Primer, probe and kit for detecting genotype of HPV (Human Papillomavirus) and usage method of kit

Abstract

The invention discloses a primer, a probe, a kit and a usage method of the kit. A primer, a probe and a kit for detecting a genotype of HPV (Human Papillomavirus) and a usage method of the kit can beused for simultaneously detecting multiple HPV subgenotypes; through using a real-time fluorescence PCR (Polymerase Chain Reaction) amplification technique, the nucleic-acid extraction only needs to be carried out once; a PCR detection reaction also needs to be carried out once in each group; the usage method of the kit is quick, simple and convenient; moreover, the specificity is good; other HPVsubgenotypes or other microbes and the like cannot be detected out; therefore, a false positive problem difficultly appears; moreover, the sensitivity is high, and can reach 400copies / ml; a detectionrange is 400copies / ml to 4.00E+09copies / ml, and a detection range is wide.

Description

technical field [0001] The invention relates to the field of molecular biology detection, in particular to a primer and a probe for HPV typing detection, a kit and a method for using the kit. Background technique [0002] HPV (Human papillomavirus, HPV, human papillomavirus) is a kind of non-enveloped double-stranded circular DNA virus with small molecular weight, which obligately infects and parasitizes the epithelial cells of human reproductive organs and other tissues and organs. Clinically, HPV can be divided into high-risk and low-risk types according to the different pathogenicity or cancer risk of different HPV subtypes. Low-risk HPV mainly causes exophytic wart-like lesions on the anus skin, male external genitalia, female labia, urethral meatus, lower vagina, and low-grade cervical intraepithelial neoplasia. In addition to causing external genital warts, high-risk HPV can cause external genital cancer, cervical cancer and high-grade cervical intraepithelial neoplas...

AI Technical Summary

Problems solved by technology

However, traditional detection methods are often aimed at the detection of a single HPV subtype, and the detection process of a mixture of multiple HPV subtypes is cumbersome, time-consuming, and has poor specificity and low sensitivity.

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