32 results about "Dual fluorescence" patented technology

The invention discloses a dual fluorescent quantitative PCR (polymerase chain reaction) primer probe combination for identifying a PRV (pseudorabies virus) vaccine strain and a wild strain. The dual fluorescent quantitative PCR primer probe combination comprises two pairs of primers gB-F and gB-R, gE-F and gE-R, and probes gB-probe and gE-probe, wherein the two pairs of primers are used for specifically amplifying a PRV gB gene and a PRV gE gene; through existence of real-time fluorescence PCR amplification curves of the PRV gB gene and the gE gene, a PRV vaccine strain (gE gene deletion) and a wild strain can be rapidly, specifically, sensitively and stably identified, technical support can be provided for porcine pseudorabies epidemic disease monitoring, and thorough purification of the PRV in a live pig breeding link is facilitated.

The application discloses an aggregation-induced emission compound with double fluorescence emission characteristics and a preparation method and application thereof, and the proportional fluorescence probe has a structure of general formula I. The probe molecule has a polar response group tetraphenyl ethylene as a fluorescence report unit, introduces a hydrophilic oxygen atom, connects an alpha carbon of an acetamide, and connects a polar detection sensitization group Tag chain on an amino group of the acetamide. The probe molecule improves the conjugation degree of oxygen atom lone pair electrons and a tetraphenyl ethylene pi electron system, so that the probe molecule simultaneously produces n→pi* and pi→pi* molecular transitions, and a proportional fluorescence probe which can sensitively respond to polarity change is constructed. The fluorescence probe designed in the application has the advantages that the fluorescence probe is not easily interfered by probe concentration, a detection environment and light bleaching, and experiments prove that the proportional fluorescence intensity of the fluorescence probe can sensitively respond to polarity, and the proportional fluorescence intensity and water content are linearly fitted, so that the purposes of detecting water content in an organic reagent and alcohol content in wine are achieved.

The invention discloses a preparation method of an Eu-Tb-In-MOF material based on a dual fluorescence emission source type, and the preparation method specifically comprises the following steps: under a closed condition, uniformly mixing terbium nitrate hexahydrate, europium nitrate hexahydrate, indium nitrate pentahydrate, an organic ligand, N, N-dimethylformamide and acetonitrile, then adjusting the pH value of a mixed solution, and carrying out solvothermal reaction, washing, filtering and drying to obtain the Eu-Tb-In-MOF material. The invention also discloses an application of the Eu-Tb-In-MOF material in identification of heavy metal ions Cr in a water environment. The Cr (VI) ions in the water environment are detected through fluorescence quenching sensing, excellent high selectivity is shown, and interference of other single and mixed anions and cations in the water environment can be resisted.

The invention discloses a bifluorescence reporter plasmid, construction of the plasmid and application of the bifluorescence reporter plasmid in detection of bacterial extracellular itaconic acid level. The reporter plasmid is composed of a resistance gene, a promoter, a first reporter gene, a terminator, a transcriptional regulation factor BM590RS11055, a bidirectional promoter and a second reporter gene. The promoter Ptac controls the expression of the first reporter gene mCherry, is used for detecting the plasmid background expression level, and can eliminate the influence of the plasmid copy number on the itaconic acid level detection result; the bidirectional promoter PIct controls the expression of the second reporter gene EGFP and is used for detecting the extracellular itaconic acid level, the expression of the first reporter gene and the expression of the second reporter gene are not interfered with each other, and the detection result is more accurate and more reliable. The bifluorescence reporter plasmid constructed by the invention can be used for detecting the extracellular itaconic acid level of bacteria, the operation is simple, and the result is reliable.

The present disclosure provides a dual fluorescence and visible light image fusion method and device, electronic equipment and medium, relating to the field of image processing technology. The present disclosure specifically implements the following scheme: simultaneously acquiring a visible light image and a fluorescence original image of a target scene; obtaining a conversion hue, a first saturation and a first brightness based on the fluorescence original image, wherein the conversion hue is a value obtained by linearly converting the hue value of the fluorescence original image; identifying a green component and a blue component based on the conversion hue and the fluorescence original image, and obtaining a mask image having information of the green component and the blue component; and obtaining a fused image based on the visible light image, the mask image, the first saturation and the first brightness, thereby improving the fusion effect of the visible light image and the dual fluorescence images.

