87results about How to "The result display is intuitive" patented technology

The invention discloses an enzyme linked immunosorbent assay kit used for detecting the content of heavy metal copper ions in a sample. The kit includes a coat antigen coated enzyme label plate, an enzyme marker, a copper ion chelate specific monoclonal antibody or polyclonal antibody, a copper ion chelate standard solution, a substrate color development solution, a terminating solution, a lotion, and a diluted chelating agent EDTA. The enzyme linked immunosorbent assay kit used for copper ion detection has the advantages of: (1) strong specificity, high sensitivity, a lowest detection limit of 0.42 microgram/L, and a detection range of 2.83-456.04 microgram/L; (2) simplicity, rapidity, and strong timeliness; (3) vivid, visual and accurate result display; and (4) low cost, wide application range, convenient popularization, on-site supervision and suitability for screening a large number of samples.

The invention relates to a test paper strip for rapidly detecting traces of chlorothalonil and a preparation method thereof. The test paper strip is characterized in that a supporting layer is used as the bottom layer, an adsorption layer is used as the intermediate layer, a protective layer is fixed on the adsorption layer, the adsorption layer comprises an adsorption fibrous layer, a gold-labeled antibody fibrous layer, a cellulose film and an absorbent material layer at the handle end in order from a test terminal, wherein the cellulose film is provided with detection blots printed by using a chlorothalonil coupling carrier protein solution and control blots printed by using a goat anti-mouse IgG or rabbit anti-mouse IgG (or goat anti-rabbit IgG) antibody solution; the gold-labeled antibody is a colloidal gold labeled chlorothalonil monoclonal antibody or polyclonal antibody; and the chlorothalonil coupling carrier protein is bovine serum albumin, chicken ovalbumin or haemocyanin. The test paper strip disclosed herein has the advantages of strong specificity, high sensitivity, simple, and accurate detection, low cost, wide application scope, and easiness in popularization and application.

The invention relates to a colloid gold chromatographic test paper strip and a preparation method thereof for fast detecting sibutramine, belonging to the field of detection technique of biological immunoassay methods. The test paper strip comprises a scaleboard, a sample pad, a gold-marking binding pad, a coated film and a water absorbent pad, wherein the sample pad, the gold-marking binding pad, the coated film and the water absorbent pad are sequentially spliced on the scaleboard; the gold-marking binding pad is glass cellucotton for absorbing gold-marking antibodies of sibutramine ramifications; the coated film has a linear adelomorphic detection line T printed by carrier protein solution coupled by the sibutramine ramifications and a linear adelomorphic contrasting line C printed by goat anti-rabbit IgG solution, and the two lines are arranged in parallel. The test paper strip has strong specificity, high sensibility, strong timeliness detected minimum reaching 5ppb, visual, directly-viewing and accurate result display and wide scope of population, is simple, convenient and fast, does not need other reagents and instruments, can be operated in field, can determine detection results within 15 minutes when being added to detected sample solution, and saves cost.

The invention discloses a fluorescence immunoassay chromatography test strip for detecting vomitoxin. The fluorescence immunoassay chromatography test strip comprises a supporting body and an adsorption layer fixed to the supporting body. The adsorption layer sequentially comprises an adsorption fiber layer, a fluorescent antibody fiber layer, a cellulose membrane layer and a water absorption material layer starting from the test end. Invisible detection prints printed by a coupling DON carrier protein solution and invisible contrast prints printed by a goat anti-mouse IgG or rabbit anti-mouse IgG or goat anti-rabbit IgG antibody solution are arranged on the cellulose membrane layer. The fluorescent antibody fiber layer is made from glass fiber cotton for adsorbing fluorescent antibodies. The fluorescent antibodies are graphene oxide fluorescent nanometer materials or NaYF4:Yb or Tm nano-particles or DON monoclonal antibodies or polyclonal antibodies marked by NaGd(WO4)2:Eu3+ nano-particles. The test strip has the advantages of being high in specificity, sensitivity and stability, good in safety and easy, convenient and rapid to use, can achieve on-site quantitative detection under a portable fluorescent reading device and can meet the requirements of people of different levels.

