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Colloid gold chromatographic test paper strip and preparation method thereof for fast detecting sibutramine

A technology for detecting test strips and sibutramine, which is applied in the detection field of biological immune methods, can solve the problems of application limitation of ELISA method, complicated operation process and the like, and achieves the effects of easy promotion, high sensitivity and cost saving.

Inactive Publication Date: 2009-09-09
JIANGNAN UNIV
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  • Abstract
  • Description
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Problems solved by technology

However, the ELISA method requires a matching microplate reader and supporting reagents, the operation process is still relatively complicated, and it is currently in the research and development stage at home and abroad, so the application of the ELISA method is greatly restricted

Method used

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  • Colloid gold chromatographic test paper strip and preparation method thereof for fast detecting sibutramine
  • Colloid gold chromatographic test paper strip and preparation method thereof for fast detecting sibutramine

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preparation example Construction

[0038] 2. Preparation of polyclonal antibody against sibutramine derivatives

[0039] Polyclonal antibody preparation: use M 2 - New Zealand white rabbits were immunized with BSA antigen, the immunization dose was 1mg / time, and multi-point subcutaneous injection was given on the back. For the first time, use M 2 -BSA antigen mixed with an equal amount of Freund's complete adjuvant (FCA), fully emulsified; 2 -BSA antigen mixed with an equal amount of Freund's incomplete adjuvant (FIA), fully emulsified, 3 weeks after the first immunization, 4-5 consecutive immunizations, each interval of 2-3 weeks, 10-15 days after the last immunization , measured by ELISA method and its fixed titer reached 10 5 At the above time, blood was collected and serum was separated and collected. IgG antibodies were extracted by saturated ammonium sulfate salting-out method, and frozen at -20°C for later use.

[0040] 3. Preparation of sibutramine derivative gold-labeled antibody and gold-labeled ...

Embodiment 1

[0044] Example 1: Spray the selected concentration of coating antigen and goat anti-rabbit IgG on the coating film as the test line (T) and control line (C) respectively, and dry in an oven at 37°C for 10 minutes. In the same way, a certain concentration of gold-labeled antibody was coated on the binding pad. The test strip is composed of a backing plate, on which a sample pad, a colloidal gold bonding pad, a coating film and a water-absorbing pad are glued in sequence. Cut the affixed board into 3mm wide strips, and then put the test strips together with the desiccant into an aluminum foil bag and seal them for storage.

[0045] Detection operation method: insert the sample end of the sibutramine colloidal gold chromatography test strip into the sample solution to be tested, the insertion depth does not exceed the marking line, take out the test strip for about 10-20 seconds, place it horizontally, and wait for 15 minutes Observe left and right to judge the test result.

[...

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Abstract

The invention relates to a colloid gold chromatographic test paper strip and a preparation method thereof for fast detecting sibutramine, belonging to the field of detection technique of biological immunoassay methods. The test paper strip comprises a scaleboard, a sample pad, a gold-marking binding pad, a coated film and a water absorbent pad, wherein the sample pad, the gold-marking binding pad, the coated film and the water absorbent pad are sequentially spliced on the scaleboard; the gold-marking binding pad is glass cellucotton for absorbing gold-marking antibodies of sibutramine ramifications; the coated film has a linear adelomorphic detection line T printed by carrier protein solution coupled by the sibutramine ramifications and a linear adelomorphic contrasting line C printed by goat anti-rabbit IgG solution, and the two lines are arranged in parallel. The test paper strip has strong specificity, high sensibility, strong timeliness detected minimum reaching 5ppb, visual, directly-viewing and accurate result display and wide scope of population, is simple, convenient and fast, does not need other reagents and instruments, can be operated in field, can determine detection results within 15 minutes when being added to detected sample solution, and saves cost.

Description

technical field [0001] The invention discloses a colloidal gold chromatography test strip for rapid detection of sibutramine and a preparation method thereof, which belong to the detection technical field of biological immune methods. Background technique [0002] Sibutramine hydrochloride, English name: Sibutramine Hydrochloride, its chemical name is: (±)N-{1-[1-(4-chlorophenyl)cyclobutyl]-3-methylbutyl}-N, N-dimethylamine hydrochloride monohydrate, CAS No. 106650-56-0, molecular formula is C 17 h 26 ClN·HCl·H 2 O, molecular weight 334, is the hydrochloride hydrate of sibutramine. The drug was developed by German Kno ll company and was approved by the US FDA in November 1997 to be marketed in the United States; in May 2000, the drug was approved by the State Drug Administration (SDA) to be marketed in China for the treatment of obesity. Sibutramine hydrochloride is an oral centrally acting weight-loss drug. It is rapidly absorbed after oral administration, and is metabo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/552
Inventor 胥传来李灼坤刘丽强马伟
Owner JIANGNAN UNIV
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