High-efficiency denitrification and dephosphorization bacterial strain C18
A high-efficiency technology for nitrogen and phosphorus removal, applied in the direction of bacteria, water/sludge/sewage treatment, biochemical equipment and methods, etc., can solve the problems of low treatment cost, increased infrastructure cost, high cost, etc., to achieve low treatment cost, The effect of good nitrogen and phosphorus removal ability
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[0015] Embodiments of the present invention are described below
[0016] 1. Isolation and purification of strains
[0017] Take 10mL of sludge sample and place it in a 250mL Erlenmeyer flask containing 100mL of sterile water, add some glass beads, shake at 30°C for 30min. Take 0.5mL of the mixed solution in a test tube filled with 4.5mL sterile water, shake and mix to make a concentration gradient of 10 -1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 , 10 -6 suspension. Take 0.1 mL of different gradient suspensions and spread them on YG plates, make three plates for each dilution, and incubate at 30°C for 2-3 days. Colonies with different shapes were selected from the plate, streaked and purified on the YG plate; the purified bacteria were transferred to LB slant medium, cultured at 30°C for 2 days, and stored at 4°C.
[0018] 2. Screening of strains
[0019] (1) Preliminary screening of blue and white spots: the colonies with different shapes picked out from the plate were spotte...
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