265results about How to "The result is easy to judge" patented technology

The invention discloses a test strip which is used for testing porcine viral diarrhea pathogen in one step, and the test strip comprises a support layer, an adsorption layer and a protection layer. The adsorption layer has a sample fiber layer, a gold antibody fiber layer, a cellulose film layer and a hygroscopic material layer at the handle end sequentially from a test end. The cellulose film layer has a test blot and a contrast blot, and the gold antibody fiber layer is adhered with at least one of monoclonal antibodies or polyclonal antibodies of anti-TGEV, anti-PDEV and anti-RV with colloid gold mark; the test blot is printed by at least one of polyclonal antibody liquid or monoclonal antibody liquid of anti-TGEV, anti-PDEV and anti-RV, and the contrast blot is printed by IgG solution of sheep or rabbit anti-mouse or IgG solution of sheet or rabbit anti-porcine. When used for testing, the test strip has the advantages of strong specificity, high sensitivity, convenient and rapid operation and direct and accurate testing result. The test strip is especially suitable for fast identifying and diagnosing on site.

A 3D grid detection technique for detecting object nuclein and object protein includes requiring to add hybridized protein or hybridized probe on one of grid component when object nuclein or object protein is detected then coordinating by immobilized catch-protein or catch-probe to realize specificity-catch of object nuclein or object protein.

The invention provides a preparing and application method of diffusion medium box for diagnosing livestock's snail fever spot gold, which is a new immune labeling technique and livestock's snail fever immunology diagnosis technique. The invention labels colloid gold onto Japanese snail antigen protein, and is suit for testing many kinds of animals such as cattle, sheep, pig and rabbit; it spots tested animal's blood dried paper leachate onto pyroxylin film as posited phase, then it droplets snail antibody colloid gold 2í½3 drops (100í½150ª–l) and produces naked eye visible red speckle in 2 minutes. Its positive coincidence rate is 100% and its negative coincidence rate is 99.2%.

The invention discloses a preparation method of immunogold rapid test paper for vibrio parahaemolyticus. The method comprises the following steps of: (a) preparing a vibrio parahaemolyticus antigen; (b) preparing a vibrio parahaemolyticus inactivated vaccine; (c) preparing a polyvalent antiserum with a vaccine immune experimental rabbit; (d) purifying the antiserum; (e) marking with colloidal gold; and (f) preparing an immunogold test strip. A detection technology in which the immunogold rapid test paper for vibrio parahaemolyticus is adopted has the advantages of easiness, sensitivity, rapidness, specificity and the like; the customs clearance speed of port imported and exported goods can be increased greatly; and meanwhile, the test paper has the advantages of low price, no need of instrument, easiness and rapidness for operationing and easiness in judging results.

The invention discloses a detection method for clostridium perfringens, a primer group and a detection kit for same. The primer group is based on the Loop-Mediated Isothermal Amplification (LAMP), and is obtained through analysis, design and artificial synthesis on the special alpha toxin (C. perfringens alpha toxin, CPa) gene sequence of the clostridium perfringens, the contained nucleotide sequence is shown in SEQ No.1-4, and the primer group has considerably high specificity to the clostridium perfringens. The fast detection method for the clostridium perfringens carries out LAMP reaction on the DNA of the clostridium perfringens in a sample by utilizing the detection primer group, and whether the clostridium perfringens is contained in the sample or not is judged by identifying reaction products. The invention also designs the fast detection kit for the clostridium perfringens according to the detection method, so that fast, simple, accurate and efficient clostridium perfringens detection and identification can be carried out on the sample.

