178results about How to "High labeling rate" patented technology

The invention discloses a colloidal gold chromatography test paper strip for quickly detecting bisphenol A and a preparation method thereof, and belongs to the technical field of detection of biological immunization methods. The detecting test paper strip consists of a lining board, and a sample pad, a gold labeled combining pad, an enveloping film and a water absorbing pad, which are orderly connected on the lining board, wherein the gold labeled combining pad is glass fiber wool adsorbing gold-labeled antibodies of the bisphenol A; and the enveloping film is provided with a linear invisible test line T line which is printed by carrier protein solution coupled by diphenolic acid, and a linear invisible contrast line C line which is printed by sheep anti-rabbit IgG solution, and the two lines are parallel. The test paper strip has strong specificity, high sensibility with the lowest detection limit of 5ppb, simplicity, convenience, quickness and strong timeliness, does not need any other reagents and instruments, can be operated in field, can determine a detection result in 15 minutes after being put into tested sample solution, has vivid, directviewing and accurate result display, saves cost, has wide applicable range and contributes to promotion.

The invention relates to a test paper strip for rapidly detecting traces of chlorothalonil and a preparation method thereof. The test paper strip is characterized in that a supporting layer is used as the bottom layer, an adsorption layer is used as the intermediate layer, a protective layer is fixed on the adsorption layer, the adsorption layer comprises an adsorption fibrous layer, a gold-labeled antibody fibrous layer, a cellulose film and an absorbent material layer at the handle end in order from a test terminal, wherein the cellulose film is provided with detection blots printed by using a chlorothalonil coupling carrier protein solution and control blots printed by using a goat anti-mouse IgG or rabbit anti-mouse IgG (or goat anti-rabbit IgG) antibody solution; the gold-labeled antibody is a colloidal gold labeled chlorothalonil monoclonal antibody or polyclonal antibody; and the chlorothalonil coupling carrier protein is bovine serum albumin, chicken ovalbumin or haemocyanin. The test paper strip disclosed herein has the advantages of strong specificity, high sensitivity, simple, and accurate detection, low cost, wide application scope, and easiness in popularization and application.

The invention relates to an O-type foot-and-mouth disease virus antibody chemiluminescence detection kit. The kit includes an polystyrene board coated by antigen, a monoclonal antibody, a standard substance, a luminescence substrate solution A and a luminescence substrate solution B. The polystyrene board coated by antigen is an opaque polystyrene plate coated by an O-type foot-and-mouth disease virus RE2 recombinant protein; the monoclonal antibody is a horseradish peroxidase labeled O-type foot-and-mouth virus monoclonal antibody; the luminescence substrate solution A comprises a Lumino, hydroxycoumarin, gallic acid, a Tris-Hcl buffer and water; the standard substance is foot-and-mouth virus O-type antibody respectively diluted buy a calibration diluent, wherein the dilution is 0NU / mL, 2NU / mL, 5NU / mL, 10NU / mL, 30NU / mL and 60NU/ mL respectively. The kit of the invention can be used for detecting O-type foot-and-mouth disease virus antibody, has the advantages of high sensitivity and wide detection range, and realizes the quantitative detection of foot-and-mouth disease antibody.

The invention belongs to the fields of radiopharmaceuticals and nuclear medicine and provides an imaging drug <68>Ga-NOTA-IF7 targeting Anxa1 in tumor blood vessels and a preparation method thereof. The drug <68>Ga-NOTA-IF7 is used for differential diagnosis and periodization of tumors, accurate positioning of focuses and curative effect monitoring. According to the invention, the tumor imaging drug <68>Ga-NOTA-IF7 is prepared through leaching of <68>Ga and <68>Ga-NOTA-IF7 labeling. The preparation method has the advantages of simple preparation technology, convenient operation, small consumption of time, a high labeling rate and a stable marker and facilitates further application of the drug in clinical practice, scientific research and medicinal development. The invention also provides a novel positive electron tumor imaging agent. The novel positive electron tumor imaging agent has good targeting to tumors, so tumor imaging effects are improved, and a visual tool is provided for differential diagnosis and periodization of tumors, accurate positioning of focuses and curative effect monitoring. <68>Ga-NOTA-IF7 is a labeled compound belonging to polypeptide and has the advantages of small molecular weight, low immunogenicity, good tissue penetrability, high tumor tissue affinity and good application prospects.

