Bacillus licheniformis capable of producing beta-mannanase
A technology of Bacillus licheniformis and mannanase, which is applied in the directions of microorganism-based methods, enzymes, bacteria, etc., can solve the problems of low β-mannanase enzyme activity and the like
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specific Embodiment approach 1
[0010] Specific Embodiment 1: In this embodiment, Bacillus licheniformis HDGLJT-01, which can produce β-mannanase, has been identified as a new species of Bacillus licheniformis, and it is preserved in the China Center for Type Culture Collection, with a preservation address It is from Wuhan University in Wuhan City, with a preservation date of November 29, 2009 and a preservation number of CGTCC No.M209288; the Bacillus licheniformis that can produce β-mannanase is a Gram-positive facultative anaerobic bacterium with a width of 0.5 ~0.8μm, length 1.5~2.0μm, no flagella, mesophytic ellipsoid spores, 10% salt tolerance, growth temperature range 30℃~55℃, cells arranged singly or in chains; can produce β-mannan The colony of Bacillus licheniformis growing on the solid medium is milky white, round, with irregular edges, rough and opaque surface, and the diameter of the colony is 0.5-1.5mm.
[0011] The length of the 16S rDNA sequence of Bacillus licheniformis that can produce β-ma...
specific Embodiment approach 2
[0014] Specific embodiment 2: In this embodiment, Bacillus licheniformis HDGLJT-01 capable of producing β-mannanase is screened from the retting solution of the main biological stage. Screening was carried out according to the following steps: take 2 mL of the retting liquid in the main biological stage and place it in 100 mL of enrichment medium for culture, enrich and cultivate for 2 days in an environment of 37 ° C and 160 r / min; then inoculate on the konjac flour separation medium, After culturing at 37°C for 2 days, a single colony was obtained, which was Bacillus licheniformis.
[0015] In the present embodiment, the enrichment medium consists of 5g konjac flour, 1g K 2 HPO 4 ·3H 2 O and 1000mL distilled water, adjust the pH value to 7.2, and sterilize at 121°C for 15min.
[0016] In the present embodiment, the konjac flour separation medium is made of 5g konjac flour, 10g peptone, 2g yeast extract, 0.2g MgCl 2 ·6H 2 O, 1g K 2 HPO 4 ·3H 2 O. 20g agar powder and 1...
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