123 results about "Tetrastigma hemsleyanum" patented technology

The invention belongs to the technical field of plant planting, and relates to a method for planting tetrastigma hemsleyanum dielset gilg. The method comprises the following steps of: cultivating in pots or fields; adding hard materials into pot soil or directly adding hard materials into field soil; and setting up a climbing pergola and a shade for planting tetrastigma hemsleyanum dielset gilg. Due to the adoption of the planting method, the yield of underground tubers of tetrastigma hemsleyanum dielset gilg is increased greatly, high quality utilization of the stems and leaves of tetrastigma hemsleyanum dielset gilg is enhanced greatly, and the planting risk is lowered. The method is easy and convenient on the aspect of technology, and is easy to popularize.

The invention relates to a bag type cultivation method for bionic interplanting of Tetrastigma hemsleyanum Diels et Gilg on a bamboo forest land. The bag type cultivation method comprises the steps of site selection, making of cultivation bags special for the Tetrastigma hemsleyanum Diels et Gilg, making of a cultivation substrate special for the Tetrastigma hemsleyanum Diels et Gilg, bagging of the cultivation substrate, planting of Tetrastigma hemsleyanum Diels et Gilg seedlings, arrangement of the cultivation bags, cultivation management, harvesting and the like. According to the method, the specially-made cultivation bags and cultivation substrate special for the Tetrastigma hemsleyanum Diels et Gilg are adopted for realizing wild bionic planting of the Tetrastigma hemsleyanum Diels et Gilg on the bamboo forest land in order to obtain Tetrastigma hemsleyanum Diels et Gilg medicinal root tubers. According to the method disclosed by the invention, the Tetrastigma hemsleyanum Diels et Gilg is interplanted under a natural climate condition by using the specific site condition of the bamboo forest land, so that the Tetrastigma hemsleyanum Diels et Gilg medicinal root tubers which have high quality and high yield and are comparable to wild Tetrastigma hemsleyanum Diels et Gilg medicinal root tubers in quality can be planted. Bag type cultivation is adopted, and the cultivation bags are arranged on the surface of the bamboo forest land without coming into conflict with the growth of a bamboo forest with developed root systems, so that the construction of a greenhouse and shading are not required, and the method is low in cost and easy in operation. Moreover, crop rotation can be realized, and the multi-cropping index and economic benefit of the bamboo forest land are increased greatly.

The invention belongs to the technical field of tea production, and particularly relates to a Tetrastigma hemsleyanum Duels et Gilg tea with high contents of Tetrastigma hemsleyanum Duels et Gilg flavone, polysaccharide and kaempferol, and a manufacturing method of the Tetrastigma hemsleyanum Duels et Gilg tea. The method comprises the following steps: (1), spreading fresh Tetrastigma hemsleyanumDuels et Gilg leaves for 10-20 minutes; (2), fixation: deactivating enzymes of the Tetrastigma hemsleyanum Duels et Gilg leaves by adopting a microwave tea fixation drier; (3), flat pressing for shaping: uniformly spreading the Tetrastigma hemsleyanum Duels et Gilg leaves subjected to fixation on a bamboo skin board, and pressing another bamboo skin board on the leaves to enable the cooled Tetrastigma hemsleyanum Duels et Gilg leaves to be shaped; and (4), drying: drying the semi-finished products processed in the step (2) by a microwave tea machine. The Tetrastigma hemsleyanum Duels et Gilg tea is natural green, is bright green in color and light green in grounds, does not contain caffeine, is rich in Tetrastigma hemsleyanum Duels et Gilg flavone, polysaccharide, kaempferol and other ingratiates, can effectively inhibit cancer cell proliferation, promotes cancer cell poptosis, has no toxic or side effects and is easy to absorb and beneficial to health. The Tetrastigma hemsleyanum Duels et Gilg leaves are tender, have less fibers, and can be eaten after being brewed.

