Colloidal gold chromatography test strip for quickly detecting lead ions

A technology for detecting test strips and lead ions, which is applied in the field of immunology to achieve the effects of cost saving, wide application range and broad market prospects

Inactive Publication Date: 2011-11-23
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the application of immunological detection methods to detect heavy metal ions in the environment is still in the experimental stage of the laboratory

Method used

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  • Colloidal gold chromatography test strip for quickly detecting lead ions
  • Colloidal gold chromatography test strip for quickly detecting lead ions
  • Colloidal gold chromatography test strip for quickly detecting lead ions

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0022] 2. Preparation of anti-lead ion monoclonal antibody

[0023] Preparation of monoclonal antibody: Immunize 6-8 week-old female BALB / c mice with lead ionophore protein-bound immunization antigen (Pb-ITCBE-KLH), the immunization dose is 100 μg / time, using rapid adjuvant, and injected into leg muscles. For the first immunization, use the lead ionophore protein immunization antigen and an equal volume of rapid adjuvant to mix 1:1, and mix thoroughly; 3 weeks after the first immunization, booster immunization is carried out, the method is the same as above, and 3-4 consecutive immunizations are performed, with an interval of 3 weeks between each time. 10-15 days after the last immunization. The complete antigen metal ion-chelating agent-carrier protein and the chelating agent-carrier protein were coated on the microtiter plate at the same time, and the mouse serum titer was detected by indirect ELISA. The results showed that the mouse serum titer could reach 1:8000~1:128000, ...

Embodiment 1

[0033] Example 1. Spray the selected concentration of coating antigen and goat anti-mouse IgG on the coating film as the test line (T) and control line (C) respectively, and dry in an oven at 37°C for 10 min. In the same way, a certain concentration of gold-labeled antibody was coated on the binding pad. The test strip is composed of a backing plate, on which a sample pad, a gold standard binding pad, a coating film and an absorbent pad are glued in sequence. Cut the affixed board into 3mm wide strips, and then put the test strips together with the desiccant into an aluminum foil bag and seal them for storage.

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PUM

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Abstract

The invention provides a colloidal gold chromatography test strip for quickly detecting lead ions and belongs to the technical field of immunology. The colloidal gold chromatography test strip consists of a lining plate and a sample pad, a gold labeled combination pad, a coating film and an absorbent pad which are connected with the lining plate sequentially, wherein the gold labeled combination pad is glass fiber cotton for absorbing lead ion gold labeled antibodies; a linear hidden detection T line printed by using solution in which the lead ions are coupled with a carrier protein and a linear hidden contrast line C printed by using goat anti mouse IgG are formed on the coating film sequentially; and the two lines are arranged in parallel. The colloidal gold chromatography test strip for quickly detecting the lead ions has high specificity and high sensitivity, can detect lead ion residues more quickly, sensitively and simply, is vivid, intuitive, accurate, simple and clear in result judgment, avoids manmade misjudgment such as false positive, false negative and the like, saves cost, is convenient to popularize and has wide market prospect, outstanding economic and social benefits and a wide application range.

Description

technical field [0001] The invention relates to a colloidal gold chromatography test strip for rapidly detecting lead ions, a preparation method and application thereof, and belongs to the technical field of immunology. Background technique [0002] Lead is a major raw material in current industrial production, and it is very harmful to the human body. Lead poisoning can cause neurasthenia and indigestion, and lead encephalopathy can occur in severe cases, resulting in epilepsy, dementia or mental irritability, vision loss or even blindness. In 1923, lead was added to gasoline as an antiknock agent, which accelerated global lead pollution. Lead pollution has spread across the globe, and the amount of lead in the ice of Greenland and Antarctica has increased exponentially. Therefore, it can be said that it is difficult to find a piece of "pure land" in the world today where the soil lead content is not affected by human activities. [0003] my country's groundwater quality...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/544G01N33/531G01N33/532
Inventor 王利兵胥传来邢常瑞匡华刘丽强宋珊珊马文蔚赵媛
Owner JIANGNAN UNIV
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