The present invention discloses a dual-fluorescence anti-counterfeiting fiber of plant-based upconversion and downconversion materials, its preparation method and application. EuCl<subgt;3< / subgt; solution and TTA solution are successively added to the plant fiber solution, and then the pH is adjusted to 7 and stirred for 60 min. Then, NaYF<subgt;4< / subgt;:Yb,Er solution is added. Finally, it is washed three times with deionized water, filtered by suction, and dried to obtain the dual-fluorescence anti-counterfeiting fiber of plant-based upconversion and downconversion materials. The obtained dual-fluorescence fiber of upconversion and downconversion materials can achieve green fluorescence emission under 980 nm excitation and red fluorescence emission under 365 nm excitation. The present invention not only endows the fiber with important application value of dual-fluorescence performance in the field of anti-counterfeiting, but also has a simple preparation process, easy-to-control conditions and no pollution to the environment.

The invention discloses a fluorescence enhancement anchoring agent based on a BODIPY-tetrazine dye for an expansion microimaging technology, and belongs to the technical field of biochemical analysis. A fluorophore of the fluorescence enhanced anchoring agent is shown as a formula (I), a formula (II) or a formula (III): # imgabs 0 # imgabs 1 #; a tetrazine group carried by the fluorescence enhanced anchoring agent and a drug probe are subjected to a biological orthogonal reaction to remove fluorescence quenching; the drug probe is shown as a formula (IV): # imgabs2. The fluorescence enhancement anchoring agent can anchor covalent and non-covalent small molecules in a hydrogel network based on tetrazine biological orthogonal reaction, and meanwhile, expansion microscopic fluorescence imaging of a dual fluorescence enhancement mechanism is realized. Besides, according to the drug probe, a non-covalent drug gefitinib is selected as a probe core, a BCN click handle is modified, in-situ fluorescence expansion microscopic imaging of EGFR drug-target is successfully achieved, and a new tool is provided for non-covalent drug target co-localization information research and clinical research of exosomes.

The invention discloses a kit for detecting toxin-producing vibrio cholerae based on a dual-fluorescence RPA (recombinase polymerase amplification) technology and application of the kit. The invention provides a complete set of primers and probes. The complete set of primers and probes comprises a primer Vc-F1, a primer Vc-R4, a probe Vc-P, a primer ctxB-F, a primer ctxB-R and a probe ctxB-P, according to the invention, countless tests are matched with specific retrieval, a section of specific conserved sequence Vc and virulence gene ctxB on a pathogen genome are selected as detection targets, and whether a to-be-detected sample is toxigenic vibrio cholerae or not can be rapidly determined by simultaneously detecting the two targets. According to the invention, a brand new technical support is provided for on-site rapid diagnosis and epidemic disease investigation of vibrio cholerae by applying a dual-fluorescence RPA technology.

The present invention discloses a method for detecting silver ion content with dual fluorescence and phosphorescence responses, belonging to the technical field of heavy metal ion detection. The method includes the following steps: synthesis of polyaminosiloxane, establishment of a standard working curve for detecting silver ion concentration, and determination of actual samples. The method of the present invention determines the silver ion content by detecting the changes in the fluorescence and phosphorescence intensities of the detector, and can achieve rapid and efficient dual-response detection. This highly sensitive, rapid, and accurate detection method has strong practicability, is suitable for industrial production, and has broad application prospects in the field of heavy metal ion detection.

