74results about How to "Less prone to false positives" patented technology

The invention discloses a method for detecting food-borne pathogenic bacteria by using a colloidal gold nucleic acid test strip based on hyper-branched rolling cycle amplification and a kit for detecting Listeria monocytogenes and Salmonella, belonging to the technical field of biological detect. The method comprises the following main steps: (1) performing extraction and enzyme digestion on the DNA (deoxyribonucleic acid) of food-borne pathogenic bacteria; (2) designing an amplification primer and a nucleic acid probe sequence; (3) performing hyper-branched rolling cycle amplification reaction; (4) annealing and hybridizing; (5) preparing a nano gold probe; (6) preparing a colloidal gold nucleic acid test strip; and (7) detecting a sample. When the sample contains a target gene segment, both a T line and a C line of the colloidal gold nucleic acid test strip are formed into red lines; and if the sample does not contain the target gene segment, only the C line of the colloidal gold nucleic acid test strip is formed into a red line. According to the invention, food-borne pathogenic bacteria in the food sample can be detected quickly, specifically, sensitively, qualitatively and quantitatively; and the invention is simple in probe design, short in operation steps and favorable for popularization.

The invention discloses a kit for detecting pig pseudorabies. The kit comprises an assay plate coated with pig pseudorabies virus gE antigen, a rabbit anti-pig IgG antibody marked by HRP (horseradish peroxidase), fast coating buffer, fast blocking buffer, sample diluents, TMB substrate, stop buffer, 20 times wash solution, negative control, and a positive control. Through adopting pig pseudorabies virus specificity gE antigen with high purity and high activity expressed through gene engineering to coat the assay plate, and through adopting HRP-conjugate of rabbit anti-pig IgG monoclonal antibody containing HRP, the kit for detecting pig pseudorabies, provided by the invention, has the advantages of strong specificity, high sensibility, less susceptibility to misjudge of false positive or false negative and the like; moreover, the kit is simple in structure, low in detection cost, convenient and fast in operation and strong in timeliness, has a wide market prospect, and can create larger social benefits.

The invention discloses a test strip which is used for detecting porcine reproductive difficulty virus infectious disease pathogen, comprising a non-hygroscopic support layer and an adsorption layer which adheres to the support layer. The adsorption layer consists of a sample adsorption fiber layer, a gold antibody fiber layer, a cellulose film layer and a hygroscopic layer, and the four layers are spliced sequentially. The cellulose film layer has a contrast blot which contains anti-porcine/mouse IgG and at least one detect blot which contains porcine reproductive difficulty virus antibody that is one of anti-PRRSV, PPV and JEV monoclonal antibodies/polyclonal antibodies; the gold antibody fiber layer is adhered with gold polyclonal antibodies/monoclonal antibodies of anti-porcine reproductive difficulty virus corresponding to the virus antibody. When used for testing, the test strip has the advantages of strong specificity, high sensitivity and no need of additional device or reagent. The test strip can be operated by everyone and is suitable for fast identifying and diagnosing porcine reproductive difficulty virus infectious disease on site.

The invention discloses a Clenbuterol hydrochloride detection test paper and a detection method thereof. The Clenbuterol hydrochloride detection test paper comprises a support and fixing lining which is sequentially provided with a sample adsorption layer, a combination pad, a microporous siphon reaction layer and a water absorption layer; the combination pad is provided with a Clenbuterol hydrochloride antibody labeled by a trace particle and an antibody A labeled by the trace particle, and the antibody A is different from the Clenbuterol hydrochloride antibody; the microporous siphon reaction layer is sequentially provided with a blotting area and a detection area of Clenbuterol hydrochloride carrier protein solution, and the detection area comprises a detection solution blotting area and a control solution blotting area. Two lines occur in the detection area when the content of the Clenbuterol hydrochloride is more than 5 nanogram in a sample; and one line occurs in the detection area when the content of the Clenbuterol hydrochloride is less than 5 nanogram in the sample. The test paper has a detection mode and a reading manner equivalent to those of a sandwich method and low cost. The detection method is simple and rapid, and has simple operation.

The invention discloses a method for quickly identifying a food pathogenic bacteria subtype based on an asymmetric polymerase chain reaction (PCR) combined test strip platform and a kit, and belongs to the technical field of biological detection. The method comprises the main steps of: (1) extracting food pathogenic bacteria subtype DNA; (2) designing an amplification primer and a nucleic acid probe sequence; (3) performing amplification reaction based on asymmetric PCR; (4) preparing a nanogold probe; (5) preparing a colloidal gold nucleic acid test strip; (6) detecting a sample. According to the method, food pathogenic bacteria in food samples can be quickly, specially, sensitively, qualitatively and quantitatively detected and the special subtype of the pathogenic bacteria is determined; the probe is simply designed, the operation steps are simple and short, the product is a single chain, and the method is easy to detect and convenient to popularize.

