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Bacillus licheniformis capable of producing beta-mannanase

A technology of Bacillus licheniformis and mannanase, which is applied in the directions of microorganism-based methods, enzymes, bacteria, etc., can solve the problems of low β-mannanase enzyme activity and the like

Inactive Publication Date: 2011-05-25
HEILONGJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the problem of low enzyme activity in the production of β-mannanase by fungi, and provide a Bacillus licheniformis that can produce β-mannanase

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0010] Specific Embodiment 1: In this embodiment, Bacillus licheniformis HDGLJT-01, which can produce β-mannanase, has been identified as a new species of Bacillus licheniformis, and it is preserved in the China Center for Type Culture Collection, with a preservation address It is from Wuhan University in Wuhan City, with a preservation date of November 29, 2009 and a preservation number of CGTCC No.M209288; the Bacillus licheniformis that can produce β-mannanase is a Gram-positive facultative anaerobic bacterium with a width of 0.5 ~0.8μm, length 1.5~2.0μm, no flagella, mesophytic ellipsoid spores, 10% salt tolerance, growth temperature range 30℃~55℃, cells arranged singly or in chains; can produce β-mannan The colony of Bacillus licheniformis growing on the solid medium is milky white, round, with irregular edges, rough and opaque surface, and the diameter of the colony is 0.5-1.5mm.

[0011] The length of the 16S rDNA sequence of Bacillus licheniformis that can produce β-ma...

specific Embodiment approach 2

[0014] Specific embodiment 2: In this embodiment, Bacillus licheniformis HDGLJT-01 capable of producing β-mannanase is screened from the retting solution of the main biological stage. Screening was carried out according to the following steps: take 2 mL of the retting liquid in the main biological stage and place it in 100 mL of enrichment medium for culture, enrich and cultivate for 2 days in an environment of 37 ° C and 160 r / min; then inoculate on the konjac flour separation medium, After culturing at 37°C for 2 days, a single colony was obtained, which was Bacillus licheniformis.

[0015] In the present embodiment, the enrichment medium consists of 5g konjac flour, 1g K 2 HPO 4 ·3H 2 O and 1000mL distilled water, adjust the pH value to 7.2, and sterilize at 121°C for 15min.

[0016] In the present embodiment, the konjac flour separation medium is made of 5g konjac flour, 10g peptone, 2g yeast extract, 0.2g MgCl 2 ·6H 2 O, 1g K 2 HPO 4 ·3H 2 O. 20g agar powder and 1...

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Abstract

The invention discloses bacillus licheniformis capable of producing beta-mannanase, which relates to bacillus licheniformis. The invention solves the low enzymatic activity problem of the conventional production of beta-mannanase by fungi. The bacillus licheniformis capable of producing beta-mannanase belongs to a new bacillus licheniformis variety, and was collected in China Center for Type Culture Collection at Wuhan University in Wuhan on November 29th, 2009, with a collection number of CGTTC No.M209288; the bacillus licheniformis capable of producing beta-mannanase is Gram-positive and anaerobic bacillus, is 0.5 to 0.8-micrometer wide, 1.5 to 2.0-micrometer long and does not have flagella and mesic elliptic spores. The beta-mannanase produced by the bacillus licheniformis is acidic mannanase, as is proved by a test. The molecular weight of the beta-mannanase is about 38kD. The beta-mannanase has higher high temperature resistance activity, and the highest activity of the beta-mannanase is 5168U / mL.

Description

technical field [0001] The present invention relates to a bacillus licheniformis. Background technique [0002] β-mannanase is an endohydrolase that hydrolyzes the main chain of 1,4-β-D-mannopyranose. , galactoglucomannan and mannan. It is widely used in food, medicine, papermaking, textile printing and dyeing, oil exploration and biological research technology, and is a very important tool enzyme. The strains used in the current industrial production are mostly fungi, and the enzyme activity is only 2300U / mL. Contents of the invention [0003] The purpose of the present invention is to solve the problem of low enzyme activity in producing β-mannanase by fungi at present, and provide a bacillus licheniformis capable of producing β-mannanase. [0004] The Bacillus licheniformis (Bacillus licheniformis) HDGLJT-01 that can produce β-mannanase in the present invention is identified as a new species of Bacillus licheniformis (Bacillus), and it is preserved in the China Type ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/42C12R1/10
Inventor 葛菁萍平文祥宋刚凌宏志赵丹高冬妮
Owner HEILONGJIANG UNIV
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