69results about How to "Reliable experimental basis" patented technology

The invention relates to a muddy water balance type shield simulation testing system. In the structure of the system, a model box for holding soil is fixed on a base; a refrigeration freezing device is communicated with a freezing pipe in the model box; the front part of a shield machine enters the model box through a guide sleeve on the side of the model box; a cutter disk at the front part of the shield machine is connected with the output shaft of a gearbox of a hydraulic power system through a main shaft; jacks at the left and right sides of the shield machine are connected between the model box and the gearbox; a space between a partition plate behind the cutter disk of the shield machine and the cutter disk forms a muddy water cabin; and an injection pipe of a muddy water pot, whichis connected with an air pressure boosting device, penetrates into the shield machine from the tail part of the shell of the shield machine. The system provided by the invention can simulate the tunneling process of the muddy water shield machine in a submerged tunnel construction with high water pressure and can synchronously record soil pressure, water pressure, displacement and the tunneling parameters of the shield machine, thereby finding out and analyzing the mechanism and conditions of muddy water pressure balance country rock and providing more reliable experiment basis for submerged tunnel construction and the design of a muddy water shield machine.

The invention discloses a method for measuring the deformation of a large-sized work-piece, which comprises the following steps of: firstly, placing a fixed point; secondly, sticking a deformation point; thirdly, sampling and shooting a picture; fourthly, rebuilding the information of the deformation point; fifthly, combining the coordinates; sixthly, resetting the ID of the deformation point; and seventhly, calculating the deformation quantity. By the operation steps, the invention can measure the direction and size of the three dimensional deformation of the large-size workpiece, which is caused by the applied force load, thermal load, etc. The method has the characteristics of easy operation, no restriction of the size of the workpiece, abundant measurement information, precise and reliable results, high efficiency and non-contact measurement.

The invention discloses an experimental device for a flow field display. An air inlet and an air outlet are arranged at two ends of a pipeline respectively; a trace gas generating device is arranged in the pipeline and is close to the air inlet; an axial-flow fan is arranged in the pipeline and is close to the air outlet; an observation section is arranged on the pipeline with the trace gas generating device and the axial-flow fan; a model control device is arranged in the observation section; a laser sheet cutting flow field device and an image data acquisition control device are arranged above the observation section; a steady flow tube bundle is arranged in the pipeline between the trace gas generating device and the observation section; a computer is connected with the model control device, the image data acquisition control device and the axial-flow fan through data transmission lines; and a fan frequency conversion control device is connected between the computer and the axial-flow fan. The experimental device is simple and convenient in control, acquires reliable experimental data and is used for qualitative experimental research for a flow field.

The invention relates to an enzymatically decomposed clam oligopeptide having a recovery effect on a non-alcoholic fatty liver disease (NAFLD) cell model. The clam oligopeptide is characterized by comprising an amino acid sequence of Gln Leu Asn Trp Asp. The invention also relates to a preparation method of the enzymatically decomposed clam oligopeptide having the recovery effect on the NAFLD cell model. Compared with the prior art, the following advantages can be achieved: the NAFLD cell model is established by virtue of induction of palmitic acid and the damaged liver cells in a body are simulated sufficiently, and the result indicates that the TG content of the in-vitro NAFLD cell model established 48 hours after induction by 15 micrograms/milliliter palmitic acid increased remarkably in comparison with that of normal liver cells; oil red O staining shows that the number of intracellular lipid droplets of the model group is increased in contrast with that of the cells of a normal group, and the cell mortality rate is low and the repeatability is good, and therefore, the modeling method is simple, convenient and feasible; meanwhile, the enzymatically decomposed clam oligopeptide having the obvious recovery effect on the NAFLD cell model can be separated out from clams.

The invention provides a test device and a test method of critical current of superconductive wires under mixed stress. According to the device, electromagnet housings are fixed on left and right sides of a Dewar flask; an excitation coil is installed in an inner cavity of each electromagnet housing; an output shaft housing is installed on the top plate on the Dewar flask and penetrates through the top plate through a bearing; the main shaft of the motor is connected with a main gear; the main gear is engaged with a toothed ring fixed on the output shaft housing; a vertical servo motor is fixed on the top end of the output shaft housing; the output shaft of the vertical servo motor downwards penetrates through the output shaft housing and then is connected with an upper clamp; the upper clamp clamps the upper end of a superconductive wire; the lower end of superconductive wire is clamped by a lower clamp; the lower clamp is fixed on a fixed base at the bottom of the Dewar flask; a transverse servo motor is installed on a table top of a support table and is connected with a poly(tetrafluoroethylene) disc through a guidance rod and a force transmission rod; and the disc is arranged on the left side of the middle part of the superconductive wire. The test device is capable of testing the critical current of the superconductive wire under different bending radiuses, tension force, torsion angles and magnetic fields.