The present invention relates to a test strip which is used for testing a kind of or a plurality of kinds of disease antibodies of porcine virus diarrhea; the test strip comprises a supporting layer and a reaction reagent carrier absorbing layer which is pasted on the supporting layer; the reaction reagent carrier absorbing layer comprises a testing end fiber layer, a fiber layer of absorbing gold-labeled SPA protein or gold-labeled antigen which corresponds to the antigen to be tested, and a cellulose layer, which are arranged at the sample end in sequence, and an absorbent material layer which is positioned at the handle end; the cellulose layer contains one, two or three testing print(s) which are printed by anyone, any two or three of the purified transmissible gastroenteritis virus TGEV solution, the porcine epidemic diarrhea virus PDEV solution and the porcine rotavirus RV, and the cellulose layer also contains the contrast prints which are printed by anyone, any two or three of the anti-SPA protein IgG solution of the sheep or the rabbit or the anti-TGEV, anti-PDEV, anti-RV IgG solution of the sheep or the rabbit; and the invention provides a test strip for testing a kind of or a plurality of kinds of disease antibodies of the porcine virus diarrhea, which has the advantages of accurate and rapid detection, convenient operation and low costs.

The invention relates to a colloid gold chromatographic test paper strip and a preparation method thereof for fast detecting sibutramine, belonging to the field of detection technique of biological immunoassay methods. The test paper strip comprises a scaleboard, a sample pad, a gold-marking binding pad, a coated film and a water absorbent pad, wherein the sample pad, the gold-marking binding pad, the coated film and the water absorbent pad are sequentially spliced on the scaleboard; the gold-marking binding pad is glass cellucotton for absorbing gold-marking antibodies of sibutramine ramifications; the coated film has a linear adelomorphic detection line T printed by carrier protein solution coupled by the sibutramine ramifications and a linear adelomorphic contrasting line C printed by goat anti-rabbit IgG solution, and the two lines are arranged in parallel. The test paper strip has strong specificity, high sensibility, strong timeliness detected minimum reaching 5ppb, visual, directly-viewing and accurate result display and wide scope of population, is simple, convenient and fast, does not need other reagents and instruments, can be operated in field, can determine detection results within 15 minutes when being added to detected sample solution, and saves cost.

The invention discloses a primer, a probe, a kit and a usage method of the kit. A primer, a probe and a kit for detecting a genotype of HPV (Human Papillomavirus) and a usage method of the kit can beused for simultaneously detecting multiple HPV subgenotypes; through using a real-time fluorescence PCR (Polymerase Chain Reaction) amplification technique, the nucleic-acid extraction only needs to be carried out once; a PCR detection reaction also needs to be carried out once in each group; the usage method of the kit is quick, simple and convenient; moreover, the specificity is good; other HPVsubgenotypes or other microbes and the like cannot be detected out; therefore, a false positive problem difficultly appears; moreover, the sensitivity is high, and can reach 400copies/ml; a detectionrange is 400copies/ml to 4.00E+09copies/ml, and a detection range is wide.

The invention discloses a clopidol colloidal gold chromatography detection test strip or card which comprises a lining board, a sample pad, a gold mark combined pad, a nitrocellulose membrane and a glass water-absorbing pad, wherein the sample pad, the gold mark combined pad, the nitrocellulose membrane and the glass water-absorbing pad are sequentially connected on the lining board; the gold mark combined pad is an anti-clopidol monoclonal antibody or a polyclonal antibody glass fiber cotton which is absorbed with a colloidal gold mark; and an invisible detection line printed by using a clopidol-carrier protein conjugate and an invisible comparison line printed by using goat anti-mouse IgG are positioned on the nitrocellulose membrane. The clopidol colloidal gold chromatography detection test strip and card disclosed by the invention has the advantages of high specificity, high sensitivity, easiness, convenience, high speed, high timeliness, vivid, visual and accurate result display, cost saving, wide application range and convenience for popularization.

