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RRLC-Q-TOF-MS method for detecting diversified chemical components in leaves of tetrastigma hemsleyanum diels et gilg

A RRLC-Q-TOF-MS, chemical composition technology, applied in the field of RRLC-Q-TOF-MS to detect various chemical compositions in cloverleaf, can solve the problem of less quality evaluation research and so on

Inactive Publication Date: 2017-03-22
FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] It has been reported that the quality evaluation of the root tuber of Clover is reported, but there are few studies on the quality evaluation of the leaf, another important medicinal part of Clover, and Clover is also widely used in a variety of Chinese patent medicines. And health food, such as Jieshikang capsules, Paishi Lidan capsules, three-leaf green tea, etc.

Method used

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  • RRLC-Q-TOF-MS method for detecting diversified chemical components in leaves of tetrastigma hemsleyanum diels et gilg
  • RRLC-Q-TOF-MS method for detecting diversified chemical components in leaves of tetrastigma hemsleyanum diels et gilg
  • RRLC-Q-TOF-MS method for detecting diversified chemical components in leaves of tetrastigma hemsleyanum diels et gilg

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] 1 Instruments and reagents

[0042] 1290 type ultra-high performance liquid chromatography (Agilent, USA); MicrOTOF time-of-flight mass spectrometer (Bruker, Germany); CPA225D 1 / 100,000 analytical balance (Sartorius, Germany); KQ-500E desktop ultrasonic cleaner (Kunshan City) Ultrasonic Instrument Co., Ltd.); DFY-500 type 500 grams of swinging high-speed traditional Chinese medicine pulverizer (Wenling City Linda Machinery Co., Ltd.); methanol and acetonitrile are chromatographically pure (MERCK, Germany); Milli-Q ultrapure water (Millipore, USA) ); the rest of the reagents were analytically pure.

[0043] The reference substance vitexin (batch number: 111687-200602) was purchased from China National Institutes for Food and Drug Control; other reference substances neochlorogenic acid (batch number: SH906332), cryptochlorogenic acid (batch number: SH905997), chlorogenic acid (batch number: ) was purchased from Beijing Saibaicao Technology Co., Ltd.; vitexin rhamnoside, ...

Embodiment 2

[0059] Embodiment 2 process screening of the present invention

[0060] 1. Screening of chromatographic conditions

[0061] (1) Screening of chromatographic columns

[0062] Different chromatographic columns were compared, Waters ACQUITY UPLC BEH C18 1.7μm 2.1x100mm(1), Waters ACQUITY UPLC HSS T3C18 1.8μm 2.1x100mm(2), Waters CORTECS C18 1.6μm 2.1x100mm(3), Phenomenex Kinetex C18 1.7μm( ). Other chromatographic conditions are as follows:

[0063] Mobile phase: acetonitrile (A)-0.1% formic acid (B) gradient elution, 0-0.5min, 5A%-5%A; 0.5-1.5min, 5%A-9%A; 1.5-8.5min, 9% A-12%A; 8.5-10min, 12%A-20%A; 10-13min 20%A-70%A.

[0064] The result is as image 3 As shown, Waters CORTECS C18 1.6μm 2.1x100mm has more peak capacity and better resolution, so Waters CORTECS C18 1.6μm 2.1x100mm chromatographic column is preferred.

[0065] (2) Screening of mobile phase

[0066] Four mobile phase systems were compared: acetonitrile-0.1% formic acid in water (5), methanol-0.1% formic aci...

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Abstract

The invention discloses an RRLC-Q-TOF-MS method for detecting diversified chemical components in leaves of tetrastigma hemsleyanum diels et gilg. The RRLC-Q-TOF-MS method includes steps of a, preparing reference solution; b, detecting the reference solution; c, preparing test solution; d, detecting the test solution. The RRLC-Q-TOF-MS method has the advantages that main flavone components in the leaves of the tetrastigma hemsleyanum diels et gilg can be quickly qualitatively identified or quantitatively detected by the aid of the RRLC-Q-TOF-MS method, and accordingly reliable experiment bases can be provided for developing and utilizing the leaves of the tetrastigma hemsleyanum diels et gilg and evaluating the quality of the leaves of the tetrastigma hemsleyanum diels et gilg.

Description

technical field [0001] The invention relates to a method for detecting the quality of medicinal materials, in particular to an RRLC-Q-TOF-MS method for detecting various chemical components in A. trifoliate. Background technique [0002] Clover is a rare medicinal plant unique to my country. It is a plant of the genus Tetrastigma hemsleyanum Diels et Gilg in the grape family. The main active ingredients are organic acids, flavonoids and volatile oils. [0003] It has been reported that the quality evaluation of the root tuber of Clover is reported, but there are few studies on the quality evaluation of the leaf, another important medicinal part of Clover, and Clover is also widely used in a variety of Chinese patent medicines. And health food, such as Jieshikang capsules, Paishi Lidan capsules, three-leaf green tea, etc. Contents of the invention [0004] In order to solve the above problems, the present invention provides a kind of RRLC-Q-TOF-MS method for detecting mult...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 范世明许文
Owner FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
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