The invention discloses a clopidol colloidal gold chromatography detection test strip or card which comprises a lining board, a sample pad, a gold mark combined pad, a nitrocellulose membrane and a glass water-absorbing pad, wherein the sample pad, the gold mark combined pad, the nitrocellulose membrane and the glass water-absorbing pad are sequentially connected on the lining board; the gold mark combined pad is an anti-clopidol monoclonal antibody or a polyclonal antibody glass fiber cotton which is absorbed with a colloidal gold mark; and an invisible detection line printed by using a clopidol-carrier protein conjugate and an invisible comparison line printed by using goat anti-mouse IgG are positioned on the nitrocellulose membrane. The clopidol colloidal gold chromatography detection test strip and card disclosed by the invention has the advantages of high specificity, high sensitivity, easiness, convenience, high speed, high timeliness, vivid, visual and accurate result display, cost saving, wide application range and convenience for popularization.

The invention provides a colloidal gold chromatography test strip for quickly detecting lead ions and belongs to the technical field of immunology. The colloidal gold chromatography test strip consists of a lining plate and a sample pad, a gold labeled combination pad, a coating film and an absorbent pad which are connected with the lining plate sequentially, wherein the gold labeled combination pad is glass fiber cotton for absorbing lead ion gold labeled antibodies; a linear hidden detection T line printed by using solution in which the lead ions are coupled with a carrier protein and a linear hidden contrast line C printed by using goat anti mouse IgG are formed on the coating film sequentially; and the two lines are arranged in parallel. The colloidal gold chromatography test strip for quickly detecting the lead ions has high specificity and high sensitivity, can detect lead ion residues more quickly, sensitively and simply, is vivid, intuitive, accurate, simple and clear in result judgment, avoids manmade misjudgment such as false positive, false negative and the like, saves cost, is convenient to popularize and has wide market prospect, outstanding economic and social benefits and a wide application range.

The invention discloses an immunochromatography test strip for detecting capsaicin in gutter oil and a preparation method thereof. The immunochromatography test strip comprises a supporter and an adsorption layer, wherein the adsorption layer is fixedly arranged on the supporter; an adsorption fiber layer, a gold label antibody fiber layer, a cellulose membrane layer and a water-absorbing materiallayer are sequentially arranged on the adsorption layer from the testing end; the cellulose membrane layer is provided with a hidden detection imprint which is printed by a capsaicin-coupled carrierprotein solution, and a hidden contrast imprint which is printed by goat anti-mouse IgG, rabbit anti-mouse IgG or goat anti-rabbit IgG antibody solutions; the gold label antibody fiber layer is made of glass fiber cotton adsorbed with gold label antibody, and the gold label antibody is nanogold particle-labeled capsaicin monoclonal antibody or multiclonal antibody. The immunochromatography test strip has the advantages that the specificity is strong; the sensitivity is high, and 1.7ng/ml of capsaicin can be detected; the operation is simple and convenient, the detection is quick, the detectionresult can be judged within 5min, the result display is visualized, intuitive and accurate, the application range is wide, and the immunochromatography test strip is suitable for being popularized and applied.

The invention relates to a preparation method of a colloidal gold chromatography test paper tape which can test nitrofurantoin metabolite and belongs to the field of testing technology. The test paper tape consists of a lining board and a sample gasket, a gold standard bonding gasket, peridium membrane and an absorbent gasket which are jointed together in sequence on the lining board. The gold standard bonding gasket is glass fiber cotton which can absorb the gold-standard antibody of AHD derivative of nitrofurantoin metabolite. A test line (T) printed by the coupling carrier protein solution of AHD derivative and a control line (C) printed by Goat anti-Rb IgG solution are arranged in parallel with each other on the peridium membrane. The colloidal gold chromatography test paper tape is prepared on the bases that colloidal gold is used for marking a high-affinity polyclonal antibody. The test paper tape has high specificity and sensitivity, and the minimum limit which can be tested can reach 5ppb. The test paper tape can be used easily and conveniently on the spot without any other test reagent and instruments, and the test result can be obtained quickly, thereby achieving high timeliness. After the test paper tape is put into the tested sample solution, the test results can be determined in 15 minutes, and the results can be shown vividly, directly and correctly. The test paper tape can save cost and has wide scope of application; therefore, the test paper tape can be promoted conveniently.

The invention discloses a quantum dot test strip for rapidly detecting trace fipronil and a preparation method of the quantum dot test strip. According to the test strip, a bottom layer is a supporting layer, a middle layer is an adsorption layer, a protecting layer is fixed on the adsorption layer, the adsorption layer comprises an adsorption fiber layer, a quantum dot labeled antibody fiber layer, a cellulose membrane layer and a water absorbing material layer at a handle end sequentially from the test end, wherein a cellulose membrane is coated with fipronil coupled carrier protein to serveas a detection line, and a quality control line printed by goat anti or rabbit anti-mouse IgG, goat anti rabbit IgG antibody or SPA is also arranged. A test indicates that the test strip has higher specificity and higher sensitivity, limit of visual detection is 1 ng/mL, and the test strip is free of cross reaction with other pesticides or veterinary drugs and has quite great significance in theaspects of guaranteeing food safety and protecting health of consumers.