The method comprises: partitioning the image of monitored area into several areas, setting up the correlative feature information about the set areas; said feature information comprise: the motion information threshold, and the monitored low speed target, high speed target and target over a defined speed; getting the motion vector from video encoder, and getting the motion information about the monitored area; comparing the obtained motion information with the preset motion information threshold to determine the final motion information; reporting the final motion information.

The invention discloses a high-precision natural gas leakage detector based on the Internet of things. The natural gas leakage detector comprises a natural gas leakage detector body and an LED display screen, the natural gas leakage detector body is internally provided with an MCU processor, the MCU processor is electrically connected with a power supply, the LED display screen and a control button, a sensor assembly is electrically connected with the MCU processor via a signal collecting and processing circuit, a wireless communication module is in signal connection with a remote monitoring terminal, and the MCU processor is electrically connected with a memory. The sensor assembly analyzes component concentration of natural gas, the MCU processor emits alarm signals when the component concentration reaches a certain value, false alarm is unlikely to occur, and alarming is more accurate; and at the same time, a photoelectric detector can increase leakage parameters that are processed by the MCU processor, so that the detector is more reliable and higher in precision.

The invention provides a colloidal gold chromatography test strip for quickly detecting lead ions and belongs to the technical field of immunology. The colloidal gold chromatography test strip consists of a lining plate and a sample pad, a gold labeled combination pad, a coating film and an absorbent pad which are connected with the lining plate sequentially, wherein the gold labeled combination pad is glass fiber cotton for absorbing lead ion gold labeled antibodies; a linear hidden detection T line printed by using solution in which the lead ions are coupled with a carrier protein and a linear hidden contrast line C printed by using goat anti mouse IgG are formed on the coating film sequentially; and the two lines are arranged in parallel. The colloidal gold chromatography test strip for quickly detecting the lead ions has high specificity and high sensitivity, can detect lead ion residues more quickly, sensitively and simply, is vivid, intuitive, accurate, simple and clear in result judgment, avoids manmade misjudgment such as false positive, false negative and the like, saves cost, is convenient to popularize and has wide market prospect, outstanding economic and social benefits and a wide application range.

The invention discloses a millimeter-wave life detection alarm system and an alarm method for preventing children from being left behind in a vehicle, wherein the alarm system comprises a millimeter wave sensor mounted on the inner side of the vehicle body, An alarm control module electrically connected to the millimeter wave sensor and an alarm module electrically connected to the alarm control module, the millimeter wave sensor comprising a transmitting module and a receiving module, the alarm control module comprising a system control switch and a signal processing module. As soon as that invention detect the existence of life in the vehicle, the invention will send out an alarm sound to remind the surrounding personnel, and effectively solve the problem that the child, especially the 1-4-year-old infants stay in the car, lack of self-help ability, difficult to sound a distress or alarm technical problems.

The invention discloses a quantum dot test strip for rapidly detecting trace fipronil and a preparation method of the quantum dot test strip. According to the test strip, a bottom layer is a supporting layer, a middle layer is an adsorption layer, a protecting layer is fixed on the adsorption layer, the adsorption layer comprises an adsorption fiber layer, a quantum dot labeled antibody fiber layer, a cellulose membrane layer and a water absorbing material layer at a handle end sequentially from the test end, wherein a cellulose membrane is coated with fipronil coupled carrier protein to serveas a detection line, and a quality control line printed by goat anti or rabbit anti-mouse IgG, goat anti rabbit IgG antibody or SPA is also arranged. A test indicates that the test strip has higher specificity and higher sensitivity, limit of visual detection is 1 ng/mL, and the test strip is free of cross reaction with other pesticides or veterinary drugs and has quite great significance in theaspects of guaranteeing food safety and protecting health of consumers.

The invention discloses a primer, a probe and a kit for detecting the microorganism in a urogenital canal and a use method of the kit. By the primer, the probe and the kit for detecting the microorganism in the urogenital canal and the use method of the kit, the chlamydia trachomatis, the ureaplasma urealyticum and the gonococcus can be detected simultaneously, the extraction of the nucleic acid is only conducted and only one step of the PCR detection reaction is conducted through the real-time fluorescence PCR amplification technique, the use method of the kit is simple and rapid, the specificity is good, the pathogene microorganisms of the chlamydia trachomatis, the ureaplasma urealyticum and the gonococcus are not detected, the problem of false positive result is not easy to cause, thesensitivity is as high as 400 copies/ml, the detection range is 400 copies/ml to 4.00E+09 copies/ml, and the detection range is wide.

