8results about How to "Reliable experimental basis" patented technology

The utility model relates to experimental device technical field, concretely relates to a kind of paddy field soil remediation experimental device based on photosynthetic bacteria. Specific technical scheme is: including soil culture container, multiple partitions are horizontally and detachably arranged in the soil culture container, a plurality of small holes are arranged on the partition, filter screen is arranged on the partition and the inner bottom surface of soil culture container, a channel with one end opening is horizontally arranged in the partition, the top of the partition is provided with multiple through holes communicated with the channel, pipeline is inserted in the channel, the top surface of the pipeline is provided with multiple insertion holes, and corresponds with the through hole, diffusion tube is sequentially inserted into insertion hole and communicated by passing through filter screen and through hole, liquid inlet pipe is arranged outside soil culture container, liquid inlet pipe is communicated with pipeline, and liquid inlet pipe is communicated with bacteria liquid tank.The utility model can simulate paddy field ecological environment, provide suitable growth and metabolism conditions for photosynthetic bacteria, so as to systematically study the repair effect of photosynthetic bacteria on paddy field soil.

The invention provides a transmission electron microscope sample preparation device and method for high-entropy alloy nanoparticles, and relates to the field of high-entropy alloys and microscopic characterization thereof. The preparation device comprises a substrate and a round cover with holes, grooves of the substrate are in one-to-one correspondence with the holes in the round cover, and the device is made of a metal material with high temperature resistance. According to the preparation method provided by the invention, transmission electron microscope characterization results of the nanoscale high-entropy alloy particles and the high-quality high-entropy alloy nanoparticles can be obtained. The obtained high-entropy alloy particles are uniform in distribution, controllable in size and high in experiment success rate, and a plurality of samples can be prepared at the same time. Through the pulse laser deposition technology, the high-entropy alloy nano-particles which are evenly distributed and controllable in size can be obtained, the experiment success rate can be increased, pollution in the experiment process can be avoided, the capacity of preparing a plurality of transmission electron microscope samples at the same time is achieved, and a high-quality microscopic characterization result can be obtained; and the quality and the preparation efficiency of the high-entropy alloy transmission electron microscope nanoparticle sample are remarkably improved.

The invention discloses a method for crystallizing BM3 protein in bacillus megaterium. The method comprises the following steps: constructing a gene for coding the BM3 protein into an expression vector, carrying out induced expression in escherichia coli, carrying out affinity chromatography purification, excising a fusion tag, carrying out gel filtration purification, incubating with cofactors FAD, FMN and NADPH to form a compound, and crystallizing under a specific pool liquid condition through a seated drop gas phase diffusion method. According to the method, the high-quality BM3 protein crystal suitable for X-ray diffraction analysis can be obtained, and a basis is provided for analyzing the three-dimensional structure of the BM3 protein crystal and researching an electron transfer mechanism.

The invention belongs to the technical field of tumor animal models, and particularly relates to a construction method and application of a brain glioma PDTX model. The invention formulates a tumor screening method suitable for constructing the brain glioma PDTX model, tumor samples with relatively high GFAP, SOX2, SLC1A3 and OLIG2 gene transcription levels are screened as ideal tumor sources by performing transcriptome sequencing on tumor tissues, and the model is constructed by the novel brain glioma PDTX model construction method provided by the invention. According to the method, the screened brain glioma tissue is transplanted into the immunodeficient mouse, so that the original microenvironment characteristics of the tumor can be effectively reserved, and the success rate of constructing the brain glioma PDTX model is obviously improved.

The invention relates to the technical field of medicines, in particular to a composition containing polygonatum sibiricum saponin, a preparation method, a preparation and application in immune aging resistance. The total content of polygonatum sibiricum saponin in the polygonatum sibiricum saponin extract in the composition is 20-35%, the purity of peach gum polysaccharide is 80-95%, and the mass ratio of the polygonatum sibiricum saponin extract to the peach gum polysaccharide is (1-3): (1-2), preferably 2: 1. Animal experiments show that the composition can significantly reduce the proportion of myeloid propensity CD150hi hematopoietic stem cells, GMP, CMP and MEP in marrow of an aged mouse, improve the proportion of lymphatic propensity CD150lo hematopoietic stem cells and CLP, and correct the myeloid differentiation bias of the aged bone marrow hematopoietic stem cells; meanwhile, the proportion of bone marrow and peripheral blood myeloid cells can be reduced, and the proportion of B cells, T cells and NK cells can be increased, so that the immune aging phenotype is improved, and the method is suitable for preparing drugs or health care products for regulating differentiation of senescence bone marrow hematopoietic stem cells and resisting immune aging.

The embodiment of the invention provides a method and a device for determining the value of a control rod of a nuclear reactor. The method comprises the following steps: acquiring burnup depth and cooling time of a fuel assembly in a nuclear reactor, and source range counting rates in an initial state and a control rod group insertion state; calculating according to the burn-up depth and the cooling time to obtain an initial subcritical correction factor; calculating according to the initial subcritical correction factor and the source range counting rate in each state to obtain the measurement subcritical degree in each state; according to the measurement subcriticality, iteratively updating the initial subcriticality correction factor until an iteration stop condition is met, and taking the measurement subcriticality obtained at the last time as a target measurement subcriticality; and calculating according to the target measurement subcriticality to obtain the control rod value of the control rod group. According to the embodiment of the invention, the correction factor can be dynamically optimized in combination with the actually measured counting rate, the accuracy of the control rod value measurement result is improved, and the purpose of monitoring the difference between the design model and the actual reactor core is achieved.

The application discloses an oil-water multiphase flow monitoring and identifying system based on microfluidics and fluorescence microscopy, and relates to the field of oil-water multiphase flow monitoring and identifying systems; comprising a microfluidic chip, a fluid injection control, a microscopic imaging, an image processing module and a produced liquid collecting device. The microfluidic chip is etched based on real core CT data and accurately simulates pore structure; the fluid injection control module realizes high-precision liquid injection and constant temperature control; the microscopic imaging module captures four-phase fluid dynamics through multi-band fluorescence imaging; the image processing module carries self-developed algorithms, can identify six types of remaining oil and complete multi-dimensional quantitative analysis, and the results can feedback injection parameters. The system solves the problems of real-time observation loss, high cost and indirect mechanism inference of traditional core experiments, realizes in-situ visualization of the displacement process, data precision and intelligent regulation and control, is suitable for chemical flooding mechanism research of complex reservoirs, and provides an efficient research platform for improving recovery efficiency.

The application relates to the technical field of diagnosis and treatment of biomedical and ophthalmic diseases, and particularly relates to application of glycosphingolipid protein glycan-1 (Glypican-1, GPC1) in diagnosis and treatment of subretinal fibrosis, and the application is characterized in that GPC1 is found to be significantly changed in expression in a sample of a patient with subretinal fibrosis through proteomics screening, and it is verified through in-vivo and in-vitro experiments that GPC1 is up-regulated in expression in the process of subretinal fibrosis and plays a role in promoting fibrosis, thereby providing a new technical basis for early detection, risk assessment and targeted treatment of subretinal fibrosis.