The present invention provides a method for preparing and applying a cold white light lignin-based carbon dot composite material with dual fluorescence / phosphorescence emission. This method uses alkali lignin as a precursor, first synthesizing lignin-derived carbon dots via a hydrothermal method, then covalently bonding them to a boric acid matrix via high-temperature calcination, resulting in a single-component cold white light lignin-based carbon dot composite material. The raw materials used in this invention are renewable, inexpensive, and readily available. The resulting white light carbon dot material exhibits a high fluorescence quantum yield and excellent stability. It exhibits cold white fluorescence emission under ultraviolet light excitation and green phosphorescence emission upon removal of ultraviolet light. It can be used in the preparation of white light-emitting diodes or for anti-counterfeiting applications.

The invention relates to the technical field of flow fluorescence, in particular to a method based on flow fluorescence and a sample analyzer. The method comprises the following steps: acquiring detection information of a sample, and detecting a microsphere compound prepared from the sample based on the detection information to obtain a light detection signal; identifying according to a two-dimensional signal distribution diagram formed by at least two optical signals to obtain effective item groups with the same number as the detection items; when the detection mode is a dual-fluorescence mode, processing the coordinates of the effective item group according to a preset weight; the effective item groups are sorted to obtain item numbers corresponding to the effective item groups, and the item numbers correspond to the detection items; and outputting a detection result of each detection item of the sample based on the effective item group and the corresponding item number. By determining the effective project groups, a data basis is provided for arrangement of the project groups; effective project groups and project numbers which are irregularly arranged correspond to each other through coordinate processing, so that the accuracy of a detection result is improved.

A multi-functional near-infrared fluorescent polymer dot (Pdot)-siRNA nanoplatform is disclosed herein. The disclosed technology addresses challenges in siRNA delivery, including poor stability, degradation, and immune recognition, by utilizing positively charged Pdots synthesized from polymers, and electrostatic binding with negatively charged siRNA. The Pdots exhibit dual fluorescence emission at 588 nm and 775 nm, enabling real-time visualization of cellular uptake and siRNA delivery. The nanoplatform demonstrates efficient inhibition of target gene expression, and protein levels in cells. The Pdots provide minimal toxicity and persist in cells for extended periods, offering a robust tool for therapeutic applications, bioimaging, and molecular labeling. This approach combines siRNA delivery with simultaneous imaging, presenting a versatile method for targeted gene regulation and research applications.

The present invention relates to a dual fluorescence probe for detecting nitroreductase and hydrogen sulfide, and a manufacturing method therefor. The dual fluorescence probe according to the present invention can detect both nitroreductase and hydrogen sulfide, which are indicators of a hypoxic environment, and can provide low background fluorescence noise and selective, high fluorescence signals, thus enabling normal cells and tumor cells in a hypoxic microenvironment to be accurately distinguished and detected through improved hypoxia detection effects and imaging capabilities.

The invention provides a dual fluorescent quantitative PCR (polymerase chain reaction) kit for simultaneously detecting streptococcus pasteurii and streptococcus suis, and belongs to the field of bacterial detection. The kit comprises a primer pair and a probe. The primer pair and the probe aiming at the streptococcus pasteurii comprise SP-3230F, SP-3230R and SP-probe, and the nucleotide sequences of the primer pair and the probe are as shown in SEQ ID NO: 1-3; the primer pair and the probe aiming at the streptococcus suis comprise SS-recNF, SS-recNR and SS-probe, and the nucleotide sequences of the primer pair and the probe are as shown in SEQ ID NO: 4-6. The kit provided by the invention has the advantages of high sensitivity, high specificity, low pollution and real-time detection, and provides reliable technology and product for early diagnosis, monitoring, early warning, prevention and treatment of clinical swine streptococcosis in farms.