The invention relates to a method and system for transcribing and applying a test script on a mobile terminal. The method comprises the following steps: the mobile terminal is connected to a computer to start up an application program in a debug mode, and a debugger program and the application program on the computer are connected with each other through a TCP (Transmission Control Protocol) / IP (Internet Protocol); an application source code is not needed, and a breaking point is added in correlation functions operated by a user in the environment of a black box; the user operates on the mobile terminal and triggers breaking point hit; at the moment, the debugger program records related actions and transcribes operation aiming at an interface element, requests obtaining the current variable value through a debug protocol at the same time, and continues execution in the breaking point position not to influence the operation of the user; and finally the test script is formed. According to the technical scheme, the script can be simply and conveniently transcribed, meanwhile, the degree of accuracy of script execution is high, and the result is easily judged.

The present invention discloses a cucumber variety identification method based on single nucleotide polymorphism markers, wherein 12 single nucleotide polymorphism markers of cucumber are subjected to genotyping analysis by using a pyrosequencing technology so as to achieve authenticity identification on the cucumber variety. With the detection method of the present invention, the cucumber variety authenticity identification can be completed within 5 h, and characteristics of stability, high-throughput, accuracy and the like are provided.

The invention discloses a homologous recombination defect judgment method based on DNA sequencing data. The method comprises the following steps: acquiring characteristic attributes; extracting validdata; based on a triple learning method framework, selecting three different base classifiers H1, H2 and H3 by considering good generalization ability, high accuracy and processing efficiency for multi-dimensional feature attributes; performing iterative training on the H1, H2 and H3 to obtain an extended training set, and updating a model to complete a training process; and marking a unmarked sample set U by using the trained model, and completing judgment of the HRD state according to the marking result. According to the method, the limitation that HRD state judgment is carried out by usinglocal characteristics such as a single genome instability state or a small number of genome instability states and the like is solved, the difficulty that the number of samples with clinically known HRD states is extremely small is overcome, learning of multi-characteristic attributes under existing sample data is achieved, and the performance of the HRD judgment method can be improved.

The invention discloses a test strip that is used for detecting antibodies of porcine parvovirus and porcine encephalitis B virus in one step, which comprises a supporting layer, an adsorptive layer and a protecting layer, and the adsorptive layer consists of a sample fiber layer, an adsorptive gold mark fiber layer, a fibrin membranous layer and a water absorptive material layer sequentially from a testing end; the adsorptive gold mark fiber layer adsorbs any one or two from antigen liquids of gold colloid marked SPA or gold colloid marked pure PPV and JEV; a detecting print and a contrast print are arranged on the fibrin membranous layer, the detecting print is printed with any one or two from the purified PPV and JEV antigen liquids, and the contrast print is printed with an IgG solution of ovine or rabbit anti-SPA or an IgG solution of any one or two viruses from ovine or rabbit anti-PPV and anti-JEV. The test strip of the invention has strong detecting specificity and high sensitivity, avoids additional equipment and reagents, can be operated by every person, and is particularly applicable to the quick specific antibody detection of porcine diseases on the spot.

The invention discloses a Cronobacter sakazakii strain detecting method and kit. The method comprises the following steps of: (1) extracting genome DNA (Deoxyribose Nucleic Acid) of a sample to be detected and carrying out PCR (Polymerase Chain Reaction) amplification by taking the genome DNA as a template and a Cronobacter sakazakii specific amplification primer pair as a primers, wherein one primer in the primer pair has the sequence shown in SEQ ID NO.1, and the other primer has the sequence shown in SEQ ID NO.2; and (2) detecting the existence of a single amplification product at a 438bp position. The method provided by the invention is short in Cronobacter sakazakii strain detecting time, capable of reducing the detecting cost and increasing the detecting efficiency, single in specificity, reliable in detection result and simple in result judgment. The invention provides a simple, rapid and sensitive Cronobacter sakazakii strain detecting method for the technical field of food safety detection, which has greater significance for food safety in China.

The invention discloses a method for fast appraising the purity of cucumber hybrid seed, which comprises the following steps of: taking the genome DNA of a 'Jingyou 38' test seed of a new product of the room temperature cucumber as a template, informing to analyze the differentiation segment of parent DNA of the known 'Jingyou 38', and appraising the purity of the 'Jingyou 38' hybrid seed by applying a pyrophosphoric acid sequencing technology. The method can appraise the purity of the seed within 3h, has the characteristics of being simple and easy to operate, high in sensitivity, fast, low in cost, convenient for popularization and the like, and developing a wide foreground for appraising the purity of the seed.

