Method for detecting tetracycline and clenbuterol by carbon dot-rhodamine B bifluorescence system proportional fluorescence probe

A technology of fluorescent probe and tetracycline, which is applied in the field of chemical analysis and detection, can solve problems such as the impact on the health of the food population, antibiotic residues, and the impact on detection accuracy, and achieve high sensitivity, good specificity, and strong specificity
CN110441280APending Publication Date: 2019-11-12云南健牛环境监测有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
云南健牛环境监测有限公司
Publication Date
2019-11-12

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Abstract

The invention discloses a method for detecting tetracycline and clenbuterol by a carbon dot-rhodamine B bifluorescence system proportional fluorescence probe. In the method, phenylboronic acid and 3-aminopropyl triethoxy silane are used as a carbon source and a silicon source; boron and silicon doped fluorescence carbon dots are compounded by a hydrothermal method; a bifluorescence system is formed by the compounded boron and silicon doped fluorescence carbon dots and rhodamine B, three transmission wavelengths of 414nm, 578nm and 670nm are generated, and a double proportion fluorescence probedetection system is formed by the double wavelength of the boron and silicon doped fluorescence carbon dots and rhodamine B serving as reference fluorescence. The tetracycline and clenbuterol respectively have linear fluorescence quenching and fluorescence sensibilization effects on the double proportion fluorescence probe detection system, and thus the proportional fluorescence probe detection method for detecting the tetracycline and clenbuterol is built. The method has the characteristics of being high in sensitivity, strong in specificity, simple to operate and rapid.
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Description

technical field

[0001] The invention relates to the technical field of chemical analysis and detection, in particular to a method for detecting tetracycline and clenbuterol with a carbon dot-rhodamine B dual fluorescence system proportional fluorescent probe. Background technique

[0002] Tetracycline antibiotics are hydrogenated tetracene ring derivatives with strong ultraviolet absorption and fluorescence properties. It is an acid-base amphoteric substance, easily soluble in acidic or alkaline solutions, and relatively stable in weakly acidic solutions. Tetracycline antibiotics are broad-spectrum bacteriostatic agents that have a bactericidal effect at high concentrations. They have a good inhibitory effect on Gram-positive bacteria, Gram-negative bacteria, Rickettsia and viral viruses. , due to its relatively low price, is one of the antibiotics frequently used in the current breeding industry. Feeding animals with antibiotic-added feed will cause antibiotic residues in ...

Claims

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