Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Parathion-methyl degradation enzyme mutant as well as encoding gene and application thereof

A methyl parathion-degrading enzyme technology, applied in the field of methyl parathion-degrading enzyme mutants and genes encoding methyl parathion-degrading enzyme mutants, can solve protein separation and purification difficulties, cost increase, Can not be effectively secreted and other problems, to achieve the effect of a wide range of application prospects

Active Publication Date: 2017-05-17
北京森根比亚生物工程技术有限公司
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the gene is only expressed in the cell in the Pichia pastoris system and cannot be effectively secreted, which causes great difficulties and cost increases for subsequent protein separation and purification

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Parathion-methyl degradation enzyme mutant as well as encoding gene and application thereof
  • Parathion-methyl degradation enzyme mutant as well as encoding gene and application thereof
  • Parathion-methyl degradation enzyme mutant as well as encoding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 Preparation of methyl parathion degrading enzyme mutant

[0034] 1. Experimental materials

[0035] 1.1 Strains and plasmids

[0036] Plasmids pET30a(+)-ophc2, pET30a(+)-mph, strains E.coli BL21, E.coli Top10 and strain Pichia pastors GS115 were preserved by the laboratory, plasmid pPIC9 was purchased from Invitrogen, and vector pGM-T was purchased from Tiangen Biochemical Technology (Beijing) Co., Ltd.

[0037] 1.1.2 Reagents

[0038] Plasmid extraction and gel recovery kits were purchased from Axygen, and Taq polymeras were purchased from Tiangen Biochemical Technology (Beijing) Co., Ltd., T 4 - Ligase and restriction endonuclease were purchased from NEB Company, recombinase and Fast Pfu were purchased from Quanshijin Biotechnology Co., Ltd., tryptone (Tryptone) and yeast extract (Yeast extract) were purchased from Oxford Company, UK, and YNB was purchased from Beijing Jingke Hongda Biotechnology Co., Ltd., and other chemical reagents were of domestic...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a parathion-methyl degradation enzyme mutant as well as an encoding gene and application thereof and belongs to the field of construction and application of the parathion-methyl degradation enzyme mutant. The parathion-methyl degradation enzyme mutant with a secretion capacity, which is remarkably increased in yeast supernatant, is screened from a constructed yeast mutant library by adopting a DNA (Deoxyribonucleic Acid) shuffling technology. The invention further discloses a recombinant expression vector and a recombinant host cell, which contain a parathion-methyl degradation enzyme mutant encoding gene. According to the parathion-methyl degradation enzyme mutant disclosed by the invention, the secretion capacity of the parathion-methyl degradation enzyme mutant in the yeast supernatant is remarkably increased, and industrial production requirements can be met; and the parathion-methyl degradation enzyme mutant has an important application prospect in the aspect of degrading organic phosphorus.

Description

technical field [0001] The invention relates to a mutant of methyl parathion degrading enzyme, and further relates to a gene encoding the mutant of methyl parathion degrading enzyme, which belongs to the field of construction and application of methyl parathion degrading enzyme mutant. Background technique [0002] With the improvement of human life requirements and the increasing development of industrial production, many compounds that are artificially synthesized and difficult to be rapidly degraded by natural microorganisms have entered nature. Among them, organophosphorus pesticides produced in large quantities at home and abroad and widely used as insecticides can destroy the activity of acetylcholinesterase, thereby causing a series of neurotoxic symptoms and even death. Therefore, with the improvement of people's quality of life and the strengthening of environmental protection awareness, more and more attention has been paid to the environmental pollution and ecolog...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/16C12N15/55C12N15/81C12N1/19A62D3/02C12R1/84A62D101/04A62D101/28
CPCA62D3/02A62D2101/04A62D2101/28C12N9/16C12N15/81C12N2800/102C12Y301/08001
Inventor 王璋李光雷龚晓洁王洋洋袁道莉秦南南郭刚兴
Owner 北京森根比亚生物工程技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com