The invention discloses a loop-mediated isothermal amplification (LAMP) primer of a genetically modified maize BT11 strain, a reagent kit and application of the LAMP primer. The primer used for the LAMP detection has base sequences being SEQ ID NO:2-7, the LAMP detection technology is adopted, a turbidity method or a color development method is combined, and a standard method applicable to the specificity detection of the genetically modified maize BT11 strain in the food is built. The method has the advantages that the operation is simple, the dependence on expensive instruments is avoided, the detection time is about one hour, the sensitivity of the method reaches 0.5 percent, the detection efficiency is the same as that of the traditional PCR (polymerase chain reaction) method or a fluorescent quantification PCR method, and the method can be applied to inspection and quarantine practical work, is particularly applied to the fast preliminary screening of a plurality of samples and the food quality monitoring of various kinds of basic laboratories and is suitable for being used as industrial recommended standards to be applied and popularized.

The invention discloses a test reagent for realizing the online rapid nondestructive test of the quality of a gold plating layer and a using method of the test reagent. The reagent contains trifluoroacetic acid and copper sulfate pentahydrate. The using method comprises the following steps: dropwise adding 2-3 drops of test reagent in a to-be-detected region of the gold plating layer, wiping the gold plating layer by using dust-free cloth after 60-120s, and observing the color change condition of the gold plating layer; if the color of the gold plating layer is changed, judging that a workpiece is a defective product; if the color of the gold plating layer is unchanged, judging that the plating layer is qualified. The test reagent disclosed by the invention is rapid in reaction, an obvious color difference exists between a generated corrosion product and the plating layer, and a result is high in accuracy and easy to judge; the test reagent is extremely simple in operation, no special instruments are needed, and online detection can be realized; the test reagent can be used for realizing nondestructive test, and workpieces which are qualified through test can be continued to be processed, assembled and circulated on a production line, so that the resource is saved; the test reagent is low in cost but capable of accurately reminding a manufacturer of stopping the subsequent processing of a defective product of the plating layer and taking remediation measures in time, so that more loss is avoided.

The invention provides an N gene specific primer pair, a method and a kit for detecting resistance of tobacco to a TMV. The specific primer pair comprises an upstream primer and a downstream primer, wherein the nucleotide sequence of the upstream primer is as shown in SEQ ID NO:1, and the nucleotide sequence of the downstream primer is as shown in SEQ ID NO:2. The method comprises the following steps: designing a pair of specific primers in a tobacco N gene sequence specific zone; with the DNA of a to-be-detected tobacco variety as a template, performing PCR amplification on the N gene specific primer pair; and detecting a PCR amplification product by electrophoresis and judging whether the PCR amplification product contains a 865bp characteristic band, wherein if so, the tobacco has resistance to TMV, and otherwise, the tobacco does not have N gene mediated resistance to TMV. The method has the advantages of reliable result, high detection speed and the like and is simple to operate, the breeding process of TMV-resisting breeding materials can be greatly accelerated, the breeding period is shortened and the breeding efficiency is enhanced.

The invention discloses a method for quickly and nondestructively distinguishing between real and fake bird's nests. The method is characterized in that Raman spectrometry is adopted; pretreatment is not required to be performed on samples; technical parameters are directly set; Raman spectra is collected through a Raman spectrometer; whether characteristic peaks of bird's nests appear or not is found and the quantity is determined. If 3 to 6 characteristic peaks of bird's nests appear and other obvious impure peaks do not exist, the samples are manifested to be real bird's nests; if 3 to 6 characteristic peaks of bird's nests appear and other obvious impure peaks exist, the samples are manifested to be fake bird's nests; the adulterate amount is roughly judged according to peak height ratio of the impure peaks and the characteristic peaks of the bird's nests, the higher the ratio is, the lower the bird's nest content is; if 0 to 2 characteristic peaks of the bird's nests appear, the samples do not contain bird's nest components. The method has the characteristics that the samples are not required to be destroyed, the method is simple to operate, discrimination speed is high, the accuracy is high, and the like, is applied to the discrimination of large-scale bird's nest samples, and is novel.

