Multilayer dry slides for measuring content of high-density lipoprotein (HDL)

A high-density lipoprotein and multi-layer film technology, applied in the field of in vitro clinical reagents, can solve the problems of complicated operation, difficult clinical laboratory deployment, inconvenient storage and transportation, etc., and achieves easy storage and transportation, wide application range, and strong anti-interference ability. Effect

Pending Publication Date: 2019-08-27
BEIJING INSTITUTE OF PETROCHEMICAL TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The commonly used method in China is the precipitation method. The commonly used precipitants include: heparin-calcium reagents, glucose sulfate-magnesium reagents, phosphotungstic acid-magnesium reagents, etc. The precipitants will coagulate lipoproteins other than HDL and centrifuge them. Precipitation, the determination of HDL content in the supernatant according to the e

Method used

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  • Multilayer dry slides for measuring content of high-density lipoprotein (HDL)
  • Multilayer dry slides for measuring content of high-density lipoprotein (HDL)
  • Multilayer dry slides for measuring content of high-density lipoprotein (HDL)

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Effect test

Embodiment 1

[0033] Embodiment 1: The components of the high-density lipoprotein membrane dry sheet reagent of the present invention are as follows:

[0034] Diffusion layer reagents:

[0035] Gelatin 50-150g / L

[0036] Peroxidase 0.01-1.0g / L

[0037] Cholesterol lipase 0.03-2.5g / L

[0038] Cholesterol oxidase 0.01-2.5g / L

[0039] Ascorbate oxidase 0.01-2.0g / L

[0040] 2-(3,5-Dimethoxy-4-hydroxyphenyl)-4,5-bis-(4-dimethylaminophenyl)imidazole 0.01-1.5g / L

[0041] Phosphocholine buffer solution pH=6.0-7.0

[0042] Reagent Layer Reagent:

[0043] Barium sulfate or TiO2 or barium titanate 500-2200g / L

[0044] Phosphotungstic acid 0.5-10.0g / L

[0045] Magnesium chloride 1.0-10.0g / L

[0046] Cellulose acetate 8-1500g / L

[0047] Phosphocholine surfactant 0.3-10g / L

[0048] Polyethylene glycol monooleate 0.2-10g / L

Embodiment 2

[0049]Embodiment 2: The performance test of the high-density lipoprotein multilayer film dry sheet of the present invention is as follows:

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Abstract

The invention relates to multilayer dry slides for measuring the content of high-density lipoprotein (HDL). The multilayer dry slides are characterized in that the multilayer dry slides comprise a lower dry slide base (5), a supporting layer (3), a reagent layer (2), a diffusion layer (1) and an upper dry slide base (4), wherein the lower dry slide base (5) is integrally bonded with the upper dryslide base (4), and a loading hole (6) and a test hole (7) are formed in the same positions up and down. The multilayer dry slides involved in the invention have good specificity and strong anti-interference capacity, can resist the interference of hemoglobin, bilirubin and ascorbic acid, are suitable for full-automatic analyzers, and have a relatively large clinical application value; meanwhile,the multilayer dry slides are easy to store and transport, reasonable in design, strong in practicability and wide in application range.

Description

technical field [0001] The invention belongs to the technical field of in vitro clinical reagents, and in particular relates to a dry biomedical dry sheet for measuring high-density lipoprotein content, which can be used to detect the content of high-density lipoprotein in serum and plasma. Background technique [0002] In clinical testing, compared with traditional wet chemical methods, dry chemical methods have the advantages of simple operation, fast detection speed, no need to use liquid reagents, and convenient transportation and storage. Generally speaking, the research on dry chemical method in China started relatively late, and the researched technology is not yet mature enough. Therefore, the development of dry chemical reagents with independent intellectual property rights is of great significance to the promotion and application of dry chemical analysis technology in China. Lipids such as cholesterol will cooperate with apoproteins to form lipoproteins in serum, a...

Claims

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Application Information

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IPC IPC(8): C12Q1/60C12Q1/28C12Q1/44C12Q1/26
CPCC12Q1/60C12Q1/28C12Q1/44C12Q1/26
Inventor 靳海波杨索和郭晓燕刘月娇何广湘
Owner BEIJING INSTITUTE OF PETROCHEMICAL TECHNOLOGY
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