The invention discloses a primer, a probe, a kit and a usage method of the kit. A primer, a probe and a kit for detecting a genotype of HPV (Human Papillomavirus) and a usage method of the kit can beused for simultaneously detecting multiple HPV subgenotypes; through using a real-time fluorescence PCR (Polymerase Chain Reaction) amplification technique, the nucleic-acid extraction only needs to be carried out once; a PCR detection reaction also needs to be carried out once in each group; the usage method of the kit is quick, simple and convenient; moreover, the specificity is good; other HPVsubgenotypes or other microbes and the like cannot be detected out; therefore, a false positive problem difficultly appears; moreover, the sensitivity is high, and can reach 400copies/ml; a detectionrange is 400copies/ml to 4.00E+09copies/ml, and a detection range is wide.

The invention discloses a primer, a probe and a kit for detecting the microorganism in a urogenital canal and a use method of the kit. By the primer, the probe and the kit for detecting the microorganism in the urogenital canal and the use method of the kit, the chlamydia trachomatis, the ureaplasma urealyticum and the gonococcus can be detected simultaneously, the extraction of the nucleic acid is only conducted and only one step of the PCR detection reaction is conducted through the real-time fluorescence PCR amplification technique, the use method of the kit is simple and rapid, the specificity is good, the pathogene microorganisms of the chlamydia trachomatis, the ureaplasma urealyticum and the gonococcus are not detected, the problem of false positive result is not easy to cause, thesensitivity is as high as 400 copies/ml, the detection range is 400 copies/ml to 4.00E+09 copies/ml, and the detection range is wide.

The invention relates to a method for determining a rib laying manner of a reinforced retaining wall based on a sand box model test. Initial rib laying parameters of a wallplate are calculated out by establishing a sand box model of a simple interface model and through the Rankine earth pressure theory and the mechanism of action of a reinforced retaining wall, thus a wallplate with pull ribs is manufactured; an earth pressure model loading test is carried out, the number of arrangement layers of the pull ribs is gradually reduced, the rib laying parameters are calculated again, the earth pressure model loading test is repeated, and the optimized number of layers of the pull ribs, the horizontal intervals of all the layers and the vertical positions of all the layers are obtained; the length of the pull ribs is gradually reduced, and the earth pressure loading test is repeated until the optimized length of the pull ribs is obtained. According to the method for determining the rib laying manner of the reinforced retaining wall based on the sand box model test, the theory computing method and the earth pressure model test are effectively combined, the optimal pull rib arrangement manner of the reinforced retaining wall can be obtained more simply, more rapidly and more reliably. Thus, the reinforced retaining wall can stably and effectively play a role in supporting, the total number of layers of rib adding is minimal, the length of the pull ribs is the shortest, the consumption amount of rib materials is small and the structure is economic.

The invention discloses a device and method for a wheeltrack friction fatigue test. The device comprises a machine base and a direct current motor installed on the machine base. An output shaft of the direct current motor is connected with a large wheel of a simulated steel rail, a hydraulic cylinder is mounted on a crossbeam of the machine base, the end of a piston rod of the hydraulic cylinder extending out downwards is connected with a small wheel mounting base, and a small wheel for simulating a high-speed wheel is installed on the small wheel mounting base through a bearing. A magnetic powder brake is installed on the small wheel mounting base, one end of the a shaft of the small wheel penetrates the bearing and then is connected with an output shaft of the magnetic powder brake, a rim of the small wheel is in an arc shape in the width direction, and a rim of the large wheel is straight in the width direction. Experimental working conditions and mechanisms of the device are closer to actual working conditions of a high-speed wheeltrack and an abrasion-fatigue damage mechanism, a more accurate and more reliable experimental basis is provided for design, manufacture and maintenance of anti-abrasion-fatigue of the high-speed wheel, and thus high-speed train operation safety and economy are improved.

A method for configuring the eucaryotic expression carrier of cell factor IL-24 includes designing the ascending and descending primers, PCR amplifying begining from the second amino acid in the code at human IL-24 N terminal by using TRAPIL-24 plasmid as template, cutting off target band, purifying it with reagent kit, configuring and determining recombinant plasmid pEGFP-CI-IL-24, respectively enzyme-severing the PCR product and carrier pEGFP by Bgl II and Sal I, purifying them by reagent kit, linking fragment, culturing, cloning, determining and sequencing. The recombinant cell factor Il-24 can be used to treat liver cancer, small intestine cancer and melanoma.

