Triazophos monoclonal antibody and application thereof to colloidal gold detection test paper

Abstract

The invention relates to a triazophos monoclonal antibody. The triazophos monoclonal antibody is characterized in that the amino acid sequence of a heavy-chain variable region of the antibody is shownas SEQID NO:1, and the amino acid sequence of a light-chain variable region of the antibody is shown as SEQID NO:2. The invention relates to an application of the triazophos monoclonal antibody to colloidal gold detection test paper. The colloidal gold detection test paper comprises a liner, as well as a sample pad, a gold marker combination pad, a cellulose membrane and a water absorption pad which are sequentially arrayed on the liner, wherein the inner part of the gold marker combination pad is adsorbed with a triazophos gold marker antibody, a detection line and a control line are formedin the cellulose membrane, the detection line is printed with a triazophos hapten coupled carrier protein solution, carrier protein adopted in the carrier protein solution is prepared through couplingO-ethyl-O-(1-phenyl-1,2,4-triazole-3-based)-N-(butyric acid based) thiophosphate ester with bovine serum albumin (BSA) in an active ester method, and the control line is printed through a rabbit antimouse IgG antibody. The triazophos monoclonal antibody disclosed by the invention is high in specificity, high in sensibility, simple, convenient and quick to detect, vivid, audio-visual and accuratein result display, wide in application range and convenient to popularize, and fees are saved.

Description

technical field [0001] The invention relates to the field of pesticide detection, in particular to the application of a triazophos monoclonal antibody in colloidal gold detection test paper. Background technique [0002] Triazophos belongs to organophosphorus insecticides and is mainly used to control Lepidoptera pests, pest mites, fly larvae and underground pests on fruit trees, cotton, and grain crops. Its mechanism of action is to inhibit the activity of acetylcholinesterase. Acetylcholine is a neurotransmitter, and excess acetylcholine overstimulates acetylcholine receptors, leading to the death of target organisms. However, the residue of triazophos can also poison some organisms that were not the intended target. [0003] At present, the detection methods for triazophos residues are mainly instrumental analysis methods, including gas chromatography (Gaschromatography, GC), gas chromatography-mass spectrometry (GC-MS) and so on. These methods have reliable results an...

AI Technical Summary

Problems solved by technology

ELISA (Enzyme Linked Immunosorbent Assay) method is a commonly used immunoassay method, but it has relatively many operating steps, a long detection time, the detection result is not intuitive enough, and it cannot be detected online.

Therefore, the application of ELISA in the rapid detection of triazophos is greatly limited.

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