The invention discloses a colloidal gold chromatography test strip for quickly detecting sibutramine and derivative thereof and a preparation method thereof. The test strip consists of a lining plate and a sample pad, a gold label bonding pad, a coating film and a water absorption pad engaged on the lining plate in turn, wherein on the coating film, a straight detection line T is coated by using carrier protein solution coupled with the sibutramine and the derivative thereof, and a straight contrast line C is coated by using goat-anti-mouse IgG solution. The method comprises the following steps of: preparing artificial conjugated antigens; preparing monoclonal antibodies of the sibutramine and the derivative thereof; preparing colloidal gold solution; and obtaining a colloidal gold marker and the gold label bonding pad for resisting the monoclonal antibodies of the sibutramine and the derivative thereof. The test strip has the advantages of strong specificity, high sensitivity, strong timeliness and vivid and accurate result display, and is simple, convenient and quick.

The present invention discloses a phenobarbital quick detection test paper strip. It contains bottom layer as supporting layer, intermediate layer as adsorption layer and protective layer fixed on the adsorption layer, Said adsorption layer successively includes test end adsorption fibre layer, adsorption gold-labelled amtibody fibre layer, cellulose membrane layer and water-absorbing material layer of handle end. Besides, said invention also provides the concrete structure on the cellulose membrane layer, said structure include linear detection blotting and linear reference blotting.

The invention discloses a fluorescence immunochromatography test paper for detecting zearalenone. The test paper comprises a supporting body and an adsorption layer fixed on the supporting body, the adsorption layer sequentially comprises an adsorption fiber layer, a fluorescence antibody fiber layer, a cellulose film and a water absorbing material layer, and the cellulose film is provided with a ZEN-coupled carrier protein solution printed shealth detection blot and a goat anti-mouse IgGT, rabbit anti-mouse IgG or goat anti-rabbit IgG antibody solution printed shealth contrast blot; and the fluorescence antibody fiber layer is made of fluorescence antibody adsorbing glass fiber cotton, and the fluorescence antibody is a graphene oxide fluorescence nano-material, and a NaYF4:Yb, Tm nanoparticle or NaGd(WO4)2:Eu<3+> nano-particle labeled ZEN monoclonal antibody or polyclonal antibody. The test paper has the characteristics of strong specificity, high sensitivity, high stability, good safety, high simplicity, fastness, realization of onsite quantitative detection under a portable fluorescence reader, and meeting of needs of people with different levels.

The invention relates to an anti-isofenphos-methyl monoclonal antibody as well as a preparation method and an application thereof, an amino acid sequence of an antibody heavy chain variable region ofthe anti-isofenphos-methyl monoclonal antibody is as shown in SEQ ID NO: 1, and the amino acid sequence of a light chain variable region of the anti-isofenphos-methyl monoclonal antibody is as shown in SEQ ID NO: 2; compared with the prior art, the monoclonal antibody has the advantages that: 1) the antibody has outstanding performance; 2) a test strip is specific, sensitive, simple, convenient and rapid, and 3) the result is vivid, intuitive and accurate: the detection result of the colloidal gold test strip is judged according to the color development condition on a cellulose membrane, the result display is vivid, visual, accurate, simple and clear; and 4) the cost is saved, the application range is wide, the popularization is convenient, and the monoclonal antibody has wide market prospect and obvious economic and social benefits.

The invention relates to a test strip for rapidly detecting trace erythrosine and a preparation method. According to the test strip, a bottom layer is a supporting layer, a middle layer is an adsorption layer and a protection layer is fixed on the adsorption layer, wherein the adsorption layer is composed of an adsorption fiber layer, a gold-labeled antibody layer, a cellulose membrane layer and a water absorption material layer in sequence from a testing end; the cellulose membrane layer is provided with a detection print printed by an erythrosine coupled carrier protein solution and a contrast print printed by a goat or rabbit anti-mouse IgG (Immunoglobulin G) solution or a goat anti-rabbit IgG solution; an erythrosine gold-labeled antibody is a colloidal gold labeled erythrosine monoclonal antibody or polyclonal antibody; the erythrosine coupled carrier protein solution is a coupled compound solution of erythrosine and a carrier protein. The test strip provided by the invention is strong in specificity and high in sensitivity and is simple, visualized and accurate; an applicable range is wide and the cost is low; therefore, the test strip is easy to popularize and apply.