The invention relates to the technical field of luminescent materials, and discloses an organic compound, an amphiphilic polymer and application thereof, and the organic compound has a structure as shown in a formula (I). The organic compound and the amphiphilic polymer provided by the invention have dual fluorescence emission, and can be used for amphiphilic aggregation-induced emission (AIE) polymer fluorescent materials. # imgabs0 #

The application provides a medical dual-fluorescence imaging method and device for fusing a white light background image, comprising: setting light emission logic of a fusion light source and irradiating an imaging area according to the light emission logic, collecting and splitting white light and first long-wavelength fluorescence, short-wavelength fluorescence and second long-wavelength fluorescence in time, collecting the white light and the short-wavelength fluorescence in time by using a first camera, collecting the first long-wavelength fluorescence and the second long-wavelength fluorescence in time by using a second camera, performing image fusion processing on white light image data, first long-wavelength fluorescence image data, short-wavelength fluorescence image data and second long-wavelength fluorescence image data, and obtaining a fused image. The application outputs the short-wavelength fluorescence and the white light in the same path and outputs the long-wavelength fluorescence separately by designing a time-sharing light emission mode combined with a prism splitting mode, achieves the effect of fusing the white light background image when dual-fluorescence simultaneous development imaging, the prism structure is relatively simple, the fluorescence signal cross-talk can be avoided, and the overall fused image is bright and clear in color.

The invention discloses a dual TaqMan real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection method for zonal Brucella wild strain infection and an A19 delta bpe275 labeled vaccine strain. The dual TaqMan real-time fluorescent quantitative PCR detection method comprises the following steps: extracting nucleic acid of a sample to be detected; the extracted nucleic acid is taken as a template, fluorescent quantitative PCR amplification is simultaneously carried out in the same reaction system, and the reaction system comprises a first primer probe combination for detecting the bpe275 gene, collecting fluorescent signals of the first fluorescent reporter group and the second fluorescent reporter group, and interpreting the detection result according to the amplification condition of the fluorescent signals. According to the present invention, the non-specific amplification risk is significantly reduced by using the specific primer and probe combination aiming at the bpe275 and bab11216 genes, the accuracy and the reliability of the detection result are ensured, the dual-target detection can be completed in the one-time reaction by using the dual-fluorescence real-time quantitative PCR technology, the detection efficiency is significantly improved, and the kit is suitable for the large-batch sample detection.

The invention provides a double qPCR method, a reagent and a kit for realizing ultra-early diagnosis of African swine fever by jointly detecting nucleic acids of host biomarkers HP and ASFV. According to the present invention, the HP and B646L genes are adopted to establish the dual fluorescence PCR method, the biomarker HP and ASFV nucleic acid detection are innovatively combined for use, the ASFV infection is diagnosed, the potential ASFV early stage infected pigs in the pig farm can be screened, the diagnosis blank period before the occurrence of the viremia after the ASFV infection is filled, and the kit can be used for the diagnosis of the ASFV early stage. Especially, a new technical tool is provided for coping with challenges of African swine fever virus variation and recombinant strains. According to the method, the efficiency of biological safety measures can be improved, the implementation precision of a'detection-killing 'strategy is optimized, the method is suitable for precise removal when ASF epidemic outbreak occurs in a pig farm or ASF epidemic outbreak occurs in the periphery, and an early monitoring and early warning means is provided for epidemic prevention and control.

According to the invention, a dual-fluorescence PCR (Polymerase Chain Reaction) detection reagent for the porcine circovirus type 2 and the porcine circovirus type 3 is prepared, and nucleotide sequences of detection primers are shown as SEQ ID NO.1-6. The detection kit provided by the invention can specifically detect the porcine circovirus type 2 and the porcine circovirus type 3, and has a remarkable application value in epidemiological monitoring of the porcine circovirus.

The invention discloses an acid stimuli-responsive metal-organic hybrid perovskite optical waveguide material and a preparation method thereof. The method specifically comprises the following steps: mixing hydrochloric acid or hydrobromic acid, 3-chloro-4-hydroxypyridine and metal halide salt, adding water, ultrasonically dissolving, heating or not heating, and separating out crystals after the solvent is volatilized, thereby obtaining the acid stimuli-responsive metal-organic hybrid perovskite optical waveguide crystal material. The material has fluorescence-phosphorescence dual emission, presents luminescence conversion of different colors under ultraviolet irradiation after being fumigated by different acids, and prolongs afterglow time after stopping light excitation. And information encryption is realized by using different luminescence colors and afterglow time after different acid fumigation materials are used. The invention provides a new thought and approach for preparing novel intelligent materials and optical information encryption devices with excellent optical characteristics.