The invention discloses a method for efficiently extracting flavonoid from tetrastigma hemsleyanum. The method comprises the steps of: air drying tetrastigma hemsleyanum, mincing, adding water, extracting from an ultrasonic extractor, concentrating the extractive solution to a certain volume, decolorizing with activated carbon, performing liquid extraction with ethyl acetate by using a cavitation suspension extraction and separation device, recovering the ethyl acetate, performing liquid extraction of the aqueous fraction with chloroform, recovering the chloroform, collecting the aqueous fraction, concentrating, purifying with macroporous adsorption resin, eluting with 50% water and ethanol in sequence, collecting the eluent with 50% of ethanol, recovering the solvent, and drying to obtain tetrastigma hemsleyanum extract.

The invention provides a method for authenticating medicinal tetrastigma hemsleyanum Diels et Gilg. According to the method for authenticating the medicinal tetrastigma hemsleyanum Diels et Gilg, the medicinal tetrastigma hemsleyanum Diels et Gilg is authenticated through the specificity nucleotide sequence of the tetrastigma hemsleyanum Diels et Gilg, specific primers based on the sequence of the tetrastigma hemsleyanum Diels et Gilg and the PCR technology. The primers designed through the method have high specificity, can increase by about 1800bp strips in tetrastigma hemsleyanum Diels et Gilg with twelve provenances, and cannot react with the DNA of sixteen affinis species and tetrastigma hemsleyanum Diels et Gilg fake product circular soil, and therefore the tetrastigma hemsleyanum Diels et Gilg can be fast and accurately authenticated. The method for authenticating the medicinal tetrastigma hemsleyanum Diels et Gilg can achieve the whole authentication operation with a small number of samples, the authentication result is accurate, and sensitivity is high. The method for authenticating the medicinal tetrastigma hemsleyanum Diels et Gilg is objective, fast and accurate in authentication for the medicinal tetrastigma hemsleyanum Diels et Gilg, and solves the problem that consumers are deceived by counterfeit tetrastigma hemsleyanum Diels et Gilg in the medicine market.

The invention discloses a method for efficiently and rapidly propagating a tetrastigma hemsleyanum Diels et Gilg test-tube plantlet. According to the method, an annual semi-lignified stem section of tetrastigma hemsleyanum Diels et Gilg is sterilized, rinsed and sheared, and small sections with 1-2 axillary buds are obtained and inoculated to a solid medium to be cultured; after the axillary buds germinate, the axillary buds are inoculated to a proliferation medium to be cultured and grafted once every 50-60 days until the required seedling quantity is reached, the axillary buds are inoculated to a rooting medium for rooting culture, and after 50-60 days, when the seedlings of the tetrastigma hemsleyanum Diels et Gilg grow 2-3 roots, acclimatizing and transplanting can be performed. Compared with the prior art, the method can be directly used for industrial and large-scale seedling production and has the advantages of little pollution, high proliferation speed, low aberration rate, orderly and robust seedlings, low production cost, convenience in transportation, high survival rate of transplanting and the like.

The invention discloses a planting method for tetrastigma hemsleyanum Diels et Gilg. The planting method mainly comprises the following steps of seedling selection, seedling culture, field management, pest and disease control, timely harvesting and the like. Rattan seedlings need to be treated through rooting hormone before seedling culture and insertion implantation, so the survival rate of the rattan seedlings can reach 90% or more. By the adoption of the method, the tetrastigma hemsleyanum Diels et Gilg can be produced on a large scale, so rich tetrastigma hemsleyanum Diels et Gilg herbs can be provided for people, and the medicine consumption requirements of people are satisfied. Besides, the planting method has good economic and social benefits.

