Novel bacterial strain for highly effective degradation of chrysanthemum ester and organophosphorus pesticide and uses thereof

An organophosphorus, new strain technology, applied in applications, bacteria, microbe-based methods, etc., can solve problems such as large losses

Inactive Publication Date: 2008-03-12
许雷
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among the agricultural and sideline products exported by my country, the trade loss caused by pesticide residues exceeds tens of billions of dollars every year, which is a huge loss

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: JCX22D bacterial strain biodegradation permethrin pesticide

[0035] JCX22D was cultured in LB medium until it reached the logarithmic growth phase, and inserted into the basal salt medium with 200 mg / l of beta-cypermethrin, cyhalothrin, deltamethrin and bifenthrin at 2% inoculum, 28 ℃, 180rpm constant temperature oscillator shaking culture, sampling according to a certain culture time, the samples were immediately extracted with cyclohexane, gas chromatography (Trace GC) was used to determine the pesticide residues, and the detector was an electron capture device (ECD). The measurement results are shown in Table 4.

[0036] Table 4 Degradation rate of four pyrethroid pesticides by JCX22D strain

[0037] Types of pesticides

Embodiment 2

[0038] Embodiment 2: JCX22D bacterial strain biodegradation organophosphorus pesticide

[0039] JCX22D was cultured in LB medium to the logarithmic growth phase, and inoculated with phoxim, parathion-methyl, chlorpyrifos and diazinon at 200 mg / l basal salt medium at 28°C. , 180rpm constant temperature culture, sampling according to a certain culture time, cyclohexane extraction, using UV-VIS 8500 double-beam ultraviolet / visible spectrophotometer for wavelength scanning, and comparing the samples with the control to determine the degradation rate of pesticides. The measurement results are shown in Table 5.

[0040] Table 5 Degradation rate (%) of JCX22D bacterial strain to four kinds of organophosphorus pesticides

[0041] 24hr

Embodiment 3

[0042] Embodiment 3: JCX22D bacterial strain biodegrades abamectin

[0043] JCX22D was cultured in LB medium until it reached the logarithmic growth phase. Inoculate 2% of the inoculum into the basal salt medium with 200 mg / l abamectin, culture at 28°C and 180rpm at constant temperature, and take samples according to a certain culture time. Chloroform extraction, use UV-VIS 8500 double-beam ultraviolet / visible spectrophotometer for wavelength scanning, and determine the degradation rate of pesticides by comparing the samples with the control. The measurement results are shown in Table 5.

[0044] Table 5 JCX22D bacterial strain is to the degradation rate (%) of abamectin

[0045]

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Abstract

The invention pertains to the technical field of biologic degrading for chemical pesticide, and relates to a bacterial strain that can efficiently degrade Pyrethroid, Organophosphorus and vermectins pesticides and the application of the bacterial strain. The bacterial strain is an Acinetobacter sp. JCX22D bacterial strain, the biologic fature of the bacterial strain is Gram's negative, free from flagella, in form of short rod, is of esterase and lipase activity, free from oxidase activity, and can take use glucose and fructose. The bacterial strain is resistant to Ampicillin and low-concentration Spectinomycin and nalectin, and not resistant to rifamide. The degrading efficiency of the bacterial straine is more than 72% for high-efficiency cypermethrin, cyhalothrin, decamethrim, bifenthrin pesticid, is more than 70% for diazinon, parathion-methyl, phoxime and chlorpyrifos, and is 90% for vermectins. The bacterial strain can be used for biologically degrading above pesticides, and biologically recovering or cleaning any water body, soil and agricultural products that are polluted by pesticides.

Description

technical field [0001] The invention belongs to the technical field of biodegradation of chemical pesticides, and relates to a new bacterium capable of efficiently degrading pyrethrins, organophosphorus, and avermin and its use, and is used for beta-cypermethrin, cyhalothrin, deltamethrin, Biodegradation of bifenthrin, diazinon, methyl parathion, phoxim, chlorpyrifos, abamectin pesticides, and bioremediation and biopurification of water, soil and agricultural products polluted by these pesticides. Background technique [0002] Chemical insecticides are a group of toxic compounds used to control pests and play an important role in agricultural production. The output of pesticides and insecticides in my country accounts for 60% of the total output of pesticides in the country, and its main varieties are organophosphorus and pyrethroids. [0003] Pyrethroids and organophosphorus pesticides are widely used on vegetables, tea, and fruit trees in my country. While recovering yiel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N15/31A62D3/00C02F3/34B09C1/10C12R1/36
Inventor 许雷肖红莉张艳辉
Owner 许雷
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