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Detecting method for pesticide residue in fruits

A technology for pesticide residues and detection methods, applied in the field of food analysis, can solve the problems of acute or chronic poisoning and frequent poisoning of humans and animals, and achieve the effects of avoiding interference, simple processing operations and high extraction efficiency.

Inactive Publication Date: 2015-10-07
DONGCHEN LEADER TESTING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The role of organophosphorus pesticides (OPs) in ensuring crop yields cannot be ignored. However, organophosphorus pesticides, which have been used in large quantities for a long time, remain on grains and vegetables in small or trace amounts, which can easily cause acute or chronic poisoning
Poisoning incidents caused by organophosphorus pesticides are more frequent and serious than any other pesticides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The detection method of pesticide residues in fruits includes the following steps:

[0019] Step 1. Take apples, crush and homogenize;

[0020] Step 2. Add the crushed and homogenized fruit sample in step 1 to deionized water for soaking, add hydroquinone and sodium thiosulfate, and homogenize;

[0021] Step 3. Centrifuge the homogenized solution of step 2 to remove the precipitate;

[0022] Step 4. Add cholinesterase and 2,6-dichloroindophenol acetate to the supernatant obtained in step 3.

[0023] The volume ratio of fruit sample and deionized water in step 2 is 5:62.

[0024] In step 2, the amount of hydroquinone is 0.1 g / mL, and the amount of sodium thiosulfate is 0.2 g / mL.

[0025] The homogenization conditions in step 2 are 12000rpm and 15min.

[0026] The centrifugation conditions in step 3 are 0°C, 3000 rpm, and 15 min.

[0027] Step 4 is charging CO 2 Add cholinesterase and 2,6-dichloroindophenol acetate under the same conditions.

Embodiment 2

[0029] The detection method of pesticide residues in fruits includes the following steps:

[0030] Step 1. Take the golden pear and crush the homogenate;

[0031] Step 2. Add the crushed and homogenized fruit sample in step 1 to deionized water for soaking, add hydroquinone and sodium thiosulfate, and homogenize;

[0032] Step 3. Centrifuge the homogenized solution of step 2 to remove the precipitate;

[0033] Step 4. Add cholinesterase and 2,6-dichloroindophenol acetate to the supernatant obtained in step 3.

[0034] The volume ratio of fruit sample and deionized water in step 2 is 7:50.

[0035] The dosage of hydroquinone in step 2 is 0.2 g / mL, and the dosage of sodium thiosulfate is 0.6 g / mL.

[0036] The homogenization conditions in step 2 are 14000rpm and 12min.

[0037] The centrifugation conditions in step 3 were 0°C, 4000 rpm, and 10 min.

[0038] Step 4 is charging CO 2 Add cholinesterase and 2,6-dichloroindophenol acetate under the same conditions.

Embodiment 3

[0040] The detection method of pesticide residues in fruits includes the following steps:

[0041] Step 1. Take bananas, crush and homogenize;

[0042] Step 2. Add the crushed and homogenized fruit sample in step 1 to deionized water for soaking, add hydroquinone and sodium thiosulfate, and homogenize;

[0043] Step 3. Centrifuge the homogenized solution of step 2 to remove the precipitate;

[0044] Step 4. Add cholinesterase and 2,6-dichloroindophenol acetate to the supernatant obtained in step 3.

[0045] The volume ratio of fruit sample and deionized water in step 2 is 10:75.

[0046] In step 2, the amount of hydroquinone is 0.1 g / mL, and the amount of sodium thiosulfate is 0.2 g / mL.

[0047] The homogenization conditions in step 2 are 14000rpm and 11min.

[0048] The centrifugation conditions in step 3 are 0°C, 4500 rpm, 12 min.

[0049] Step 4 is charging CO 2 Add cholinesterase and 2,6-dichloroindophenol acetate under the same conditions.

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Abstract

The invention provides a detecting method for pesticide residue in fruits. The method comprises the following steps that a fruit sample is selected, and smashing and homogenating are carried out; after the smashing and the homogenating are carried out, the fruit sample is added into deionized water to be subjected to soaking, hydroquinone and sodium thiosulfate are added, and homogenizing is carried out; after the homogenizing is carried out, a solution is subjected to centrifuging, and sediments are removed; cholinesterase and 2,6-dichloro indophenol acetate are added into obtained supernatant. The detecting method is simple in processing and operating, high in extracting efficiency, capable of avoiding heavy metal ions and the reaction temperature interfere a detecting result, and widely applied to detecting the pesticide residue in the various fruits.

Description

Technical field [0001] The invention belongs to the technical field of food analysis, and specifically relates to a method for detecting pesticide residues in fruits. Background technique [0002] my country is a large agricultural country, and agriculture plays a pivotal role in the national economy and people's lives. Crop diseases, insect pests, weeds, rodents, etc. are the main disasters that affect agricultural production, and chemical pesticides are used in the prevention and control of crop diseases, pests, weeds, and rodents due to their quick effects, wide control range, stable properties, easy storage and transportation, and low prices. It plays a huge role in eliminating health pests and diseases. It is an indispensable and important means of production in modern agricultural production and promotes the development of modern agriculture. [0003] Pesticide Residue is a general term for trace pesticide progenitors, toxic metabolites, degradation products and impuriti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/34G01N21/78
Inventor 熊开胜张海防谢建庭
Owner DONGCHEN LEADER TESTING
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