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Method for detecting tetracycline and clenbuterol by carbon dot-rhodamine B bifluorescence system proportional fluorescence probe

A technology of fluorescent probe and tetracycline, which is applied in the field of chemical analysis and detection, can solve problems such as the impact on the health of the food population, antibiotic residues, and the impact on detection accuracy, and achieve high sensitivity, good specificity, and strong specificity

Pending Publication Date: 2019-11-12
云南健牛环境监测有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Tetracycline antibiotics are broad-spectrum bacteriostatic agents that have a bactericidal effect at high concentrations. They have a good inhibitory effect on Gram-positive bacteria, Gram-negative bacteria, Rickettsia and viral viruses. , due to its relatively low price, it is one of the commonly used antibiotics in the current breeding industry. Feeding animals with feed added with antibiotics will cause antibiotic residues in animal foods, which will have a health impact on the people who eat them
There have been many reports on the use of doped carbon dots as fluorescent probes. Fluorescence analysis has the advantages of simplicity, rapidity, high sensitivity, and no need for complicated instruments and equipment, but it is also susceptible to external environment, detection substrates, and photobleaching. , which affects the detection accuracy

Method used

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  • Method for detecting tetracycline and clenbuterol by carbon dot-rhodamine B bifluorescence system proportional fluorescence probe
  • Method for detecting tetracycline and clenbuterol by carbon dot-rhodamine B bifluorescence system proportional fluorescence probe
  • Method for detecting tetracycline and clenbuterol by carbon dot-rhodamine B bifluorescence system proportional fluorescence probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: Determination of tetracycline and clenbuterol in pork The operation steps are as follows:

[0023] 1. Carbon dots are prepared by the following method: Weigh 1.0~5.0g of phenylboronic acid, add it to 100~150 mL of absolute ethanol, ultrasonicate for 5~10 minutes to form a colorless and transparent solution, add 4~8g of 3-aminopropyl tris Ethoxysilane, 2 ~ 4 mL ammonia water, ultrasonic 10min to form a uniform solution, and transferred to a polytetrafluoroethylene reaction kettle, heated at 180 ℃ for 8 ~ 10h, after natural cooling, centrifuged at 10000r / min for 10min, first used Filter with a pore size of 0.22 μm membrane, and then use a dialysis bag with a molecular weight cut-off of 3000-3500 Da for dialysis treatment for 24 hours to obtain fluorescent carbon dots;

[0024] 2. Preparation of carbon dots-rhodamine B fluorescent system: Take 1 mL of the above synthetic fluorescent carbon dots and mix with 0.1-0.2 mL of 0.1-0.5 mg / mL rhodamine B absolute eth...

Embodiment 2

[0036] Example 2: Determination of tetracycline and clenbuterol in chicken The operation steps are as follows:

[0037] 1. Carbon dots are prepared by the following method: Same as Example 1;

[0038] 2. Preparation of carbon dot-rhodamine B fluorescence system: same as Example 1;

[0039] 3, tetracycline standard curve making: with embodiment 1;

[0040] 4. Preparation of clenbuterol standard curve: same as in Example 1;

[0041] 5. Determination of tetracycline and clenbuterol in chicken samples:

[0042] (1) Extraction: same as Example 1;

[0043] (2) purification: same as embodiment 1;

[0044] (3) Determination of tetracycline and clenbuterol: the operation was the same as in Example 1, the content of tetracycline calculated from the regression equation was 1.23 μg / kg, and the relative standard deviation was 1.9%; clenbuterol was not detected.

Embodiment 3

[0045] Example 3: Determination of tetracycline and clenbuterol in milk samples The operation steps are as follows:

[0046] 1. Carbon dots are prepared by the following method: Same as Example 1;

[0047] 2. Preparation of carbon dot-rhodamine B fluorescence system: same as Example 1;

[0048] 3, tetracycline standard curve making: with embodiment 1;

[0049] 4. Preparation of clenbuterol standard curve: same as in Example 1;

[0050] 5. Determination of tetracycline and clenbuterol in milk samples:

[0051] (1) Milk sample preparation: take 5 mL of milk, add 10 mL of methanol-water-formic acid (28:72:0.1, v / v) extract and 1 mL of 4% trichloroacetic acid in acetonitrile, vortex for 3 min, and sonicate for 5 min, centrifuge at 10,000r / min for 10 min, take the supernatant, and store it in a refrigerator at 4°C in the dark until analysis and determination;

[0052] (2) Sample determination: The operation was the same as in Example 1. The tetracycline was calculated from the ...

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Abstract

The invention discloses a method for detecting tetracycline and clenbuterol by a carbon dot-rhodamine B bifluorescence system proportional fluorescence probe. In the method, phenylboronic acid and 3-aminopropyl triethoxy silane are used as a carbon source and a silicon source; boron and silicon doped fluorescence carbon dots are compounded by a hydrothermal method; a bifluorescence system is formed by the compounded boron and silicon doped fluorescence carbon dots and rhodamine B, three transmission wavelengths of 414nm, 578nm and 670nm are generated, and a double proportion fluorescence probedetection system is formed by the double wavelength of the boron and silicon doped fluorescence carbon dots and rhodamine B serving as reference fluorescence. The tetracycline and clenbuterol respectively have linear fluorescence quenching and fluorescence sensibilization effects on the double proportion fluorescence probe detection system, and thus the proportional fluorescence probe detection method for detecting the tetracycline and clenbuterol is built. The method has the characteristics of being high in sensitivity, strong in specificity, simple to operate and rapid.

Description

technical field [0001] The invention relates to the technical field of chemical analysis and detection, in particular to a method for detecting tetracycline and clenbuterol with a carbon dot-rhodamine B dual fluorescence system proportional fluorescent probe. Background technique [0002] Tetracycline antibiotics are hydrogenated tetracene ring derivatives with strong ultraviolet absorption and fluorescence properties. It is an acid-base amphoteric substance, easily soluble in acidic or alkaline solutions, and relatively stable in weakly acidic solutions. Tetracycline antibiotics are broad-spectrum bacteriostatic agents that have a bactericidal effect at high concentrations. They have a good inhibitory effect on Gram-positive bacteria, Gram-negative bacteria, Rickettsia and viral viruses. , due to its relatively low price, is one of the antibiotics frequently used in the current breeding industry. Feeding animals with antibiotic-added feed will cause antibiotic residues in ...

Claims

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Application Information

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IPC IPC(8): G01N21/64C09K11/65C01B32/15B82Y20/00B82Y40/00
CPCG01N21/643C09K11/65C01B32/15B82Y20/00B82Y40/00G01N2021/6432
Inventor 陈子昭万洋母昭
Owner 云南健牛环境监测有限公司
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