The invention discloses a Clenbuterol hydrochloride detection test paper and a detection method thereof. The Clenbuterol hydrochloride detection test paper comprises a support and fixing lining which is sequentially provided with a sample adsorption layer, a combination pad, a microporous siphon reaction layer and a water absorption layer; the combination pad is provided with a Clenbuterol hydrochloride antibody labeled by a trace particle and an antibody A labeled by the trace particle, and the antibody A is different from the Clenbuterol hydrochloride antibody; the microporous siphon reaction layer is sequentially provided with a blotting area and a detection area of Clenbuterol hydrochloride carrier protein solution, and the detection area comprises a detection solution blotting area and a control solution blotting area. Two lines occur in the detection area when the content of the Clenbuterol hydrochloride is more than 5 nanogram in a sample; and one line occurs in the detection area when the content of the Clenbuterol hydrochloride is less than 5 nanogram in the sample. The test paper has a detection mode and a reading manner equivalent to those of a sandwich method and low cost. The detection method is simple and rapid, and has simple operation.

The invention discloses novel radioactive 18F labeled amino acid derivatives, which are used in research on tumor positron emission tomography (PET) imaging. The derivatives are characterized in that one end of the derivatives is provided with F substituted alkoxy benzoyl structure, and the other end of the derivatives is provided with alpha-amino acid structure; substituent R1 is positioned on an alpha site of carboxyl group and is hydrogen, methyl group, ethyl group, propyl group, isopropyl group, butyl group, methylthio-ethyl group, methyl acetate group or propionate carbomethoxy group; R2 is methoxy group; and n is a number between 1 and 5. The R1 is hydrogen, methyl group, ethyl group, propyl group, isopropyl group, butyl group, methylthio-ethyl group, methyl acetate group or propionate carbomethoxy group; the R2 is hydrogen; and the n is a number between 1 and 5. Compounds improve fat solubility. Different amino acid structures are introduced into the structure, and F in the structure is 19F and 18F. Compared with the prior art, the 18F labeled amino acid derivatives provided by the invention have better discrimination degree of biological distribution, the potential of being used as a tumor imaging agent (particularly a brain tumor imaging agent), as well as the characteristics of simple preparation and high labeling rate. The R1 is hydrogen, methyl group, ethyl group, propyl group, isopropyl group, butyl group, methylthio-ethyl group, methyl acetate group or propionate carbomethoxy group; the R2 is methoxy group; and the n is a number between 1 and 5. The R1 is hydrogen, methyl group, ethyl group, propyl group, isopropyl group, butyl group, methylthio-ethyl group, methyl acetate group or propionate carbomethoxy group; the R2 is hydrogen; and the n is a number between 1 and 5.

The present invention relates to a test strip which is used for testing a kind of or a plurality of kinds of disease antibodies of porcine virus diarrhea; the test strip comprises a supporting layer and a reaction reagent carrier absorbing layer which is pasted on the supporting layer; the reaction reagent carrier absorbing layer comprises a testing end fiber layer, a fiber layer of absorbing gold-labeled SPA protein or gold-labeled antigen which corresponds to the antigen to be tested, and a cellulose layer, which are arranged at the sample end in sequence, and an absorbent material layer which is positioned at the handle end; the cellulose layer contains one, two or three testing print(s) which are printed by anyone, any two or three of the purified transmissible gastroenteritis virus TGEV solution, the porcine epidemic diarrhea virus PDEV solution and the porcine rotavirus RV, and the cellulose layer also contains the contrast prints which are printed by anyone, any two or three of the anti-SPA protein IgG solution of the sheep or the rabbit or the anti-TGEV, anti-PDEV, anti-RV IgG solution of the sheep or the rabbit; and the invention provides a test strip for testing a kind of or a plurality of kinds of disease antibodies of the porcine virus diarrhea, which has the advantages of accurate and rapid detection, convenient operation and low costs.

The invention designs an 18F labeled p-nitro benzoyl amino acid compound, which is characterized by simultaneously having 2-18F-4-nitro-benzoyl structure and an alpha-amino acid structure, and a substituent R positioned on a carboxyl alpha position, wherein the substituent R comprises hydrogen, methyl, ethyl, propyl, isopropyl, butyl, isobutyl, benzyl, 2-methylthio-ethyl, carboxyethyl, carboxy-propyl, phenyl and imidazole methyl. Two end structures are directly connected with each other through an amido bond. The compound has a structure formula A. The compound can be synthesized simply and has a high labeling rate. The compound is absorbed for a large amount in tumor tissues and is eliminated slowly, but in normal tissues and blood, the compound is absorbed for a small amount or is eliminated quickly. The compound has a very high tumor/blood specific value and a very high tumor/normal tissue specific value, in particular a very high brain normal tissue specific value, and has a high discrimination degree of tumor and brain background. The invention also relates to the application of the compound as a PET brain tumor imaging agent.