The invention discloses a method for efficiently and visually discriminating the authenticity of Tetrastigma hemsleyanum Diels et Gilg by using DNA mimic enzyme. The method comprises the following steps: 1, obtaining the authenticity difference sequence of Tetrastigma hemsleyanum Diels et Gilg, and obtaining an object-to-be-detected PCR product; and 2, carrying out strand displacement cycle signal amplification technology colorimetric visualization on the object-to-be-detected PCR product to discriminate the authenticity of Tetrastigma hemsleyanum Diels et Gilg: carrying out an enzyme digestion strand displacement cycle reaction to generate a large amount of G-rich sequences for forming G-quadruplexes in a system, and forming a G-quadruplex-hemin DNA mimic enzyme in the presence of K<+> and Hemin in order to realize visual discrimination of the authenticity of Tetrastigma hemsleyanum Diels et Gilg, wherein the enzyme has horseradish peroxidase-like activity and can catalyze color development of an ABTS<2->-H2O2 system. The method has the advantages of low cost, simple DNA cultivation technology, good reappearance and good stability, avoids gel electrophoresis analysis to save a lot of time and realize direct use of the method in rapid and visual discrimination of the authenticity of Tetrastigma hemsleyanum Diels et Gilg, and is hopeful to be widely applied in the medicinal material market and the food safety field.

The invention relates to the field of agriculture, in particular to a high-yield cultivation method of Tetrastigma hemsleyanum Diels et Gilg. Through planting of Nepeta Cataria, Artemisia dracunculusand Ligusticum wallichii in spacings of seedlings of Tetrastigma hemsleyanum Diels et Gilg in a dispersing and quantitative mode, the effect of pest expelling, promotion of the growth and developmentof Tetrastigma hemsleyanum Diels et Gilg and improvement of the content of beneficial components in tuberous roots of Tetrastigma hemsleyanum Diels et Gilg are achieved; and through application of green manure which is composed of selenium-enriched alfalfa, selenium-enriched Astragalus sinicus and Polygonum hydropiper and is rich in nutrients and high in selenium content, full promotion of the growth and development of Tetrastigma hemsleyanum Diels et Gilg, improvement of the content of the beneficial components in Tetrastigma hemsleyanum Diels et Gilg and a certain degree of killing effects on pests in soil are achieved. Through the high-yield cultivation method of Tetrastigma hemsleyanum Diels et Gilg, the yield of Tetrastigma hemsleyanum Diels et Gilg and the quality of the obtained Tetrastigma hemsleyanum Diels et Gilg can be improved.

The invention discloses a bamboo forest land cultivation method imitating wild for tetrastigma hemsleyanum. The method comprises the following steps: site selection, production of a special bag of thetetrastigma hemsleyanum, production of a special cultivation substrate of the tetrastigma hemsleyanum, planting of seed seedlings of the tetrastigma hemsleyanum, cultivation management, harvesting and crop rotation. According to the cultivation method provided by the invention, the special bag of the tetrastigma hemsleyanum is formed by suturing linen, is friendly to the environment and does notneed to be punched, so that the method is simpler, and the costs are lower; and a certain ratio of bamboo leaves are added, so that soil is not easy to agglomerate during planting, the bamboo leaves has convenient sources, and the planted tetrastigma hemsleyanum has a high yield.

The invention discloses an RRLC-Q-TOF-MS method for detecting diversified chemical components in leaves of tetrastigma hemsleyanum diels et gilg. The RRLC-Q-TOF-MS method includes steps of a, preparing reference solution; b, detecting the reference solution; c, preparing test solution; d, detecting the test solution. The RRLC-Q-TOF-MS method has the advantages that main flavone components in the leaves of the tetrastigma hemsleyanum diels et gilg can be quickly qualitatively identified or quantitatively detected by the aid of the RRLC-Q-TOF-MS method, and accordingly reliable experiment bases can be provided for developing and utilizing the leaves of the tetrastigma hemsleyanum diels et gilg and evaluating the quality of the leaves of the tetrastigma hemsleyanum diels et gilg.