The invention discloses pseudorabies antibody blocking test paper, composed of a supporting plate, an antigen pad, a gold labeling pad, detection film and a water absorption pad. The antigen pad absorbs pseudorabies virus detection antigens. The gold labeling pad absorbs colloidal gold labeled anti-pseudorabies virus gE and gB monoclonal antibodies mAb1 and mAb2. Virulent detection line T1''|'', vaccine virus detection line T2''|'' and quality control line C''|'' prints are set on the detection film. The virulent detection line T1 is an anti-pseudorabies virus gE protein monoclonal antibody mAb3 print. The vaccine virus detection line T2 is an anti-pseudorabies virus gB protein monoclonal antibody mAb4 print. The quality control line C is a rabbit anti-mouse IgG antibody pAb1 or staphylococcus aureus SPA print ''|''. According to the test paper, rapid detection and real-time detection of pseudorabies virus infection and immune antibodies are realized, infection and immune detection andimmune evaluation of a vaccine immune effect are realized, operation is simple, and the test paper is easy to popularize and apply in a wide range.

The invention discloses a method of screening transgene cole through dipping seeds into kanamycin solution. The cole seeds having undergone transgene processing are dipped into the 0.18-0.30 percent kanamycin solution for processing for 12-24 hours and then are sowed in the soil which does not contain kanamycin, and the green seedlings growing normally are screened as the transgene plants after 2-3 days. The method of screening transgene cole is applicable to screening of a large amount of transgene cole seeds with simplicity, quickness, high efficiency, and reliability.

The invention discloses a colloidal gold chromatography test strip for quickly detecting sibutramine and derivative thereof and a preparation method thereof. The test strip consists of a lining plate and a sample pad, a gold label bonding pad, a coating film and a water absorption pad engaged on the lining plate in turn, wherein on the coating film, a straight detection line T is coated by using carrier protein solution coupled with the sibutramine and the derivative thereof, and a straight contrast line C is coated by using goat-anti-mouse IgG solution. The method comprises the following steps of: preparing artificial conjugated antigens; preparing monoclonal antibodies of the sibutramine and the derivative thereof; preparing colloidal gold solution; and obtaining a colloidal gold marker and the gold label bonding pad for resisting the monoclonal antibodies of the sibutramine and the derivative thereof. The test strip has the advantages of strong specificity, high sensitivity, strong timeliness and vivid and accurate result display, and is simple, convenient and quick.

The invention discloses hepatitis C virus recombination protein, characterized in that: the hepatitis C virus recombination protein is fusion protein formed by fusing hepatitis C virus main antigenic determinant and hepatitis C virus core protein single chain antibody, and the amino acid sequence is represented as SEQ ID No.1. According to the invention, gene engineering recombination technology is utilized, the hepatitis C virus main antigenic determinant-core protein single chain antibody fusion protein is expressed in escherichia coli system, and the advantages of short production period, high yield and low cost are achieved. The recombination protein can be used as a part of an immunodiagnostic kit for hepatitis C virus.

The invention discloses a blocking test paper for swine fever antibody. The test paper is composed of a support plate, an antigen pad, a gold standard pad, a detection membrane and an absorbent pad, wherein the antigen pad absorbs the detection antigen of swine fever virus, the gold standard pad absorbs the colloidal gold labeled anti-swine fever virus monoclonal antibody mAb1, the detection membrane contains "|" blots of a detection line T and a quality control line C, the detection line T is the blot of an anti-swine fever monoclonal antibody mAb2 or an anti-swine fever polyclonal antibody pAb1, and the quality control line C is the blot of an anti-mouse IgG antibody pAb2 or a staphylococcus aureus SPA. The test paper provided by the invention realizes the rapid detection of neutralizingantibody of swine fever virus, can realize real-time monitoring of the maternal antibody and immune antibody level of the swine fever as well as the immune evaluation of the vaccine immune effect, and is simple in operation and applicable for everyone, which can well meet the needs of different levels of personnel, is easy to promote and apply in a wide range, and has broad market prospects and large economic and social benefits.

