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Bacterium of degrading residual of organophorus pespared agent of bacterium

A pesticide residue degradation and bacterial strain technology, which is applied in the field of high biotechnology, can solve the problems of narrow degradation spectrum and limited use of bacterial strains, and achieve the effects of low production and use costs, reduced production and use costs, and protection of the ecological environment

Inactive Publication Date: 2005-01-12
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

In the prior art, our research group already has an invention patent for degrading bacteria (ZL96106317.3, publication number: CN1145408A, publication date: March 19, 1997), which is a strain of Bacillus J5, which can effectively degrade methylamine Phosphorus, but the strain has a narrow degradation spectrum and can only degrade one pesticide, methamidophos, so its use is limited

Method used

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  • Bacterium of degrading residual of organophorus pespared agent of bacterium
  • Bacterium of degrading residual of organophorus pespared agent of bacterium

Examples

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Embodiment Construction

[0014] Examples of the present invention are described below.

[0015] The original seed of the microbial organophosphorus pesticide residue-degrading bacteria DLL-1 of the present invention is activated on a petri dish, and its degradation performance is measured, and it is inoculated on the inclined surface of a test tube for later use. The test tube seeds were inoculated into 1000ml shake flasks containing 200ml broth medium, cultured with constant temperature shaking until logarithmic phase, and prepared to inoculate seed tanks. 500 liters of seed tanks, 400 liters of feeding capacity, the medium formula is: glucose 0.8%, (NH 4 ) 2 SO 4 1%, K 2 HPO 4 0.2%, MgSO 4 0.05%, NaCl 0.01%, CaCO 3 0.3%, yeast extract 0.02%, pH 7.2-7.5. After the feeding is completed, 121°C high-pressure damp heat sterilization, after cooling to 33°C, inoculate the above-mentioned cultivated shake flask strains into a 500-liter seed tank according to the inoculum amount of 10%, and cultiv...

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Abstract

A bacterial strain for eliminating organophosphorous residue is Granis staining reaction negative bacteria DLL-1 identified as pseudomonas putida. Its biological feature is G- with thallus being short bar shape, tail end being oval and single end being grown thickly with flagellum. The Genbank landing number of 16S rDNA for the bacterial strian is AF 447394.

Description

1. Technical field [0001] The invention relates to an organophosphorus pesticide residue degrading bacterium and a bacterial agent produced therefrom, which belong to the high-tech field of biology, use microorganisms to degrade chemical pesticide residues, and are suitable for the production and processing of green and pollution-free agricultural products in modern agricultural production. 2. Technical background [0002] The use of chemical pesticides has greatly promoted the development of modern agriculture, restored a large number of losses caused by diseases and insect pests for agricultural production, and greatly improved the labor efficiency and mechanization degree of agricultural production, playing a role in the modernization of agricultural production. immeasurable effect. But at the same time, pesticides are some toxic and harmful chemicals. After they enter the ecological environment, they not only kill the organisms that are harmful to humans, but also cause ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
Inventor 李顺鹏刘智沈标洪青何健
Owner NANJING AGRICULTURAL UNIVERSITY
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