The test strip for fast detecting colibacillus O157:H7 antigen belongs to the field of medical diagnosis article technology. The fast test strip includes one base substrate, one fiber film covering the base substrate, one water absorbing film covering the upper part of the base substrate, monoclonal marking antibody of colibacillus O157:H7 antigen adsorbed onto the fiber film in the lower part of the test strip, one anti-mouse IgG coated quality controlling line on the middle part of the fiber film, and one detecting line coated with monoclonal antibody of colibacillus O157:H7 antigen with excellent pairing property to absorbed marking antibody below the quality controlling line. The marking antibody has marker of colloidal gold, the concentration of colloidal gold antibody is (1.0-3.0)x10<->3 mg / sq cm, and that of the coating antibody is (2.0-8.0)x10<->4 mg / sq cm. The present invention has high detection speed and high detection accuracy.

The invention relates to a rapid detection card for detecting pesticide residues, a preparation method and application thereof. The fast detection card comprises a coverage film, a first plastic board, sample loading holes, an enzyme tablet, a plastic film, a second plastic board and a substrate sheet, wherein the coverage film is adhered to the surface of the first plastic board; at least two sample loading holes are formed in the first plastic board; the enzyme tablet fixed with housefly acetylcholinesterase is adhered to the reverse sides of the sample loading holes and is opposite to the sample loading holes; the substrate sheet, which is fixed with acetyl indophenol and is adhered to the position corresponding to the enzyme tablet, is arranged on the second plastic board; and the plastic film is arranged in the middles of the first plastic board and the second plastic board. In the rapid detection card, enzyme is the housefly acetylcholinesterase which is subjected to molecular evolution, so the rapid detection card has the advantages of high activity, high sensitivity, high stability and the like and can be applied to the rapid detection of organophosphorus and carbamic acid ester pesticide residues in agricultural products, food and soil and particularly vegetables, fruits.

The invention discloses a loop-mediated isothermal amplification (LAMP) detection method for identifying beef and mutton, falling into the field of molecular genetics. The method comprises searching in GenBank to obtain cattle species-specific conserved sequence designed primers, and performing specific screening on designed primers by using LAMP real-time turbidometer; preparing LAMP reaction solution to construct detection system; taking 1muL extracted genome DNA and 1muL fluorescence detection reagent, adding into the LAMP reaction solution to perform LAMP, and identifying according to color of reaction solution, wherein if reaction solution turns into green, the sample contains beef or mutton. The method has convenient and precise detection, easy preparation of templates and primers, high specificity and sensitivity, naked-eye observation of detection result, and wide application prospect; and can satisfy detection requirements of each detection department.

The invention discloses a dual-PCR assay kit for duck circovirus and duck hepatitis virus. The dual-PCR assay kit provides a primer group for assaying duck circovirus and duck hepatitis virus, which consists of a primer 1, a primer 2, a primer 3 and a primer 4; and the nucleotide sequences of the primer 1, the primer 2, the primer 3 and the primer 4 are sequence 1, sequence 2, sequence 3 and sequence 4 sequentially in a sequence table. The dual-PCR technique which can simultaneously assay and identify DuCV (duck circovirus) and DHV (duck hepatitis virus) as two pathogens by establishing PCR reaction just once has good specificity, moreover, the experiment utilizes the difference of amplified fragment lengths to directly determine an amplification result in the primer design, and thereby the method is simpler, more visual and more practical during result determination.

