Method for detecting tetracycline and clenbuterol by carbon dot-rhodamine B bifluorescence system proportional fluorescence probe

A technology of fluorescent probe and tetracycline, which is applied in the field of chemical analysis and detection, can solve problems such as the impact on the health of the food population, antibiotic residues, and the impact on detection accuracy, and achieve high sensitivity, good specificity, and strong specificity

Pending Publication Date: 2019-11-12
云南健牛环境监测有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Tetracycline antibiotics are broad-spectrum bacteriostatic agents that have a bactericidal effect at high concentrations. They have a good inhibitory effect on Gram-positive bacteria, Gram-negative bacteria, Rickettsia and viral viruses. , due to its relatively low price, it is one of the commonly used antibiotics in the current breeding industry. Feeding animals with feed added with antibiotics will cause antibiotic residues

Method used

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  • Method for detecting tetracycline and clenbuterol by carbon dot-rhodamine B bifluorescence system proportional fluorescence probe
  • Method for detecting tetracycline and clenbuterol by carbon dot-rhodamine B bifluorescence system proportional fluorescence probe
  • Method for detecting tetracycline and clenbuterol by carbon dot-rhodamine B bifluorescence system proportional fluorescence probe

Examples

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Example Embodiment

[0022] Example 1: The procedure for determining the content of tetracycline and clenbuterol in pork is as follows:

[0023] 1. The carbon dots are prepared by the following method: Weigh 1.0~5.0g phenylboronic acid, add it to 100~150 mL absolute ethanol, sonicate for 5~10 min to form a colorless and transparent solution, add 4~8g 3-aminopropyl triacetate Ethoxysilane, 2~4 mL ammonia water, ultrasonic for 10min to form a uniform solution, and transfer to the polytetrafluoroethylene reactor, heating at 180℃ for 8~10 h, after natural cooling, centrifugation at 10000r / min for 10 min, use first The pore size is 0.22μm filter membrane, and then the dialysis bag with a molecular weight cut-off of 3000~3500Da is used for dialysis treatment for 24h to obtain fluorescent carbon dots;

[0024] 2. Preparation of carbon dots-rhodamine B fluorescence system: take 1 mL of the above synthetic fluorescent carbon dots and mix with 0.1-0. 5mg / mL rhodamine B absolute ethanol solution 0.1-0.2 mL, dilut...

Example Embodiment

[0036] Example 2: The procedure for determining the content of tetracycline and clenbuterol in chicken is as follows:

[0037] 1. The carbon dots were prepared by the following method: the same as in Example 1;

[0038] 2. Preparation of carbon dot-rhodamine B fluorescence system: same as in Example 1;

[0039] 3. Production of Tetracycline Standard Curve: Same as Example 1;

[0040] 4. Clenbuterol standard curve production: same as Example 1;

[0041] 5. Determination of Tetracycline and Clenbuterol in Chicken Samples:

[0042] (1) Extraction: same as Example 1;

[0043] (2) Purification: same as Example 1;

[0044] (3) Determination of Tetracycline and Clenbuterol: The operation is the same as in Example 1. The content of tetracycline is 1.23 μg / kg calculated from the regression equation, and the relative standard deviation is 1.9%; Clenbuterol is not detected.

Example Embodiment

[0045] Example 3: The procedure for determining the content of tetracycline and clenbuterol in milk samples is as follows:

[0046] 1. The carbon dots were prepared by the following method: the same as in Example 1;

[0047] 2. Preparation of carbon dot-rhodamine B fluorescence system: same as in Example 1;

[0048] 3. Production of Tetracycline Standard Curve: Same as Example 1;

[0049] 4. Clenbuterol standard curve production: same as Example 1;

[0050] 5. Determination of Tetracycline and Clenbuterol in Milk Samples:

[0051] (1) Milk sample preparation: Take 5 mL of milk, add 10 mL of methanol-water-formic acid (28:72:0.1, v / v) extract and 1 mL of 4% trichloroacetic acid acetonitrile solution, vortex for 3 min, and ultrasonic 5 min, centrifuge at 10000r / min for 10 min, take the supernatant and store in a refrigerator at 4 ℃ in the dark for analysis and determination;

[0052] (2) Sample determination: The operation is the same as in Example 1. The tetracycline is calculated from th...

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Abstract

The invention discloses a method for detecting tetracycline and clenbuterol by a carbon dot-rhodamine B bifluorescence system proportional fluorescence probe. In the method, phenylboronic acid and 3-aminopropyl triethoxy silane are used as a carbon source and a silicon source; boron and silicon doped fluorescence carbon dots are compounded by a hydrothermal method; a bifluorescence system is formed by the compounded boron and silicon doped fluorescence carbon dots and rhodamine B, three transmission wavelengths of 414nm, 578nm and 670nm are generated, and a double proportion fluorescence probedetection system is formed by the double wavelength of the boron and silicon doped fluorescence carbon dots and rhodamine B serving as reference fluorescence. The tetracycline and clenbuterol respectively have linear fluorescence quenching and fluorescence sensibilization effects on the double proportion fluorescence probe detection system, and thus the proportional fluorescence probe detection method for detecting the tetracycline and clenbuterol is built. The method has the characteristics of being high in sensitivity, strong in specificity, simple to operate and rapid.

Description

technical field [0001] The invention relates to the technical field of chemical analysis and detection, in particular to a method for detecting tetracycline and clenbuterol with a carbon dot-rhodamine B dual fluorescence system proportional fluorescent probe. Background technique [0002] Tetracycline antibiotics are hydrogenated tetracene ring derivatives with strong ultraviolet absorption and fluorescence properties. It is an acid-base amphoteric substance, easily soluble in acidic or alkaline solutions, and relatively stable in weakly acidic solutions. Tetracycline antibiotics are broad-spectrum bacteriostatic agents that have a bactericidal effect at high concentrations. They have a good inhibitory effect on Gram-positive bacteria, Gram-negative bacteria, Rickettsia and viral viruses. , due to its relatively low price, is one of the antibiotics frequently used in the current breeding industry. Feeding animals with antibiotic-added feed will cause antibiotic residues in ...

Claims

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Application Information

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IPC IPC(8): G01N21/64C09K11/65C01B32/15B82Y20/00B82Y40/00
CPCB82Y20/00B82Y40/00C01B32/15C09K11/65G01N21/643G01N2021/6432
Inventor 陈子昭万洋母昭
Owner 云南健牛环境监测有限公司
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