Rhodococcus ruber YMHL-1 capable of degrading nicosulfuron and applications thereof

A technology of YMHL-1 and nicosulfuron, which is applied in the bio-high field, can solve problems such as negative effects, and achieve the effects of low production and use costs, convenient use, and good removal effect

Active Publication Date: 2017-04-26
JIANGSU NANZI ENVIRONMENTAL PROTECTION SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although nicosulfuron has a very high control effect and good selectivity, however, part of it will remain in the natural environment such as soil, air and water d

Method used

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  • Rhodococcus ruber YMHL-1 capable of degrading nicosulfuron and applications thereof
  • Rhodococcus ruber YMHL-1 capable of degrading nicosulfuron and applications thereof
  • Rhodococcus ruber YMHL-1 capable of degrading nicosulfuron and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 The screening and isolation of nicosulfuron-degraded Rhodococcus erythrococcus YMHL-1

[0029] Get 3.0ml of activated sludge obtained from the waste water treatment tank of a pesticide factory production workshop producing nicosulfuron and add it into 100ml LB medium with a nicosulfuron concentration of 100mg / L. The formula is: every liter contains NaCl 10.00g / L, peptone 10.00g / L, yeast powder 5.00g / L, pH 7.0, shake culture at 220r / min, 37°C, transfer to fresh inorganic salts at 3% inoculum every 3 days In the culture medium, transfer 5 times in succession.

[0030] Take 1.0ml of the enriched bacteria solution obtained above, add 9.0ml of sterile water, and make 10 -1 The rich solution, and then draw 1.0ml prepared 10 -1 The enrichment solution was added to 9.0ml sterile water, mixed thoroughly to make 10 -2 The enrichment solution, and so on, carry out gradient dilution on the enrichment solution. By analogy, the enrichment solution was serially diluted. ...

Embodiment 2

[0032] Shake flask degradation experiment of embodiment 2 rhodococcus erythrococcus YMHL-1

[0033] 2.1 The effect of inoculum size on the degradation efficiency of YMHL-1

[0034] In the inorganic salt medium of 100mg / L nicosulfuron, its formula is: every liter contains 0.50g K 2 HPO 4 , 0.50gKH 2 PO 4 , 0.20g MgSO 4 ·7H 2 O, 1.00g NaCl, 1.00g (NH 4 ) 2 SO 4 , pH 7.0, YMHL-1 was inoculated in the culture medium according to the inoculum amount of 1%, 2%, 3%, 4%, 5%, and cultured with shaking at 37°C, and samples were taken for measurement after 72 hours. The result is given by figure 2 It can be seen that when 1% is inoculated, the degradation rate is 91%. With the increase of the inoculum amount, the degradation rate of nicosulfuron has a slight increase. Considering the reasons of bacterial growth and economy, the inoculum amount of 1% is adopted The requirement can be met.

[0035] 2.2 The effect of pH on the degradation efficiency of YMHL-1

[0036] The pH valu...

Embodiment 3

[0038] Embodiment 3 contains the preparation of rhodococcus rubella YMHL-1 bacterial agent

[0039] The original species of the nicosulfuron-methyl-degrading Rhodococcus erythrococcus YMHL-1 screened and isolated in Example 1 was activated on a petri dish, and the degradation performance was measured, and then inoculated on the inclined surface of the test tube for later use. The test tube seed was inoculated in a 1000ml shake flask containing 200ml of LB medium containing nicosulfuron. The formula of LB medium was: nicosulfuron 100mg / L, yeast powder 5.00g / L, peptone 10.00g / L, NaCl10. 00g / L, pH 7.0, constant temperature shaking culture to logarithmic phase, ready to inoculate seed tanks. 500 liters of seed tanks, 400 liters of feeding capacity, the medium formula is: nicosulfuron 0.1%, glucose 0.5%, (NH 4 ) 2 SO 4 1%, K 2 HPO 4 0.2%, MgSO 4 0.05%, NaCl 0.01%, CaCO 3 0.3%, yeast extract 0.02%, pH 7.2-7.5. After the feeding is completed, 121°C high-pressure damp heat...

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Abstract

The invention discloses rhodococcus ruber YMHL-1 capable of degrading nicosulfuron and applications thereof. The invention provides a strain namely rhodococcus ruber YMHL-1 capable of degrading residual nicosulfuron in wastewater, and the identification shows that the strain belongs to Rhodococcus. The strain is preserved in China General Microbiological Culture Collection Center, CGMCC in February 9th, 2015; and the preservation number is CGMCC No. 10542. The main biological characteristics are as follows: the Gram staining reaction is negative; the bacterial colony is in an orange yellow color, is in a round shape, and is opaque, the surface of the bacterial colony is rough and dry; the cell is in a sphere shape or short bar shape, does not have any flagellum, is motionless, and does not generate any spore; the strain is aerobic and chemoheterotrophic and cannot liquefy gelatin and hydrolyze starch; the enzyme contact reaction is positive, the V.P. reaction is positive, the methyl red reaction is negative; the strain cannot degrade casein, cannot reduce nitrates; and the strain can produce acids from glucose, can utilize glucose, fructose, and citrates, cannot utilize mannose, arabinose, and raffinose, and can reduce more than 90% of antibiotics in water through direct application.

Description

technical field [0001] The invention belongs to the field of biological high technology, uses microorganisms to degrade chemical pesticides, is suitable for the production and processing of green and pollution-free agricultural products in modern agricultural production, and specifically relates to a nicosulfuron-degrading rhodococcus erythrococcus YMHL-1 and its application. Background technique [0002] With the improvement of people's living standards and the enhancement of environmental protection awareness, the whole society is paying more and more attention to the direct harm and potential impact of pesticide residues. People urgently need clean and green agricultural products without pesticide residue pollution. However, judging from the current form of agricultural production and population status in our country, the pollution-free production model at the cost of stopping the use of pesticides and reducing agricultural output is not feasible. Pesticides will continue ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C02F3/34B09C1/10C12R1/01A62D101/04C02F101/38C02F101/34
CPCA62D3/02A62D2101/04B09C1/10C02F3/34C02F2101/306C02F2101/34C02F2101/38C02F2101/40C12N1/20C12N1/205C12R2001/01
Inventor 陈立伟胡江张永乐董晓梦
Owner JIANGSU NANZI ENVIRONMENTAL PROTECTION SCI & TECH
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