Shewanella and application thereof in microbiological fuel cell

A technology of Shewanella and fuel cells, applied in the biological field, can solve the problems of microbial fuel cells such as lack of power generation efficiency and long-term stability, research sluggishness, etc., to achieve wide salt ion concentration tolerance, high power generation activity, broad Effect of Salt Ion Concentration Tolerance Activity

Inactive Publication Date: 2010-11-10
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the lack of power generation efficiency and long-term stabi...

Method used

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  • Shewanella and application thereof in microbiological fuel cell
  • Shewanella and application thereof in microbiological fuel cell
  • Shewanella and application thereof in microbiological fuel cell

Examples

Experimental program
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Embodiment 1

[0064] Embodiment 1: Screening of EP1 bacterial strain

[0065] Marine sediment samples were collected from the bay near Xiamen, China. Take 2 grams of soil samples and suspend them in 250 milliliters of microbial fuel cell anode electrode solution for enrichment culture. The composition of anolyte medium (MM1) is: 1000 milliliters of deionized water, 1.30 g K 2 HPO 4 , 0.45g KH 2 PO 4 , 0.19g (NH 4 ) 2 SO 4 , 0.25g MgSO 4 , 10ml Wolfe's Minerals, 0.05g CaCl 2 , 0.02g L-arginine, 0.02g glutamic acid, 0.02g serine and 0.10g yeast powder, wherein 10mmol / L of sodium lactate (DL-lactate) was added as an electron donor. The composition of the catholyte of the fuel cell is the same as that of the anolyte except that no amino acid and yeast powder are added, and corresponding sodium chloride is added to the catholyte to balance the osmotic pressure with the anolyte. The sampling H-shaped structure of the microbial fuel cell device is composed of two screw cap glass bottles,...

Embodiment 2

[0066] Example 2: Acquisition and induction of EP1 electrogenic seeded cells

[0067] Pick EP1 strain colonies cultured on LB plates, inoculate them into 20 ml of MM1 medium, add 20mmol / L sodium lactate as electron donor and carbon source, cultivate at 30°C for 12 hours at 180 rpm, and press 0.1% Add it to 100 ml of anaerobic MM1 medium, add 20mmol / L sodium lactate and 20mmol / L sodium fumarate as electron donor and electron acceptor respectively, and culture it statically at 30°C for 12 hours, then Inoculate 0.1% into anaerobic MM1 medium with 50mmol / L ferric citrate and 20mmol / L sodium lactate as electron acceptor and donor, culture at 30°C for 12 hours, and centrifuge under anaerobic environment to obtain bacteria. Place the cells in 100 ml of anaerobic MM1 medium, mix and shake for 1 to 2 hours, and centrifuge to obtain the cells again.

Embodiment 3

[0068] Example 3: Obtaining EP1 Active Stem Cells

[0069] Pick EP1 cells preserved in glycerol, inoculate them into 5 ml of LB medium, culture on a shaker at 30°C at 180rpm for 12 hours, inoculate 0.1% inoculum into 20ml of MM1 medium containing 20mmol / L sodium lactate, and culture at 30°C at 180rpm for 12 hours hours, 0.1% was inoculated into anaerobic MM1 medium with sodium fumarate and sodium lactate as electron acceptors and donors, and after static culture at 30°C for 12 hours, 0.1% inoculum was inoculated into 5000ml of ferric citrate and sodium lactate as an electron acceptor and an electron donor in anaerobic MM1 medium, cultured statically at 30°C for 12-16 hours, and centrifuged under anaerobic environment to obtain bacterial cells. Place the cells in 100 ml of anaerobic MM1 medium, mix and shake, then centrifuge to collect the cells again. Freeze and vacuum dry to obtain active stem cells.

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Abstract

The invention discloses shewanella and application thereof in a microbiological fuel cell, and relates to the technical field of biology. The shewanella is Shewanella marisflavi EP1 which is preserved in 'China Center for Type Culture Collection' in January 2009 with the preservation number of CCTCC M 209016. The shewanella is characterized in that: the shewanella is gram-negative; the thalli are rod-shaped and straight; two ends are circular; the diameter is 0.3 to 1.0 micro; the length is 2.5 to 6.0 micros; the thalli can singly exist or are arranged in pair or in a short-chain shape; and the thalli have autogenetic flagella and can move. The shewanella is separated from offshore ocean sediments, has wide salt ion concentration tolerance activity, and can generate power in electrode liquid at the concentration of up to 8 percent. Therefore, the internal resistance of the microbiological fuel cell is effectively reduced.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a Shewanella bacterium and its application in microbial fuel cells. Background technique [0002] Energy is one of the main material foundations supporting the development of modern civilized society. At the same time, energy is also an important topic worldwide. In the second half of the 20th century, due to rapid population growth and rapid economic development, human society consumed limited resources at an unprecedented rate. resource. In 50 years, the world population has increased from 2.5 billion to over 6 billion, an increase of nearly 1.5 times. During the same period, the annual consumption of oil has increased by 6.3 times, the consumption of coal has increased by 2.6 times, and the consumption of natural gas has increased by 13.5 times. With the increasing depletion of these traditional fossil energy sources, energy issues have increasingly become the focus o...

Claims

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Application Information

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IPC IPC(8): C12N1/20H01M8/16H01M4/90C12R1/01
CPCY02E60/527Y02E60/50
Inventor 章晓波黄杰勋吴穗洁
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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