5results about How to "Reliable experimental basis" patented technology

The invention discloses a method for crystallizing BM3 protein in bacillus megaterium. The method comprises the following steps: constructing a gene for coding the BM3 protein into an expression vector, carrying out induced expression in escherichia coli, carrying out affinity chromatography purification, excising a fusion tag, carrying out gel filtration purification, incubating with cofactors FAD, FMN and NADPH to form a compound, and crystallizing under a specific pool liquid condition through a seated drop gas phase diffusion method. According to the method, the high-quality BM3 protein crystal suitable for X-ray diffraction analysis can be obtained, and a basis is provided for analyzing the three-dimensional structure of the BM3 protein crystal and researching an electron transfer mechanism.

The invention relates to the technical field of medicines, in particular to a composition containing polygonatum sibiricum saponin, a preparation method, a preparation and application in immune aging resistance. The total content of polygonatum sibiricum saponin in the polygonatum sibiricum saponin extract in the composition is 20-35%, the purity of peach gum polysaccharide is 80-95%, and the mass ratio of the polygonatum sibiricum saponin extract to the peach gum polysaccharide is (1-3): (1-2), preferably 2: 1. Animal experiments show that the composition can significantly reduce the proportion of myeloid propensity CD150hi hematopoietic stem cells, GMP, CMP and MEP in marrow of an aged mouse, improve the proportion of lymphatic propensity CD150lo hematopoietic stem cells and CLP, and correct the myeloid differentiation bias of the aged bone marrow hematopoietic stem cells; meanwhile, the proportion of bone marrow and peripheral blood myeloid cells can be reduced, and the proportion of B cells, T cells and NK cells can be increased, so that the immune aging phenotype is improved, and the method is suitable for preparing drugs or health care products for regulating differentiation of senescence bone marrow hematopoietic stem cells and resisting immune aging.

The embodiment of the invention provides a method and a device for determining the value of a control rod of a nuclear reactor. The method comprises the following steps: acquiring burnup depth and cooling time of a fuel assembly in a nuclear reactor, and source range counting rates in an initial state and a control rod group insertion state; calculating according to the burn-up depth and the cooling time to obtain an initial subcritical correction factor; calculating according to the initial subcritical correction factor and the source range counting rate in each state to obtain the measurement subcritical degree in each state; according to the measurement subcriticality, iteratively updating the initial subcriticality correction factor until an iteration stop condition is met, and taking the measurement subcriticality obtained at the last time as a target measurement subcriticality; and calculating according to the target measurement subcriticality to obtain the control rod value of the control rod group. According to the embodiment of the invention, the correction factor can be dynamically optimized in combination with the actually measured counting rate, the accuracy of the control rod value measurement result is improved, and the purpose of monitoring the difference between the design model and the actual reactor core is achieved.

The application discloses an oil-water multiphase flow monitoring and identifying system based on microfluidics and fluorescence microscopy, and relates to the field of oil-water multiphase flow monitoring and identifying systems; comprising a microfluidic chip, a fluid injection control, a microscopic imaging, an image processing module and a produced liquid collecting device. The microfluidic chip is etched based on real core CT data and accurately simulates pore structure; the fluid injection control module realizes high-precision liquid injection and constant temperature control; the microscopic imaging module captures four-phase fluid dynamics through multi-band fluorescence imaging; the image processing module carries self-developed algorithms, can identify six types of remaining oil and complete multi-dimensional quantitative analysis, and the results can feedback injection parameters. The system solves the problems of real-time observation loss, high cost and indirect mechanism inference of traditional core experiments, realizes in-situ visualization of the displacement process, data precision and intelligent regulation and control, is suitable for chemical flooding mechanism research of complex reservoirs, and provides an efficient research platform for improving recovery efficiency.

The application relates to the technical field of diagnosis and treatment of biomedical and ophthalmic diseases, and particularly relates to application of glycosphingolipid protein glycan-1 (Glypican-1, GPC1) in diagnosis and treatment of subretinal fibrosis, and the application is characterized in that GPC1 is found to be significantly changed in expression in a sample of a patient with subretinal fibrosis through proteomics screening, and it is verified through in-vivo and in-vitro experiments that GPC1 is up-regulated in expression in the process of subretinal fibrosis and plays a role in promoting fibrosis, thereby providing a new technical basis for early detection, risk assessment and targeted treatment of subretinal fibrosis.