The invention discloses an ointment for treating rhagadia and a preparation method of the ointment. The ointment comprises the following raw materials in part by weight: 8 to 10 parts of Chinese angelica, 3 to 4 parts of pseudo-ginsheng, 4 to 6 parts of glycyrrhiza, 5 to 7 parts of cortex dictamni, 6 to 9 parts of fructus kochiae, 5 to 7 parts of Chinese parsnip root, 9 to 12 parts of tribulus terrestris, 2 to 3 parts of ginseng, 5 to 7 parts of tetrastigma hemsleyanum, 4 to 6 parts of radix isatidis, 3 to 4 parts of laciniate kalanchoe herb, 4 to 6 parts of pericarpium papaveris, 8 to 12 parts of dried alum, 4 to 5 parts of ampelopsis japonica and 15 to 20 parts of beewax. The ointment can treat hand and foot rhagadia fundamentally, and has obvious effects; the raw materials are lower in cost, and have no side effect; the rhagadia is not easy to relapse.

The invention discloses a composition obtained by fermenting tetrastigma hemsleyanum diels et gilg. through edible and medicinal fungi and a preparation method of the composition. The composition comprises a tetrastigma hemsleyanum diels et gilg. fermentation subcritical extract I and a streptomycete fermentation product III, and has a favorable function of reducing blood lipid. The preparation method comprises the following steps of inoculating Brazilian mushroom liquid seed fluid into tetrastigma hemsleyanum diels et gilg. solid state fermentation culture mediums for culturing, wherein the tetrastigma hemsleyanum diels et gilg. solid state fermentation culture mediums contain tetrastigma hemsleyanum diels et gilg. and bletilla striata, and then placing the cultured Brazilian mushroom liquid seed fluid in alternating magnetic field environment for culturing once again so as to obtain tetrastigma hemsleyanum diels et gilg. solid state fermentation products; then performing subcritical water extraction so as to obtain the tetrastigma hemsleyanum diels et gilg. fermentation subcritical extract I; inoculating streptomycete seed fluid into streptomycete liquid fermentation culture mediums for culturing so as to obtain the streptomycete fermentation products III, wherein the streptomycete liquid fermentation culture mediums contain extraction remnants II. According to the method disclosed by the invention, nutrient components and effective components in the tetrastigma hemsleyanum diels et gilg. can be effectively biodegraded and utilized, and the increase of the content and the activity of active components in finished products is facilitated.

The invention discloses passion fruit healthcare beverage. The passion fruit healthcare beverage is characterized by being prepared from the following raw materials in parts by weight: 20-26 parts of tremella auramtialba, 18-20 parts of passion fruit, 3-5 parts of ginseng, 2-3 parts of Szechwan goldthread root, 5-7 parts of sea cucumber, 2-4 pars of Tetrastigma hemsleyanum Diels et Gilg, 5-7 parts of turtle shell, 10-12 parts of golden cicada husk, 15-18 parts of sugarcane, 15-17 parts of flos puerariae, 16-19 parts of bamboo shoots, 17-19 parts of wheat, 18-20 part of fragrant rice, 19-21 parts of fragrant taro, 15-18 of fresh lotus roots, 4-5 parts of an additive and a proper amount of water. The prepared passion fruit healthcare beverage has magic effects of eliminating fatigue, refreshing, alleviating hangover, lowering lipid and blood pressure, diminishing inflammation, reducing speckles, protecting skin, keeping young, improving eyesight and removing internal heat as well as the effects of clearing heat, diminishing inflammation and relieving internal heat or fever and is capable of preventing nephritis, renal failure, uremia and the like.