The invention relates to a colloid gold chromatographic test paper strip and a preparation method thereof for fast detecting sibutramine, belonging to the field of detection technique of biological immunoassay methods. The test paper strip comprises a scaleboard, a sample pad, a gold-marking binding pad, a coated film and a water absorbent pad, wherein the sample pad, the gold-marking binding pad, the coated film and the water absorbent pad are sequentially spliced on the scaleboard; the gold-marking binding pad is glass cellucotton for absorbing gold-marking antibodies of sibutramine ramifications; the coated film has a linear adelomorphic detection line T printed by carrier protein solution coupled by the sibutramine ramifications and a linear adelomorphic contrasting line C printed by goat anti-rabbit IgG solution, and the two lines are arranged in parallel. The test paper strip has strong specificity, high sensibility, strong timeliness detected minimum reaching 5ppb, visual, directly-viewing and accurate result display and wide scope of population, is simple, convenient and fast, does not need other reagents and instruments, can be operated in field, can determine detection results within 15 minutes when being added to detected sample solution, and saves cost.

The invention designs novel 18F labeled p-nitro benzoyl amino acid derivatives. The derivatives are characterized in that one end of the derivatives is provided with 2-fluorine 18-4-nitro benzoyl structure, and the other end of the derivatives is provided with alpha-amino acid derivative structure (derived group R2 is methoxyl group, methylamino group and N,N-dimethylamino group respectively); and substituent R1 is positioned on an alpha site of carbonyl group and is hydrogen, methyl group, ethyl group, propyl group, isopropyl group, butyl group, isobutyl group, benzyl group, 2-methylthio-ethyl group, carboxyethyl group, carboxypropyl group, phenyl or imidazole methyl group. The structures at two ends are directly connected through amido bond. The structure of the derivatives is shown in a formula A. The compounds are simple to synthesize, fast to label, large in the amount of initial absorption and slow to clear in tumor tissue, low in uptake or fast to clear in other normal tissue and blood and high in degree of distinguishing tumor from background. The invention also relates to the application of the compounds serving as a PET brain tumor imaging agent. R2=OMe, HNCH3.

The invention discloses a clopidol colloidal gold chromatography detection test strip or card which comprises a lining board, a sample pad, a gold mark combined pad, a nitrocellulose membrane and a glass water-absorbing pad, wherein the sample pad, the gold mark combined pad, the nitrocellulose membrane and the glass water-absorbing pad are sequentially connected on the lining board; the gold mark combined pad is an anti-clopidol monoclonal antibody or a polyclonal antibody glass fiber cotton which is absorbed with a colloidal gold mark; and an invisible detection line printed by using a clopidol-carrier protein conjugate and an invisible comparison line printed by using goat anti-mouse IgG are positioned on the nitrocellulose membrane. The clopidol colloidal gold chromatography detection test strip and card disclosed by the invention has the advantages of high specificity, high sensitivity, easiness, convenience, high speed, high timeliness, vivid, visual and accurate result display, cost saving, wide application range and convenience for popularization.

The invention discloses a spectinomycin, streptomycin, gentamycin and neomycin detection test strip and a method thereof. The test strip comprises micro-porous strips, micro-porous reagents, a test paper and micro-porous plugs, the micro-porous reagents are lyophilized colloidal gold labeled spectinomycin, streptomycin, gentamycin and neomycin specific antibodies respectively, the test paper is formed by sequentially connecting a sample absorption pad, a reaction film, a water absorption pad, a protection film and a substrate, the reaction film comprises four detection lines and a quality control line, the four detection lines are coated with a spectinomycin-carrier protein conjugate, a streptomycin-carrier protein conjugate, a gentamycin-carrier protein conjugate and a neomycin-carrier protein conjugate respectively, and the quality control line is coated with an antiantibody. The method for detecting the spectinomycin, streptomycin, gentamycin and neomycin by using the test strip has the advantages of simplicity, rapidness, perceptual intuition, accuracy, portability, wide application range, low cost, and easy popularized use.