According to the dual-emission fluorescent carbon dots synthesized through the alcohol-thermal method and the application of the dual-emission fluorescent carbon dots, 2, 4-diaminobenzene sulfonic acid and a cross-linking auxiliary agent serve as carbon sources, ethyl alcohol serves as a solvent, a carbon dot solution is prepared through a synthesis reaction, the carbon dot solution is purified to obtain powdery carbon dots, and the cross-linking auxiliary agent is polyethyleneimine, citric acid, o-phenylenediamine or acetic acid; the carbon dots have double fluorescence emission peaks. The synthesized carbon dots have the properties of good optical stability, independent excitation and the like. The dual-fluorescence emission peak of the solution system is sensitive to the pH value of the solution and a water solvent, and has the luminescent properties of up-conversion fluorescence, long afterglow in a solid-phase matrix and the like. The whole preparation process has the advantages of simplicity in operation, low cost and easiness in repetition. The fluorescence signal of the carbon dot has linear quenching response to the acetylpromazine maleate drug, and can be used for establishing a novel drug content analysis method.

The present invention provides a dual fluorescent quantitative PCR kit for simultaneously detecting Streptococcus pasteurianus and Streptococcus suis, belonging to the field of bacterial detection. The kit includes a primer pair and a probe. The primer pair and probe for Streptococcus pasteurianus include SP-3230F, SP-3230R and SP-probe, and the nucleotide sequence is shown in SEQ ID NO: 1-3; the primer pair and probe for Streptococcus suis include SS-recNF, SS-recNR and SS-probe, and the nucleotide sequence is shown in SEQ ID NO: 4-6. The kit of the present invention has the advantages of high sensitivity, high specificity, low pollution and real-time detection, and provides reliable technology and products for the early diagnosis, monitoring, early warning and prevention of clinical streptococcal disease in pigs in farms.

The present invention discloses an ultra-sensitive fluorescent small molecule probe for biothiols, a preparation method thereof, and an application thereof. The present invention proposes a dual fluorescence quenching mechanism combining the PET effect and the "ester to carboxylic acid" conversion strategy to construct an MCB-NBD probe with "zero" intrinsic fluorescence. The small molecule fluorescent probe MCB-NBD of the present invention overcomes the problems of high background fluorescence, low sensitivity, and high detection limit of existing small molecule fluorescent probes. The fluorescent small molecule probe MCB-NBD of the present invention exhibits a very low detection limit for biothiols. When 200nM biothiols are added, the fluorescence response multiple exceeds 3 times, and it can significantly distinguish between liver-damaged plasma and normal plasma at 80-fold dilution. The fluorescent small molecule probe MCB-NBD of the present invention monitors the fluctuation of biothiols in dihydroartemisinin-induced ferroptosis, reflecting the application prospect of the present invention in studying new strategies for clinical tumor treatment.

This invention proposes a quantum dot@ZIF-8 composite material, its preparation method, and its applications, belonging to the field of functional nanomaterials and fluorescence sensing analysis and detection technology. This invention involves mixing CdTe quantum dots (CdTe QDs) with red fluorescence emission and carbon quantum dots (C QDs) with blue fluorescence emission in a specific ratio, followed by in-situ self-assembly growth of ZIF-8, resulting in a quantum dot@ZIF-8 composite material with dual fluorescence signal emission. The quantum dot@ZIF-8 nanocomposite material prepared by this method not only possesses the fluorescence properties of quantum dots but also largely retains the original structure and properties of ZIF-8, making it a simple and effective method for preparing composite materials with excellent fluorescence performance. This quantum dot@ZIF-8 composite material can be used to prepare ratiometric fluorescence sensing probes for the visual detection of glucose with high sensitivity.