The invention provides an eye fundus image judgment method and eye fundus image judgment equipment. The eye fundus image judgment method comprises the following steps: acquiring a to-be-judged eye fundus image of a user; Obtaining a reference fundus image associated with the fundus image to be judged, wherein the reference fundus image is a fundus image with a known state being normal; Generatinga difference image according to the to-be-judged fundus image and the reference fundus image; And taking the differential image as input data of a machine learning model, enabling the machine learningmodel to output a judgment result of the to-be-judged fundus image, and training the machine learning model by using the training differential image and corresponding label data.

The invention relates to a multiple-gene detecting kit related to antitumor drugs. The multiple-gene detecting kit comprises a mixture of RT (reverse transcription) primers and a mixture of PCR (polymerase chain reaction) amplification primers, wherein each RT primer and each PCR amplification primer based on genetic groups, reference genes and a reaction internal label are respectively contained in each of the mixture of the RT primers and the mixture of the PCR amplification primers; the multiple-gene detecting kit is characterized in that the genetic groups comprise PTEN, EGFR, DPYD, HER2, RRM1, ERCC1, TUBB3, TOP1, TYMS and TOP2A; the reference genes comprise ACTB, GAPDH and B2M; the reaction internal label is KAN-rRNA (ribosomal RNA). The multiple-gene detecting kit related to antitumor drugs disclosed by the invention can be used for systemically detecting the expression level of a plurality of genes closely related to the antitumor drugs by one step, is simple and convenient to use, good in accuracy and high in detecting efficiency, and can be used for guiding chemotherapy drugs and selecting chemotherapy regimens very well.

The invention provides a preparation method of a visualized immunoaffinity gel detection column for rapidly detecting enrofloxacin in food. The method comprises the steps of with sepharose gel as a solid vector, coupling an enrofloxacin antibody with sepharose gel activated by cyanogen bromide to prepare an antibody gum serving as a detection layer; coupling an HRP antibody with the sepharose gel activated by cyanogen bromide to prepare an HRP antibody gum serving as a quality control layer; and putting the HRP antibody gum into 1mL of solid-phase extraction column to prepare the immunoaffinity detection column. The invention develops a novel immunoaffinity gel column detection product for rapidly, qualitatively and semi-quantitatively detecting enrofloxacin residues in food, and the detection limit is 5mu g / L. The immunoaffinity gel column detection product has the following outstanding advantages of high specificity, good sensitivity, simple sample pretreatment, short detection consumed time, high accuracy, simple and convenient operation and no assistance of large-size instruments.

The invention relates to a technology for diagnosing a bladder cancer through a molecular biology detection means and particularly provides a method for detecting FGFR3 gene mutation to diagnose the bladder cancer and a kit. The technology comprises the step that exons 7, 10 and 15 of an FGFR3 gene are detected to determine whether mutations exist in the exons or not. A double-blind screen test shows that the positive bladder cancer detection rate of the kit is 91.18%, the false positive rate is 3.23%, and the technology has a good clinical application value.

The invention discloses a chromogenic culture medium for separating and detecting shigella; the culture medium comprises yeast powder, tryptone, soy peptone, sodium chloride, lactose, cane sugar, 2-deoxy-D-ribose, agar powder, beta-glucosaccharase chromogenic substrate, beta-pyranfucosidase chromogenic substrate, beta-galactosidase chromogenic substrate, phenol red, 3# cholate, novobiocin sodium salt, cefsulodin and cefixime; residue is water; the chromogenic culture medium provided by the invention can be used for separating and detecting four species of shigella and has advantages of high specificity, high sensitivity, easy operation, simple result judgment and the like; the chromogenic culture medium is suitable for detecting each sample and has wide application prospect; and detecting effect of the chromogenic culture medium can achieve level of like products in an import R&F company and is superior to inland like products.

