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Preparation method of neutral protease

A neutral protease and seed technology, applied in the field of fermentation, can solve problems such as low enzyme activity, and achieve the effects of improving enzyme activity, improving stability, and promoting fermentation magnesium production.

Active Publication Date: 2020-09-11
内蒙古溢多利生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention proposes a preparation method of neutral protease, which solves the problem of low enzyme activity of neutral protease produced by microbial fermentation in the prior art

Method used

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Embodiment 1

[0032] A preparation method for neutral protease, comprising the following steps:

[0033] A, Bacillus subtilis seed liquid is inoculated into fermentation medium according to 5% inoculation amount and carries out fermentation culture, and fermentation medium comprises the component of following parts by weight: 40 parts of sweet potato starches, 25 parts of bean cake powders, 7 powders of pumpkin powders, 1.3 parts of magnesium humate, 1 part of calcium complexate, 0.7 parts of dipotassium hydrogen phosphate, 1.2 parts of ammonium chloride, 1.1 parts of sodium nitrate, 450 parts of water, the pH value of the fermentation medium is 6.6; the culture temperature is 27°C , the culture time is 14h, obtains initial fermented liquor;

[0034] B. Add feed medium feed to the primary fermentation liquid, feed medium includes the following components by weight: 1 part of methanol, 10 parts of carrot powder, 8 parts of shrimp shell powder, 14 parts of glucose, 1.2 parts of diglyceride p...

Embodiment 2

[0040] A preparation method for neutral protease, comprising the following steps:

[0041] A, Bacillus subtilis seed liquid is inoculated into fermentation medium according to 6% inoculation amount and carries out fermentation culture, and fermentation medium comprises the component of following parts by weight: 40 parts of sweet potato starches, 25 parts of bean cake powders, 7 powders of pumpkin powders, 1.3 parts of magnesium humate, 1 part of calcium complexate, 0.7 parts of dipotassium hydrogen phosphate, 1.2 parts of ammonium chloride, 1.1 parts of sodium nitrate, 450 parts of water, the pH value of the fermentation medium is 6.6; the culture temperature is 27°C , the culture time is 15h, obtains the primary fermentation liquid;

[0042] B. Add feed medium feed to primary fermentation liquid, feed medium comprises the following components by weight: 1 part of methanol, 10 parts of carrot powder, 8 parts of shrimp shell powder, 14 parts of glucose, 1.2 parts of diglycerid...

Embodiment 3

[0048] A preparation method for neutral protease, comprising the following steps:

[0049] A, Bacillus subtilis seed liquid is inoculated into fermentation medium according to 8% inoculation amount and carries out fermentation culture, and fermentation medium comprises the component of following parts by weight: 40 parts of sweet potato starches, 25 parts of bean cake powders, 7 powders of pumpkin powders, 1.3 parts of magnesium humate, 1 part of calcium complexate, 0.7 parts of dipotassium hydrogen phosphate, 1.2 parts of ammonium chloride, 1.1 parts of sodium nitrate, 450 parts of water, the pH value of the fermentation medium is 6.6; the culture temperature is 27°C , the culture time is 16h, obtains initial fermented liquid;

[0050] B. Add feed medium feed to the primary fermentation liquid, feed medium includes the following components by weight: 1 part of methanol, 10 parts of carrot powder, 8 parts of shrimp shell powder, 14 parts of glucose, 1.2 parts of diglyceride p...

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Abstract

The invention belongs to the technical field of fermentation, and provides a preparation method of neutral protease. The preparation method of neutral protease comprises the steps of: A, inoculating bacillus subtilis seed solution into a fermentation culture medium to perform fermentation culture, wherein the culture temperature is 25-28 DEG C; the culture time is 14-16 h; and primary fermentationsolution is obtained; B, adding a supplementary culture medium into the primary fermentation solution to culture for 12-15 h, so that fermentation solution is obtained; and C, obtaining a neutral protease product after performing flocculation, refined filtration, ultra-filtration concentration and stabilizing treatment on the fermentation solution, wherein the pH value of the fermentation culturemedium is 6.5-6.8; and the fermentation culture medium comprises the following components in parts by weight: 30-50 parts of sweet potato starch, 20-30 parts of soybean cake powder, 5-10 parts of pumpkin powder, 1.2-1.5 parts of magnesium humate, 0.8-1.2 parts of calcium caseinate, 0.5-1.2 parts of dipotassium phosphate, 1-1.5 parts of ammonia chloride, 1-1.3 parts of sodium nitrate, and 400-500parts of water. By means of the technical scheme, the problem that the neutral protease produced by the microbial fermentation method has relatively low enzyme activity in the prior art is solved.

Description

technical field [0001] The invention belongs to the technical field of fermentation and relates to a preparation method of neutral protease. Background technique [0002] Neutral protease belongs to a kind of hydrolytic enzyme, which can catalyze the hydrolysis of macromolecular proteins into small molecular peptides or amino acids and other products, and promote the absorption and utilization of proteins. Due to its fast catalytic reaction speed, no industrial pollution, and wide adaptability to catalytic reaction conditions, etc. And advantages, are widely used in food, feed, cosmetics, nutritional health products, detergents and other industries. [0003] The production of neutral protease can be extracted from animal and plant tissues. Plants as the source of protease will be affected by factors such as climatic conditions and the size of available land area, while animals are used as the source of protease. The size of its output mainly depends on The number of animals...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/50C12N1/20C12R1/125
CPCC12N9/50C12N1/20
Inventor 程礼海朱报常颜学锋汪东武张少敏
Owner 内蒙古溢多利生物科技有限公司
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