The invention relates to a drainage pumping station water pump operation performance determining method and equipment. The determining method comprises the following steps: S1: loading rated drainage volumes of water pumps within a set time period; S2: collecting the actual drainage volumes of the water pumps within the set time period; S3: determining the operation performance of the water pumps and generating related coefficients according to the actual drainage volumes and the rated drainage volumes; S4: displaying the determination results to maintenance staff in a visible manner. Compared with the prior art, the method provided by the invention has the advantages as follows: the operation performance of the water pumps is determined by calculating the related coefficients according to the actual drainage volumes of the water pumps, collected within the set time period, and the rated drainage volumes, so that the determination results can reflect the operation states of the water pumps more objectively; besides, the determination results are displayed to the maintenance staff in a visible manner, so that the displayed results are more visual, and the experience of maintenance staff is better.

The invention discloses a test strip for quickly detecting oxolinic acid and a preparation method thereof. A bottom layer of the test strip is a support layer; a middle layer is an adsorbed layer; a protecting layer is fixed on the adsorbed layer; the adsorbed layer is orderly divided into an adsorption fiber layer, a gold-labelled antibody fiber layer, a cellulose membrane layer and an absorbent material layer at the handle end from a test end; the gold-labelled antibody in the gold-labelled antibody fiber layer is an anti-oxolinic acid polyclonal antibody or monoclonal antibody labelled by colloidal gold; a detection imprinting and a contrast imprinting are arranged on the cellulose membrane layer; the detection imprinting is printed by carrier protein coupled to the oxolinic acid; the contrast imprinting is printed by a goat anti-rabbit or rabbit anti-mouse IgG antibody or a goat anti-rabbit IgG antibody. The test strip disclosed by the invention is simple in structure, strong in specificity, high in sensitivity and accuracy, simple and convenient to operate, wide in application range, low in price, and easy to popularize and apply, and has better social and economic value.

The invention relates to a chlopyrifos monoclonal antibody and a preparation method and application thereof. The chlopyrifos monoclonal antibody and preparation method and application thereof have theadvantages that the specificity and sensitivity are high, the method is simple and rapid, and a result is displayed vividly, visually and accurately; the cost is reduced, the application range is wide, convenience is provided for popularization; and via the prepared chlopyrifos colloidal gold test paper, the result can be determined within minutes according to strips presented after reaction needless of professionals or auxiliary instruments, operation is simple and rapid, the result is visual and accurate, the cost is low, and detection can be carried out in the outside.

The invention discloses a method for estimating the covering ratio, the transmitting optical power and the mismatch magnitude in a space chaotic laser communication system. At present, setting of keyparameters such as a covering ratio, transmitting light power and a mismatch magnitude in a space chaotic laser communication system is mainly estimated through experiments, but due to mutual influence among the parameters, a method for estimating the parameters through the experiments requires a large amount of repeated operation so that a large amount of time and resources are consumed. The invention provides a simulation estimation method in order to efficiently estimate key parameters such as the covering ratio, the transmitting optical power and the mismatch magnitude in the space chaoticlaser communication system. The method is simple, quick and low in cost, repeated experimental operation is not needed, and the relation between the parameters can be visually seen.

The invention discloses fluorescent immunochromatography test paper for detecting aflatoxin B1. The fluorescent immunochromatography test paper comprises a supporting body and an adsorption layer fixed to the supporting body, and the adsorption layer sequentially comprises an adsorption fiber layer, a fluorescent antibody fiber layer, a cellulose membrane layer and a water absorption material layer starting from the testing end; the cellulose membrane layer is provided with invisible detection imprints imprinted by an AFB1-coupled carrier protein solution and invisible control imprints imprinted by a goat anti-mouse IgG antibody solution or a rabbit anti-mouse IgG antibody solution or a goat anti-rabbit IgG antibody solution; the fluorescent antibody fiber layer is made of glass fiber cotton adsorbing fluorescent antibodies, wherein the fluorescent antibodies are AFB1 monoclonal antibodies or AFB1 polyclonal antibodies which are labeled with graphene oxide fluorescent nanometer materials or NaYF4:Yb, Tm nanoparticles or NaGd(WO4)2:Eu<3+> nanoparticles. The test paper strip has the advantages of being high in specificity, sensitivity and stability, good in safety and easy, convenient and rapid to manufacture, and can achieve on-site quantitative detection in the presence of a portable fluorescent reading meter and meet the requirements of personnel at different levels.