7 results about "Dual fluorescence" patented technology

The application discloses an aggregation-induced emission compound with double fluorescence emission characteristics and a preparation method and application thereof, and the proportional fluorescence probe has a structure of general formula I. The probe molecule has a polar response group tetraphenyl ethylene as a fluorescence report unit, introduces a hydrophilic oxygen atom, connects an alpha carbon of an acetamide, and connects a polar detection sensitization group Tag chain on an amino group of the acetamide. The probe molecule improves the conjugation degree of oxygen atom lone pair electrons and a tetraphenyl ethylene pi electron system, so that the probe molecule simultaneously produces n→pi* and pi→pi* molecular transitions, and a proportional fluorescence probe which can sensitively respond to polarity change is constructed. The fluorescence probe designed in the application has the advantages that the fluorescence probe is not easily interfered by probe concentration, a detection environment and light bleaching, and experiments prove that the proportional fluorescence intensity of the fluorescence probe can sensitively respond to polarity, and the proportional fluorescence intensity and water content are linearly fitted, so that the purposes of detecting water content in an organic reagent and alcohol content in wine are achieved.

The invention discloses a dual TaqMan real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection method for zonal Brucella wild strain infection and an A19 delta bpe275 labeled vaccine strain. The dual TaqMan real-time fluorescent quantitative PCR detection method comprises the following steps: extracting nucleic acid of a sample to be detected; the extracted nucleic acid is taken as a template, fluorescent quantitative PCR amplification is simultaneously carried out in the same reaction system, and the reaction system comprises a first primer probe combination for detecting the bpe275 gene, collecting fluorescent signals of the first fluorescent reporter group and the second fluorescent reporter group, and interpreting the detection result according to the amplification condition of the fluorescent signals. According to the present invention, the non-specific amplification risk is significantly reduced by using the specific primer and probe combination aiming at the bpe275 and bab11216 genes, the accuracy and the reliability of the detection result are ensured, the dual-target detection can be completed in the one-time reaction by using the dual-fluorescence real-time quantitative PCR technology, the detection efficiency is significantly improved, and the kit is suitable for the large-batch sample detection.

This invention proposes a quantum dot@ZIF-8 composite material, its preparation method, and its applications, belonging to the field of functional nanomaterials and fluorescence sensing analysis and detection technology. This invention involves mixing CdTe quantum dots (CdTe QDs) with red fluorescence emission and carbon quantum dots (C QDs) with blue fluorescence emission in a specific ratio, followed by in-situ self-assembly growth of ZIF-8, resulting in a quantum dot@ZIF-8 composite material with dual fluorescence signal emission. The quantum dot@ZIF-8 nanocomposite material prepared by this method not only possesses the fluorescence properties of quantum dots but also largely retains the original structure and properties of ZIF-8, making it a simple and effective method for preparing composite materials with excellent fluorescence performance. This quantum dot@ZIF-8 composite material can be used to prepare ratiometric fluorescence sensing probes for the visual detection of glucose with high sensitivity.

This invention relates to the field of molecular biology detection technology, specifically to a dual-fluorescence quantitative PCR primer and probe for detecting *Isococcus suis* and enterotoxigenic *Escherichia coli*, and their applications. The dual-fluorescence quantitative PCR primer and probe include an upstream primer ITS1-F (SEQ ID NO. 1), a downstream primer ITS1-R (SEQ ID NO. 2), and a probe ITS1-Probe (SEQ ID NO. 3) for *Isococcus suis*, and an upstream primer ST-F (SEQ ID NO. 4), a downstream primer ST-R (SEQ ID NO. 5), and a probe ST-Probe (SEQ ID NO. 6) for *Escherichia coli*. The dual-fluorescence quantitative PCR primer and probe provided by this invention have high sensitivity, high specificity, and good reproducibility, enabling simultaneous detection of *Isococcus suis* and enterotoxigenic *Escherichia coli*, thus improving diagnostic efficiency.

An aspect of the disclosure provides a system (1) for detection of a target biomolecule (12) or a composition comprising a target biomolecule (12), the system (1) comprising: an active material (3) configured to react with the target biomolecule (12), or with the composition comprising a target biomolecule (12), to provide a dual fluorescence, wherein the dual fluorescence is excitable by illumination; an applicator (5) configured to deliver the active material (3) to an article (4) for detection of the target biomolecule (12), or of the composition comprising a target biomolecule (12); a light source (7) configured to illuminate the article (4) with wavelengths selected to excite the dual fluorescence; and, an optical detector (9, 11) configured to detect a component of the dual fluorescence.

The present application relates to a single fluorescence method and a double fluorescence method for rapid detection of Cronobacter dublinensis and Cronobacter univans by ERA technology. The present application also relates to an oligonucleotide primer probe composition for the method. The present application also relates to a detection kit comprising the composition. The ERA fluorescence method detection using the composition of the present application can simply, rapidly, specifically and sensitively detect Cronobacter dublinensis and Cronobacter univans.

The present application relates to the single fluorescence method and double fluorescence method for rapid detection of Cronobacter sakazakii and Cronobacter zurichensis by using ERA technology. The present application also relates to the oligonucleotide primer probe composition for the method. The present application also relates to the detection kit comprising the composition. The ERA fluorescence method detection using the composition of the present application can simply, rapidly, specifically and sensitively detect Cronobacter sakazakii and Cronobacter zurichensis.