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Method for determining ammonium nitrogen, nitrate nitrogen, nitrite nitrogen and total dissolved nitrogen in sample

A method of determination, ammonium nitrogen technology, applied in color/spectral characteristic measurement, material analysis by observing the influence of chemical indicators, analysis by making materials undergo chemical reactions, etc., can solve the speed of limited reactions and Uniformity, detection limit and accuracy need to be improved to achieve the effect of low detection limit, high accuracy and high sensitivity

Pending Publication Date: 2022-04-15
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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Problems solved by technology

[0003] The method for measuring nitrogen content in soil in the prior art mainly relies on ordinary UV-visible spectrophotometers, which can only measure a limited number of samples each time
Moreover, limited by the speed and uniformity of the reaction during the assay, it cannot be realized on a 96-well plate (microplate reader).
In addition, relying solely on ordinary UV-visible spectrophotometers for measurement, the detection limit and accuracy need to be improved

Method used

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  • Method for determining ammonium nitrogen, nitrate nitrogen, nitrite nitrogen and total dissolved nitrogen in sample
  • Method for determining ammonium nitrogen, nitrate nitrogen, nitrite nitrogen and total dissolved nitrogen in sample
  • Method for determining ammonium nitrogen, nitrate nitrogen, nitrite nitrogen and total dissolved nitrogen in sample

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preparation example Construction

[0043] In the present invention, the mass ratio of sodium hypochlorite to sodium chloroisocyanurate in the oxidizing agent is preferably 6.5-7.2:0.025-0.035. In a specific embodiment of the present invention, the preparation method of the oxidizing agent preferably includes the following steps: measure 65mL of sodium hypochlorite solution (available chlorine content > 5.3%), and dissolve it in 1000mL of water; Sodium isocyanurate, standing overnight, yields the oxidizing agent.

[0044]In a specific embodiment of the present invention, the preparation method of the color developer preferably includes the following steps: weighing 144 g of sodium salicylate and dissolving it in 1000 mL of NaOH solution with a concentration of 0.3 mol / L to obtain the color developer.

[0045] In the present invention, the order of mixing the ammonium nitrogen standard solution, the oxidizing agent and the color developing agent is preferably that the ammonium nitrogen standard solution is mixed ...

Embodiment 1

[0094] Preparation of soil solution: Take fresh soil and pass it through a 2mm sieve, weigh 0.5g of the sieved soil (fresh weight), add 5mL of K with a concentration of 0.5mol / L 2 SO 4 Aqueous solution, shake and extract at room temperature for 1h, pass through a 0.45μm filter membrane, and take the filtrate as soil solution; divide the soil solution into four groups for the determination of ammonium nitrogen, nitrate nitrogen, nitrite nitrogen and total dissolved nitrogen When measuring, the volume of every part of the soil solution is 1mL.

[0095] (1) Establishment of ammonium nitrogen concentration-absorbance standard curve

[0096] Preparation of oxidizing agent: measure 65mL of sodium hypochlorite solution (available chlorine content > 5.3%) and dissolve it in 1000mL of water; under stirring conditions, slowly add 0.025g of sodium chloroisocyanurate and let stand overnight to obtain the oxidizing agent.

[0097] Preparation of color developer: Weigh 144 g of sodium sal...

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Abstract

The invention provides a method for determining ammonium nitrogen, nitrate nitrogen, nitrite nitrogen and total dissolved nitrogen in a sample, and belongs to the technical field of nitrogen content determination. When the ammonium nitrogen is measured, an oxidizing agent (sodium hypochlorite and sodium chloro isocyanurate) and color developing agent (salicylic acid) system is adopted, the ammonium nitrogen is oxidized into monochloramine under the action of the oxidizing agent, the formed monochloramine and the color developing agent form indophenol, and measurement is carried out by measuring the ultraviolet absorbance at the position of 660 nm; when nitrate nitrogen, nitrite nitrogen and total dissolved nitrogen are measured, a new first Griess reagent (N-(1-naphthyl) ethylenediamine dihydrochloride) and a second Griess reagent system (sulfanilamide and vanadium chloride) are adopted for measurement, the detection limit is low, and the sensitivity is high. The chromogenic reaction is stable and uniform in speed and can be carried out on a 96-well plate.

Description

[0001] This application is a divisional case with an application date of August 23, 2019, an application number of 201910782806.3, and an invention title of "A Method for Determination of Ammonium Nitrogen, Nitrate Nitrogen, Nitrite Nitrogen, and Total Dissolved Nitrogen in a Sample" Apply. technical field [0002] The invention relates to the technical field of nitrogen content detection, in particular to a method for measuring ammonium nitrogen, nitrate nitrogen, nitrite nitrogen and total dissolved nitrogen in a sample. Background technique [0003] The method for measuring nitrogen content in soil in the prior art mainly relies on a common ultraviolet-visible spectrophotometer, which can only measure a limited number of samples each time. Moreover, limited by the speed and uniformity of the reaction during the determination, it cannot be realized on a 96-well plate (microplate reader). In addition, relying solely on the ordinary UV-Vis spectrophotometer method for measu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/33
CPCG01N21/78G01N21/33
Inventor 吴星贾永锋
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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