Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Triazophos monoclonal antibody and application thereof to colloidal gold detection test paper

A technology of cloning antibody and triazophos monoclonal antibody, which is applied in the field of application of triazophos monoclonal antibody in colloidal gold detection test paper, can solve the problems of unintuitive detection results, many operation steps, long detection time, etc., and achieve easy promotion, The effect of high sensitivity and result judgment image

Active Publication Date: 2020-01-24
SUZHOU KUAIJIEKANG BIOTECH CO LTD
View PDF10 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

ELISA (Enzyme Linked Immunosorbent Assay) method is a commonly used immunoassay method, but it has relatively many operating steps, a long detection time, the detection result is not intuitive enough, and it cannot be detected online.
Therefore, the application of ELISA in the rapid detection of triazophos is greatly limited.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Triazophos monoclonal antibody and application thereof to colloidal gold detection test paper
  • Triazophos monoclonal antibody and application thereof to colloidal gold detection test paper
  • Triazophos monoclonal antibody and application thereof to colloidal gold detection test paper

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0030] Embodiment: 1, the synthesis of triazophos hapten

[0031] Synthesis of the immune hapten O-ethyl-O-(1-phenyl-1,2,4-triazol-3-yl)-N-(butyryl)phosphorothioate. Weigh 3.3 g of 1-phenyl-3-hydroxy-1,2,4-triazole and dissolve it in acetonitrile; K 2 CO 3 8.7g of the mixed solution, after dripping, continue to react for 1 hour, vacuum filter after the reaction, evaporate the solvent to obtain a yellow oil, column chromatography, wash with petroleum ether / dichloromethane, evaporate the solvent to obtain a light yellow oily liquid . Weigh 0.54g of 4-aminobutyric acid and 0.78g of KOH, dissolve them in 6mL of methanol, cool in an ice-water bath to 0-10°C, slowly add 1.18g of light yellow oily liquid dissolved in 6mL of methanol solution under stirring, and keep warm for 2 hours , after the completion of the reaction, wash with petroleum ether / ether, adjust the pH of the water layer to 3.0, extract with ether, dry over anhydrous sodium sulfate, and evaporate the solvent to ob...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Sensitivityaaaaaaaaaa
Login to View More

Abstract

The invention relates to a triazophos monoclonal antibody. The triazophos monoclonal antibody is characterized in that the amino acid sequence of a heavy-chain variable region of the antibody is shownas SEQID NO:1, and the amino acid sequence of a light-chain variable region of the antibody is shown as SEQID NO:2. The invention relates to an application of the triazophos monoclonal antibody to colloidal gold detection test paper. The colloidal gold detection test paper comprises a liner, as well as a sample pad, a gold marker combination pad, a cellulose membrane and a water absorption pad which are sequentially arrayed on the liner, wherein the inner part of the gold marker combination pad is adsorbed with a triazophos gold marker antibody, a detection line and a control line are formedin the cellulose membrane, the detection line is printed with a triazophos hapten coupled carrier protein solution, carrier protein adopted in the carrier protein solution is prepared through couplingO-ethyl-O-(1-phenyl-1,2,4-triazole-3-based)-N-(butyric acid based) thiophosphate ester with bovine serum albumin (BSA) in an active ester method, and the control line is printed through a rabbit antimouse IgG antibody. The triazophos monoclonal antibody disclosed by the invention is high in specificity, high in sensibility, simple, convenient and quick to detect, vivid, audio-visual and accuratein result display, wide in application range and convenient to popularize, and fees are saved.

Description

technical field [0001] The invention relates to the field of pesticide detection, in particular to the application of a triazophos monoclonal antibody in colloidal gold detection test paper. Background technique [0002] Triazophos belongs to organophosphorus insecticides and is mainly used to control Lepidoptera pests, pest mites, fly larvae and underground pests on fruit trees, cotton, and grain crops. Its mechanism of action is to inhibit the activity of acetylcholinesterase. Acetylcholine is a neurotransmitter, and excess acetylcholine overstimulates acetylcholine receptors, leading to the death of target organisms. However, the residue of triazophos can also poison some organisms that were not the intended target. [0003] At present, the detection methods for triazophos residues are mainly instrumental analysis methods, including gas chromatography (Gaschromatography, GC), gas chromatography-mass spectrometry (GC-MS) and so on. These methods have reliable results an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/44G01N33/577G01N33/558
CPCC07K16/44G01N33/558G01N33/577
Inventor 盛相国
Owner SUZHOU KUAIJIEKANG BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com