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High-efficiency denitrification and dephosphorization bacterial strain C18

A high-efficiency technology for denitrification and phosphorus removal, applied in bacteria, water/sludge/sewage treatment, biochemical equipment and methods, etc. Phosphorus capacity, the effect of low treatment cost

Inactive Publication Date: 2010-12-22
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

From the perspective of the principle of nitrogen and phosphorus removal, some chemical and physical methods can effectively remove nitrogen and phosphorus in sewage, but in general, the operation of these two methods is relatively complicated, the cost is high, and additional structures are required In order to ensure the treatment effect and increase the cost of capital construction, the biological treatment of nitrogen and phosphorus removal has the advantages of simultaneously removing C, N, P and low treatment cost.

Method used

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  • High-efficiency denitrification and dephosphorization bacterial strain C18
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  • High-efficiency denitrification and dephosphorization bacterial strain C18

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Experimental program
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Embodiment Construction

[0015] Embodiments of the present invention are described below

[0016] 1. Isolation and purification of strains

[0017] Take 10mL of sludge sample and place it in a 250mL Erlenmeyer flask containing 100mL of sterile water, add some glass beads, shake at 30°C for 30min. Take 0.5mL of the mixed solution in a test tube filled with 4.5mL sterile water, shake and mix to make a concentration gradient of 10 -1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 , 10 -6 suspension. Take 0.1 mL of different gradient suspensions and spread them on YG plates, make three plates for each dilution, and incubate at 30°C for 2-3 days. Colonies with different shapes were selected from the plate, streaked and purified on the YG plate; the purified bacteria were transferred to LB slant medium, cultured at 30°C for 2 days, and stored at 4°C.

[0018] 2. Screening of strains

[0019] (1) Preliminary screening of blue and white spots: the colonies with different shapes picked out from the plate were spotte...

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Abstract

The invention provides a high-efficiency denitrification and dephosphorization bacteria strain C18, which belongs to the technical field of waste water biochemical treatment process. The bacterial strain C18 is gram-negative bacteria, which is identified as a pseudomonas (pseudomonas grimontii). The main biological characteristic of the bacterial strain is G-; thalli is of short rod shape; the size is about 0.9*0.6 mu m; a flagellum is grown on a partial side; V-P test is positive; indole test is positive; and the bacterial strain can hydrolyze starch. After 24 hours anaerobic cultivation, the bacterial strain C18 has a dephophorization rate of 88.3 percent and a denitrogenation rate of 83.4 percent, and can be used in the waste water biochemical treatment process so as to reduce the content of the nitrogen and the phosphorus in the waste water greatly.

Description

1. Technical field [0001] The invention relates to a high-efficiency denitrification and phosphorus removal bacterium C18, which uses microorganisms to improve the efficiency of waste water denitrification and phosphorus removal technology, and is suitable for the fields of water pollution control and waste water biochemical treatment technology. 2. Background technology [0002] For a long time, the purpose of sewage treatment has focused on the removal of organic matter and suspended solids, without considering the removal of nitrogen and phosphorus. With the increase of sewage discharge, especially the widespread use of chemical fertilizers, pesticides, and synthetic detergents, the impact of nitrogen and phosphorus nutrients in sewage on the environment has gradually attracted people's attention. The most prominent impact of the discharge of nitrogen and phosphorus plant trophic pollutants on the water environment is the eutrophication of the water body, which is manifes...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/38
Inventor 蔡天明陈立伟蔡舒吴守中李晓丹刘志伟
Owner NANJING AGRICULTURAL UNIVERSITY
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