This invention relates to the field of molecular biology detection technology, specifically to a dual-fluorescence quantitative PCR primer and probe for detecting *Isococcus suis* and enterotoxigenic *Escherichia coli*, and their applications. The dual-fluorescence quantitative PCR primer and probe include an upstream primer ITS1-F (SEQ ID NO. 1), a downstream primer ITS1-R (SEQ ID NO. 2), and a probe ITS1-Probe (SEQ ID NO. 3) for *Isococcus suis*, and an upstream primer ST-F (SEQ ID NO. 4), a downstream primer ST-R (SEQ ID NO. 5), and a probe ST-Probe (SEQ ID NO. 6) for *Escherichia coli*. The dual-fluorescence quantitative PCR primer and probe provided by this invention have high sensitivity, high specificity, and good reproducibility, enabling simultaneous detection of *Isococcus suis* and enterotoxigenic *Escherichia coli*, thus improving diagnostic efficiency.

The invention provides a europium-based metal-organic framework ratio fluorescence detection copper ion and a green preparation process thereof, and relates to the technical field of metal-organic framework materials, the preparation method synthesizes europium-based MOF through a water phase reaction under a normal temperature condition, and the preparation method comprises the following specific steps: dissolving 2-aminoterephthalic acid and rhodamine B in a water phase, and adding an organic solvent, the preparation method comprises the following steps: mixing and stirring with a europium salt solution, and centrifugally washing to obtain a target material, the material has double fluorescence emission characteristics (435 nm and 588 nm), and compared with a traditional solvothermal method, the preparation process does not need high temperature and high pressure, is low in energy consumption and is environment-friendly; the detection method is high in anti-interference capability, the selectivity is superior to that of coexisting ions such as Fe and CrO, and the response time is only 1 minute. The technology can be widely applied to monitoring of copper ions in industrial wastewater, drinking water and biological fluid, has the advantages of high efficiency, low cost and portability, and has a remarkable industrial application prospect.

The present invention provides a dual fluorescent quantitative PCR primer-probe combination, a kit, and applications thereof for detecting snake adenovirus and lizard adenovirus. The nucleotide sequence of the primer-probe combination is shown in SEQ ID NOs: 1-6. The kit and detection method provided by the present invention have good sensitivity and strong specificity, with a minimum nucleic acid detection concentration of 80 copies / mL. These methods can meet the requirements of simple, economical, rapid, and accurate testing in clinical testing, and enable simultaneous differential detection of two pathogens in the same reaction tube. These methods have important application value in the detection and differentiation of snake adenovirus and lizard adenovirus, as well as in epidemic monitoring and epidemiological surveys.

The invention discloses a bifluorescence detection method for detecting gene expression in filamentous fungi and an exogenous gene expression method. The invention provides a double-fluorescence detection system and a double-fluorescence detection method capable of efficiently and rapidly detecting the expression efficiency of different promoters and insertion site genes of filamentous fungi, the influence caused by the growth difference of strains can be eliminated, the quantitative detection of the gene expression condition is realized, and the advantages of high efficiency and accuracy are achieved. Six efficient insertion sites are screened out, more possibilities are provided for successful heterologous expression of complex high-value natural products in aspergillus nidulans, and high application value is achieved.

An aspect of the disclosure provides a system (1) for detection of a target biomolecule (12) or a composition comprising a target biomolecule (12), the system (1) comprising: an active material (3) configured to react with the target biomolecule (12), or with the composition comprising a target biomolecule (12), to provide a dual fluorescence, wherein the dual fluorescence is excitable by illumination; an applicator (5) configured to deliver the active material (3) to an article (4) for detection of the target biomolecule (12), or of the composition comprising a target biomolecule (12); a light source (7) configured to illuminate the article (4) with wavelengths selected to excite the dual fluorescence; and, an optical detector (9, 11) configured to detect a component of the dual fluorescence.

The present application relates to a single fluorescence method and a double fluorescence method for rapid detection of Cronobacter dublinensis and Cronobacter univans by ERA technology. The present application also relates to an oligonucleotide primer probe composition for the method. The present application also relates to a detection kit comprising the composition. The ERA fluorescence method detection using the composition of the present application can simply, rapidly, specifically and sensitively detect Cronobacter dublinensis and Cronobacter univans.