The invention discloses a kit for detecting a breast cancer marker. The kit comprises the following components: magnetic bead processing solution, a magnetic bead with a breast cancer antibody, 4.0 to 5.0mu l of 10X buffer solution, 4.0 to 5.0mu l of deoxynucleoside triphosphates (dNTPs), 2.0 to 3.0mu l of reverse transcriptase, 0.4 to 0.6mu l of 40u/mu l ribonucleic acid (RNA) enzyme inhibitor, 20.0 to 30.0mu l of 10X hot start polymerase chain reaction (PCR) mixed solution, 10.0 to 15.0mu l of ddH2O and 3.0 to 5.0mu l of breast cancer marker primer. Compared with the prior art, the kit can more accurately detect the breast cancer marker in peripheral blood, and has great guiding significance for selecting breast cancer prognosis schemes and treatment schemes.

The invention discloses an RRLC-Q-TOF-MS method for detecting diversified chemical components in leaves of tetrastigma hemsleyanum diels et gilg. The RRLC-Q-TOF-MS method includes steps of a, preparing reference solution; b, detecting the reference solution; c, preparing test solution; d, detecting the test solution. The RRLC-Q-TOF-MS method has the advantages that main flavone components in the leaves of the tetrastigma hemsleyanum diels et gilg can be quickly qualitatively identified or quantitatively detected by the aid of the RRLC-Q-TOF-MS method, and accordingly reliable experiment bases can be provided for developing and utilizing the leaves of the tetrastigma hemsleyanum diels et gilg and evaluating the quality of the leaves of the tetrastigma hemsleyanum diels et gilg.

The invention discloses a method for measuring lubricating grease drop point. The method comprises the following steps of: for the lubricating grease at normal temperature, applying a periodic shearing action at certain frequency, gradually increasing the amplitude of a stress or strain; according to a dynamic mechanical behavior shown by the lubricating grease, determining a linear viscoelastic range in which the lubricating grease has basically constant energy storage modulus and loss modulus; and selecting a certain stress value or strain value in the linear viscoelastic range, maintaining the same frequency, heating the lubricating grease by a certain program, measuring the temperature at which energy storage modulus of the lubricating grease is reduced to be equal to the loss modulus, and using the measured value as the drop point of the lubricating grease. The measurement method of the invention has the advantages of effectively avoiding the interference of the size and mounting method of the instrument, the heating temperature, gravity of lubricating grease drops and the like existing in the conventional method for measuring the lubricating grease drop point, obtaining data result with higher repeatability and providing a more accurate and reliable experimental basis for the determination of the maximum service temperature of the lubricating grease or the selection of the lubricating grease under specific conditions.

The present invention belongs to the technical field of medicine, and relates to new medicinal uses of thymopeptide alpha1, specifically to uses of thymopeptide alpha1 in pulmonary fibrosis drug preparation. Based on disadvantages of extremely poor prognosis, no effective treatment method, extremely high death rate and the like in idiopathic pulmonary fibrosis treatment by using the existing drugs, a strategy for targeted Th1 type reaction increase or Th2 type reaction inhibition is adopted, thymopeptide alpha1 is adopted as an immune modulator to increase the Th1 type immune reaction and other immune modulation effects, and animal experiment results show that immune disbalance of the pulmonary fibrosis body can be corrected with the thymopeptide alpha1, infection incidence rate can be significantly reduced, and death rate can be reduced, such that the thymopeptide alpha1 can further be used for preparation of pulmonary fibrosis drugs. According to the present invention, under the status of lack of effective pulmonary fibrosis disease treatment drugs, important clinical values are provided, and reliable experimental basis is provided for wide clinical applications.

The invention provides a quick processing method suitable for large eccentricity ratio orbit dense ephemeris precision calculation. Aiming at large eccentricity ratio orbit calculation, the inventionprovides a new method for constructing interpolation base points through time transformation, the constructed non-uniform interpolation base points are matched with interpolation polynomial interpolation to obtain dense ephemeris, and the technical scheme can remarkably improve the calculation efficiency and precision. Based on a large-scale numerical experiment, the optimal universal numerical value of time conversion parameters corresponding to different orbit eccentricity ratios and various interpolation polynomials is 0.3. Besides, under the condition that the optimal pervasive numerical value is taken as the time transformation parameter, the Hermite interpolation polynomial is determined by comparing the calculation efficiency of various interpolation polynomials, and the method is agood method.