The invention discloses novel radioactive 18F labeled aromatic amino acids, which is characterized in that one end of the acids is provided with F substituted alkoxy benzoyl structure, and the other end of the derivatives is provided with alpha-amino acid structure; substituent R1 is positioned on an alpha site of carboxyl group and is phenyl group, benzyl group or 3-indole methyl group; R2 is methoxyl group; and n is a number between 1 and 5. The substituent R1 is positioned on an alpha site of carboxyl group and is phenyl group, benzyl group or 3-indole methyl group; the R2 is methoxyl group; and the n is a number between 1 and 5. Compounds improve fat solubility. Different amino acid structures are introduced into the structure, and F in the structure is 19F and 18F. The substituent R1 is positioned on an alpha site of carboxyl group and is phenyl group, benzyl group or 3-indole methyl group; the R2 is methoxyl group; and the n is a number between 1 and 5. The substituent R1 is positioned on an alpha site of carboxyl group and is phenyl group, benzyl group or 3-indole methyl group; the R2 is methoxyl group; and the n is a number between 1 and 5. Compared with the prior art, the 18F labeled amino acid derivatives provided by the invention have better discrimination degree of biological distribution, the potential of being used as a tumor imaging agent (particularly a brain tumor imaging agent), as well as the characteristics of simple preparation and high labeling rate.

The invention provides a colloidal gold chromatography test strip for quickly detecting lead ions and belongs to the technical field of immunology. The colloidal gold chromatography test strip consists of a lining plate and a sample pad, a gold labeled combination pad, a coating film and an absorbent pad which are connected with the lining plate sequentially, wherein the gold labeled combination pad is glass fiber cotton for absorbing lead ion gold labeled antibodies; a linear hidden detection T line printed by using solution in which the lead ions are coupled with a carrier protein and a linear hidden contrast line C printed by using goat anti mouse IgG are formed on the coating film sequentially; and the two lines are arranged in parallel. The colloidal gold chromatography test strip for quickly detecting the lead ions has high specificity and high sensitivity, can detect lead ion residues more quickly, sensitively and simply, is vivid, intuitive, accurate, simple and clear in result judgment, avoids manmade misjudgment such as false positive, false negative and the like, saves cost, is convenient to popularize and has wide market prospect, outstanding economic and social benefits and a wide application range.

The invention discloses an immunochromatography test strip for detecting capsaicin in gutter oil and a preparation method thereof. The immunochromatography test strip comprises a supporter and an adsorption layer, wherein the adsorption layer is fixedly arranged on the supporter; an adsorption fiber layer, a gold label antibody fiber layer, a cellulose membrane layer and a water-absorbing materiallayer are sequentially arranged on the adsorption layer from the testing end; the cellulose membrane layer is provided with a hidden detection imprint which is printed by a capsaicin-coupled carrierprotein solution, and a hidden contrast imprint which is printed by goat anti-mouse IgG, rabbit anti-mouse IgG or goat anti-rabbit IgG antibody solutions; the gold label antibody fiber layer is made of glass fiber cotton adsorbed with gold label antibody, and the gold label antibody is nanogold particle-labeled capsaicin monoclonal antibody or multiclonal antibody. The immunochromatography test strip has the advantages that the specificity is strong; the sensitivity is high, and 1.7ng/ml of capsaicin can be detected; the operation is simple and convenient, the detection is quick, the detectionresult can be judged within 5min, the result display is visualized, intuitive and accurate, the application range is wide, and the immunochromatography test strip is suitable for being popularized and applied.

The invention relates to a preparation method of a colloidal gold chromatography test paper tape which can test nitrofurantoin metabolite and belongs to the field of testing technology. The test paper tape consists of a lining board and a sample gasket, a gold standard bonding gasket, peridium membrane and an absorbent gasket which are jointed together in sequence on the lining board. The gold standard bonding gasket is glass fiber cotton which can absorb the gold-standard antibody of AHD derivative of nitrofurantoin metabolite. A test line (T) printed by the coupling carrier protein solution of AHD derivative and a control line (C) printed by Goat anti-Rb IgG solution are arranged in parallel with each other on the peridium membrane. The colloidal gold chromatography test paper tape is prepared on the bases that colloidal gold is used for marking a high-affinity polyclonal antibody. The test paper tape has high specificity and sensitivity, and the minimum limit which can be tested can reach 5ppb. The test paper tape can be used easily and conveniently on the spot without any other test reagent and instruments, and the test result can be obtained quickly, thereby achieving high timeliness. After the test paper tape is put into the tested sample solution, the test results can be determined in 15 minutes, and the results can be shown vividly, directly and correctly. The test paper tape can save cost and has wide scope of application; therefore, the test paper tape can be promoted conveniently.