The invention relates to a cuttage seedling culture method for root tuber early-growing of tetrastigma hemsleyanum. The method comprises the six steps: (1) selecting a local high-quality tetrastigma hemsleyanum variety; (2) determining a cuttage period; (3) collecting and treating cutting slips; (4) carrying out soil preparation and cuttage; (5) building a greenhouse and controlling light; (6) carrying out seedling stage management, which comprises the six substeps of watering, carrying out foliage application, weeding, carrying out new vine length fixing, carrying out pre-transplanting training and carrying out timely transplanting. After seedlings are cultured for 5-6 months by using the method, during transplanting, the ratio of the seedlings, of which the roots grow root tubers, reaches up to 75%, and the ratio of the seedlings with the root tubers is greatly increased; after the seedlings are cultured for 3-5 years, during harvesting, the root tubers are large and numerous, so that the grade and yield of the medicinal material are increased, tetrastigma hemsleyanum, namely a valuable medicinal material, of which wild collection is increasingly scarce can be effectively supplemented, a new technical scheme is provided for a newly-domesticated tetrastigma hemsleyanum seedling culture technology, and thus, the method has certain social and economic benefits.

The invention discloses a tetrastigma hemsleyanum Diels et Gilg twig single-bud cutting propagation method. The method comprises the following steps that 1, cuttings are cut, wherein annual tender branches of tetrastigma hemsleyanum Diels et Gilg are cut and are cut into single-bud cuttings with leaves, the length of the lower ends of the cuttings is 4-5 cm, and the upper ends of the cuttings are 2 cm away from single buds; 2, the cuttings are processed, wherein the cuttings are dipped in a rooting conditioning solution for 15-60 seconds, the concentration of the rooting conditioning solution is 50-150 mg/L, and the rooting conditioning solution is at least one of NAA, IBA and IAA; then the cuttings are put in a matrix through cuttage, wherein the matrix contains perlite and also contains at least one of turf and vermiculite, and the cuttage depth makes the single buds rightly exposed out of the matrix; 3, management is performed after cuttage, wherein timely spraying is performed for 30 minutes after cuttage. By adopting the method, the influence of the matrix, the types and combinations of hormones and treatment time on the survival rate of tetrastigma hemsleyanum Diels et Gilg single-bud twig seedlings raised by cuttage is researched, preferred cultivation conditions are provided for quick propagation of tetrastigma hemsleyanum Diels et Gilg seedlings, and sustainable supply of tetrastigma hemsleyanum Diels et Gilg resources is achieved.

The invention relates to rhododendron mucronulatum leaf tea for relieving exterior syndrome and clearing away lung-heat and a preparation method of the rhododendron mucronulatum leaf tea. The rhododendron mucronulatum leaf tea is prepared from tea leaves and a Chinese herb extract in a mass ratio of the tea leaves to the Chinese herb extract being 1 to 4, wherein the Chinese herb extract comprises the following raw materials in percentage by weight: 20-40% of rhododendron mucronulatum leaves, 10-20% of root tubers or herb of tetrastigma hemsleyanum, 10-15% of medicinal changium roots, 10-15% of succus bambusae, 5-10% of fructus momordicae, 5-10% of licorice roots, 5-10% of pinellia tuber leaven, 3-5% of tendrilleaf fritillary bulbs, 3-5% of semen sterculiae lychnophorae, and 3-5% of herba ephedrae. The preparation method of the rhododendron mucronulatum leaf tea comprises the main steps of a, preparing raw materials; b, performing extraction to obtain a filtrate; c, performing centrifugal separation and concentration; d, performing applying and performing drying by baking; and e, performing baking, frying and sterilization. After being drunk for a long term, the rhododendron mucronulatum leaf tea for relieving exterior syndrome and clearing away lung-heat, disclosed by the invention, has the efficacy of relieving exterior syndrome, clearing away lung-heat, dispelling wind and heat, clearing heat and removing toxicity.