The invention provides a gas igniter integrated device. The gas igniter integrated device comprises a transformer sleeve, a gas pipe connected with the transformer sleeve and a casing pipe connected with one side of the gas pipe and further comprises an automatic ignition device, an ignition transformer, a flame monitoring device, an input and output port and a programmable logic controller (PLC) control circuit. The automatic ignition device is arranged in the transformer sleeve and connected with the PLC control circuit through the input and output port; and the ignition transformer is arranged in the transformer sleeve, connected with a relay of the automatic ignition device, and controlled by the PLC control circuit. The flame monitoring device is arranged in the casing pipe and composed of three parts including a gas burner, an ignition electrode and an ion electrode, the gas burner is connected with the gas pipe through a valve, the ignition electrode is connected with the ignition transformer, and the ion electrode is connected with the automatic ignition device. The ignition transformer can generate thousands of volts of high voltage, simultaneously a short circuit protective circuit and an open circuit protective circuit are provided; flame is monitored in real time; and the gas igniter integrated device is high temperature resistant, strong acid resistant, oxidation resistant, compact in structure and convenient to use.

The present invention discloses a phenobarbital quick detection test paper strip. It contains bottom layer as supporting layer, intermediate layer as adsorption layer and protective layer fixed on the adsorption layer, Said adsorption layer successively includes test end adsorption fibre layer, adsorption gold-labelled amtibody fibre layer, cellulose membrane layer and water-absorbing material layer of handle end. Besides, said invention also provides the concrete structure on the cellulose membrane layer, said structure include linear detection blotting and linear reference blotting.

The invention relates to a colloidal gold immunochromatographic test paper capable of quickly detecting a soybean Kunitz trypsin inhibiting factor and a preparation method. The test paper detects a KTI (Key Technology Index) based on a double-antibody sandwich principle, the test paper container comprises a support layer, an adsorption layer and a protection layer, and the adsorption layer is sequentially provided with a sample cushion, a binding cushion, a cellulose membrane and a water adsorption cushion from a test end. The binding cushion absorbs a specific antibody with the KTI, the cellulose membrane is provided with a detection line used for a KTI anti-cleaved caspase rabbit polyclonal antibody solution spray point and a quality control line used for an anti-mouse IgG (immunoglobulin G) antibody or a rabbit anti-mouse IgG antibody solution spray point, and the KTI specific antibody adopts a KTI murine monoclonal antibody marked by colloidal gold nano-particles. The immunochromatographic test paper provided by the invention is strong in specificity, high in flexibility, convenient, intuitionistic and accurate to detect, low in cost, wide in scope of application, and easy for popularization and application.

The invention provides a vehicle end power battery lithium precipitation online monitoring method. The method comprises the following steps: acquiring battery voltage data in a standing state after a battery is charged in a vehicle end or battery swap station scene, and calculating the voltage deviation degree at each moment according to the acquired voltage data; and when the calculated voltage deviation degree corresponding to a certain moment is greater than the voltage deviation degree at the previous moment, triggering the calculation of the voltage change trend of the battery, and judging whether lithium is separated from the battery or not according to the calculated voltage change trend. By means of the method, the voltage data of the battery can be accurately acquired and the voltage deviation degree can be calculated by utilizing the standing state after the battery is charged in a vehicle end or battery swap station scene, so that the calculation of the voltage change trend can be triggered according to the voltage deviation condition, and the nondestructive detection of the lithium precipitation of the battery core can be accurately and efficiently realized. The method is short in time, low in cost, high in efficiency, good in accuracy and relatively high in practical application value.

The invention relates to a small tower climbing monitoring device. The small tower climbing monitoring device consists of a plurality of touch detecting terminals which are mounted on climbing nails and a monitoring master control module. Each touch detecting terminal comprises a fastening structure and a transmission structure. When a foot of a tower climber steps on a transmission structure, thetransmission structure rotates downwards by taking a through hole as an axis and presses a spring and limit switch contact. When a limit switch is pressed down and switched on, the monitoring mastercontrol module detects touch information through a data line. When two and more touch detecting terminals are touched simultaneously, the situation that a person climbs a tower is determined. When thepositions of the detecting devices touched in sequence is from bottom to top within specified time, the situation that the person climbs up the tower is determined; and when the positions of the detecting devices touched in sequence is from top to bottom within specified time, the situation that the person climbs down the tower is determined. Compared with the prior art, a touch situation is detected by adopting a mechanical structure, so that the small tower climbing monitoring device is higher in structural stability and is not prone to false alarm.

The present invention provides a visual detection method for PCR (polymerase chain reaction) amplification and an endpoint. The method comprises the steps as follows: adding a visible light dye with aconcentration of 0.1-0.3 mM into an amplification system before the PCR amplification reaction, and judging the reaction result by observing the color change of a complex formed by the visible lightdye and substances in the reaction system. The detection phase visually determines the detection result. The method is simple and easy, avoids cross-contamination caused by the cover opening, avoids harm to the human body, and reduces the detection cost.