Colloidal gold chromatography test strip for quickly detecting lead ions
A technology for detecting test strips and lead ions, which is applied in the field of immunology to achieve the effects of cost saving, wide application range and broad market prospects
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[0022] 2. Preparation of anti-lead ion monoclonal antibody
[0023] Preparation of monoclonal antibody: Immunize 6-8 week-old female BALB / c mice with lead ionophore protein-bound immunization antigen (Pb-ITCBE-KLH), the immunization dose is 100 μg / time, using rapid adjuvant, and injected into leg muscles. For the first immunization, use the lead ionophore protein immunization antigen and an equal volume of rapid adjuvant to mix 1:1, and mix thoroughly; 3 weeks after the first immunization, booster immunization is carried out, the method is the same as above, and 3-4 consecutive immunizations are performed, with an interval of 3 weeks between each time. 10-15 days after the last immunization. The complete antigen metal ion-chelating agent-carrier protein and the chelating agent-carrier protein were coated on the microtiter plate at the same time, and the mouse serum titer was detected by indirect ELISA. The results showed that the mouse serum titer could reach 1:8000~1:128000, ...
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[0033] Example 1. Spray the selected concentration of coating antigen and goat anti-mouse IgG on the coating film as the test line (T) and control line (C) respectively, and dry in an oven at 37°C for 10 min. In the same way, a certain concentration of gold-labeled antibody was coated on the binding pad. The test strip is composed of a backing plate, on which a sample pad, a gold standard binding pad, a coating film and an absorbent pad are glued in sequence. Cut the affixed board into 3mm wide strips, and then put the test strips together with the desiccant into an aluminum foil bag and seal them for storage.
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