Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of preparation method of neutral protease

A technology of neutral protease and fermentation medium, applied in the direction of microorganism-based methods, biochemical equipment and methods, hydrolytic enzymes, etc., can solve the problems of low enzyme activity, improve enzyme activity, promote fermentation enzyme production, and solve enzyme The effect of low vitality

Active Publication Date: 2021-12-21
内蒙古溢多利生物科技有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention proposes a preparation method of neutral protease, which solves the problem of low enzyme activity of neutral protease produced by microbial fermentation in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of preparation method of neutral protease
  • A kind of preparation method of neutral protease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A preparation method for neutral protease, comprising the following steps:

[0033] A, Bacillus subtilis seed liquid is inoculated into fermentation medium according to 5% inoculation amount and carries out fermentation culture, and fermentation medium comprises the component of following parts by weight: 40 parts of sweet potato starches, 25 parts of bean cake powders, 7 powders of pumpkin powders, 1.3 parts of magnesium humate, 1 part of calcium complexate, 0.7 parts of dipotassium hydrogen phosphate, 1.2 parts of ammonium chloride, 1.1 parts of sodium nitrate, 450 parts of water, the pH value of the fermentation medium is 6.6; the culture temperature is 27°C , the culture time is 14h, obtains initial fermented liquid;

[0034] B. Add feed medium feed to the primary fermentation liquid, feed medium includes the following components by weight: 1 part of methanol, 10 parts of carrot powder, 8 parts of shrimp shell powder, 14 parts of glucose, 1.2 parts of diglyceride p...

Embodiment 2

[0040] A preparation method for neutral protease, comprising the following steps:

[0041] A, Bacillus subtilis seed liquid is inoculated into fermentation medium according to 6% inoculation amount and carries out fermentation culture, and fermentation medium comprises the component of following weight portion: 40 parts of sweet potato starches, 25 parts of bean cake powders, 7 powders of pumpkin powders, 1.3 parts of magnesium humate, 1 part of calcium complexate, 0.7 parts of dipotassium hydrogen phosphate, 1.2 parts of ammonium chloride, 1.1 parts of sodium nitrate, 450 parts of water, the pH value of the fermentation medium is 6.6; the culture temperature is 27°C , the culture time is 15h, obtains initial fermented liquor;

[0042] B. Add feed medium feed to the primary fermentation liquid, feed medium includes the following components by weight: 1 part of methanol, 10 parts of carrot powder, 8 parts of shrimp shell powder, 14 parts of glucose, 1.2 parts of diglyceride pa...

Embodiment 3

[0048] A preparation method for neutral protease, comprising the following steps:

[0049] A, Bacillus subtilis seed liquid is inoculated into fermentation medium according to 8% inoculation amount and carries out fermentation culture, and fermentation medium comprises the component of following parts by weight: 40 parts of sweet potato starches, 25 parts of bean cake powders, 7 powders of pumpkin powders, 1.3 parts of magnesium humate, 1 part of calcium complexate, 0.7 parts of dipotassium hydrogen phosphate, 1.2 parts of ammonium chloride, 1.1 parts of sodium nitrate, 450 parts of water, the pH value of the fermentation medium is 6.6; the culture temperature is 27°C , the culture time is 16h, obtains initial fermented liquid;

[0050] B. Add feed medium feed to the primary fermentation liquid, feed medium includes the following components by weight: 1 part of methanol, 10 parts of carrot powder, 8 parts of shrimp shell powder, 14 parts of glucose, 1.2 parts of diglyceride p...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of fermentation, and proposes a preparation method of neutral protease, comprising the following steps: A, inoculating the Bacillus subtilis seed liquid into a fermentation medium for fermentation and cultivation, the cultivation temperature is 25-28°C, and the cultivation time is 25°C. 14 to 16 hours to obtain the primary fermentation liquid; B, adding feed medium to the primary fermentation liquid and culturing for 12 to 15 hours to obtain the fermentation liquid; C, the fermentation liquid was flocculated, finely filtered, concentrated by ultrafiltration, and stabilized to obtain the medium Protease finished product; the pH value of the fermentation medium is 6.5-6.8, which includes the following components by weight: 30-50 parts of sweet potato starch, 20-30 parts of bean cake powder, 5-10 parts of pumpkin powder, and 1.2 parts of magnesium humate ~1.5 parts, 0.8~1.2 parts of calcium complex proteinate, 0.5~1.2 parts of dipotassium hydrogen phosphate, 1~1.5 parts of ammonium chloride, 1~1.3 parts of sodium nitrate, 400~500 parts of water. Through the above technical scheme, the problem of low enzymatic activity of the neutral protease produced by the microbial fermentation method in the prior art is solved.

Description

technical field [0001] The invention belongs to the technical field of fermentation and relates to a preparation method of neutral protease. Background technique [0002] Neutral protease belongs to a kind of hydrolytic enzyme, which can catalyze the hydrolysis of macromolecular proteins into small molecular peptides or amino acids and other products, and promote the absorption and utilization of proteins. Due to its fast catalytic reaction speed, no industrial pollution, and wide adaptability to catalytic reaction conditions, etc. And advantages, are widely used in food, feed, cosmetics, nutritional health products, detergents and other industries. [0003] The production of neutral protease can be extracted from animal and plant tissues. Plants as the source of protease will be affected by factors such as climatic conditions and the size of available land area, while animals are used as the source of protease. The size of its output mainly depends on The number of animals...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/50C12N1/20C12R1/125
CPCC12N9/50C12N1/20
Inventor 程礼海朱报常颜学锋汪东武张少敏
Owner 内蒙古溢多利生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com