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Tolumonas osonensis bacterial strain with electrogenesis characteristic, and applications thereof in microbial fuel cells

A fuel cell and microorganism technology, applied in the biological field, can solve the problem of no relevant reports on the characteristics of electricity production, and achieve the effect of high electricity production and activity

Inactive Publication Date: 2014-01-15
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, no relevant reports have been found at home and abroad on the electricity production characteristics of Tolumonas osonensis microorganisms and their application in microbial fuel cells

Method used

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  • Tolumonas osonensis bacterial strain with electrogenesis characteristic, and applications thereof in microbial fuel cells
  • Tolumonas osonensis bacterial strain with electrogenesis characteristic, and applications thereof in microbial fuel cells
  • Tolumonas osonensis bacterial strain with electrogenesis characteristic, and applications thereof in microbial fuel cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: Separation and purification of microorganisms on MFCs anode biofilm

[0018] Take the sludge from the sludge concentration room of Tianjin Taida Sewage Treatment Plant, and after simple elutriation and sedimentation treatment, remove the inorganic sediment at the bottom and the clear water on the surface, and take the sludge with a moisture content of about 95% in the middle as the inoculum , and sludge nutrient solution (sludge 10mL, mineral solution 625μL, vitamin solution 250μL, 100mM PBS buffer solution 5mL, glucose 0.05g, add distilled water to 50mL.) into the single-chamber MFCs reactor. The main body of the reactor is a plexiglass cylinder with a length of 2.0cm and a cross-sectional diameter of 3.0cm. The effective volume is 14mL, and the effective area of ​​the cathode and anode is 7cm. 2 . The anode and cathode are respectively placed on the two sides of the MFCs main body, and sealed and fixed with rubber rings. One end of the anode is covered...

Embodiment 2

[0019] Example 2: Analysis of the electricity production performance of pure microorganisms on the MFCs anode biofilm

[0020] Pick each isolate that embodiment 1 obtains, inoculate into iron citrate respectively as electron donor (final concentration is 5.000g / L), glucose is as substrate (final concentration is 1.000g / L) in the PBBM liquid medium , 22°C, under aerobic conditions, static culture for 48h. The culture solution of each isolate was centrifuged at 6000r / min for 20min at 4°C to collect the bacterial cells.

[0021] The thalli of the same quality of different isolates were inoculated in anolyte 1 (PBBM liquid medium was added ferric citrate as electron donor (final concentration is 5.000g / L), glucose was used as substrate (final concentration was 1.000g / L) respectively. / L), pH=7.2, conductivity is 7.8mS / cm.), the inoculum solution obtained is injected in the MFCs reactor, and the reactor is connected with the external resistance 1000Ω and the data acquisition devic...

Embodiment 3

[0025] Example 3: 16S rDNA sequencing analysis of bacterial strain P2-A-1

[0026] The bacterial genomic DNA extraction kit was used to extract the genomic DNA of strain P2-A-1 as a template, and the universal primer (27F, 1492R) of bacterial 16Sr DNA was used as a primer to amplify the 16S rDNA fragment of strain P2-A-1. The 16S rDNA gene of the strain has the nucleotide sequence shown in the sequence table, and the sequence length is 1425bp. The obtained 16S rDNA gene sequence was input into GenBank, and all the sequences in the database were compared and analyzed by the Blast program. It was found that the The 16S rDNA gene sequence of the invention P2-A-1 strain and the strain Tolumonas osonensisOCF7 of the genus Tolumonas (Tolumonas) T It has a high similarity (the accession number on Genbank is GU370947), and the homology reaches 99%.

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Abstract

The invention relates to Tolumonas osonensis P2-A-1 bacterial strain with electrogenesis characteristic. The bacterial strain is obtained by steps of enriching, separating, purifying and screening from sludge of sewage treatment plant; belongs to Tolumonas osonensis species, Tolumonas genus; and is named as Tolumonas osonensis P2-A-1. The bacterial strain is preserved in China General Microbiological Culture Collection Center, on May, 2013, and the preservation number is CGMCC No.7562. The bacterial strain possesses following characteristics: short rod-liked mycelial morphology; 2.4 to 3.6 [mu]m in length, and 0.9 [mu]m in width; the bacterial strain is distributed separately or in pairs, possesses no flagellum, is capable of growing with or without oxygen; no bacterial spore is produced; gram negative; at a temperature of 22 DEG C, after 48h of culturing on tryptone soybean broth plating medium, white bacterial colonies are formed, are round in shape, are easy to be stirred up, and are nontransparent, the edge is smooth, and diameter is 2 to 4mm; the bacterial strain is capable of using a plurality of substrates such as glucose and sodium acetate. The bacterial is used for electrogenesis in microbial fuel cells, electrogenesis capacity is relatively high, and power density reaches 509mW / m<2>. The electrogenesis capacity of T.osonensisa, and applications of T.osonensisa in microbial fuel cells are disclosed for the first time.

Description

technical field [0001] The invention belongs to the field of biotechnology, and particularly relates to the electricity production characteristics of a Tolumonas osonensis bacterial strain and its application research in microbial fuel cells. Background technique [0002] With the continuous development of society and economy, energy consumption will increase year by year. The continuous increase in consumption of non-renewable energy such as coal, oil, and natural gas has not only caused human beings to face the pressure of resource depletion, but also brought about problems such as global warming and acid rain. Therefore, in order to maintain a stable and sustainable supply of human energy and achieve sustainable economic growth, changing the way of energy production and consumption, and developing new, environmentally friendly, and non-petroleum renewable energy supply methods are the main body of future energy development. train of thought. [0003] Microbial Fuel Cell...

Claims

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Application Information

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IPC IPC(8): C12N1/20H01M8/16C12R1/01
CPCY02E60/50
Inventor 骆健美周明华杨佳王敏
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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