The invention proposes a method for heat-resistant analysis on hybrid xylanase AEx11A from 11 family of Aspergillus oryzae. According to the invention, a disulfide bond (cys5-cys32) is introduced at the N end of AEx11A by homologous modeling of an AEx11A structure and comparison between the AEx11A structure and an AoXyn11A structure; 5-site cysteine is subjected to mutation to form threonine (C5T) by a site-directed mutagenesis method, and the disulfide bond is removed to discuss the effect of the disulfide bond on the thermal stability of the AEx11A. The result shows that Topt of the mutant enzyme (AEx11AC5T) is reduced to 60 DEG C from 75 DEG C before mutation, and t1/270 and t1/280 are respectively shortened to 3.0 minutes and 1.0 minute from 197 minutes and 25 minutes before mutation, thus the N-end disulfide bond has a certain influence on the 11 family of xylanase, and a theoretical basis can be established for clarification of a thermal stability mechanism of the 11 family of xylanase and transformation of heat-stable protein engineering.

The invention provides a device for simulating non-homogeneity of a rock by using a checkerboard simulation body and a manufacturing method. The invention mainly aims at achieving accurate simulationof the non-homogeneity of a rock plane in a laboratory. The device is characterized by comprising an ISCO constant pressure pump, an electronic pressure meter, a five-way valve, a guide pipe, different permeability units of 2*2*10 cm, a pouring heterogeneous simulation body and a measuring cylinder. The manufacture of the device depends on the permeability distribution of the actual rock. The manufacturing method comprises the steps that firstly, the permeability data of all layers of all wells in a to-be-simulated area are collected, and then the permeability data are subjected to dispersionthrough a variation function, so that the permeability value of any point on the plane is obtained; in this way, the plane non-homogeneity of the rock can be fully reflected in the simulation body, sothat simulation of the non-homogeneity of the rock plane in the laboratory is simulated, and the influence of the non-homogeneity of the plane on the development of the oil reservoir can be more conveniently researched.

The invention discloses a method for in-situ study of an amorphization mechanism of a germanium-antimony-tellurium material under electron beam irradiation. The method comprises the following steps: selecting a germanium-antimony-tellurium crystal thin film of which the surface is deposited on a carbon supporting film copper carrying net with a standard size of a transmission electron microscope;carrying out surface pretreatment on the prepared film sample by adopting a plasma cleaning technology, and carrying out plasma cleaning by selecting argon; placing a pretreated electron microscope sample in the transmission electron microscope, and searching an area which is smooth in surface, free of carbon deposition, pollution and damage and uniform in thickness in the thin film sample to serve as an irradiation area; setting irradiation parameters, and inducing the material to be gradually amorphized; and observing and recording the evolution process of the amorphous structure of the material in situ, and analyzing the amorphous phase change mechanism of the material. According to the method, the gradual amorphization of the germanium-antimony-tellurium material is realized through electron beam irradiation, and the structural evolution process of the material amorphization is directly observed and analyzed by virtue of the transmission electron microscope, so that the convenientresearch method and a reliable experimental basis are provided for exploring the amorphization mechanism of the germanium-antimony-tellurium material.

The invention discloses a universal nucleic acid detection kit for human adenoviruses. The kit comprises an extracting solution for extracting human adenovirus DNA and a PCR reaction solution, and thereaction solution comprises 50 [mu] L of 13 [mu] M Taq DNA polymerase, 100 [mu] L of 150 [mu] M dNTP, 200 [mu] L of SyBr dye, and 50 [mu] L of 15 [mu] M uracil glycosylase. A human adenovirus positive reference substance and a human adenovirus negative reference substance; wherein the PCR reaction system is 20 [mu] L and comprises 15 [mu] L of a PCR reaction solution, 2 [mu] L of a primer and 3 [mu] L of sample DNA, and the sequence of the primer for DNA amplification reaction in the PCR reaction solution is as follows: P1: 5 '-ACATGAGGATGCTGAGTACC-3'; and P2: 3 '-CACCCCGCTGTTGTCTCTCTACCTACACCGG-5'. The universal nucleic acid detection kit and method for the human adenoviruses have very good specificity, can detect the human adenoviruses, are mature, quick and accurate in detection method, and provide reliable experimental basis for detection of the human adenoviruses.

The invention discloses a method for nondestructive measurement of active and passive constraint forces of an FRP confined concrete column based on ultrasonic waves. The method is based on an ultrasonic stress test technology. The method comprises: pre-embedding an ultrasonic transmitting sensor in the center of a cross section needing to be measured in the column to transmit ultrasonic waves; arranging an ultrasonic receiving device on the outer surface of the FRP of the same cross section to receive the test signal, and measuring the active and passive constraining forces of the FRP confinedconcrete column under pressure according to the relationship between the received acoustic-stress sensitivity parameter and the hoop constraining force. The method is simple to operate, visual in measurement result, free of damage to components and high in sensitivity, can realize real-time measurement of the active constraint force and the passive constraint force in the whole stress process ofthe FRP confined concrete column, realizes real-time detection of the hoop constraint force in the construction or service process, and can realize real-time monitoring of the safety and health of thestructure.