The invention discloses a PD-L1-targeted polypeptide derivative and a 99mTc complex of the PD-L1-targeted polypeptide derivative. The PD-L1-targeted polypeptide derivative and the 99mTc complex respectively have structures shown in structural formulas I and II. The PD-L1-targeted polypeptide derivative is labeled with 99mTc to form the complex, and the complex is used as a PD-L1 specific SPECT/CTdeveloping agent. The developing agent has the advantages that the PD-L1 positive tumor specificity is high, any toxic or side effect is small, and the absorbing dose is low; the developing agent is avery excellent SPECT/CT developing agent. The structure formulas I and II are shown in the attached figures.

The invention belongs to the technical field of fluorescence in situ hybridization, and in particular relates to a ready-to-use multi-probe hybridization glass slide and a preparation method and application thereof. The multi-probe hybridization slide is composed of a probe attachment slide, each probe, a hybridization buffer and a sample attachment slide; each probe is respectively attached to each independent probe attachment area in a freeze-dried form; The sample is dropped on the sample attachment area, and when the sample attachment area and the probe attachment area are attached, the sample in the sample attachment area is hybridized with the probe in the corresponding probe attachment area. Compared with the traditional FISH experiment, the present invention can simultaneously preset multiple groups of probes on one glass slide, and can complete the detection of various probes on the same slide, greatly simplifying the operation steps of traditional FISH; The usage amount of the probe reduces the usage cost of the probe; the ready-to-use multi-probe hybridization glass slide of the present invention has extremely high signal-to-noise ratio, specificity and sample detection rate.

The invention discloses a quantum dot test strip for rapidly detecting trace fipronil and a preparation method of the quantum dot test strip. According to the test strip, a bottom layer is a supporting layer, a middle layer is an adsorption layer, a protecting layer is fixed on the adsorption layer, the adsorption layer comprises an adsorption fiber layer, a quantum dot labeled antibody fiber layer, a cellulose membrane layer and a water absorbing material layer at a handle end sequentially from the test end, wherein a cellulose membrane is coated with fipronil coupled carrier protein to serveas a detection line, and a quality control line printed by goat anti or rabbit anti-mouse IgG, goat anti rabbit IgG antibody or SPA is also arranged. A test indicates that the test strip has higher specificity and higher sensitivity, limit of visual detection is 1 ng/mL, and the test strip is free of cross reaction with other pesticides or veterinary drugs and has quite great significance in theaspects of guaranteeing food safety and protecting health of consumers.

The invention discloses pseudorabies antibody blocking test paper, composed of a supporting plate, an antigen pad, a gold labeling pad, detection film and a water absorption pad. The antigen pad absorbs pseudorabies virus detection antigens. The gold labeling pad absorbs colloidal gold labeled anti-pseudorabies virus gE and gB monoclonal antibodies mAb1 and mAb2. Virulent detection line T1''|'', vaccine virus detection line T2''|'' and quality control line C''|'' prints are set on the detection film. The virulent detection line T1 is an anti-pseudorabies virus gE protein monoclonal antibody mAb3 print. The vaccine virus detection line T2 is an anti-pseudorabies virus gB protein monoclonal antibody mAb4 print. The quality control line C is a rabbit anti-mouse IgG antibody pAb1 or staphylococcus aureus SPA print ''|''. According to the test paper, rapid detection and real-time detection of pseudorabies virus infection and immune antibodies are realized, infection and immune detection andimmune evaluation of a vaccine immune effect are realized, operation is simple, and the test paper is easy to popularize and apply in a wide range.

The invention belongs to the technical field of biological medicines, and particularly relates to a PSMA inhibitor, an application thereof and a PSMA-targeting nuclide imaging reagent. The PSMA inhibitor has a structure as shown in a formula I. The PSMA-targeting nuclide imaging reagent prepared by adopting EDDA as a co-ligand has good PSMA targeting property and affinity; high stability is realized in normal saline and mouse serum; meanwhile, the cell uptake amount is relatively high, and the metabolic performance is good. Therefore, the inhibitor has a good clinical application prospect in tumor imaging of targeted PSMA.