of a test tube for later use. Inoculate the test tube seed of fenvalerate pesticide residue degrading bacteria ZS-S-01 in a 1000 mL shake flask containing 250 mL of Charpy's medium, shake at a constant temperature to the logarithmic phase, and prepare to inoculate the seed tank. The seed tank is 100L, the feeding volume is 80L, the medium formula is: 3% sucrose, NaNO 3 0.2%, KCl 0.05%, MgSO 4 0.05%, K 2 HPO 4 0.1%, Fe 2 (SO 4 ) 3 0.001%, peptone 0.05%, pH=7.0. 1.1Kg / cm after feeding 3 Under the pressure of 121 ℃, high-pressure damp heat sterilization, after cooling to 30 ℃, inoculate the above-mentioned cultured shake flask strains into 100L seed tanks according to the inoculation amount of 8% to 10%, and the ventilation rate of sterile air is 0.45~0.60m 3 / min, the stirri...

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Abstract

The invention discloses pyrethroid pesticide degrading bacteria and a bactericide thereof, belonging to the field of biological high-tech. The adopted strain ZS-S-01 is identified as Stenotrophomonas sp. with the preservation number of CCTCC M 2010095, wherein, the Genbank registry number of the 16S rDNA of the strain is HM016874. The main biological characteristics are as follows: the strain is Gram-negative and has multiple polar flagellum and cells in a short rod shape; and the bacterial colony is milky white and slight yellowish, thicker and non-transparent, the diameter is 0.5mm-1mm, the edge is regular and orderly, the middle part is slightly protruding, and the surface is smooth and bright without wrinkles. The bactericide of the invention has the advantages of low production cost, convenient use and good degradation effect, thus being applicable to the national fruit and vegetable production and export bases or places with green food brand marks at a large area. The bactericide is of great significance to promoting production of nuisance-free vegetables and green food.

Description

technical field [0001] The invention provides a pyrethroid pesticide degrading bacterium and its agent, belonging to the high-tech field of biology, degrading chemical pesticide residues by means of microorganisms, and suitable for the production of green and pollution-free agricultural products in modern agricultural production. Background technique [0002] Pyrethroid pesticides are a class of biomimetic insecticides that are artificially synthesized to simulate natural pyrethrins. They target nerve sodium ion channels and have the advantages of low dosage, high efficacy, broad insecticidal spectrum and low toxicity. It is widely used in the control of cotton, tea, rice, fruit trees, vegetables and other pests and household hygiene pests. Especially in recent years, due to the frequent occurrence of poisoning incidents of highly toxic pesticides such as organophosphorus and "green barriers" incidents due to excessive pesticide residues, countries all over the world have pa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A62D101/04A62D101/28C12N1/20C12R1/01A62D3/02
Inventor 钟国华胡美英陈少华刘晶晶黄文文
Owner SOUTH CHINA AGRI UNIV
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