The invention discloses preparation and application methods of a special selenium-enriched nutrient fertilizer for tetrastigma hemsleyanum. The preparation method uses waste stem leaves obtained in the process of artificial planting and processing of the tetrastigma hemsleyanum, sugar, seaweed, sea trash fish meal and whole wild tetrastigma hemsleyanum plants and water as raw materials, seal fermentation is performed to obtain a base liquid, and a specific ratio of alpha-naphthalene acetic acid, water-soluble chitosan, a selenium-rich nutrient solution and casein hydrolysate which are favorable for promoting the growth and development of the tetrastigma hemsleyanum are added into the base liquid, and therefore the special selenium-enriched nutrient fertilizer for the tetrastigma hemsleyanum is prepared; the nutrient fertilizer has a high selenium content, rich nutrients and no chemical fertilizers, contains specific symbiotic bacteria of roots of the whole wild tetrastigma hemsleyanumplants, is in line with the nutrient absorption and growth characteristics of the tetrastigma hemsleyanum, can promote growth of the tetrastigma hemsleyanum plants and greatly increase the yield and the content of medicinal active ingredients of the tetrastigma hemsleyanum, and makes the tetrastigma hemsleyanum rich in selenium, thereby realizing production and efficiency increase of the tetrastigma hemsleyanum; and the preparation and application methods disclosed by the invention are simple and practical, and have strong operability and low costs, thereby facilitating practical promotion andapplication.

The invention discloses an externally-applied traditional Chinese medicinal ointment for treating radiodermatitis. In the traditional Chinese medicine science, the property of radioactive rays belongs to heat; the radioactive rays are likely to damage a human body; the radiodermatitis is caused by excessive heat toxin, fire toxin acting on skin, and excessive heat induced flesh decay. Therefore, the externally applied traditional Chinese medicinal ointment is prepared from Chinese herbal medicines with functions of clearing away heat, detoxifying, cooling blood, nourishing blood, removing blood stasis and promoting tissue regeneration, including tetrastigma hemsleyanum diels et gilg, vernonia cinerea, Chinese mesona herb, lophatherum gracile, abrus cantoniensis hance, thesium chinense, brooklet anemone root, large-leaved cornel wood, spiral shell, rubus irenaus focke, cassie root and marigold. The ingredients are prepared to the externally-applied ointment by a manufacturing laboratory of the hospital. Through the clinical trial, the total effective rate is up to 96.9%, which is obviously better than the control group, so the externally-applied traditional Chinese medicinal ointment is worthy of clinical promotion and application.

The present invention discloses dolichos lablab l spleen strengthening health noodles, which are prepared from the following raw materials by weight: 70-80 parts of flour, 10-20 parts of common yam rhizome powder, 10-20 parts of mung bean powder, 10-15 parts of soybean protein powder, 8-10 parts of dolichos lablab l, 5-10 parts of tangerine leaf, 4-6 parts of licorice root, 8-10 parts of milkvetch root, 5-6 parts of flos citri sarcodactylis, 4-6 parts of dolichos lablab l flower, 4-6 parts of pueraria lobata, 4-6 parts of rose, 6-8 parts of cirsium setosum, 2-4 parts of lemon, 4-6 parts of artemisia integrifolia linn., 2-3 parts of tetrastigma hemsleyanum diels et gilg root tuber lyophilized powder, 0.1-0.5 part of spiral algae powder, 0.2-0.4 part of tea polyphenol, and 2-4 parts of a nutritional additive. The health noodles have characteristics of good taste, complete nutrition, boiling resistance, and refreshing and smooth taste, provide effects of blood fat reducing, spleen and stomach strengthening and constipation prevention after long term taking, and have the dietary therapy health effect, wherein the taste of the noodles is significantly improved with addition of the self-made nutritional additive.

The invention discloses a processing technic for tetrastigma hemsleyanum diels et gilg. The processing technic mainly comprises the following steps: naturally drying stems of the tetrastigma hemsleyanum diels et gilg, adding petroleum ether and performing reflux treatment; grinding and smashing leaves of the tetrastigma hemsleyanum diels et gilg and filtering; drying tuberous roots of the tetrastigma hemsleyanum diels et gilg in a neon atmosphere, cooling, drying again and then heating; drying fruits of the tetrastigma hemsleyanum diels et gilg, adding water for grinding, screening, performingreflux extraction for 6 times, filtering and drying filter residues; mixing the treated stem filter residues, leaf filtrate, tuberous root sheets and fruit filter residues of the tetrastigma hemsleyanum diels et gilg, uniformly smashing and grinding, and drying to obtain a tetrastigma hemsleyanum diels et gilg product finally. By adopting the processing technic, the problems of no pertinency in processing of the tetrastigma hemsleyanum diels et gilg, incapability of extracting effective components from each part of the tetrastigma hemsleyanum diels et gilg to the maximum extent, incapabilityof keeping the medicinal property for a long time, and difficulty in full absorption of the extracted effective components by human body are solved.