The invention discloses an LAMP primer of staphylococcus aureus. The LAMP primer comprises two inner primers (FIP and BIP) and two outer primers (F3 and B3), namely, F3 as shown in the sequence SEQ ID NO:1, B3 as shown in the sequence SEQ ID NO:2, FIP as shown in the sequence SEQ ID NO:3 and BIP as shown in the sequence SEQ ID NO:4. Aiming at the specific nuc gene of the staphylococcus aureus, a group of primers is designed, and a sensitive and specific staphylococcus aureus LAMP detection method is established by using the primers. The experiment shows that the detection sensitivity and the detection limit of the LAMP detection method are 100 times of those of a PCR method; whether green fluorescence is generated or not can be observed with naked eyes so as to confirm whether the reaction is performed, so that the result judgment can be simple, convenient and feasible; compared with a PCR technique, the method disclosed by the invention has the advantages that the time and the labor can be saved, no expensive instruments or tedious electrophoretic analysis is needed, and the application method disclosed by the invention is particularly applicable to use in base levels.

The invention discloses a dye method digital quantitative PCR kit as well as application and an application method thereof. The dye method digital quantitative PCR kit comprises application of circular RNA hsa_circ_0051778 copy number as a detection index in a lung adenocarcinoma and tuberculous pleuritis identification kit according to the circular RNA hsa_circ_0051778 copy number in tuberculouspleuritis being higher than the copy number in the lung adenocarcinoma. The kit comprises a circular RNA hsa_circ_0051778 specific primer, a positive control, a negative control, a special PCR amplification premix solution for dye-method digital PCR as well as sterile deionized water; and the kit is used by virtue of steps of sampling, PCR amplification system preparation, amplification reaction and identification. By adopting the dye method digital quantitative PCR kit, the lung adenocarcinoma and the tuberculous pleuritis can be accurately and rapidly identified. The detection result provesthat the sensitivity of the detection tool can reach about 90 percent or more which is higher than the existing single method used clinically.

The invention discloses an IgM antibody detection agent for toxoplasma, wherein it uses anti-human IgM-mu chain mono-clone antibody to pack the micro-pore plate, marking with the toxoplasma antigen and enzyme-label antigen for toxoplasma antigen and other assist agents to prepare the detection agent. This invention has high sensitivity and well specificity fit to convenient operation.

The invention discloses an olive oil identification method and an ion mobility spectrometry spectrum of an olive oil standard substance. The identification method comprises the following steps: weighing 50 microliters of a sample and taking the sample into a 50mL test tube, adding 5 mL of a n-hexane solution, continuously adding 2mL of a methanol solution of 1 mol / L potassium hydroxide, and carrying out vortex mixing; heating in 40 DEG C water-bath for 30 min and cooling to room temperature; adding 1 mL of deionized water into the test tube, and carrying out vortex mixing; centrifuging at 5000 r / min for 3 min, taking a supernatant into a 10mL test tube, and drying by nitrogen-blow; carrying out redissolution with 2 mL of methanol, sieving with a 0.22micron filter membrane and waiting to be identified by a machine; setting technical parameters, and acquiring a spectrum of the sample through ion mobility spectrometry; and whether the sample is pure olive oil is judged by comparison between ion mobility spectrometry spectra of olive oil and the sample to be tested. The method has characteristics of simple operation, rapid identification, high accuracy and the like, is suitable for determination of large-batch samples, and is a new method for rapidly identifying the authenticity of olive oil.

The invention discloses a kit for detecting non-mucus type klebsiella pneumoniae with high virulence, and belongs to the technical field of biological detection. An ampR gene sequence in a non-mucus type klebsiella pneumoniae genome with high virulence is used as a marker, primers are designed based on the specific sequence, and finally qualitative detection of the non-mucus type klebsiella pneumoniae with high virulence is completed based on a polymerase chain reaction to make up for the situation that a qualitative detection method of klebsiella pneumoniae in the prior art can determine thevirulence of the klebsiella pneumoniae only by judging whether a mucus type exists or not, but cannot distinguish the difference in virulence among non-mucus type strains, so that the clinical application value of the marks and kit for detecting the non-mucus type klebsiella pneumoniae with high virulence and an application method of the kit is ensured.