Rapid detection card for detecting pesticide residues, preparation method and application thereof

A technology of pesticide residues and rapid test cards, which is applied in the direction of biochemical equipment and methods, and microbial measurement/inspection, can solve the problems of difficulty in popularization, low sensitivity of acetylcholinesterase, easy inactivation, etc., and achieves wide application range, Achieve real-time observation and easy application

Inactive Publication Date: 2012-04-04
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The application of the above-mentioned several kits or test cards has solved the problem of detecting organic phosphorus and carbamate pesticide residues in agricultural products to a certain extent. Disadvantages such as difficulty

Method used

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  • Rapid detection card for detecting pesticide residues, preparation method and application thereof
  • Rapid detection card for detecting pesticide residues, preparation method and application thereof
  • Rapid detection card for detecting pesticide residues, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Such as figure 1 As shown, it is a schematic structural diagram of the rapid test card for detecting pesticide residues of the present invention, including: a cover film 10 , a first plastic plate 20 , a sample injection hole 30 , a plastic film 40 , a second plastic plate 50 , and a substrate sheet 60 . Wherein, the cover film 10 is attached on the surface of the first plastic plate 20, and the plastic plate 20 is provided with two sample injection holes 30 (spacing 1-1.5cm), and an enzyme sheet (not shown in the figure) is pasted under the sample injection hole 30. marked), the plastic film 40 is placed in the middle of the first plastic plate 20 and the second plastic plate 50, and the second plastic plate 50 is pasted with two substrate sheets 60 corresponding to the enzyme sheet.

[0033] Sample treatment: Randomly select several common vegetables such as Chinese cabbage, celery, lettuce, pepper, cucumber and eggplant in the market, rinse with phosphate buffer (0.1...

Embodiment 2

[0036] figure 2 It is another structural schematic diagram of the quick test card for detecting pesticide residues of the present invention. its assembly with figure 1 The same, except that the first plastic plate 20 is provided with three sample injection holes 30 , and the corresponding second plastic plate 50 is affixed with three substrate sheets 60 . The method of use is the same as in Example 1, so two samples can be detected simultaneously.

Embodiment 3

[0038] The cellulose acetate membrane was cut into two small discs with diameters of 0.8 cm and 1.0 cm, respectively, for making enzyme sheets and substrate sheets.

[0039] At 4°C, mix housefly acetylcholinesterase, glutaraldehyde, and bovine serum albumin at a ratio of 3 U / tablet, 0.2 μl / sheet, and 1 μl / sheet with 4 ml of phosphate buffer, and add a cellulose acetate membrane with a diameter of 0.8 cm. After soaking for 15 minutes, take it out and place it in a small fume hood to dry in the shade, which is the enzyme tablet.

[0040] When making the substrate sheet, dissolve acetyl indophenol into acetone at a concentration of 400mg / L, add a cellulose acetate film with a diameter of 1.0cm, soak for 15 minutes, take it out and place it in a small fume hood to dry in the shade, which is the substrate sheet .

[0041] Shape the plastic sheet into two rectangles 4cm long and 2cm wide. Wherein, a hole is punched on the first plastic plate, the diameter of the hole is 0.5 cm, and ...

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PUM

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Abstract

The invention relates to a rapid detection card for detecting pesticide residues, a preparation method and application thereof. The fast detection card comprises a coverage film, a first plastic board, sample loading holes, an enzyme tablet, a plastic film, a second plastic board and a substrate sheet, wherein the coverage film is adhered to the surface of the first plastic board; at least two sample loading holes are formed in the first plastic board; the enzyme tablet fixed with housefly acetylcholinesterase is adhered to the reverse sides of the sample loading holes and is opposite to the sample loading holes; the substrate sheet, which is fixed with acetyl indophenol and is adhered to the position corresponding to the enzyme tablet, is arranged on the second plastic board; and the plastic film is arranged in the middles of the first plastic board and the second plastic board. In the rapid detection card, enzyme is the housefly acetylcholinesterase which is subjected to molecular evolution, so the rapid detection card has the advantages of high activity, high sensitivity, high stability and the like and can be applied to the rapid detection of organophosphorus and carbamic acid ester pesticide residues in agricultural products, food and soil and particularly vegetables, fruits.

Description

technical field [0001] The invention belongs to the field of food safety, and relates to a quick test card for detecting pesticide residues and a preparation method and application thereof, in particular to a quick test card for detecting pesticide residues and a preparation method thereof, and the quick test card is used in organophosphorus and amino acids. Application in rapid detection of formate pesticide residues. Background technique [0002] In recent years, food safety issues have seriously threatened human health, and the pesticide residues and acute and chronic poisoning in fruits and vegetables caused by excessive use or abuse of pesticides have attracted great attention from the society. In December 2008 and February 2009, Greenpeace randomly selected common fruits and vegetables in the current season in many supermarkets and farmers' markets in Beijing, Shanghai and Guangzhou. Tested. The results showed that not only most of the vegetables and fruits had pesti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/44
Inventor 唐雪明谭芙蓉王利刚赵凯王金斌吴潇朱宏蒋玲曦陶世如
Owner SHANGHAI ACAD OF AGRI SCI
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