The invention discloses an ethanol extract ethyl acetate extractive fraction of tetrastigma hemsleyanum leaf and a preparing method and application thereof. The method includes: 1, crushing dried leaves of tetrastigma hemsleyanum to obtain dry powder, extracting with ethanol water, performing filtering, drying filtrate to obtain total extract of the tetrastigma hemsleyanum leaf; 2, mix-suspending the total extract of the tetrastigma hemsleyanum leaf and water, extracting with ethyl acetate to obtain ethyl acetate extract, and steam-drying the ethyl acetate extract to obtain the ethanol extract ethyl acetate extractive fraction of tetrastigma hemsleyanum. The ethanol extract ethyl acetate extractive fraction of the tetrastigma hemsleyanum leaf prepared by the preparing method is applicable to the preparation of antibacterial products, is effective in inhibiting germ growth and killing germs, applicable to drugs, cosmetics, health foods, foods and applicable to bacterial inhibition for the human, animals and the aquaculture process, and has a promising application prospect.

The invention discloses a cultivation matrix capable of helping the root tuber expansion of Tetrastigma hemsleyanum, and a preparation method thereof. The cultivation matrix is prepared from a nutrition matrix, bamboo leaf (or tree leaf) and forestry land topsoil according to a mass ratio of 1:2:10-1:5:30, wherein the nutrition matrix comprises, by mass, 40-60 parts of fermented sheep manure (or sheep manure or rabbit manure), 15-20 parts of peat soil, 5-10 parts of lime powder, 5-10 parts of plant cake meal, 5-10 parts of hard particles for stimulating the root tuber forming of roots, and 5-10 parts of calcium superphosphate, wherein sheep manure (or sheep manure or rabbit manure) and plant ash are mixed according to a mass ratio of 7:3 and stacking fermentation is performed for 20-40 days to prepare the fermented sheep manure (or sheep manure or rabbit manure), stack flipping is performed 3-6 times during the fermenting after the temperature of the fermenting stack is increased to more than 65 DEG C so as to achieve a constant temperature, and after the fermenting is completed, spreading drying in the sun is performed to reduce the water content to 20-30%. With the cultivation matrix of the present invention, the yield of the root tuber of Tetrastigma hemsleyanum can be increased, the environment can be protected, and the cost is low.

The invention discloses an induction and propagating method of a Tetrastigma hemsleyanum Diels et Gilg adventitious root, comprising the following steps of (1) induction of callus: cleaning leaves picked from a Tetrastigma hemsleyanum Diels et Gilg plant, sterilizing the leaves with ethyl alcohol on an ultra-clean workbench, then sterilizing the leaves with mercury bichloride, washing with sterile water, cutting, horizontally placing on an inducing medium with the right sides of the leaves being upward, and performing dark culture at 27+/-1 DEG C; (2) propagation of the callus: cutting the callus obtained in step (1) into small pieces on the ultra-clean workbench, inoculating the cut callus with a propagation medium, and performing dark culture at 27+/-1 DEG C; (3) induction of callus differentiation adventitious root: cutting the callus obtained in step (2) into small pieces on the ultra-clean workbench, inoculating the cut callus with an adventitious root induction medium, and performing dark culture at 27+/-1 DEG C; (4) propagation of the adventitious root: cutting the adventitious root obtained in the step (3) into the set length on the ultra-clean workbench, inoculating the cut adventitious root with a liquid culture medium of propagation of the adventitious root, performing dark culture, and then performing light culture.